Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene

Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.

Cloning, Expression, and Functional Characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate Synthase Gene in Escherichia coli

Yi, Yi,Qiao, Dairong,Bai, Linhan,Xu, Hui,Li, Ya,Wang, Xiaolin,Cao, Yi The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

Comparative Analysis of the Genomes of Bombyx mandarina and Bombyx mori Nucleopolyhedroviruses

Yi-Peng Xu,Zheng-Pei Ye,Chang-Ying Niu,Yan-Yuan Bao,Wen-Bing Wang,Wei-De Shen,Chuan-Xi Zhang 한국미생물학회 2010 The journal of microbiology Vol.48 No.1

The Bombyx mandarina nucleopolyhedrovirus (BomaNPV) S1 strain can infect the silkworm, Bombyx mori,but is significantly less virulent than B. mori nucleopolyhedrovirus (BmNPV) T3 strain. The complete nucleotide sequence of the S1 strain of BomaNPV was determined and compared with the BmNPV T3 strain. The circular, double stranded DNA genome of the S1 strain was 126,770 nucleotides long (GenBank accession no. FJ882854), with a G+C content of 40.23%. The genome contained 133 potential ORFs. Most of the putative proteins were more than 96% identical to homologs in the BmNPV T3 strain, except for bro-a, lef-12,bro-c, and bro-d. Compared with the BmNPV T3 strain, however, this genome did not encode the bro-b and bro-e genes. In addition, hr1 lacked two repeat units, while hr2L, hr2R, hr3, hr4L, hr4R, and hr5 were similar to the corresponding hrs in the T3 strain. The sequence strongly suggested that BomaNPV and BmNPV are variants with each other, and supported the idea that baculovirus strain heterogeneity may often be caused by variation in the hrs and bro genes.

A Comparison of Transradial and Transfemoral Percutaneous Coronary Intervention in Chinese Women Based on a Propensity Score Analysis

Yi Xu,Chen Jin,Shubin Qiao,Yongjian Wu,Hongbing Yan,Kefei Dou,Bo Xu,Jingang Yang,Yuejin Yang 대한심장학회 2018 Korean Circulation Journal Vol.48 No.8

Background and Objectives: Over the past decades, transradial approach for percutaneous coronary intervention (PCI) has been increasingly adopted in clinical practice. Women represent a large population who will possibly benefit from PCI, but they are often under-represented in clinical studies. Therefore, the role of TRI in women remains to be further defined. This study sought to compare safety and efficacy for transradial intervention (TRI) and transfemoral intervention (TFI) in women undergoing PCI in China. Methods: The study population consisted of 5,067 women undergoing PCI at Fuwai Hospital, Beijing, China between 2006 and 2011 (TRI: n=4,105, TFI: n=962). Incidence rates of clinical outcomes during hospitalization and at 1-year follow-up were compared between TRI and TFI. In order to minimize potential biases, a 1:1 propensity score matching (PSM) was performed. A total of 899 pairs were matched. Results: Baseline and procedural characteristics were well-balanced between TRI and TFI groups after controlling for confounders using PSM. TRI was associated with reduced major post-PCI bleeding (odds ratio [OR], 0.64; 95% confidence interval [CI], 0.54–0.76; p<0.001) and access site complications (OR, 0.67; 95% CI, 0.61–0.74; p<0.001) after PSM. There was no statistical differences in the incidence rates of major adverse cardiac events (a composite of cardiac death, myocardial infarction, and target vessel revascularization) both during hospitalization and at 1-year follow-up (p>0.05). Conclusions: In this propensity score-based analysis of TRI versus TFI in Chinese women, TRI showed advantages of safety and feasibility over TFI. A wider adoption of TRI in women has the potential to improve outcomes in treatment of coronary artery diseases.

十八世紀朝淸文士筆談內容考述

( Yi Xu ),( Li Chen ) 한국외국어대학교 중국연구소 2014 中國硏究 Vol.60 No.-

The 18th century is a period when Qing and Choson literati communicate frequently with a variety of communicating forms such as conversations by writing, the exchange of poetry, reciprocating letters, asking for providing preface and postscript, literary criticism as well as giving books as presents, among which the conversations by writing is the most popular way of interaction. According to statistical study by the author, at least 193 detailed conversations by writing happened in the 18th century were recorded in the extant Yeonhaeng Rok, one of Chinese and Korean ancient scriptures. Its contents mainly covered ten aspects, in which some in-depth exchange was recorded. Therefore, these materials are of great literature value. Regarding to the communication of etiquette system and custom, Qing people`s comments on Choson ambassadors` clothing and inturn Choson views of binding feet of Qing women are always the key points. Besides, it also includes the communication of imperial examination system in Qing Dynasty and Ancient Korea;the discussion about academy and literary between Qing and Choson scholars; Choson literati` concerns with religion; Choson literati` focus on the Ming historical events, Qing political and agricultural situation as well associal development; Choson literati` acquaintance with compilation of books in Qing Dynasty, for example "The Imperial Collection of Four Treasures";the exchange of banned books in Qing Dynasty between Qing and Choson literati; Choson scholars` care for the landscape, geography of China and places of interest in China. Qing people also pay attention to Choson in many regards, for instance, landscape and geographical situation, scene style and feature views, ancient authors and their works, history, etiquette, custom, education, languageetc. It is obvious to find the diversity of their conversation topics. And these topics are closely associated with the culture, custom, history and the focus on Confucianism of Qing Dynasty and Choson. It should also be emphasized that no matter how abundant the conversations are, the key point is always related to concerns of Choson literati when visiting Qing Dynasty and their own scholar traits.

The Effects of Allitridin on the Expression of Transcription Factors T-bet and GATA-3 in Mice Infected by Murine Cytomegalovirus

Xu Yi,Fang Feng,Zhidan Xiang,Li Ge 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3

This study was designed to investigate the effects of allitridin on the expression of transcription factors T-betand GATA-3 in mice infected by murine cytomegalovirus (MCMV). A BALB/c mouse model system of MCMV infectionwas established. Twenty mice were allocated randomly into an allitridin-treated group (n . 10) and a placebo control group(n . 10). The same dose (25 mg/kg/day) and regimen of allitridin were used in the treated group in the 24 hours after virusinfection; the same volume of saline solution was injected in placebo control mice. In an additional blank control group (n .10), the same volume of saline solution was injected. The expression levels of the transcription factors T-bet and GATA-3were measured by reverse transcriptionpolymerase chain reaction. The expression levels of the T helper (Th) 1 cytokine in-terferon-. (IFN-.) and the Th2 cytokine interleukin (IL)-10 in supernatant of spleen cell culture were measured by enzyme-linked immunosorbent assay. MCMV infection markedly down-modulated the expression of IFN-. and T-bet and significantlyup-modulated the expression of IL-10 and GATA-3. Allitridin induced significantly (P. .01) increased expression of thetranscription factor T-bet and the Th1 cytokine IFN-. and markedly (P. .01) decreased expression of the transcription fac-tor GATA-3 and the Th2 cytokine IL-10. Thus MCMV infection could lead to disequilibrium of Th1/Th2 cytokine expres-sion: The level of the Th1 cytokine IFN- was decreased significantly, and Th2 cytokine IL-10 was overexpressed markedly.Allitridin could up-regulate the expression of T-bet and IFN-. and inhibit the expression of GATA-3 and IL-10 in MCMV-infected mice, indicating a Th1 dominant state, which should enhance the specific cellular immune reactions against cy-tomegalovirus (CMV) and be helpful for clearance of CMV from the host.

朝鮮使者眼中的淸代戱曲

( Yi Xu ) 연민학회 2010 연민학지 Vol.14 No.-

본고는 조선시기 『燕行錄』중의 희곡 관련 자료를 연구대상으로 하여, 이국자의 시각으로 본 청대 희곡의 연극 활동 상황을 고찰하여 본다. 본고는 우선 『燕行錄』중에 청대 희곡의 연출 환경에 대한 묘사를 검토 분석하고, 그 다음 희곡 연출 복식에 대한 조선 사신들의 관심에 대해 논술하고, 마지막으로 『燕行錄』에 기록된 희곡 극목에 근거하여 청대 민간 희곡과 궁정 희곡의 분야를 서술해보려고 한다. 이런 보기드문 희곡 자료에 대한 정리를 통해 청대 희곡 연구의 시야를 넓혀보려고 한다. The research object of this paper is the Chinese Opera materials that are contained in YanXingLu (Travelogues of Korean Envoys to China). The activities of the Chinese opera are depicted through the perspective of Li Dynasty`s scholars. Firstly, I will analyze the environment of the performance recorded by the Korean envoys. Secondly, I will explain the reason that Korean scholars pay attention to the costume of the Chinese Opera. Finally, I will illustrate the differences between the folk drama and the palace opera. We could expand our research sight on the Qing Dynasty Opera by analyzing these ancient art works.

Framing advocacy event: Comparing news coverage and Facebook comments of the Belt and Road Forum in Pakistan and the USA

Xu, Yi World Association for Triple Helix and Future Stra 2021 Journal of Contemporary Eastern Asia Vol.20 No.1

With regard to the recent developments in public diplomacy, the increasing fusion of strategic communication appears necessary. China engages in public diplomacy with a strategic purpose to shape its national image abroad. Hosting diplomatic advocacy event is regarded as an instrument with expectations to present reliable and responsible image and promote international collaborations. The present research focuses on the Belt and Road Forum (BRF) in May 2017 with the objective to analyze its outcomes and influence on the international news agenda, news frames, and foreign citizens' comments online. The quantitative content analyses are used to compare the media reports (N=364) and Facebook users' comments on the selected news (N=957) between the US and Pakistan. Results reveal that Pakistani media provided more diverse frames and attributed more positive evaluations to the BRF than the US media. However, Facebook comments expressed more unfavorable opinions toward the BRF and China's image with rare differences between two countries. In conclusion, the BRF has served as an eye-catching advocacy of Chinese foreign policy, as it influenced the news agenda in two selected countries. However, news frames vary due to the differences in media system and the involvement in the BRF. China's public diplomacy practices follow a traditional top-down communication which needs meticulous subdivision of target stakeholders, delicate messaging strategies, and integrated tactics.

Influence of Hybrid Ratios on Shape Memory Properties of Basalt/Carbon Fiber Hybrid Composites with Graphene Oxide Prepared by VIHPS

Yi Xu,Yuqin Ma,Yu Yang,Gangfeng Wang,Wei Xu,Fei Li,Haiyin Guo,Yatao Li 한국섬유공학회 2023 Fibers and polymers Vol.24 No.8

A study on the shape memory properties of 6 layers bi-directional basalt fiber/carbon fiber hybrid composites with graphene oxide (GO) was presented in this paper. The hybrid composites were prepared by the vacuum infiltration hot pressing system (VIHPS). The macroscopic appearance of these composites was desirable, and their microstructure was satisfactory. According to the test results, with the content of basalt fiber decreased, the shape fixation ratio decreased, while the shape recovery ratio and shape recovery force all increased. The basalt fiber composite with 6 layers basalt fiber (B6) had a maximum shape fixation ratio of 94.94%, a minimum shape recovery ratio of 85.52%, and a minimum recovery force of 1.36 N. Compared with B6, the shape fixation ratio of the carbon fiber composite with 6 layers carbon fiber (C6) decreased by 10.43%, which was 84.51%. The shape recovery ratio and shape recovery force were increased by 9.60% and 3.31 times, and their values were 95.12% and 5.86 N, respectively. In addition, according to the temperature-recovery force curves, it was found that the seven groups of composites all reached their maximum shape recovery force when the temperature was about 110 °C. When the temperature was between 50 °C and 80 °C, the recovery force of the composite increased greatly, and then, the recovery force increased slightly. However, when the temperature was above the glass transition temperature (Tg), the recovery force gradually decreased.

Enhanced Photocatalytic Performance of Heterojunction BiOI/BiOIO3 Nanocomposites Under Simulated Solar Light

Yi Ling Qi,Xu Chun Song,Yi Fan Zheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

Novel heterostructure BiOI/BiOIO3 nanocomposites were successfully prepared through a facile deposition method at room temperature. BiOIO3 is a noncentrosymmetric compound that has an internal self-built electric field. BiOI was applied as a visible light absorber to sensitize semiconductors owing to its smallest bandgap. The coupling between BiOIO3 and BiOI can combine their advantages and improve photocatalytic properties. Compared with the single BiOI and BiOIO3, the heterostructure BiOI/BiOIO3 nanocomposites displayed a significantly enhanced photocatalytic activity for the Rhodamine B (RhB) degradation. The enhanced photocatalytic performance is deduced closely related to the formation of BiOI/BiOIO3 heterojunction interface whose presence is regarded to be a favorable factor for the transfer and separation of the photogenerated electrons and holes.