자료포락분석(DEA)를 이용한 보건의료의 효율성 측정

김남송,박홍섭,김은진,김설화,김정숙,김현아,김경숙,신지혜,최은정,양은진,유선미,유수진 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

신경병증성 통증 모델쥐 뒤뿌리신경절의 Nitric Oxide Synthase 아형 및 NADPH-diaphorase 발현에 관한 연구

정성균,김수일,양은진,류택근,권오유,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Injury to peripheral nerve somtimes lead to neuropathic pain. Nitric oxide(NO) is increased in the dorsal root ganglia (DRG) of neuropathic pain models, and L-NAME, a nitric oxide synthase (NOS) inhibitor, reduces neuropathic pain. However, it is not known that underlying mechanism of NO involvement in development and maintanence of neuropathic pain. It was found that there were three NOS isoforms-nNOS, eNOS, and iNOS. It is also necessary to investigate NOS isoforms in neuropathic pain. The aim of this study is to investigate NOS isoforms and NADPH-diaphorase expressions and underlying mechanism of NO involvement in neuropathic pain. The 5^th lambar (L5) DRG were obtaind after tight ligation of L5 an L6 spinal nerves for 7 days. Toluidine blue stain, immunohistochemistry for nNOS, eNOS, and iNOS, and NADPH-diaphorase histochemistry were performed. All specimens were observed under light microscope. nNOS expression was increased significantly in the cytoplasm of the DRG of ligated side at 7 day post-ligation (7D PL). eNOS expression was also increased in the blood vessel, a few neurons or satellite cells, and cell membrane of Schwann cells in the DRG of ligated side at 7D PL. iNOS expression was increased in a few blood vessels and cytoplasm of small neurons in the DRG of ligated side at 7D PL. NADPH-diaphorase expression was increased significantly in the neurons of the DRG of ligate side at 7D PL, but NADPH-diaphorase expressions were weak or absent in the blood vessel and Schwann cell in the contralateral DRG and the DRG of ligated side at 7D PL. It is suggested that NOS isoforms may be closely related to development and mantanence of neuropathic pain in order of nNOS>eNOS>iNOS.

폐암으로 오인된 이물질에 의한 기관지 육아종증 1예

정종훈,김소영,김휘정,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

고추 탄저병 저항성 중간모본 ‘원예 100005호 ’,‘원예 100006호’ 육성

양은영,김수,채영,채수영,조명철,문지혜,박태성,이우문,곽정호 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Owing to frequent heavy rain due to abnormal weather, the occurrence of pepper anthracnose has recently increased, which affects the supply and demand of pepper. As a result, the price of red pepper powder in 2011, for example, increased three times compared to the average year. Although anthracnose of pepper plants has been controlled by fungicides until now, it is important to develop new resistant cultivars because of the reduction of the labor force in rural area and occurrence of new anthracnose strains resistant to fungicides. National Institute of Horticultural and Herbal Science developed two pepper lines, ‘Wonye 100005’ and ‘Wonye 100006’ that are resistant to pepper anthracnose. These lines were developed by continuous selection of progenies from a cross between ‘Wonye AR1’, which were developed from interspecific hybridization and ‘TF68’. The anthracnose resistance level of the two lines was higher than that of commercial cultivars and similar to check varieties showing highest level of anthracnose resistance.

하이퍼텍스트 유형과 자기주도성이 학업성취에 미치는 효과

박정환,양은영 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.4

본 연구의 목적은 하이퍼텍스트 유형과 자기주도성이 학업성취에 미치는 효과를 규명하려는 것이다. 이를 위해 경기 H시 소재 H고등학교 1학년 2학급 69명을 대상으로 자기주도성 검사를 실시하여 평균점수 이상을 얻은 학생을 자기주도성이 높은 집단으로, 그 미만을 얻은 학생을 자지주도성이 낮은 집단으로 분류하여 실험처치를 하였다. 연구 결과, 하이퍼텍스트 유형은 학업성취에 영향을 미치지 않았으나 학습자의 자기주도성은 학업성취도에 영향을 미치는 것으로 나타났다. 또한 하이퍼텍스트 유형과 학습자의 자기주도성이 학업성취도에 미치는 상호작용 효과가 있는 것으로 밝혀졌다. 본 연구는 비교적 짧은 기간에 이루어져 지속적인 학습효과를 검증하는데 한계가 있기 때문에 장기간에 걸친 연구가 필요하다. 또한 자기주도성 이외의 학습자 특성을 고려한 연구와 컴퓨터일반 교과 외 다양한 학습내용으로 하는 후속 연구가 필요하다. The purpose of this study was to find effects of hypertext structure and self-direction on learning performance. To reach the goal, we got 69 H highschool students and tested self-direction we treat hypertext program to them. The results found were that hypertext style(non-structure, structure) didn’t effect on academic achievement but did effect learner’s self direction. Also, we found interactive effect of hypertext structure and self-direction on academic achievement. Following studies have limits verified learning effect, because this studies are carried out in short-term research. Further studies need the study of effect of hypertext structure and self-direction on academic achievement for a long-term research. Also, further studies should accomplish the study considering learner characteristics besides self-direction and will need succeeding studies including various learning contents besides computer general curriculum.

교잡종 찰옥수수에 대한 연구 III : Ⅲ. 국내 옥수수 유전자원 수집종에 대한 주요 특성 분포

서정은,주현민,최윤표,홍범용,조양희,주정일,지희정,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

2006년 국내 각 지역으로부터 수집된 재래종옥수수 유전자원을 관행방법에 의해 파종하고 주요특성별로 분포도를 살펴본 결과는 다음과 같다. 1. 수집된 유전자원에 대한 발아율은 대부분 80∼100%이상의 분포를 보였으나, 일부 수집계통에는 30% 미만의 극히 저조한 발아율을 보였다. 2. 수집자원에 대한 초기생육을 살펴보면 극히 생육이 저조한 것이 40% 정도를 보였고, 극히 우수한 자원은 3%미만으로 나타났다. 3. 간장은 대부분이 150∼199cm범위에 55%가 분포하고 있었으나, l00cm이하가 5%, 250cm이상이 8%로 나타나 수집종간에 큰 차이를 보였다. 4. 주당 분얼수는 1개 미만이 40%의 분포를 보였으나, 일부 수집종에서 2 내지 3개의 분얼수를 보이는 계통이 확인되었다. 5. 개화소요일수는 61∼64일의 범위에서 40%, 57∼60일의 범위에서 30%의 분포를 보였으며, 특히 일부 수집종의 경우 55일 이하의 극조생종도 10% 정도의 분포를 보였다. 6. 이삭길이는 대부분 11∼15cm범위에 60%가 포함되어 있으나, 일부계통에서 5cm미만이 2%, 16∼20cm의 이삭길이도 10%가 분포하였다. Germination ratio on the 300 collected genetic resources showed 80 to 100 percentage, but those of some accessions were very low as below 30 percentage. Early growth phase of most of these lines were very poor while some collected lines were very vigor. Range of stem height showed from 150 to 199cm, but that of some accessions showed below 100cm as five percentage and some of them was over 250cm as eight percentage. Number of tillers per plant appeared less one but some collected lines have two to three tillers per plant. In days to tasseling, some accessions showed more four per plant. Ear length of these accession showed above 60 percentage as 11 to 15cm, while that of some collected lines showed over 10 percentage as 16 to 20cm.

건조방법에 따른 두부의 품질특성

이정은,이현규,김용찬,양차범 漢陽大學校 韓國生活科學硏究所 2001 韓國 生活 科學 硏究 Vol.- No.19

In order to compare the quality properties with drying methods, soybean curd were studied through hot-air drying, vacuum drying and freeze drying methods. The color(lightness and ΔE) was decreased by drying, and showed similar trend to drying methods. However, hot-air drying showed the greatest change in browning and protein denaturation degree. Also, the hardness after rehydrated was the highest in hot-air drying. From the results, the quality properties of soybean curd were the most decreased by hot-air drying, and vacuum drying and freeze drying methods were similar changed.

중안면부 함몰과 하악전돌을 동반한 Ⅲ 급 부정교합자의 교정-악교정수술 복합치료

조은정,김종태,양원식 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5

성장이 완료된 골격성 III급 부정교합의 치료는 골격 부조화의 심도, 절치 치축, overbite와 연조직 측모의 심미성 등을 고려하여 필요한 경우 교정치료와 악교정수술을 병행하여 골격과 치아관계 개선에 따른 기능의 증진외에도 안모의 심미성을 증진시킬 수 있다. 특히 전후방적, 수직적, 횡적인 골격 부조화가 크거나 안면비대칭이 있는 경우, 또한 악교정수술후의 안정성이나 보다 이상적인 안모를 위하여 양악수술을 시행한다. 중안면부 함몰환자에서 안와하연과 관골부는 보존하며 비상악체를 전방이동하는 pyramidal Le Fort II osteotomy를 시행할 수 있으며, 안와하연과 관골부의 전방이동을 필요로 할 때는 quadrangular Le Fort II osteotomy를 시행할 수 있다. 이 수술법은 안와하연의 위치를 변화시켜 안와체적에 영향을 미치므로 상악골의 횡적, 수직적 이동량이 5mm 이상일 때는 시행할 수 없다. 서울대학교병원 치과진료부 교정과에 내원하여 중안면부 함몰과 하악전돌을 골격성 III급 부정교합으로 진단되어 교정-악교정수술(Le Fort II osteotomy 와 BSSRO) 복합치료를 받은 2명의 환자에 대해 살펴보고자 한다. In non-growing Class III malocclusion, the critical aspects which determine the need of orthognathic surgery are the severity of skeletal discrepancy, incisor inclination, overbite and soft tissue profile. Two-jaw surgery is more effective in correcting severe sagittal, vertical, transverse skeletal discrepancies and facial asymmetry. And more esthetic and stable profile can be achieved by two-jaw surgery. Some midfacial deficiency patients can be treated by pyramidal Le Fort II osteotomy to maintain infraorbital rim and malar complex and to advance nasomaxillary complex. Others who require advancement of infraorbital rim and malar complex can be treated by quadrangular Le Fort II osteotomy. On the following cases, patients who had represented midfacial deficiency and mandibular prognathism were treated with combined orthodontic-surgical therapy by Le Fort II osteotomy and BSSRO.

방광의 이행상피암에서 p53과 PCNA 분포에 관한 연구

최은정,강상균,양승하,김대중,김의한 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

Immunohistochemical studies were carried out to observe the expression of p53 protein and PCNA in transitional cell carcinoma (TCC), then analyzed according to the tumor grade and progression. 38 cases of TCC consist of 13 cases grade Ⅰ, 12 cases grade Ⅱ and 13 cases grade Ⅲ respectively. Results obtained are as follows. 1. p53 protein expression was seen in 60% of cases(15 out of 38 cases) In the poorly differentiated area and infiltrated lesion, tumor cell showed more strong positive reaction of p53 protein compared with well differentiated area. 2. All of 38 cases of TCC showed PCNA positive expression. The more strong positivity was seen in the poorly differentiated area and the more intense positive reaction was noted in the basal layer of the tumor nests. 3. The above results showed that positivity of p53 protein and PCNA are closely related with tumor cell differentiation(grade) and progression. It is concluded that positive reaction of p53 protein and PCNA may contribute to detection of tumor growth and prognosis of patient.

S. cerevisiae에서 세포주기의 진행에 관련된 COS28과 COS46 유전자의 클로닝

박정은,임선희,선우양일 동아대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.17 No.1

본 연구에서는 효모의 돌연변이주를 분리하여 G1/S기에서 세포주기 진행에 관련된 유전자의기작을 이해하기 위해 연구를 수행하였다. 먼저, cos28과 cos46 돌연변이주의 MMS(methylmethane sulfonate)와 HU(hydroxyurea)에 대한 감수성을 조사하였다. 이들 돌연변이주들은 MMS와 HU에 대해서 감수성을 보이므로, S기 checkpoint에 관련되었다고 생각된다. 다음으로 cos28과 cos46 돌연변이를 상보하는 유전자를 클로닝하였고, 서열화하였다. 그리고 클론된 유전자는 데이터 베이스 분석을 통해 조사하였다. cos28 변이주를 상보하는 DNA 단편은 전체 6개의 ORF를 가지며 그 중 기능이 알려진 ORF로는 RPS21B와 LCB3와 MRS3가 있고, 기능이 알려지지 않은 ORF로는 YJL131와 YJL132와 YJL135의 세개를 포함하고 있었다. cos46 유전자는 전체 6개의 ORF를 가지며, 그 중 기능이 알려진 CYP5와 HNT2와 SRB7와 GIC2를 가지고, 기능이 알려지지 않은 ORF로 YDR306, YDR307를 포함하고 있음이 확인되었다. In this study, to understand the mechanisms of genes which related in cell cycle progression at G1/S phase, using the isolated mutants in S. cerevisiae were investigated. First of all, cos28 and cos46 were examined the sensitive to MMS(methylmethane sulfonate) and HU(hydroxyurea). These mutants were showed MMS, HU sensitivities and might be a act at a checkpoint pathway during S phase. These mutants were carried out gene cloning for the gene which were complemented the cos mutants. As a result, the DNA fragments which complement with the cos28 and cos46 mutants, were cloned and sequenced. And then cloned genes were surveyed though data base analysis. Complemented DNA fragment of COS28 contained six ORF which were consisted RPS21B, LCB3, MRS3, YJL131, YJL132, and YJL135. Complemented DNA fragment of COS46 contained six ORF which were consisted CYP5, HNT2, SRB7, GIC2, YDR306 and YDR307.