DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

푸른길 공원의 대기 환경 특성에 관한 연구

민경우,이경석,박옥현,윤관주,김도술,박세일,정원삼,이대행,조영관,Min, Kyoung-Woo,Lee, Kyoung-Soek,Park, Ok-Hyun,Yoon, Kwan-Ju,Kim, Do-Sool,Park, Se-Il,Jeung, Won-Sam,Lee, Dae-Hang,Cho, Young-Gwan 한국환경보건학회 2015 한국환경보건학회지 Vol.41 No.3

Objectives: This study aimed to survey the characteristics of air quality and meteorological conditions in a greenway park. Methods: We measured meteorological and health related factors, including noise, particulate matter ($PM_{10}$) and selected gaseous air pollutants at three locations in a greenway park and on a general roadside as comparison. The measurements were repeated four times from April to October 2014. Results: The average air temperature in the greenway park was $20.7^{\circ}C$ which was $1-2^{\circ}C$ lower than on the general roadside. The average $PM_{10}$ concentration in the greenway park was $85.0{\mu}g/m^3$, a level 2-3 times lower than that at the roadside. The noise level at the greenway site was 4.4 dB(A)- 23.0 dB(A) lower than at the roadside. The average CO, $CO_2$, $SO_2$ and NOx concentrations in the greenway park were lower than at the roadside. The average phytoncide and anions concentrations in the greenway park were higher than at the roadside. Conclusions: The urban forest of the greenway park may have some impact on air quality and meteorological conditions.

알레르기 환자에서 한방 이외의 대체 요법의 사용 현황에 대한 다기관 조사

이현정 ( Hyun Jung Lee ),이재현 ( Jae Hyun Lee ),이용원 ( Yong Won Lee ),김철우 ( Cheol Woo Kim ),동헌종 ( Hun Jong Dhong ),박해심 ( Hae Sim Park ),조영주 ( Young Joo Cho ),조진희 ( Jin Hee Cho ),조상헌 ( Sang Heon Cho ),편복양 ( 대한내과학회 2011 대한내과학회지 Vol.80 No.1

Background/Aims: The use of unproven complementary/alternative medicine (CAM) to treat allergies is popular in Korea. We conducted a multicenter survey of the current use of CAM other than herbal medication in Korean allergy patients. Methods: This study enrolled 510 adults with allergic diseases, including asthma, allergic rhinitis, atopic dermatitis, and chronic urticaria, from ten hospitals. They underwent a structured questionnaire interview and clinical assessment of the prevalence, motivation, costs, and subjective assessment of CAM. Results: Of the patients, 16.7% used at least one type of CAM to treat their allergic diseases. Common types of CAM were diet (48.6%), aroma therapy (23.8%), massage (14.3%), breathing exercises (7.6%), baths (5.7%), and mind control (5.7%). The therapeutic effects of CAM included improved in patients using mind control (50%), diet (50%), breathing exercises (75%), and baths (33.3%), but worse in patients using aroma therapy (28%), and massage (26.7%). About 36.4% of the patients used CAM more than four times per year, and the average cost per CAM user was 370,000 won/year. The main reason for trying CAM was `hope to improve my constitution`. Conclusions: CAM is used widely for treating allergic diseases in Korea. Detailed knowledge of CAM and patient education are important. Further studies of the clinical efficacy of CAM are needed. (Korean J Med 2011;80:68-77)

DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

경주국립공원의 관속식물상

윤정원 ( Jung Won Yoon ),김용식 ( Yong Shik Kim ),신현탁 ( Hyun Tak Shin ),김기송 ( Gi Song Kim ),성정원 ( Jung Won Sung ),이창현 ( Chang Hyeon Lee ),박기환 ( Ki Hwan Park ),이명훈 ( Myung Hoon Yi ) 한국환경생태학회 2013 한국환경생태학회지 Vol.27 No.2

본 연구는 경주국립공원(남산, 토함산, 단석산)의 관속식물상을 밝히고 주요 식물들의 분포를 조사하였다. 2011년 7월부터 2012년 5월까지 총 7회 조사하였다. 조사결과 관속식물은 96과 284속 385속 4아종 41변종 6품종으로 총 436분류군을 확인하였다. 그 중 산림청 지정 희귀식물은 7분류군, 특산식물은 13분류군이다. 식물구계학적 특정식물은 41분류군으로 Ⅴ등급 1분류군, Ⅳ등급 3분류군, Ⅲ등급 9분류군, Ⅱ등급 4분류군,Ⅰ등급 24분류군이다. 한편 귀화식물은 29분류군으로 확인하였으며, 귀화율은 6.65%, 도시화지수는 9.03%이다. 조사된 436분류군의 유용도는 목초용 자원식물이 182분류군(41.7%), 식용 176분류군(40.4%), 약용 147분류군(33.7%), 관상용 52분류군(11.9%), 목재용 18분류군(4.1%), 염료용 16분류군(3.7%), 섬유용 8분류군(1.8%), 공업용 자원식물이 1분류군(0.2%) 그리고 용도가 밝혀지지 않은 식물이 79분류군(18.1%)으로 나타났다. This study was carried out to investigate the vascular flora of Mt. Nam, Mt. Toham, and Mt. Danseok in Gyeongju National Park . The vascular plants were surveyed for 7 times from July 2011 to May 2012. The results were summarized as 436 taxa, 96families, 284 genera, 385 species, 4 subspecies, 41 varieties, and 6 forms. The 13 taxa were categorized as Korean endemic plants species and the other 7 taxa as rare plants species, which categorized by the Korea Forest Service. Based on the Floristic Degrees categorized by the Specific Distribution of Plants Species, one taxa grouped as the Floristic Degree Ⅴ, 3 for Floristic Degree Ⅳ, and 9 for Floristic Degree Ⅲ, 4 for Floristic Degree Ⅱ, and 24 taxa for Floristic Degree I. The naturalized plants were recorded as 29 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 6.65%, and 9.03%, respectively. 436 taxa listed consists of 182 taxa(41.7%) of pasturing plant, 176 taxa(40.4%) of edible plants, 147 taxa(33.7%) of medicinal plants, 52 taxa(11.9%) of ornamental plants, 18 taxa(4.1%) of timber plants, 16 taxa(3.7%) of stain plants, 8 taxa(1.8%) of fiber plants, 1 taxa(0.2%) of industrial plants and 79 taxa(18.1%) of unknown plants.

경주국립공원 문화재지정구역의 귀화식물 현황 및 관리방안

윤정원 ( Jung Won Yoon ),신현탁 ( Hyun Tak Shin ),이명훈 ( Myung Hoon Yi ),김기송 ( Gi Song Kim ),성정원 ( Jung Won Sung ),박기환 ( Ki Hwan Park ),이창현 ( Chang Hyun Lee ),김용식 ( Yong Shik Kim ) 한국환경생태학회 2012 한국환경생태학회지 Vol.26 No.4

경주국립공원 문화재지정구역에 분포하는 귀화식물은 총 14과 40속 45종 1변종 등 총 46분류군을 확인하였다. 귀화식물을 유형별로 구별해보면, 생활형은 1년생 초본이 17분류군(37%)으로 가장 많았으며, 다년생 초본이 16분류군 (34.8%), 2년생 초본이 11분류군(23.9%), 목본이 2분류군(4.3%)로 나타났다. 원산지 별로는 유럽이 17분류군(37%)으로 가장 많았으며, 그 다음으로 북아메리카에서 이입된 종이 16분류군(34.8%)으로 나타났다. 또한 귀화도는 3등급이 18분류군(40.9%)으로 가장 높다. 귀화식물 321분류군에 대한 본 연구대상지의 도시화 지수는 14.3%이며, 지구별 도시화 지수는 남산지구가 8.1%로 가장 높다. 경주국립공원은 사적형 공원으로 문화재가 매우 중요하나, 문화재 주변의식재 및 관리 또한 중요하다. 따라서 귀화식물의 경우 장기적인 모니터링과 생태적인 특성을 파악하여 체계적인 관리방안이 요구된다. The naturalized plant species in Gyeongju National Park was summarized as 46taxa including 14 families 40 genus 45 species 1 varieties. Among them, annual herbaceous species was 17taxa (37%) which is grouped as the biggest proportion and 16taxa (34.8%) for perennial herbaceous species and 11taxa (23.9%) for biannual herbaceous species and 2taxa (4.3%) for tree species. European origin was the biggest proportion as 17taxa (37%), and 16% (34.8%) from North America. The degree of naturalization (DoN) was the biggest for Degree III, V as 18taxa (39.1%). Regarding 321 naturalized plant species, the Urbanization Index in the studied area was 14.3%. The Namsam District showed 8.1% which is the highest DoN in the studied districts within the Gyeongju National Park. As the cultural properties is important in the Gyeongju National Park as a representatives for historical sites in Korea, planting and its management in the cultural properties is important tasks. The naturalized plant species in the studied area should be reasonably management.

심장혈관용 약물코팅풍선카테터의 개발 동향

박정훈 ( Junghun Park ),조원일 ( Won-il Jo ),변대흥 ( Dae-heung Byeon ),강소영 ( So Young Kang ),남충현 ( Choong-hyun Nam ),서경우 ( Kyoung-woo Seo ),박준규 ( Jun-kyu Park ) 한국공업화학회 2022 공업화학 Vol.33 No.5

급격한 고령화 사회로의 진입과 서구화된 식습관에 의해 협심증, 심근경색증, 고혈압 등에 의한 심장질환자의 수가 5년간 10만명 이상 증가했다. 심장질환은 지난 8년간 꾸준히 국내 사망원인 2위를 기록 중이며, 이로 인해 연간 소모되는 의료비는 6조원에 달한다. 증가한 환자와 함께 다양한 치료법이 제시되고 있는 가운데, 현재 보편화 된 관상동맥 중재시술 중 스텐트 삽입술의 단점인 재협착이나 장기간 이중항혈소판제 복용으로 인한 출혈의 위험 증가 등의 문제점을 보완하기 위해 약물코팅풍선카테터가 개발되어 점차 상용화되고 있는데, 국내에선 2010년 ‘SeQuent Please^Ⓡ (비브라운코리아)’라는 제품이 처음 출시되면서 약물코팅풍선카테터를 사용한 시술이 시작되었고, 이후 2012년 보험급여가 적용되면서 그 사용량이 본격적으로 늘어났다. 약물코팅풍선치료는 국내뿐만 아니라 전 세계적으로 사용이 증가하고 있는데, 특히 일본을 포함한 아시아태평양 지역에서 그 사용량이 급격히 증가하고 있다. 현재까지 국산 제품의 수요가 증가하고 있는 상황이며 향후, 동물시험 및 임상시험에서의 성능이 입증된다면 위험성이 높은 스텐트 삽입술에 비해 효과적인 시술이 될 것이라 예상한다. As a result of the rapid entry into an aging society and westernized eating habits, the number of heart disease patients caused by angina pectoris, myocardial infarction, and high blood pressure has increased by more than 100,000 over five years. Heart disease has consistently ranked second in the cause of death in Korea over the past eight years, and medical expenses consumed annually reach 6 trillion won. While various treatments are being proposed for more patients, drug-coated balloon catheters have been developed and gradually commercialized to solve the disadvantages of stent implantation such as restenosis and increased risk of bleeding due to long-term double antiplatelet medication. In Korea, it began to use a drug-coated balloon catheter with the first release of it called “SeQuent Please^Ⓡ (Bibrown Korea)” in 2010. Its demand increased gradually as insurance benefits were applied in 2012. Drug-coated balloon angioplasty is increasing in use not only in Korea but also around the world, especially in the Asia-Pacific region, including Japan. Until now, the demand for domestic products is increasing, and if the efficiency in vivo and clinical trials is proven in the future, it is expected to be an effective procedure compared to high-risk stent implantation.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

사용후핵연료 관리시설에 대한 지역수용성 결정요인 분석

김태현(Kim, Tae-Hyun),박현주(Park, Hyun-Joo),문지원(Moon, Ji-Won) 대한국토·도시계획학회 2016 國土計劃 Vol.51 No.5

Spent nuclear fuel repository has almost reached its maximum capacity in Korea. Recently, the issue of safe management and conflict resolution in decision-making process for spent nuclear fuel repository drew attention. The purpose of this research is to examine factors determining local acceptance of spent nuclear fuel repository and to investigate moderating effect among different groups of people. The survey was conducted to analyze the residents’ perception of spent nuclear fuel repository, and five factors were used to measure the local acceptance utilizing structural equation model. As a result, environmental impact and economic feasibility had a high positive relation to local acceptance rather than risk perception. In particular, the effects of environmental impact were distinctively high regardless of demographic characteristics. The degree of economic feasibility in male and household income less than 3 million won groups showed high effects on local acceptance, and risk perception had high effects on female and household income more than 3 million won. The research is significant to seek various factors that residents are concerned about and supports for policy-making.

Clinical usefulness of infrared thermal imaging as a non-invasive screening test for diagnosis of vascular malformations

( Chihyeon Sohng ),( Jun Hong Park ),( Won Kee Lee ),( Jun Young Kim ),( Yong Hyun Jang ),( Weon Ju Lee ),( Seok-jong Lee ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Infrared thermal imaging (ITI), aka thermography, has been used widely in medicine, but been studied rarely its clinical application in vascular malformations (VMs). Objectives: This study investigated validity and usefulness of ITI as a new screening test to differentiate between capillary malformation (CM) and arteriovenous malformation (AVM). Methods: This study included 50 patients with VMs who conducted ITI between 2011 and 2018. They were divided into two groups; CM and AVM, and those with Parkes-Weber syndrome (PWS) were also included in AVM group. Temperature difference (ΔT) between lesional and contralateral non-lesional area was compared using ITI to calculate the maximum ΔT (ΔTmax). The receiver operating characteristic (ROC) curve was then plotted to determine optimal cutoff value of ΔTmax. Results: Of the total 50 patients, 34 were finally diagnosed with CM, and 16 AVM (12 pure AVM, and 4 PWS). AVM group had significantly higher ΔTmax (2.57±1.23°C) than CM group (0.69±0.50°C).The area under the curve of ΔTmax for diagnosis of AVM versus CM was 0.938. The sensitivity and specificity were showed 87.5% and 94.1% (LR+, 14.87; LR-, 0.13) in cutoff value of ΔTmax 1.39°C, and 81.3% and 97.1% (LR+, 27.62; LR-, 0.19) in cutoff value of ΔTmax 1.42°C. Conclusion: The value of ΔTmax measured by ITI was useful to distinguish AVM from CM, so ITI can be employed as a new non-invasive differential screening test for uncertain VMs.