RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

素問·繆刺論에 對한 硏究(Ⅱ)

柳太植,鄭憲瑩,琴坰樹,朴炅 대한한의진단학회 1998 大韓韓醫診斷學會誌 Vol.2 No.2

The Scripture, the research on The MuJa Ron(○刺論) of the So Moon(素問), is described in the MuJa(○刺) of the Kap U1 Kyung(甲乙經). Also it is written in MuJa of Tae So(太素) volume 23,. And Wang(王○) interpreted it in volume 18 chapter 63. In ChimJaRyu(鍼刺類) of RyuKyung(類經) volume 20, it is introduced. And it is mentioned in the 2nd chapter of ShinKyoJung(新校正). In MuJa(○刺), Mu(#) means crossing left and right. Since this paper is describing the differences between MuJa(○刺) and KeoJa(巨刺), and it is also mentioning about carrying out the idea into practice, it is named MuJa(○刺). This paper, the theory of MuJa(○刺), can be divided into three properties in content. Chapter Ⅰ. deals with the common points and differences comparing with KeoJa(巨刺). Chapter Ⅱ. treats the idea that since RakMaek(絡脈) which is invaded by pathogenic factors are different, the states of the disorder are appeared differently so the place, method, atid number and order of acupuncture should be different. In chapter Ⅲ. it is said that in the state of disease of Kyung(經), KeoJa(巨刺) should be applied and in the state of the disease of Rak(絡), MuJa(○刺) should be used. treating oppositThe metabiosis of pathogenic factors flows from the skin and bodyhair to SonRak(孫絡), SonRak(孫絡) to RakMaek(絡脈), RakMaek(絡脈) to internal organs in general. In this situation KeoJa(巨刺) could be used in general. But if pathogenic factors flows irregularly, overflowing from one RakMaek(絡脈) to another place rather than internal organs, MuJa(○刺) could be applied. Therefore in the acupuncture treatment, the theory of MuJa(○刺) which implies side of the troubled places rather than the site itself is very important. But MuJa Ron(○刺論) is too difficult to interpret since is written in old Chinese, the writer of this paper has researched to prove the exact meaning of the MuJa(○刺).

만성신부전 환자에서 Recombinant Human Erythropoietin(rhEPO)의 피하주사시 생체이용율

김경수,이경훈,이영수,김광호,김용식,신상구,박찬웅 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

원주지역에서 발생한 비외상성 심정지의 일주변화

박금수,김영식,이진응,임종천,이강현,임경수,황성오,최경훈 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To estimate the quality of the emergency medical services system of Wonju City, we studied the diurnal variations of 179 non-traumatic cardiac arrest victims who received cardiopulmonary reuscitation at the emergency center of Wonju Christian Hospital. Diurnal variations of non-traumatic cardiac arrest patients were as follows ; The occurence of cardiac arrest at day-time was higher than night-time; 18 cases (11%) from midnight to AM 4, 25 cases (14%) from AM 4 to AM 8, 42 cases (24%) from AM 8 to AM 12, 46 cases (25%) from AM 12 to PM 4, 35 cases (19%) from PM 4 to PM 8, 13 cases (7%) from PM 8 to midnight. Witness cardiac arrest was increased more during the day than night ; 40% from midnight to AM 4, 48% from AM 4 to AM 8, 57% from AM 8 to AM 12.52% from AM 12 to PM 4, 60% from PM 4 to PM 8, 38% from PM 8 to midnight. The transportation time at night-time cardiac arrest was more longer than day-time cardiac arrest ; 30±12mins from midnight to AM 4, 26±9mins from AM 4 to AM 8, 27±12mins AM 8 to Am 12, 25±11mins from AM 12 to PM 4, 25±9mins from PM 4 to PM 8, 35±15mins from PM 8 to midnight. The rate of restoration of spontaneous circulation(ROSC) in day-time cardiac arrest was higher than the night-time cardiac arrest ; 30% from midnight to AM 4, 36% from AM 4 to AM 8, 32% AM 8 to AM 12, 44% from AM 12 to PM 4, 41% from PM 4 to PM 8, 15% from PM 8 to midnight. The survival rate of cardiac arrest has been correlated with collapse time, early bystander CPR, early advanced care. To improve outcome for prehospital cardiac arrest, we concluded that early bystander CPR, and early advanced life support should be performed at the scene and during the transportation especially at night.

Methotrexate가 脾臟에 미치는 影響에 관한 硏究

朴景蘭,李永浩,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

In order to study the morphological changes of spleen after treatment with methotrexate, ICR mice were used as experimental animal. The experimental mice were divided into control, 50mg/kg and 500mg/kg of methotrexate treated groups. Methotrexate injected intravenously via tail vein once on the 8th day of pregancy. The mice were sacrificed on the 1st, 2nd 3rd and 4th day after treatment, the spleens were removed and fixed in 10% neutral buffered formalin solution for 48hrs. Spleens were cutted 5㎛ thick and stained with H-E, then observed under the light microscope. The results were as follows: 1. The size of spleen were reduced after MTX treatment more on dose day by day. 2. The periarterial lymphatic sheath (PALS) and germinal center were also reduced after MTX treatment. 3. The lymphocytes of white pulp showed nuclear pyknotic changes and many nuclear debris were found after MTX treatment. 4. The brown hemosiderin pigments were increased markedly in the red pulp of MTX treated groups. 5. Marked proliferation of fibrous trabeculae were found in the red pulp of MTX treated mice.

BCG를 대량 투여 받은 생쥐 가슴샘 겉질의 미세구조

고정식,안의태,박경호,우성임,한영복,경홍기 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

고장으로 인한 대구경 계량기의 불감률

박남식,배정용,황경석,김학용,최철식 대한상하수도학회 2003 상하수도학회지 Vol.17 No.2

Leaks and meter under registrations are suspected as primary components of unaccounted-for water in Korea. Roughly, one third of the unaccounted-for water is believed to be due to meter under registration. However, no justification has been given to prove the statistics. In this study the rate of meter under registration due to failures of large-diameter meters is investigated. Replacement records of over 2600 consumer sites are analyzed. The minimum, the maximum and the probabilistic distribution of meter under registration rate are computed.

성별판별을 위한 한국인 성인 방패연골의 판별분석

박대균,안의태,고정식,박경호 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

방패연골은 목뿔뼈 바로 아래 위치하며 후두부를 구성하는 연골 가운데 가장 큰 연골이다. 본 연구는 한국인 방패연골의 형태분석을 통해 얻은 결과를 이용하여 성별판별을 할 수 있는지 알아보고자 하였다. 20대이상 한국인에서 109(남자69, 여자 40)개의 방패연골을 대상으로 26가지 항목을 계측하여 통계학적으로 검증하여 다음과 같은 결과를 얻었다. 1. 방패연골너비, 방패연골높이, 방패연골길이, 연골판높이 등은 남자의 계측치가 컸다 (p<0.01). 2. 연골판각, 방패연골아래뿔각, 위방패패임각 등은 여자의 게측치가컸다. (p<0.01). 3. 방패연골길이와 연골판높이 항목을 이용하여 남녀판별함수를 얻었다. 이상의 결과에서 한국인 방패연골은 성별에 따라 차이가 있었으며, 20대 이상 한국인의 경우 방패연골의 형태분석에 의해 성별을 판별할 수 있을 것이다.

X-선 조사를 받은 흰쥐망막 빛감각세포의 미세구조

고정식,박동분,양남길,안의태,박경호,김진국 대한해부학회 1993 Anatomy & Cell Biology Vol.26 No.3

발꿈치힘줄이 절단된 흰쥐의 가자미근과 장단지근내의 모세혈관과 신경섬유의 형태학적 연구

고정식,최기수,양남길,안의태,박경호,김진국 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.1