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Construction of naphthalene diimide-based DNA nanowires and their application for DNA sensing
Shigeori Takenaka 대한전자공학회 2009 ITC-CSCC :International Technical Conference on Ci Vol.2009 No.7
Here I discuss the functionalized naphthalene diimide derivatives which can be used as a new coating reagent for DNA duplexes to yield a DNA-templated conductive nanowire. Since the functionalized naphthalene diimdes are concentrated on the DNA duplex by threading intercalation where the naphthalene diimides are arranged on the DNA duplex every two base pairs under saturated conditions, the DNA duplex was coated by their functionalized molecules. As an example of this system, many ferrocene molecules were arranged on the DNA duplex after treatment with ferrocenylnaphthalene diimde (FND). The pseudo-polyferrocene array template to DNA duplex thus obtained was expected to serve as a new nanowire for nanotechnology including electrochemical gene detection. Since the pseudo-polyferrocene array formed on the electrode is analyzed electrochemically, the target DNA could be detected by hybridization with a DNA probe-immobilized electrode and by the FND binding. The author’s group established an FND-based hybridization assay and extended to an electrochemical DNA chip system together with the analysis of single nucleotide polymorphisms (SNPs). Finally, the author’s group succeeded in the construction of DNA-templated silver nanowire using naphthalene diimide having galactose units (GND).
DNA Ligand - Redox Active Molecule Conjugates as an Electrochemical DNA Probe
Ihara, Toshihiro,Maruo, Voshiyuki,Uto, Yoshihiro,Takenaka, Shigeori,Takagi, Makoto 한국분석과학회 1995 분석과학 Vol.8 No.4
Toward the development of universal, sensitive, and convenient method of DNA (or RNA) detection, two kinds of electrochemically active DNA ligands. acridine - viologen and oligonucleotide - ferrocene conjugate, were prepared. Thermodynamic and electrochemical study revealed that these probes bound strongly to DNA, and showed a typical cyclic voltammograms, indicating a potential for use as a reversible electrochemical labelling agent for DNA. Especially, using the electrochemically active oligonucleotide, we have been able to demonstrate the detection of DNA at femtomole levels by HPLC equipped with ordinary electrochemical detector (ECD). These results lead to the conclusion that the redox-active probes are very useful for the microanalysis of nucleic acid due to the stabilily of the complexes, high detection sensitivity, and wide applicability to the target structures (single- and double strands) and sequences.