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이새미,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1
A 11-year-old Yorkshire terrier anesthetized for computed tomography. The dog showed cyanosis and syncope during postanesthetic recovery, therefore drug administrated parenterally. Goose-honking was noticed after full recovery. As a result of survey radiography and fluoroscopy, grade 4 tracheal collapse was diagnosed. Prescription for the dog was oral administration of hydrocodone 0.22 mg/kg. theophylline 20 mg/kg, prednisone 0.5 mg/kg, amoxicillin 22 mg/kg and chlorpheniramine 2 mg/kg twice a day for 3 days. A 10-year-old Pomeranian was diagnosed as grade 2 traceal collapse at the local animal hospital 2 years before being referred to the veterinary teaching hospital of Chungnam National University. On physical examination, goose-honking, coughing and intermittent open-mouth breathing. Tracheal narrowing was observed on survey einradiography and fluoroscopy. Oral administration of theophylline 13 mg/kg, enrofloxacin 5 mg/kg, chlropheniramine 0.5 mg/kg twice a day was prescribed for 2 weeks.
Lee, Sae-Mi,Lee, Mi-Na,Oh, Hyeon Ju,Cho, Yoon Young,Kim, Jae Hyeon,Woo, Hye In,Park, Hyung-Doo,Lee, Soo-Youn The Korean Society for Laboratory Medicine 2015 Annals of Laboratory Medicine Vol.35 No.5
<P>High-resolution imaging techniques have increased the detection rate of adrenal incidentaloma. We developed a method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detection of plasma free metanephrine (MN) and normetanephrine (NMN) and evaluated its analytical performance and clinical efficacy in differential diagnosis of adrenal incidentaloma. After solid-phase extraction, chromatographic isolation of the analytes and internal standard was achieved by column elution in the LC-MS/MS system. The analytes were detected in multiple-reaction monitoring mode by using positive electrospray ionization: MN, transition m/z 180.1→165.1; NMN, m/z 166.1→134.1. This method was validated for linearity, precision, accuracy, lower limits of quantification and detection, extraction recovery, and the matrix effect. Plasma concentrations of MN and NMN of 14 patients with pheochromocytoma were compared with those of 17 healthy volunteers, 10 patients with essential hypertension, and 60 patients with adrenal adenoma. The assay's linear range was 0.04-50.0 and 0.08-100.0 nmol/L for MN and NMN, respectively. Assay imprecision was 1.86-7.50%. The accuracy ranged from -7.50% to 2.00%, and the mean recovery of MN and NMN was within the range 71.5-95.2%. Our LC-MS/MS method is rapid, accurate, and reliable and useful for differential diagnosis of adrenal incidentaloma.</P>
Analysis of Clinical Characteristics and HLA types of Acute Generalized Exanthematous Pustulosis (초)
( Mi-Yeong Kim ),( Sung-Yoon Kang ),( Seo-Young Lee ),( Min-Hye Kim ),( Woo-Jung Song ),( Jae-Woo Kwon ),( Jae-Woo Jung ),( Tae-Whan Kim ),( Sae-Hoon Kim ),( Hye-Ryun Kang ),( Heung-Woo Park ),( Sun-S 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-
Sae Mi Lee,Won Jong Jang,Sung Min Kim,Yoon Hee Lee,Hyun Dong Shin,Yong-Il Park 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
The crushed fruiting body of Alaskan Phellinus pini (Brot.) Murrillwas extracted in boiling water for 4 h with the yield of 20.5% in dry mass. From which the ethanol precipitate (EP) and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 14.2 % in dry mass, respectively. From the EP preparation, two major carbohydrate-positive fractions, named EP-AV1 and EP-AV2, were purified by mainly Sepharose CL-4B column. Both EP-AV1 and EP-AV2 inhibited the plaque formation by CVB3 on HeLa cells by 32% and 84%, respectively, at 1 mg/mL, with EC50 of over 1 mg/ml(EP-AV1) and 0.576 mg/ml (EP-AV2), and the antiviral activity of these two polysaccharides against HSV-1 in Vero cells, inhibited in a dose-dependent manner at much lower concentrations (1 to 50 μg/ml), with EC50 of 0.20 μg/ml (EP-AV1) and 0.21 μg/ml (EP-AV2), respectively. Also, EP-AV1 and EP-AV2 inhibited neuraminidase activity by 27.4% and 52.4% at 1.7 mg/ml, and 44.1% and 73% at 3.4 mg/ml, respectively. EP-AV1 was shown to be a heteropolysaccharide containing glucose as the main sugar residue with mole percentage of 53.4% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%). EP-AV2 was also a heteropolysaccharide with glucose as the main sugar (56.1%) and other minor sugars similar to that of EP-AV1 but relatively lower proportion of galactose (10.3%). The HPAEC-PAD analysis after laminarinase digestion showed that both polysaccharides contain β-1,3-linked glucose residues, suggesting that these polysaccharides are a β-(1,3)-glucan. The results of this study suggest that these water-soluble polysaccharides of Alaskan P. pini fruiting body can be a strong candidate for the development of a potent broad-range antiviral agent.