버섯으로부터 새로운 암전이 억제물질 개발을 위한 혈관신생 억제물질의 탐색

정승찬 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

버섯으로부터 새로운 암전이 억제물질을 개발하기 위하여 먼저 7종 52균주의 자실체 (또는 균사체)에 대한 다양한 추출물들을 제조한 후 이들의 암전이 억제에 관련된 혈관신생 억제활성을 수정란을 이용하여 Choriollantoic membrane(CAM) assay로 조사하였다. 7종 52균주의 버섯에 대한 물, 에탄올, 메탄올 추출물 중에서 장수버섯(Fomitella fraxinea) ASI 17003과 17009의 물 추출물과 비늘버섯(Pholiota sp.) ASI 24008과 잎새버섯(Grifola frondosa) ASI 9017의 에탄올 추출물, 그리고 차가버섯(Inonotus obliquus) ASI 74012의 메탄올 추출물에서 10㎍/egg농도일 때 62.5%~68.8%의 높은 혈관신생 억제활성을 보였다. 그러나 추출 수율과 안전성 등을 고려하여 비늘버섯 ASI 24008을 혈관신생 억제효과 우수버섯으로 최종 선정하여 현재 이들의 암전이 억제 기작 규명을 위한 정제 실험을 실시하고 있다. This study was carried out to obtain new cancer metastasis inhibitor from mushrooms. Extracts from 52 isolates belong to 7 species of mushrooms were prepared by water, ethanol and methanol extractions and its antiangiogenic activity were investigated by choriollantoic membrane(CAM) assay. Water extracts of Fomitella fraxinea ASI 17003 and ASI 17009 fruiting bodies, ethanol extract of Pholiota sp. ASI 24008 and Grifola frondosa ASI 9017 fruiting bodies and methanol extract of Inonotus obliquus ASI 74012 mycelia had the potential anti-angiogenic activity of 62.5%~68.8%. Finally, Pholiota sp. ASI 24008 was selected as a producer of cancer metastasis inhibitor on the basis of their solid yield and anti-angiogenic activity, etc.

Expansin으로 형질전환 된 담배의 생장 특성

신하정, 성현정, 백승준, 김보미, 엄유리, 조은정, 정찬문, 이이 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

-본문참고-

정신분열병 또는 정신분열형장애를 가진 입원환자에서 Risperidone과 Olanzapine의 효과의 비교 : 6주간의 개방적 전향적 연구 6 Weeks, Open-Labelled, Prospective Study

김찬우,이동건,강승훈,조지현,이수진,조동환,김정기 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2

Objectives : Risperidone and olanzapine, which are atypical antipsychotics. are widely used in the treatment of schizophrenia as the 1st line drug, proving their effectiveness for both positive and negative symptoms. They also have many advantages even from the side effects. However, they show differences with the chemical structure and pharmacokinetics and they work differently to receptors. Researchers have studied the effect and side effect during the acute phase of schizophrenia as an open prospective study. Methods : Forty subjects with DSM-IV schizophrenia or schizoaffective patients including 1) 1st onset schizophrenia patient, 2) patient who has been treated by the prior antipsychotics and not improved or even worse to be admitted again, were assigned to receive risperidone or olanzapine for 6 weeks. Psychopathology, adverse effect. weight change, fasting serum glucose, and drug cost were compared within and across groups. Results : After 6 weeks later both treatment groups showed the significant decrease in PANSS positive, general psychopathology and total score while they did not show any significant difference between two groups. Risperidone group, however. showed the significant decrease in PANS negative score, which olanzapine group did not. In terms of adverse effect risperidone group showed more extrapyramidal symptom than olanzapine group. Body weight rised significantly after Gweeks, but no significant difference between two treatment groups. There were no significant indrease of fasting serum glucose level. Conclusions : Risperidone and olanzapine are effective drugs which can be used during the acute phase of schizophrenia. Risperidone is expected to shorten the admission day and remission of the symptom of acute phase, mile olarizapine is more advantage in a neurologic side effect than rispendone.

분편 인플루엔자백신(split influenza vaccine)의 임상효과 및 면역원성에 관한 연구

우흥정,김동림,정희진,천병철,이주연,안정배,김지희,박찬,신영규,김우주,김민자,박승철 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 아단위 인플루엔자 백신 접종 후 백신의 인플루엔자 예방효과, 인플루엔자 방어 항체형성, 인플루엔자 백신의 안전성을 조사하고자 하였다. 방법 : 총 571명을 대상으로 인플루엔자 백신 접종을 하였고, 이들 접종자에서 인플루엔자 양질환의 이환을 조사하여 인플루엔자 백신의 인플루엔자예방효과를 알아보았고, 백신의 접종 전 및 접종 4주 후 혈청에서 혈구응집억제물(Hemagglutination Inhibition : HAI) 항체 검사를 실시하여 백신의 방어항체생성을 조사하였고, 백신의 안전성을 알아보기 위해 백신접종 후 1주일 이내의 부작용을 조사하였다. 결과 :백신 접종군과 백신 비접종군에서 인플루엔자 양 질환의 이환을 조사한 결과 접종군 28.35%, 비접종군 35.88%으로 나왔으며, p 값이 0.001로 통계적으로 접종군에서 유의하게 낮았고, 인플루엔자 양 질환의 예방 효과는 20.97%를 보였다. 백신의 방어항체 형성의 평가를 위해 유럽의 인플루엔자 백신 허가 기준을 조사하였는데 B/Guangdong/5/94균주의 백신접종 후 항체가 40이상의 비율을 제외한 다른 기준은 모두 만족 시켰다. 부작용은 전체 조사자 521명중 149명(29%)으로 주로 접종 부위의 국소 부작용을 호소했고, 전신 부작용은 2% 내외였으며 특별히 심각한 부작용은 발견되지 않았다. 결론 : 분편 인플루엔자 백신은 인플루엔자양질환의 예방과 방어항체생성에 효과 있으며 안전한 것으로 사료된다. Background : The safety and effectiveness of influenza vaccine are well known in developed country. The influenza vaccination has been recommended as one of the tentative immunization schedule for indicated persons since 1997 in Korea. But there are still no available data about them, even though nearly 5 million doses of influenza vaccine were used in 1997-1998 season. So it is immediately needed to investigate the safety. efficacy and immunogenicity of influenza vaccine among Korean. Methods : We studied the clinical efficacy of influenza vaccine by monitoring Occurrence of influenza-like illness in influenza risk group(vaccination ; 300, non-vaccination; 215) from December in 1997 to March in 1998. We used the split quadrivalent influenza vaccine containing 15 microgram of hemagglutinin of A/Beijing/262/95(HlNl), A/Wuhan/359/95(H3N2), B/Mie/1/93 and B/Guangdong/5/94. Hemagglutination inhibition(HA1) antibody titers were determined before immunization and 1 months after vaccination And we evaluated adverse effect of influenza vaccination at 7 days after vaccination. Results : Influenza vaccination was associated with si@icant reductions in influenza-like spptoms(vaccination group; 28.35%, non-vaccination group, 35.88%, p=0.001). The preventive effect of influenza-like i3lne.s among influenza risk goup was 20.97%. And immunogenicity of influenza A and B exceeded all of the European licensure criteria for immunogenicity except postvaccination proportion of titers 240 of B/Guangdong/5/94 strain. And the adverse effects were mainly local injection site problem and no serious adverse effect was noted. Conclusion : Split influenza vaccine is safe, inmunogenic and eff'tive in influenza risk group in Korea.

컨테이너 운송용 AGV의 운동궤적에 관한 연구

박정보,김민주,이승수,김중완,전언찬 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.5

In this study, we have developed the simulation tool in order to investigate driving trajectory of AGV for container transport. AGV for container transport is different from the indoor AGV in that it is a large size structure at being loaded the weight of 40 ton. And AGV for container transport is applied to front wheel steering, rear wheel steering, all wheel steering, and crap steering. Therefore, we have developed the simulation tool considering dynamic problems and a center of turning in accordance with fourth ways of steering mode. As the result of this study, we have confirmed that this tool is useful and cost-effective in the dynamic analysis or large size vehicles. Also, it is useful to calculate the minimum radius of turning for large size vehicles.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

갈륨액체금속 이온원과 인듐액체금속 이온원의 빔 특성에 대한 연구

현정우,임연찬,정강원,정원희,박철우,이종향,강승언 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

본 연구에서는 인듐 액체금속이온원을 제작하여 빔 특성에 대해 연구를 하였으며, 기존에 연구를 하였던 갈륨 액체금속이온원의 빔특성과 비교 분석 하였다. 빔특성 분석을 위해 빔 안정도, 전류-전압특성곡선, 에너지 퍼짐을 측정하였다. 액체금속이온원에 사용되는 액체금속 저장소 및 바늘전극(tip)은 500μm의 직경을 갖는 텅스텐을 사용하였으며, 국내에서 제작된 제품을 사용하였다. 액체금속 저장소의 구조는 이전에 구상하여 연구가 이루어진 6개의 pre-etching된 텅스텐와이어(wire)가 묶여진 형태를 사용하였다.

뇌경색후 발생한 Cortical Spreading Depression과 뇌경색 범위에 관한 실험적 연구

박윤관,이승민,김세훈,이기찬,주정화,이훈갑 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.3

Cortical Spreading Depression(CSD) is a transient depression of neuronal activity that spreads across the cortical surface and is associated with profound changes in blood flow, extracellular ion concentration. Direct Current(DC) potentials and cell membrane potentials. One of the electrophysiological disturbance in the periinfarct surrounding is spontaneous occurrence of repeated CSD like DC shifts associated with increased energy demand. Due to restricted blood flow to the periinfarct border zone, elevated metabolic demand is potentially harzardous. So the authors designed this experiment to verify the correlation between periinfarct cortical spreading depression and ischemic volume following permanent middle cerebral artery (MCA) occlusion in rats. Sprague-Dawley rats(n=27) were anesthetized with 0.5∼1% halothane, and artificially ventilated through a tracheal cannula. Arterial pressure, blood gases and body temperature were controlled. The middle cerebral artery(MCA) was occluded distally to the lenticulostriate branches. Measurements of CSD activity were made for 4 hours in each animal. Infarct volume was determined 6 hours later in 2, 3, 5-triphenyl tetrazolium chloride(TTC)-stained sections. For 4 hours after MCA occlusion, the CSDs were found in all experimental animals with a range of 2∼9 times. Those CSDs wer of varying duration, : "small"(<1 min) and "big"(> 1 min) SDs and mean of total duration of SD was 10.5±10.3 min during 4 hours of MCA occlusion. Neuropathological evaluation of brain infarct in the rats, which had been allowed to survive for 6 hours after MCA occlusion showed a mean volume of 89.7±45.3㎣. Serial observation of duration of CSD showed progressive prolongation only in 8 rats. The rest of them(n=19) did not show any prolongation. Neither the prolongation of duration of CSD nor the frequency of CSD in the penumbral zone correlated with the volume of infarct. However total duration of CSD was slightly related with the infarct volume after 6 hours of the permanent MCA occlusion(r=0.414, p=0.0318).

컴퓨터 작업 시 의자 등받이 위치가 근육 활성도에 미치는 영향

김민우,변승진,이경은,정소영,조주영,최원자,최찬양,김경 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 컴퓨터 작업시 의자 등받이 위치가 근육활성도에 미치는 영향을 알아보기 위해 36명의 근골격에 문제가 없는 성인 남녀가 참가하였으며 대상자들은 등받이가 뒤에 있는 의자와 등받이가 없는 의자, 등받이가 앞에 있는 우리들 의자를 무작위로 배정하고 동일한 컴퓨터 작업을 실시하게 하였다. 실험 중, 표면근전도기를 사용하여 의자에 따른 위등세모근, 머리널판근 그리고 척주세움근의 근활성도 변화를 측정하였다. 이 연구의 결과를 종합해보면 등받이가 앞에 있는 의자는 허리 근육의 부담을 줄이지만 목근육에 부담이 늘어나고 등받이가 뒤에 있는 의자와 없는 의자는 허리근육에는 다소 무리가 가지만 목근육에는 부담이 덜하다는 것을 알 수 있었다. 이러한 결과로 환자의 증상에 다라 컴퓨터 작업을 하는 동안 의자 등받이의 위치를 조절하여 치료에 도움이 될 수 있다고 생각되어진다. Objective : In this study, we checked and observed people's change of body muscle and muscle activity who do computer work on different chairs. We set three kind of chairs which one is a stool and another one is the back is fixed, and the other one is the back is fixed in front of chair. Subject : Accounting for 36 health men and women, we divided these people to 3 groups and put them on the three cases(position 1, position 2, position 3). We measured muscle activity of upper trapezius, Splenius Cervics, Erector Spinae from the groups by using surface electromyogram system. Methods : The standard of the electromyogram was 'reference voluntary contraction', and we carried out 'one way anova' to compare muscle activity of three groups. Results : In position 1, reference voluntary contraction of upper trapezius muscle activity was 123.2821%, Splenius Cervics was 141.7526%, and Erector Spinae was 254.5233%. In position 2, reference voluntary contraction of upper trapezius muscle activity was 132.9395%, Splenius Cervics was 141.7526%, and Erector Spinae was 246.6540%. In position 3, reference voluntary contraction of upper trapezius muscle activity was 190.6487%, Splenius Cervics was 270.2332%, and Erector Spinae was 182.1021%. Both upper trapezius and Splenius Cervics muscle activity of position 3 group was higher than the others groups. In position 1 group, Erector Spinae muscle activity was hight than the other groups. And either was position 2 group's(p<0.05). Conclusion : Therefore the chair which is the back is fixed in front is more comfortable for waist muscle but it's not good for neck. And the other chairs are better in waist muscle but not in neck muscle. So far, when we do a computer work, it's prefer to sit on the group 1&2's chairs to reduce neck muscle's stress and sit on group 3's chair to reduce waist muscle's stress.

광주전남지역 정상 신생아의 코의 형태와 계측치에 대한 연구

이유진,양정열,이승찬,천지선,박상기 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

Background and Objectives: Cleft lip and palate are common defects in congenital deformity. They are the most frequent deformity in the face. Nose deformity is most common among various facial deformity at the center of face. So we must operate to correct the deformity at the earliest stage to give a patient and parent spiritual relief from anxiety, and it is the trend of the treatment recently. We must know the facial anthropometry in newborn to try at the early stage, but there is a lack of literature and research on newborns. So we carried out this research to form base data for operations. Materials and Methods : The noses of 90 newborns in Gwangju-Cheonnam area were analyzed quantitatively, based on 10 nasal measuring points and 13 anthropometric values taken directly from the face of newborns. The relationship between the nasal measurements were studied in 11 proportion indices. Results : Following are our mean values in newborn. Width of the nasal root was 13. 9mm. Width of the nose was 22. lrnm, Width of the columella was 4. 5mm. Height of the nose was 22.6mm. Length of the nasal bridge was 20.1mm. Nasal tip protrusion was 8.9mm. Width of the nostril floor was 6.4mm. Thickness of the nasal ala and length of the nasal ala were 4.0mm, 15.4mm. Length of the columella was 3.9mm. The most common nostril shape was Type II (0-45 degrees in inclination of the nostril axis from the horizontal). Nasal index was 97.8. Columella-nasal tip protrusion length index was 43.9. Conclusion : The development of the columella was relatively incomplete in the case of newborn and was the result to appear. The normal indicies could be used clinically in identifying the it's normality as well as morphologic characteristics of the craniofacial structures. They also could be used in differential diagnosis between the morphologically similar congenital craniofacial anomalies. In preoperative design, in setting the criteria of the postoperative results, and assessing the effect of the operation, Finally, they supply the plastic surgeon and the other specialists in the craniofacial surgery with basic normal population data.