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Modification of Beef Tallow by Supercritical Carbon Dioxide
Lim,Sang-Bin,Rizvi, Syed S.H 제주대학교 1993 논문집 Vol.36 No.-
48℃, 276 bar의 초임계 이산화탄소를 이용하여 식용 쇠고기기름을 가공하였다. 쇠고기기름의 추출율은 시간당 11%였다. 총 콜레스테롤 제거율은 75%였으며, 이 때 지방의 회수율은 86%였다. 쇠고기기름을 물리화학적 성질이 다른 분획으로 분별하였다. 중급트리글리세리드(C44-C48)의 농도는 추출된 분획들에서 높았으며, 잔류물에는 고급트리글리세리드(C50-C54)가 다량 함유되어 있었다. 추출물에는 저급지방산의 함유율이 높았다. 잔류물에는 스테아르산의 함량이 특히 높았으며, 이로 인하여 높은 녹는점을 보였다. 고형지방은 여러 온도에서 추출분획들 보다 잔류물에 많았다. Edible beef tallow was processed using supercritical carbon dioxide at temperature of 48℃ and pressure of 276 bar. The extraction efficiency of the process was 11% per hour. The total cholesterol reduction was 75% with 86% fat yield. A simultaneous fractionation of beef tallow with different physico-chemical properties was also achieved. The concentration of medium (C44-C48) molecular weight triglycerides was high in the extracted fractions. The residue was enriched in high (C50-C54) molecular weight triglycerides. The extracts contained more fatty acids with shorter carbon chains. The residue had the highest melting points, mainly caused by having higher stearic acid content. The extracts showed lower solid fat index than the residue at several temperatures.
Lim, Ji-Young,Ryu, Da-Bin,Park, Mi-Young,Lee, Sung-Eun,Park, Gyeongsin,Kim, Tai-Gyu,Min, Chang-Ki Elsevier 2018 BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION Vol.24 No.12
<P><B>Abstract</B></P> <P>Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells with anti-inflammatory activity, and expanded murine MDSCs are capable of attenuating preclinical acute graft-versus-host disease (aGVHD) severity. Two murine cGVHD models were used to evaluate the effectiveness of ex vivo cultured human cord blood (hCB) MDSCs in chronic GVHD (cGVHD). First, GVHD recipients surviving in a classic C57BL/6 into MHC-mismatched BALB/c aGVHD model developed cGVHD. Second, donor pretreatment with granulocyte colony-stimulating factor (G-CSF) induced cGVHD. hCB-MDSCs (1 × 10<SUP>6</SUP>) were intravenously injected to determine their preventive effects (on days 5, 7, 10, and 21) or therapeutic effects (on days 21, 28, and 35). In the first model the onset of clinical cutaneous cGVHD was significantly delayed in preventive hCB-MDSCs–treated allogeneic recipients. Pathologic scoring of target organs confirmed these clinical results. Importantly, thymic tissues of GVHD mice treated with hCB-MDSCs were less severely damaged, showing higher numbers of double (CD4 and CD8) positive T cells with reduced expansion of donor-type CD4 and CD8 T cells. Moreover, late infusion of hCB-MDSCs controlled the severity of established cGVHD that had occurred in control recipients. In the second model, cGVHD induced by G-CSF–mobilized stem cell graft was associated with promotion of Th 17 and Th 2 differentiation. hCB-MDSCs attenuated clinical and pathologic cGVHD severity. Increased production of IL-17 and more infiltration of T cells and macrophages in cGVHD mice were markedly reduced after hCB-MDSCs treatment. Importantly, Foxp3<SUP>+</SUP> regulatory T cells and IFN-γ–producing T cells were expanded, whereas IL-17– and IL-4–producing T cells were decreased in allogeneic recipients of hCB-MDSCs. Taken together, these results showed that hCB-MDSCs have preclinical capability of attenuating cGVHD by preserving thymus function and regulating Th 17 signaling, suggesting a possible therapeutic strategy for clinical application.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Human cord blood (hCB) can be a source of immunosuppressive MDSCs. </LI> <LI> Administration of hCB-MDSCs attenuates cGVHD in preclinical models. </LI> <LI> hCB-MDSCs reduce thymic damage with less donor-derived T cell expansion. </LI> <LI> cGVHD protection by hCB-MDSCs correlate with reversal of Th 17 skewing. </LI> <LI> hCB-MDSCs modulate helper T cell subset and expand FoxP3 Treg. </LI> </UL> </P>
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
Sung-Bin Lim,Seok Ho Cha,Seung Jegal,Hojong Jun,Seo Hye Park,Bo-Young Jeon,Jhang Ho Pak,Young Yil Bakh,Tong-Soo Kim,Hyeong-Woo Lee 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.4
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3-O-P bond of RNA in a DNA-RNA duplex, producing 3-hydroxyl and 5-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
Sang-a Park,Gihwan Byeon,Jin Hyeong Jhoo,Hyung-Chun Kim,Myoung-Nam Lim,Jae-Won Jang,Jong Bin Bae,Ji Won Han,Tae Hui Kim,Kyung Phil Kwak,Bong Jo Kim,Shin Gyeom Kim,Jeong Lan Kim,Seok Woo Moon,Joon Hyuk 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.4
Objective: To investigate the relationship between reduced glutathione (GSH), a key molecule of the antioxidant defense system in the blood, and glutathione reductase (GR), which reduces oxidized glutathione (glutathione disulfide [GSSG]) to GSH and maintains the redox balance, with the prevalence of Alzheimer’s dementia and cognitive decline. Methods: In all, 20 participants with Alzheimer’s dementia who completed the third follow-up clinical evaluation over 6 years were selected, and 20 participants with normal cognition were selected after age and sex matching. The GSH and GR concentrations were the independent variables. Clinical diagnosis and neurocognitive test scores were the dependent variables indicating cognitive status. Results: The higher the level of GR, the greater the possibility of having normal cognition than of developing Alzheimer’s dementia. Additionally, the higher the level of GR, the higher the neurocognitive test scores. However, this association was not significant for GSH. After 6 years, the conversion rate from normal cognition to cognitive impairment was significantly higher in the lower 50th percentile of the GR group than in the upper 50th percentile. Conclusion: The higher the GR, the lower the prevalence of Alzheimer’s dementia and incidence of cognitive impairment and the higher the cognitive test scores. Therefore, GR is a potential protective biomarker against Alzheimer’s dementia and cognitive decline.
Design Consideration of Back-EMF Constant for 3-D.O.F. Spherical PM Motor
Sung-chul Go,Dong-woo Kang,Jong-bin Im,Ju Lee,Sung-hong Won,Seung-Bin Lim 한국자기학회 2010 Journal of Magnetics Vol.15 No.2
A 3-D.O.F. spherical PM motor has 3 degrees of freedom in its motion by tilting and rotating of a shaft, which can be applied in a range of fields. The back-EMF is proportional to the field flux and angular velocity. The back-EMF constant in conventional rotating machine has a uniform value. However, in a spherical PM motor, the back-EMF constant of the coils varies according to the tilting conditions regardless of whether the angular speed is constant. Consideration of the back-EMF constant is useful for designing 3-D.O.F. spherical PM motors. In this study, the back-EMF constant of the spherical PM motor was considered carefully.
Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast
Sung, Min-Kyung,Lim, Gyubum,Yi, Dae-Gwan,Chang, Yeon Ji,Yang, Eun Bin,Lee, KiYoung,Huh, Won-Ki Cold Spring Harbor Laboratory Press 2013 Genome research Vol.23 No.4
<P>The definition of protein–protein interactions (PPIs) in the natural cellular context is essential for properly understanding various biological processes. So far, however, most large-scale PPI analyses have not been performed in the natural cellular context. Here, we describe the construction of a <I>Saccharomyces cerevisiae</I> fusion library in which each endogenous gene is C-terminally tagged with the N-terminal fragment of Venus (VN) for a genome-wide bimolecular fluorescence complementation assay, a powerful technique for identifying PPIs in living cells. We illustrate the utility of the VN fusion library by systematically analyzing the interactome of the small ubiquitin-related modifier (SUMO) and provide previously unavailable information on the subcellular localization, types, and protease dependence of SUMO interactions. Our data set is highly complementary to the existing data sets and represents a useful resource for expanding the understanding of the physiological roles of SUMO. In addition, the VN fusion library provides a useful research tool that makes it feasible to systematically analyze PPIs in the natural cellular context.</P>
Prognostic factors associated with the success rates of posterior orthodontic miniscrew implants
Sung-Bin Hong,Budi Kusnoto,Eun-Jeong Kim,Ellen A BeGole,Hyeon-Shik Hwang,Hoi-Jeong Lim 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.2
Objective: To systematically review previous studies and to assess, via a subgroup meta-analysis, the combined odds ratio (OR) of prognostic factors affecting the success of miniscrew implants (MIs) inserted into the buccal posterior region. Methods: Three electronic searches that were limited to articles on clinical human studies using MIs that were published in English prior to March 2015 were conducted. The outcome measure was the success of MIs. Patient factors included age, sex, and jaw of insertion (maxilla vs. mandible), while the MI factors included length and diameter. A meta-analysis was performed on 17 individual studies. The quality of each study was assessed for non-randomized studies and quantified using the Newcastle-Ottawa Scale. The meta-analysis outcome was a combined OR. Subgroup and sensitivity analyses based on the study design, study quality, and sample size of miniscrews implanted were performed. Results: Significantly higher success rates were revealed for MIs inserted in the maxilla, for patients ≥ 20 years of age, and for long MIs (≥ 8 mm) and MIs with a large diameter (> 1.4 mm). All subgroups acquired homogeneity, and the combined OR of the prospective studies (OR, 3.67; 95% confidence interval [CI], 2.10−6.44) was significantly higher in the maxilla than that in the retrospective studies (OR, 2.10; 95% CI, 1.60−2.74). Conclusions: When a treatment plan is made, these risk factors, i.e. jaw of insertion, age, MI length, and MI diameter, should be taken into account, while sex is not critical to the success of MIs.
Protein Kinase $C\alpha$ Is Involved in the Cell Condensation During Chondrogenesis in Vitro
Lim, Young-Bin,Kang, Shin-Sung,Lee, young-Sup,Sonn, Jong-Kyung The Korean Society for Integrative Biology 2000 Korean journal of biological sciences Vol.4 No.4
In order to investigate the role of protein kinase C (PKC) in chondrogenic differentiation, we examined the localization of PKC isoforms in a limb bud micromass culture system. PKC$\alpha$ is specifically localized in the regions which would become cartilage nodules, while PKC$\lambda/l$ and $\zeta$ display widespread distribution in the whole culture. Distribution of PKC$\alpha$ change along with promotion or inhibition of chondrogenesis by lysophosphatidylcholine or phorbol 12-myristate 13-acetate. On the other hand, localization of PKC$\lambda/l$ or $\zeta$ a was not changed by the modulation of chondrogenesis. Peanut agglutinin binding protein which is associated with cell aggregation during chondrogenesis was present in the cell condensation regions and its expression in those regions was influenced by PKC activity. Expression of fibronectin and N-cadherin in the cell condensing area were also affected by modulation of PKC activity. These results suggest involvement of PKC$\alpha$ in the cell condensation, possibly through regulating expression of fibronectin and N-cadherin.