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Nara Lee,Keun Young Huh,Jonghoon Park 한국인간·식물·환경학회 2017 인간식물환경학회지 Vol.20 No.4
The purpose of this study was to investigate the recognition and demands of forest interpretation programs. Questionnaire survey was carried out to estimate the awareness, necessity, future participation intent, experience, satisfaction and demands of forest interpretation programs with 100 residents living in Jinju city or Gyeongnam region. The data were subject to frequency analysis, correlation analysis, and path analysis. Overall 63% of respondents didn’t know forest interpretation programs, but 52% of respondents recognized the necessity of forest interpretation programs. 52% of respondents had the future participate intent of forest interpretation programs, and 38.3% didn’t know if they would participate. 74% of respondents had never experienced the programs. In the satisfaction, 80% of respondents who experienced showed neutral. Future participation intent was highly correlated with necessity as shown in the result of correlation analysis among awareness, necessity, future participation intent, and experience of forest interpretation programs. Also future participation intent in path analysis was estimated to be affected indirectly by experience and awareness as well as necessity. Enhancement of future participation intent would be achieved by increasing experience, awareness, and necessity of forest interpretation programs. According to the demands, forest interpreters were required to have the expertise to provide knowledge and information of forests. Attractive content for forest interpretation programs was in the order of recreational activities > forest conservation > education while the proper objective of forest interpretation programs was experience and interest > education > conservation and resource > public relation of forests and parks. Respondents preferred participation and experience the most as a style in selecting the programs. Key condition in selecting the venue was diversity > safety > connectivity > accessibility in order. There was no significantly different preference in proper participation duration or operation season for the activities of forest interpretation programs.
Flow Cytometry-Based Quantification of Cellular Au Nanoparticles
Park, Jonghoon,Ha, My Kieu,Yang, Nuri,Yoon, Tae Hyun American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.4
<P>There has been a great deal of research regarding the cellular association of nanoparticles (NPs), although there are only a few methods available yet for the quantitative measurements of cellular NPs. In this study, we propose a simple and quantitative method to estimate the cellular uptake of Au NPs into cervical cancer cells (HeLa) based on their side scattering (SSC) intensities measured by flow cytometry (FCM). We have compared SSC intensities of HeLa cells exposed to eight different types of Au NPs (40-100 nm size, with positive or negative surface charge) with the amount of cellular Au NPs measured by inductively coupled plasma mass spectrometry (ICPMS). On the basis of these comparisons, we have found linear correlations between the cellular Au NPs and the SSC intensities and used them to estimate the amount of Au NPs associated with HeLa cells. Once the correlations were found for specific cell lines and types of nanoparticles, this approach is useful for simple and quantitative estimation of the cellular Au NPs, without performing labor-intensive and complicated sample preparation procedures required for the ICPMS approach.</P>
( Jonghoon Park ),( Kazuko Ishikawa Takata ),( Sangjik Lee ),( Eunkyung Kim ),( Kiwon Lim ),( Hyungryul Kim ),( In Sook Lee ),( Shigeho Tanaka ) 한국운동영양학회 2016 Physical Activity and Nutrition (Phys Act Nutr) Vol.20 No.3
[Purpose] The purpose of the current study was to investigate steps per day (steps/d) and physical activity level (PAL) in Korean elementary school children having normal weight (normal-weight). We also clarified whether a gender difference exited between steps/d and PAL. [Methods] Children aged 9 to 12 y were recruited from two elementary schools located in different urban districts in Korea. The present study included 33 Korean children, of which 18 were normal-weight boys and 15 were normal-weight girls. During the same 1 week study period under free-living conditions the total energy expenditure (TEE) and step counts were estimated using the doubly labeled water (DLW) method and an accelerometer, respectively. We calculated PAL as the TEE/ resting metabolic rate. [Results] The range of PAL was 1.25 - 1.93 with a mean value of 1.57. None of the variables of energy expenditure was significantly different by sex. However, steps/d were significantly higher in boys than in girls. When adjusting regression analysis by gender, steps/ d were positively associated with PAL among all subjects (r = 0.56, P < 0.01). Furthermore, steps/d were positively associated with PAL in boys (r = 0.68, P < 0.01), but not in girls (r = 0.27, P = 0.34). [Conclusion] Our results suggest that locomotive activity may be the main contributor to the individual PAL differences for elementary school boys, while non-locomotive activity may be the main contributor for elementary school girls.
Microfluidic Image Cytometry (μFIC) Assessments of Silver Nanoparticle Cytotoxicity
Park, Jonghoon,Yoon, Tae Hyun Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.12
Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (${\mu}FIC$). The $LC_{50}$ value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the ${\mu}FIC$ approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This ${\mu}FIC$ method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty-toxicity relationships.
Microfluidic Image Cytometry (μFIC) Assessments of Silver Nanoparticle Cytotoxicity
Jonghoon Park,Tae Hyun Yoon 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.12
Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (μFIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/ L). These results confirm that the μFIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This μFIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.
Interpolation and Tracking of Rigid Body Orientations
Jonghoon Park 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10
A method of interpolating and tracking rigid body motions in terms of its exponential coordinates is developed based on the differential of exponentials. The analytic closed-form expressions are provided for differential of exponential on SO(3) as well as its time-derivative. Complete methods for interpolating and tracking in terms of exponential coordinates of SO(3) are developed. In particular, interpolation is made with the boundary conditions including acceleration level. Reference acceleration is designed on SO(3) for linearized closed-loop dynamics in exponential coordinates.