Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

曲管덕트 입구 영역에서 遷移定常流動의 速度分布와 壁面剪斷應力分布에 관한 연구

손현철,김명호,박길문 朝鮮大學校 機械技術硏究所 1998 機械技術硏究 Vol.1 No.1

In the present study, the flow characteristics of developing transitional steady flows in a square - sectional 180° curved duct are investigated experimentally. The experimental study for air flow in a square - sectional 180° curved duct is carried out to measure the axial velocity profiles and wall shear stress distributions by using Laser Doppler Velocimeter (LDV) system with data acquisition and processing the system of FIND software at 7 sections from the inlet (ø=0°) to the outlet (ø=180°) in 30° intervals of a curved duct. The results obtained from experimental studies are summarized as follows : (1) The critical Dean number of transitional steady flow is about 450. They show that critical Reynolds number in a curved duct is larger than that in a straight duct. (2) For transitional steady flows, the maximum velocity position of axial velocity profiles begins to incline toward the outer wall from ø=30°bended angle, velocity profiles in center of the duct have lower value than those of the inner and outer walls because of the centrifugal forces. (3) Wall shear stress distributions of transitional steady flows are increased at the entrance region because of increasing shear stress and pressure loss by the influence of flows.

요부 외측구역 협착증에서 단순감압술과 기구고정술을 병행한 요추체간 골유합술과의 임상적 비교

손병길,최은석,정을수,신종현,김명진,지용철 대한척추신경외과학회 2004 Neurospine Vol.1 No.1

Objective: The lumbar spinal canal can be devided into the central zone and the lateral zone. The divisions of the lateral zone are the subarticular, the foraminal, and the extraforaminal zone. A common causes of the operation for the spinal stenosis are related to the association with the spondylolisthesis and the central stenosis. There is a paucity of data, however, regarding the operative intervention in the lateral zone stenosis(LZS). Our aim was to analyze the surgical result by the anatomical type and the operative method. Methods: 72 patients of LZS excepting the association with the spondyolisthesis have been operated by six neurosurgeons of our hospital from 30th April, 1994 through 30th September, 2002. The patients had been interviewed about surgical result through telephone by third party persons. The surgical results were analyzed by the four score rating system: ① medication, ② ADL(Ability of daily living), ③ the degree of improvement, ④ the degree of satisfaction. Results: Among 72 patients, there were 25 males and 47 females. The age group of the patients were in their thirties(1), forties(9), fifties(21), sixties(36) and seventies(5). Surgical methods were used on decompression(22), posterior lumbar interbody fusion(PLIF) with cage(1) and PLIF with transpedicular screw fixation system(49). The types of stenosis were subarticular(24), foraminal(16) and extraforaminal(30) and double crush(2). The overall surgical results were Excellent(18), Good(34), Fair(19), and Poor(1). The Non-success rate according to the methods of surgery were decompression(34.8%), PLIF with transpedicular fixation system(24.4%). The overall success rate was(53/72: 73.6%). The success rate according to the types were subarticular(17/24: 70.8%), extraforaminal(21/30: 70%), and foraminal(12/16: 75%). Conclusion: It is suggested that the result was more favorable on the cases of lateral zone stenosis operated with PLIF and transpedicular fixation system. According to the method used, the success rate of decompression was worse than PLIF with transpedicular fixation system.

곡관덕트에서 난류정상유동의 벽면전단응력분포와 입구길이에 관한 실험적 연구

손현철,이홍구,김대욱,김명호,박길문 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

In this paper, an experimental investigation of characteristics of developing turbulent steady flows in a square-sectional 180°curved duct is presented. The experimental study using air in the square-sectional 180°curved duct is carried out to measure entrance length and wall shear stress distributions by using Laser Dopper Velocimeter(LDV) system with data acquisition and processing the system of FIND6260 software at 7 sections from the inlet(ø=0°) to the outlet(ø=180°)in 30°intervals of the curved duct. The results obtained from experimental studies are summarized as follows : (1) The critical Dean number of turbulent steady flow is about 560. (2) The value of the wall shear stress becomes minimum in the inner wall and gradually increases toward the outer wall where it becomes maximum. (3) The entrance length of the turbulent steady flow is shorter than that of the traditional steady flow, reaching the vicinity of bend angle 90°

상아질 접착제의 중합 시간 조절에 따른 복합레진의 중합 수축 방향의 변화

배지현,오명환,김창근,손호현,엄정문,조병훈,권혁춘 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.4

The purpose of this study was to evaluate the effect of adhesive curing timing on the direction of polymerization shrinkage of light-curing composite resin. In this study, the curing times of adhesive and composite resin were measured by differential scanning calorimeter(DSC). 28 extracted human molars were embedded in clear resin and box-type cavities were prepared. Based on DSC data. the experimental teeth were divided into 4 groups. Group 1: no bond: Group 2: late curing: Group 3: Intermediate curing: Group 4: Early curing. After treating with adhesive, the buccal cavities were filled with Z-100 hybrid composite resin and the lingual ones were filled with AEliteflo flowable composite resin. The depressions at the surface were measured by surface profilometer, then the specimens were embedded in clear resin and sectioned. Impressions were obtained and used to get epoxy resin replicas. The epoxy replicas were gold-coated and observed under SEM. Average Maximum Gap(AMG), Gap Proportion(GP), Average Marginal Index(AMI) were used to compare the shrinkage gap of each group. The results were statistically analyzed using the Kruskal-Wallis One Way ANOVA, Student-Newman-Keuls method. The results of this study were as follows. 1. Average Maximum Gap. Gap Proportion, Average Marginal Index and depression at the surface of Z-100 hybride composite resin were smailer than those of AEliteflo flowable composite resin(P(0.05) 2. When the bonding between composite resin and tooth structure was strong, the shrinkage gap was small, and depression at the surface was deep(P<0.05). 3. In the well-bonded group. light-curing composite resin shrank toward bonded cavity wall. not toward light source. The result suggested that the direction of polumerization shrinkage was affected by the quality of bonding in the dentin-resin interface. The strong was the bonding between composite resin and tooth structure, the smaller was the gap and the deeper was the depression at the surface. Then the flow to compensate the polymerization shrinkage proceeded from surface to bonded cavity wall.

자살기도자의 임상적 특성

전현수,손명세,한선호 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.2

The purpose of this study was to investigate the clinical meanings and the various factors associated with the patients who attempted suicides. The study subjects were 59 patients who made suicidal attempt(18 males and 41 females) and were treated in the neuropsychiatric department of Soonchunhyang University Hospital from Jan. 1979 to May 1986. From the analysis of the medical records of the patients, the following results were obtained. 1) The male ; female sex ratio of the patients was 1 : 2.3. 2) 50.9% of the patients were in the age group of 20-29. In the male patients, the age group of 16-19 was the most prevalent, whereas in the female patients, the age group of 20-29 was the most prevalent. 3) 54.2% of them were in the middle class of socioeconomic level and 86.4% of the patient had more than high school education. 4) In terms of occupation, 37.2% of the patients were unemployed, 25.4%, students, 15.3%, housewives, 6.8%, salary men, and 5.1%, business men. 5) The marital status ; 61.0% of the patients were singles, 25.4%, married 5.1%, separated, 5.1%, widowed, and 3.4%, divorced. 6) The sibling orders : 44.1% of them were the middle children, 28.8%, the first children and 27.1%, the last. 7) Previons suicidal attempt : 74.5% of the patients made no previous attempt, 11.9%, once before, 8.55, two times, 5.1%, 3 times or more. 8) 72.8% of the patients attempted suicide in their own home. 9) As method of suicidal attemps, 74.5% of the used drugs. 54.1% used tranquilizers alone, 6.8%, tranquilizers and alcohols, 5.1%, rat poisons and tranquilizers, and 1.7%, insect killers. 10) The motives of suicidal attempt : 44.0% of the patients had a conflict with lovers or spouses, 22.0%, mental disorders, and15.3, family discord. Job and business problems were the cause exclusively for male patients whereas pessimism and debt were limited to the female patients. 11) The distribution of psychiatric diagnosis : 30.5% of the patients had adjustment disorders, 8.5%, schizophrenia, 6.8%, major depression, 5.1%, bipolar disorders, depressed, and 1.7%, alcoholic hallucinosis. In the male patients, the order of diagnostic distribution was as follows, personality disorders(10.1%), adjustment disorders with depressed mood(5.1%), schizophrenias (5.1%), whereas in the female patients, adjustment disorders with depressed mood was followed by personality disorders (17.0) and dysthymic disorders (15.2%). 12) The mode of hospitalization : 45.7% of the patients were transferred from other departments, 39.0% of them were hospitalized via emergency room, and 15.3%, via out-patient department. Internal medicine department was the most common route of transfer.

안면부, 유방 및 상완부에 발생한 홍반성 루푸스 지방층염 1예

송유근,손두식,명형준,이현정,임중규,김순호,김윤아 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.2

Lupus erythematous panniculitis is a chronic inflammatory disease of subcutaneous fat that occurs in 2-3% of patients with systemic lupus erythematosus. Lupus erythematous panniculitis typically presents with firm, indurated subcutaneous nodules or plaques commonly developed in proximal extremities, trunks, head and neck. Face, breast and upper extremities involvement with lupus erythematous panniculitis has been infrequently reported. We report a case of lupus erythematous panniculitis developed in a 27-year-old female confirmed by histologic features and laboratory findings.

기체 방사성 물질 제거를 위한 첨착 활성탄 필터 시스템 성능에 관한 연구

김대욱,손현철,이명석,이석범,박길문 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.1

This paper presents an application of impregnated activated carbon and impregnated activated carbon filters. They are used for the removal of gaseous radioactive materials at radioactive material handling facilities such as nuclear power plants and research facilities. High adsorption efficiencies as well as stability are required for them to remove hazardous radioactive gases such as activated free iodine released during normal operation or accident condition. This research was initiated to enhance the independence of nuclear industry and raise the level of nuclear technology to that of the leading nuclear nations. By attaining the capability of design and development of HVAC system, national technological competitiveness can be achieved. Also, the ground for technology export will be hardened. Therefore, it is well timed to initiate the project to develope those filter systems domestically utilizing all the relevant technologies available.

인터넷 기업가치평가에 관한 연구

이균봉,손명현 釜山大學校 商科大學 2002 釜山商大論集 Vol.73 No.-

This study is to valuate the internet corporate listed on KOSDAQ. The traditional valuation method based on only historical accounting incomes is not fully adequate for evaluation of internet corporate. Though the initial growth rates of internet companies are very high in their early stages, their operating incomes are very low because of high marketing and investment expenditures. This study was conducted on the samples of 42 firms that have reported their ratios of sales changes in individual company level for 3 years listed on KOSDAQ in 2000. Black-Scholes' option pricing model and DCF model were used to test the difference between the estimated corporate value in this study and real stock price. The result of study is as follows : There is no difference between estimated stock price by option model and real stock price. The result shows the stock price estimated by DCF model didn't explain real stock price. The sensitivity analysis by variables in both model indicates that DCF model is more sensitive than option model. DCF model consist of 4 estimated variables, while option model has only one. It can be interpreted that DCF model produces bigger variable measurement errors compared to option model. But option model is more appropriate to value the internet company's stock price than DCF model.

주의력결핍 과잉행동장애 아동에서 틱 장애 동반 여부에 따른 확산 텐서 영상 비교 연구

최지욱,임명호,이창화,박진균,손정우,심세훈,유인규,강현수,정범석 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : Diffuse tensor imaging (DTI) was applied to explore the difference in regional distribution and extent of white matter (WM) abnormalities in boys with Attention-deficit/hyperactivity disorder (ADHD) versus boys with comorbid ADHD and tic disorders. Methods : Fifteen boys with ADHD (mean age 9.3 +1.8), 24 ADHD boys with chronic tic disorder or Tourette's disorder(9.9 =b 1.2) and 9 age-, gender-matched controls (9.2 d= 1.8) received DTI assessments. Fractional Anisotropy (FA) maps of WM were compared between groups with a voxel-wise analysis after intersubject registration to MNI space. Results : Bo groups, ADHD group and ADHD with tic disorder group, commonly showed decreased FA than healthy control group in left cerebellar middle peduncle and right frontal lobe, increased FA in right middle occipital WM. In the common areas of left cerebellar middle peduncle and right middle occipital WM, comorbid group showed broader areas of significant FA. The comorbid group also showed increased FA in right cerebellar peduncle, additionally. Conclusion : The findings in ADHD group support previous ADHD hypothesis of the functional abnormalities in corticocerebellar circuit, and suggest that ADHD might have more complicated pathology of neuronal circuit including occipital visual system. The comorbid group showed common areas of overlapping but more extensive abnormalities and also had additional WM abnormalities. ADHD with chronic tic disorders may represent a severe form of ADHD with additional regions of abnormal connectivity.