RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

素問·繆刺論에 對한 硏究(Ⅱ)

柳太植,鄭憲瑩,琴坰樹,朴炅 대한한의진단학회 1998 大韓韓醫診斷學會誌 Vol.2 No.2

The Scripture, the research on The MuJa Ron(○刺論) of the So Moon(素問), is described in the MuJa(○刺) of the Kap U1 Kyung(甲乙經). Also it is written in MuJa of Tae So(太素) volume 23,. And Wang(王○) interpreted it in volume 18 chapter 63. In ChimJaRyu(鍼刺類) of RyuKyung(類經) volume 20, it is introduced. And it is mentioned in the 2nd chapter of ShinKyoJung(新校正). In MuJa(○刺), Mu(#) means crossing left and right. Since this paper is describing the differences between MuJa(○刺) and KeoJa(巨刺), and it is also mentioning about carrying out the idea into practice, it is named MuJa(○刺). This paper, the theory of MuJa(○刺), can be divided into three properties in content. Chapter Ⅰ. deals with the common points and differences comparing with KeoJa(巨刺). Chapter Ⅱ. treats the idea that since RakMaek(絡脈) which is invaded by pathogenic factors are different, the states of the disorder are appeared differently so the place, method, atid number and order of acupuncture should be different. In chapter Ⅲ. it is said that in the state of disease of Kyung(經), KeoJa(巨刺) should be applied and in the state of the disease of Rak(絡), MuJa(○刺) should be used. treating oppositThe metabiosis of pathogenic factors flows from the skin and bodyhair to SonRak(孫絡), SonRak(孫絡) to RakMaek(絡脈), RakMaek(絡脈) to internal organs in general. In this situation KeoJa(巨刺) could be used in general. But if pathogenic factors flows irregularly, overflowing from one RakMaek(絡脈) to another place rather than internal organs, MuJa(○刺) could be applied. Therefore in the acupuncture treatment, the theory of MuJa(○刺) which implies side of the troubled places rather than the site itself is very important. But MuJa Ron(○刺論) is too difficult to interpret since is written in old Chinese, the writer of this paper has researched to prove the exact meaning of the MuJa(○刺).

만성신부전 환자에서 Recombinant Human Erythropoietin(rhEPO)의 피하주사시 생체이용율

김경수,이경훈,이영수,김광호,김용식,신상구,박찬웅 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

腎兪 熱地黃 水鍼이 正常 및 實驗的 腎性高血壓 白鼠의 腎臟機能에 미치는 影響

朴呈培,朴喜守,김홍일,李昊燮,金庚植 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

To investigate the effect of Shudihuang(熟地黃) aqua-acupuncture at the meridian points BL 23 on the renal function in normal and two kidney one clip Goldblatt hypertensive rats. The results of this study were as follows : 1. Urine volume, free water clearance rate were significant increased after Shudihuang aqua-acupuncture in normal rats. 2. Urinary excretion on potassium and chloride, glomerular filtration rate were significant decreased after Shudihuang aqua-acupuncture in normal rats. 3. Urine volume, ruinary excretion of electrolytes were significant decreased after Shudihuang aqua-acupuncture in two kindney one clip Goldblatt hypertensive rats. 4. Free water clearance, glomerular filtration rate were significant increased after Shudihuang aqua-acupuncture in two kidney one clip Goldblatt hypertensive rats.

건축물 3종 안전점검 사례분석에 따른 기법 및 제도 개선에 관한 연구

Park,In Seo,Jeon,Kyung Sik 경기대학교 재난안전연구소 2020 재난안전논문집 Vol.2020 No.1

연구목적 : 본 연구는 건축물 3종 정기안전점검의 현장점검·보고서의 개선을 목적으로 한다. 연구방법 : 연구를 위해 건축물 3종 정기안전점검의 현장점검·보고서 문제점을 진단점검 기술자에게 5점 리커트 척도로 구성하여 79명을 대상으로 설문조사를 실시하였다. 연구결과 : 현장 구조체 외부에 마감재로 시공되어있어 구조체의 확인이 불가한 상태이다. 이로 인하여 점검대상 시설물의 현장조사시 결함여부에 대한 조사가 곤란하여 객관적인 평가에 문제점이 많다. 따라서 최초 설계시에 유지관리을 고려한 마감재선정, 점검구 배치가 바람직하다. 주요구조부에 대한 추가적인 점검구설치, 리모델링공사시 점검구 설치 등 이 필요하다. 복사 붙여넣기식 보고서는 많은 수의 보고서 작성시 유사한 결함과, 기존에 점검시에 결함내용과 동일한 결함조사 내용으로 판단하고 점검기술자가 보고서를 작성하게 되므로 인하여 건물특성에 적합하고, 유지관리에 도움이 되지않는 문제점이 있다. 결론 : 관리주체의 의식교육, 마감재에 의한 구조체 확인한계, 점검기술자의 교육, 저가수주에 의한 기술자 및 점검시간 부족방지를 위해 대가의 현실화, 3종 시설물 평가 Check list의 개선, 시설물 관리주체의 의도에 따른 점검, 복사 붙여넣기식 보고서, 종합결론과 조사내용과 불일치에 대한 개선이 필요한 것으로 나타났다. Purpose: This study aims to improve on-site inspection and report of three-classes buildings. Method: For the purpose of the study, problems with field inspection and report of three-classes buildings were organized into five-point clock scale for the diagnostic inspection engineer and conducted a survey of 79 people. Results: The structure cannot be verified as it is constructed with finishing materials outside the site structure. As a result, there area many problems in objective evaluation as it is difficult to investigate whether a facility is defective during an on-site inspection. Therefore, it is desirable to re-select the finish and deploy the inspection tools considering maintenance at the time of initial design. Additional inspection tools for major structural parts and installation of inspection tools for remodeling work are required. Copying and pasting reports are considered to be similar in the preparation of a large number of reports, and are considered to be the same defect investigation details as the previous inspection results, and the inspection engineer prepares the report, which is suitable for building characteristics of the building and is not conducive to maintenance. Conclusion: It was found that improvement of consciousness education of the managing body, verification of structure by finishing materials, education of inspection engineers, improvement of the checklist of three types of facilities evaluation, inspection of the intention of the managing body of the facility, and comprehensive conclusion and survey contents and inconsistency are needed to prevent shortage of inspection time.

공업용 접착제 증기 흡입이 흰생쥐 기관상피에 미치는 영향 : 조직화학적 및 미세구조적 연구

박경호,박삼열,고정식,안의태,박대균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Industrial glues, known as Bonds in Korea, contain many kinds of organic solvents, and glue sniffing of youths became one of the social problems in Korea. Mixed exposures to solvents by glue sniffing may induce chronic toxicities different from those induced by exposures to the solvent of single component. In this study, toxicological insult of the inhalation of glue vapor on the primary target organ, the tracheal epithelium of the respiratory system was morphologically worked out. Vapor samples of organic solvents were collected for analysis, and the components found were aceton, n-hexane, methyl cyclopentane, c-hexane, and toluene by gas mas. In order to inhale the organic solvents of adhesives to the mouse, experimental animals were exposed to vapors of the glues(acetone 5212 ppm, n-hexane 139 ppm, c-hexane 3757 ppm, methylcyclopentane 1820 ppm, and toluene 2.2 ppm) in a chamber for 20 min/day for 3, 5, 7, 14 consecutive days, in a whole body inhalation chamber. Control groups were exposed to room air. Animals were sacrificed and tracheal tissues were collected and fixed in 10% neutral formalin for light microscopy and in 2.5% glutaradlehyde - 1.5% paraformaldehyde for transmission electron microscopy and in 2.5% glutaraldehyde for scanning electron microscopy. The results are as follows : 1. In the tracheal epithelium of a normal mouse, ciliated cells, nonciliated cells and basal cells were found, but goblet cells and submucosal glands were not found. 2. In the tracheal mucosa of a normal mouse, alcianophilic bands were detected, but PAS positive bands were not found, but alcian blue-PAS positive cells were found in the mucosae exposed to glue vapor for 7 days and 14days. 3. Distributing of ciliated and nonciliated cells in the epithelium were roughly comparable during experimental periods; the longer the experimental period(2 week), higher the percentage of non-ciliated cells. 4. The mouse of organic solvent inhaled group showed three distinct forms of nonciliated cells, which were apparently transitional types between nonciliated cells and ciliated cells. It is suggested that the role of the nonciliated cell might be a developmental functioning involved in the epithelial cell turnover. Nonciliated cells show a great deal of plasticity; change to ciliated cells during the early phase; and revolving of nonciliated cells during the repopulation phase after injury. The results suggest that ultrastructural morphologic changes in tracheal epithelia may occur following glue sniffing, and those structural changes were analysed as an adaption to the environment.

思考技能 訓練프로그램의 開發 및 適用에 關한 硏究 : 衝動性 減少를 中心으로

朴鎭玹,朴敬玉,申東魯,羅東晉,李永植 全北大學校 學生生活硏究所 1994 學生生活硏究 Vol.21 No.-

Many of college students have the cognitive deficits, one of which is the impulsiveness, in the process through which they solve their problems in the daily life. This study attemted to develop the program for the purpose of improving college students' thinking skills. Also, the program was intended to encourage the college students to reduce their impulsiveness, and to help them maintain the rational reasoning solving the complex of problems. For this purpose, researchers examined the difficulties which college students had in the process of their thinking, focussed on their impulsiveness in particular, based on the findings of preceding works related to the thinking skills, including some of programs for improving the thinking skills and intelligence. As a result, a program was developed to improve the thinking skills of college students in order to reduce their impulsiveness. This program was composed of two parts, each of which included five units. Part One, titled "Human Life and the Limited Rationality", was focused on training college students with metacognition or cognitive style. In this part, the trainees explored those phases passed on the human problem solving and the sources of impulsiveness. This part included five units: (1) Introduction; (2) Journey in the universe of learning; (3) Uncertainty and impulsiveness; (4) Human problem solving; and (5) Human information processing and limited rationality. Part Two, titled "Resolution of the Limited Rationality and Intellectual Limitations of Human Being", was focused on training college students with cognitive strategies. In this part, the trainees searched for the general cognitive strategies with which they would learn each unit, and used it to resolve the limited rationality of human being and thus to solve the problems more efficiently. This part included five units: (1) Search for strategies to resolve the intellectual limitations of human being; (2) Utility of the external representations; (3) Application of strategic knowledge of inferences; (4) Resolution of the inferential baises; and (5) Evaluation and termination. A pilot study attempted to verify the effectiveness of the training program. Subjects were 118 sophomores of a national university in Chon-buk. They were assigned to an experimental group(N=56) and a control group(N=62) to test the effectiveness of the training program. The experimantal group received 12 sessions of training instruction for 50 to 60 minutes each week. The control group was given no intervention. After training sessions, they were given a cognitive style (impulsiveness) scale as well as a reasoning test, and also checked a questionnaire which asked the participants to express their opinions of the training program. The findings of the study might be summarized as follows: 1. The program seemed to contribute posively to change participants' cognitive style, particularly to reduce their dysfunctional impulsiveness. 2. The program seemed to contribute substantially to improve participants' performance of reasoning tasks. 3. Many of participants (about tow-third) of the participants reported to have a novel feel- ing on the program, to perceive the program different from the existing curriculum, and also to take some benefits from the program. However, this study suggested several recommandations as follow: 1. It is necessary to verify the effectiveness of the program on the basis of a valid design of experiment. 2. It is necessary to take a effort to extend the breadth and depth of the program, including a discussion of the functional impulsiveness. 3. It is necessary to establish the reinforcement schedule in order to encourage the participant to engage in the program actively.

토끼 눈물샘에서 Nitric Oxide Synthase의 면역조직화학적 연구

박경호,이석호,고정식,안의태,박대균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Endogenous nitric oxide(NO) has been known to regulate many physiological and pathological process, especially the salivary secretion and glandular blood flow. However, nitric oxide synthase(NOS) responsible for NO synthesis has not been well studied in rabbit lacrimal gland. The present study was performed to investigate the distribution of nitric oxide synthase isoforms(endothelial, neuronal, and inducible NOS). Immunohistochemistry, using monoclonal mouse anti-endothelial NOS, anti-neuronal NOS, and anti-inducible NOS, was performed in inferior lacrimal of the rabbit. Endothelial NOS(eNOS)-positive immunoreactivities were observed in arterial endothelia, intralobular ducts and interlobular ducts of the inferior lacrimal gland of the rabbit. eNOS-positive immunoreactivities were most prominent in the interlobular and intralobular ducts. Immunoreactivities were well concentrated in cytoplasm of columnar epithelial duct cells. However, weak eNOS-positive immunoreactivity was observed in the intercalated ducts and vascular endothelial cells. Neuronal NOS(nNOS)-positive immunoreactivity was seen in the nerve fibers of inferior lacrimal gland. nNOS-positive immunoreactivy was not detected in duct systems or in acinar cells. Inducible NOS-positive immunoreactivity was not seen. Especially, the expression of the nNOS in the nerves of lacrimal gland suggest that NO may play a role in modulating tear production. These results reveal the presence of eNOS and nNOS in the lacrimal gland of the rabbit, which may be related with regulation of the glandular secretion and blood flow through the gland.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

檢査資料의 活用을 통한 集團指導 活動事例의 效果評價

朴敬玉,李永植,宋在鴻 全北大學校 學生生活硏究所 1992 學生生活硏究 Vol.19 No.-

This study was attempted to introduce the group guidance activity, in which the guide has provided the freshmen with their results of psychological tests and has encouraged to improve their self-understanding, and to examine the effectiveness of that activity. In this study, investigators have highlighted the historical background of the group guidance activity through using the results of psychological tests, and have given and outline of the practice of present activity, including 1) the understanding of human behavior and the functions of psychological testing in the base of the information-processing approach ; 2) the main points in interpreting the results of psychological tests and the implications of individual test for self-understanding ; 3) the general strategies for identifying the problems of individuals and coping with them ; and 4) the introduction to the purpose and activities of the Student Guidance and Counseling Center at Chonbuk National University and the guide to use the Center. And then they have examined the direct and/or indirect effectiveness of that activity. This study has suggested several recommandations for the future activity as follows : 1. It is necessary to examine earnestly the purpose and contents of psychological tests used in the group guidance, so that students may search the self-understanding actively. 2. It is necessary to make an effort in helping the students identify the practical problems, specific to psychological adjustment such as interpersonal relationships, adademic performance, career development, and so on, and cope with them. 3. It is necessary to evaluated continuously the effectiveness of the group guidance activity through using the result of psychological tests, and it is followed by formulating a plan which may result in the improvement of that activity in future.