비외상성 두개내 출혈 환자에서 심근손상의 발생과 예후에 미치는 영향

강구현,황성호,이강현,조준휘,김성환,문중범,박해상,이서영,이성수,김헌주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Objective: The aim of this study was to investigate the clinical significance of myocardial injuries in patients with nontraumatic intracranial hemorrhage by identifying the occurrence of myocardial injury and defining its correlation with subsequent cardiovascular events. Subjects and methods: One hundred twenty-four patients with nontraumatic intracraninal hemorrhage presented to the emergency department within six hours from onset of symptoms were enrolled. Brain CT, serial electrocardiography, and echocardiography were done at the emergency center. Blood samples for troponin I and creatine kinase(CK)-MB were drawn immediately and eight hours after admission, Troponin I and CK-MB were measured using a chemiluminescent immunoassay, respectively. Results: Electrocardiographic and echocardiography abnormalities were found in 65 cases(52.4%) and 21 cases(17%), respectively. Serum troponin I and creative kinase-MB were increased in 35 cases (28.2%) and in 58 cases(46.8%), respectively. Abnormal findings of echocardiography and ECG, as well as elevated levels of serum troponin I and creative kinase-MB, were associated with an increased risk of cardiovascular event and survival. Logistic regression analysis revealed that an abnormal echcocardiographic finding and elevation of serum troponin I were factors associated with the occurrence an adverse cardiovascular event and that electrocardiographic abnormalities and initial mental status were factors associated with poor prognosis. Conclusion: This study reveals that actual myocardial injury develops in a significant proportion of patients with nontraumatic intracranial hemorrhage and that the development of the myocardial injury is associated with an adverse cardiovascular event that occurs during admission.

CURRENT STATUS OF NUCLEAR FUSION ENERGY RESEARCH IN KOREA

MYEUN KWON,YOUNG SOON BAE,SEUNGYON CHO,최원호,홍봉근,황용석,JIN YONG KIM,KEEMAN KIM,YAUNG-SOO KIM,JONG-GU KWAK,HYEON GON LEE,SANGIL LEE1,나용수,BYUNG-HOON OH,YEONG-KOOK OH,박지연,HYUNG LYEOL YANG,IN KEUN YU 한국원자력학회 2009 Nuclear Engineering and Technology Vol.41 No.4

The history of nuclear fusion research in Korea is rather short compared to that of advanced countries. However, since the mid- 1990s, at which time the construction of KSTAR was about to commence, fusion research in Korea has been actively carried out in a wide range of areas, from basic plasma physics to fusion reactor design. The flourishing of fusion research partly owes to the fact that industrial technologies in Korea including those related to the nuclear field have been fully matured, with their quality being highly ranked in the world. Successive pivotal programs such as KSTAR and ITER have provided diverse opportunities to address new scientific and technological problems in fusion as well as to draw young researchers into related fields. The frame of the Korean nuclear fusion program is now changing from a small laboratory scale to a large national agenda. Coordinated strategies from different views and a holistic approach are necessary in order to achieve optimal efficiency and effectiveness. Upon this background, the present paper reflects upon the road taken to arrive at this point and looks ahead at the coming future in nuclear fusion research activities in Korea.

Evaluation of the Oral Acute Toxicity of Black Ginseng in Rats

Lee, Mi-Ra,Oh, Chang-Jin,Li, Zheng,Li, Jing-Jie,Wang, Chun-Yan,Wang, Zhen,Gu, Li-Juan,Lee, Sang-Hwa,Lee, Jae-Il,Lim, Beong-Ou,Sung, Chang-Keun The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.1

We studied the acute oral toxicity of black ginseng (BG) produced by heat process in rats. Single acute BG extract doses of 0, 5, 10, and 15 g/kg dissolved in saline were administered by oral gavage and the animals were kept under observation for 14 days. The single administration of BG extract up to 15 g/kg did not produce mortality, behavioral change or abnormal clinical signs in the rats. These results indicated that the oral $LD_{50}$ of the BG extract in the rats is higher than 15 g/kg. Compared to the control group, no treatment-related biologically significant effects of BG extract were noted in the measurements of the body weight or food intake. At the end of the period, the biochemical parameters and hematological parameters were analyzed in the plasma and blood. A histopathological examination of the liver and kidney was also conducted. Only the blood nitrogen urea and potassium levels in the biochemical indices showed significant differences at 10 and 15 g/kg doses of BG extract compared to the control group. These changes were not considered to be due to the toxicity. None of the other clinical chemistry parameters were affected. Therefore, these results indicate that the BG by heat processing is virtually nontoxic.

Removal and Recovery of Cr(VI) from Industrial Plating Wastewater Using Fibrous Anion Exchanger

Lee, Chang-Gu,Park, Jeong-Ann,Choi, Jae-Woo,Ko, Seok-Oh,Lee, Sang-Hyup Springer Netherlands 2016 Water, Air and Soil Pollution Vol.227 No.8

<P>The aim of this study was to investigate the removal and recovery of hexavalent chromium (Cr(VI)) from industrial plating wastewater using anion exchanger Kanecaron SA fibers in batch systems. The surface morphology and physicochemical properties of the fiber were analyzed by scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDAX), and Fourier transform infrared spectroscopy (FT-IR). The removal efficiency was affected by the solution pH and showed a plateau formation decreasing on both sides of pH 4. The Cr(VI) uptake on Kanecaron SA fibers was rapidly increased in the first 10 min, and the kinetic data fit well to the Elovich model. Isotherm model analysis demonstrates that the Redlich-Peterson model suitably describes the equilibrium data, and the maximum adsorption capacity (Q(m)) from the Langmuir model was 87.366 mg/g for Cr(VI) in distilled water, 117.977 mg/g for total Cr, and 57.101 mg/g for Cr(VI) in wastewater. Additionally, the Cr(III) contained in the plating wastewater was removed by the Kanecaron SA fibers, while the other heavy metals were not removed. Thermodynamic analysis indicates that Cr(VI) adsorption to Kanecaron SA fibers decreased with increasing temperature from 10 to 50 degrees C, indicating the spontaneous and exothermic nature of the sorption process. The removal efficiency was maintained above 80 % during four regeneration cycles.</P>

Candesartan, an angiotensin-II receptor blocker, ameliorates insulin resistance and hepatosteatosis by reducing intracellular calcium overload and lipid accumulation

Lee Jin Wook,Gu Hyun-Oh,Jung Yunshin,Jung YunJae,Seo Seung-Yong,Hong Jeong-Hee,Hong In-Sun,Lee Dae Ho,Kim Ok-Hee,Oh Byung-Chul 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Insulin resistance is a major contributor to the pathogenesis of several human diseases, including type 2 diabetes, hypertension, and hyperlipidemia. Notably, insulin resistance and hypertension share common abnormalities, including increased oxidative stress, inflammation, and organelle dysfunction. Recently, we showed that excess intracellular Ca2+, a known pathogenic factor in hypertension, acts as a critical negative regulator of insulin signaling by forming Ca2+-phosphoinositides that prevent the membrane localization of AKT, a key serine/threonine kinase signaling molecule. Whether preventing intracellular Ca2+ overload improves insulin sensitivity, however, has not yet been investigated. Here, we show that the antihypertensive agent candesartan, compared with other angiotensin-II receptor blockers, has previously unrecognized beneficial effects on attenuating insulin resistance. We found that candesartan markedly reduced palmitic acid (PA)-induced intracellular Ca2+ overload and lipid accumulation by normalizing dysregulated store-operated channel (SOC)-mediated Ca2+ entry into cells, which alleviated PA-induced insulin resistance by promoting insulin-stimulated AKT membrane localization and increased the phosphorylation of AKT and its downstream substrates. As pharmacological approaches to attenuate intracellular Ca2+ overload in vivo, administering candesartan to obese mice successfully decreased insulin resistance, hepatic steatosis, dyslipidemia, and tissue inflammation by inhibiting dysregulated SOC-mediated Ca2+ entry and ectopic lipid accumulation. The resulting alterations in the phosphorylation of key signaling molecules consequently alleviate impaired insulin signaling by increasing the postprandial membrane localization and phosphorylation of AKT. Thus, our findings provide robust evidence for the pleiotropic contribution of intracellular Ca2+ overload in the pathogenesis of insulin resistance and suggest that there are viable approved drugs that can be repurposed for the treatment of insulin resistance and hypertension.

Cholesterol Oxidase를 생산하는 방선균분리주 HSL-613의 동정

이홍수,이인애,최용경,이희구,이근철,박용하,오태광,최인성,정태화 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.4

임상진단용 산화효소인 cholesterol oxidase를 생성하는 방선균 분리주 HSL-613을 토양으로부터 분리하여 ISP(International Streptomyces Project) 분리법과 화학적 방법으로 동정하였다. 분리주 HSL-613의 모양은 spiral 형태이며, 포자는 원형으로 smooth type이었다. 분리주 HSL-613의 세포벽 성분은 LL-DAP(diaminopimelic acid)로 나타났으며, chromosomal DAN의 평균 G+C 함량은 59%였고, 지방산은 주로 iso-branched와 anteiso-branched로 구성되었으며, menaquinone은 MK-9(H_6, H_8)을 가지고 있었다. 세포내의 당은 육탄당인 galactose, glucose, mannose, 그리고 오탄당인 robose로 구성되어 sugar pattern의 경우는 Streptomyces와는 전혀 다른 특징적 조성을 이루고 있었다. 5S rRNA의 염기서열을 sequencing한 결과 일반적으로 Actinomycetes의 경우 120개의 nucleotide를 가지고 있으며 본 분리주의 5S rRNA sequencing을 type strain인 Streptomyces griseus subsp. griseus KCTC 9080과 비교한 결과 120개의 nucleotide를구성하고 있으므로 형태학적, 생리학적, 화학적 특성을 조사하고 type strain과 비교하여 동정한 결과 분리주 HSL-613을 Streptomyces sp. HSL-613이라고 명명하였다. An actinomycete strain, HSL-613 was isolated from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H_6, H_8) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

Administration of encapsulated L-tryptophan improves duodenal starch digestion and increases gastrointestinal hormones secretions in beef cattle

Lee, Sang-Bum,Lee, Kyung-Won,Wang, Tao,Lee, Jae-Sung,Jung, U-Suk,Nejad, Jalil Ghassemi,Oh, Young-Kyoon,Baek, Youl-Chang,Kim, Kyoung Hoon,Lee, Hong-Gu Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: This study investigated the effects of oral administration of rumen-protected L-tryptophan (RPL-T) on duodenal starch digestion and gastrointestinal hormones (GIH) secretions using Hanwoo beef steers as the animal models. Methods: Four steers (423±24 kg) fitted with ruminal and duodenal cannulas were employed in a crossover design replicated twice. Treatments were control (basal diet) and RPL-T (basal diet+191.1 mg/kg body weight [BW]) group. Blood and duodenal samples were collected to measure serum GIH levels and pancreatic α-amylase activity at day 0, 1, 3, and 5 (-30, 30, 90, 150, and 210 min) of the study. Samples from each segment of the gastrointestinal tract were collected via ruminal and duodenal cannulas and were used to determine soluble protein and the starch digestion rate at days 6 (-30, 180, 360, and 540 min) and 8 (-30, 90, 270, and 450 min) of the experiment. Results: No significant difference in ruminal pH, NH₃-N, and total volatile fatty acid including the levels of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, and the acetate-to-propionate ratio was observed between groups (p>0.05). Crude protein uptake was higher and feces starch content was lower in RPL-T group than the control group (p<0.05). The D-glucose contents of feces in RPL-T group decreased at day 5 compared to those in the control group (p<0.05), however, no change was found at day 0, 1, or 3 compared to the control group (p>0.05). Serum cholecystokinin (CCK), melatonin, duodenal pancreatic α-amylase activity, and starch digestion were significantly higher in RPL-T group than the control group (p<0.05). Conclusion: Taken together, oral administration of RPL-T at the rate of 191.1 mg/kg BW consistently increased CCK concentration, pancreatic α-amylase activity in duodenal fluids, and starch digestion rate in the small intestine and thus found to be beneficial.

Efficacy of an Alpha-Blocker for the Treatment of Nonneurogenic Voiding Dysfunction in Women: An 8-Week, Randomized, Double-Blind, Placebo-Controlled Trial

Lee, Young-Suk,Lee, Kyu-Sung,Choo, Myung-Soo,Kim, Joon Chul,Lee, Jeong Gu,Seo, Ju Tae,Lee, Jeong Zoo,Lee, Ji Youl,Oh, Seung-June,Na, Yong Gil Korean Continence Society 2018 International Neurourology Journal Vol.22 No.1

<P><B>Purpose</B></P><P>To evaluate the efficacy of an alpha-1 adrenergic receptor (α1-AR) blocker for the treatment of female voiding dysfunction (FVD) through a pressure-flow study.</P><P><B>Methods</B></P><P>This was a randomized, double-blind, placebo-controlled trial. Women aged ≥18 years with voiding symptoms, as defined by an American Urological Association symptom score (AUA-SS) ≥15 and a maximum flow rate (Qmax) <15 mL/sec with a voided volume of >100 mL and/or a postvoid residual (PVR) volume >150 mL, were randomly allocated to either the alfuzosin or placebo group. After 8 weeks of treatment, changes in the AUA-SS, Bristol female lower urinary tract symptoms (BFLUTS) questionnaire, Qmax/PVR, and voiding diary were compared between groups. Patients’ satisfaction with the treatment was compared. Patients were categorized into 3 groups according to the Blaivas-Groutz bladder outlet obstruction (BOO) nomogram: none, mild, and moderate to severe. Subgroup comparisons were also made.</P><P><B>Results</B></P><P>Of a total of 187 women, 154 (79 alfuzosin, 75 placebo) were included in the analysis. After 8 weeks of treatment, the AUA-SS decreased by 7.0 in the alfuzosin group and by 8.0 in the placebo group. Changes in AUA-SS subscores, BFLUTS (except the I-sum), the voiding diary, and Qmax/PVR were not significantly different between groups. Approximately 54% of the alfuzosin group and 62% of the placebo group were satisfied with the treatment. No significant difference was observed between groups according to the presence or grade of BOO.</P><P><B>Conclusions</B></P><P>Alfuzosin might not be more effective than placebo for treating FVD. The presence or the grade of BOO did not affect the results. A further study with sufficient power is needed to determine the efficacy of α1-AR blockers for the treatment of FVD.</P>

Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats

Lee, Kyung-Hoon,Wang, Tao,Jin, Yong-Cheng,Lee, Sang-Bum,Oh, Jin-Ju,Hwang, Jin-Hee,Lim, Ji-Na,Lee, Jae-Sung,Lee, Hong-Gu Korean Society of Animal Sciences and Technology 2014 한국축산학회지 Vol.56 No.2

The aims of study were to investigate the effects of intraperitoneal (i.p.) infusion of ghrelin on pancreatic ${\alpha}$-amylase outputs and the responses of pancreatic proteins to ghrelin that may relate to the pancreatic exocrine. Six male Sprague-Dawley rats (300 g) were randomly divided into two groups, a control group (C, n = 3) and a treatment group (T, $10.0{\mu}g/kg$ BW, n = 3). Blood samples were collected from rat caudal vein once time after one hour injection. The concentrations of plasma ghrelin, cholecystokinin (CCK) and alfa-amylase activity were evaluated by enzyme immunoassay (EIA) kit. Two-dimensional gel electrophoresis (2-DE) analysis was conducted to separate the proteins in pancreas tissue. Results showed that the i.p. infusion of ghrelin at doses of $10.0{\mu}g/kg$ body weight (BW) increased the plasma ghrelin concentrations (p = 0.07) and elevated the plasma CCK level significantly (p < 0.05). Although there was no statistically significant, the ${\alpha}$-amylase activity tended to increase. The proteomics analysis indicated that some pancreatic proteins with various functions were up- or down-regulated compared with control group. In conclusion, ghrelin may have role in the pancreatic exocrine, but the signaling pathway was still not clear. Therefore, much more functional studies focus on these found proteins are needed in the near future.

경북 최초의 신장이식 1례

이중현,김용섭,이정호,이창우,김상욱,이문섭,오민구,하달봉,이경섭 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

신장이식은 환자의 삶의 질을 높이고 거의 정상적인 사회생활을 가능하게 함으로써 말기신부전 환자의 3가지 신대체 요법(혈액투석, 복막투석, 신장이식) 중에 가장 효과적이고 각광받는 치료법이라 할 수 있다. 최근 들어 의료기술과 의학의 발달로 신장이식의 성공률은 과거에 비해 비약적으로 높아지고 있으며 국내에서도 점차 신장이식수술을 받는 환자가 증가하고 있으나 서울이나 다른 대도시에 비해 의료환경이 열악한 중소도시나 지방의 병원에서는 아직도 시행하지 못하고 있는 형편이다. 이에 저자들은 경북 최초로 자체 의료진에 의해 고혈압과 말기 신부전으로 동국대 포항병원에서 혈액투석을 받고 있던 32세의 남자환자에게 부인으로부터 신장을 제공받아 신장이식을 성공적으로 시행하였기에 문헌고찰과 함께 보고하고자 한다. Kidney transplantation is the preferred treatment modality for many patients with end-stage renal disease (ESRD), because it offers improved quality of life over both hemodialysis and peritoneal dialysis. Patients who do well after transplantation generally report improvement in vitality and freedom to return to the style of life that they experienced before their progression to ESRD. Recently, the success rate of kidney transplantation is greatly being increased as the surgical technique and immunosuppressive drugs are developed. Kidney transplantation is also popular in Korea which has good result. But, until now the general hospitals which are located at small city can't try the kidney transplantation because they don't have proper facilities and experts. We firstly experienced a case of kidney transplantation in a 32 years old man with hypertension and chronic renal failure at KyoungBuk province. So we report this case with references.