Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation

Ning Deng,Jun Jian Xiang,Qing Zhang,Sheng Xiong,Wen Yin Chen,Gui Rong Rao,Xun Zhang Wang 한국생활환경학회 2005 BMB Reports Vol.38 No.3

Comments on: a phase 1/2a, dose-escalation, safety and preliminary efficacy study of oral therapeutic vaccine in subjects with cervical intraepithelial neoplasia 3

Ning Zhang,Hanjie Wang,Jili Yang 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.2

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Experimental and numerical analysis of unsteady pressure pulsation in a centrifugal pump with slope volute

Ning Zhang,Minguan Yang,Bo Gao,Zhong Li,Dan Ni 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.10

This study experimentally and numerically investigates the unsteady flow in a centrifugal pump with special slope volute under variousconditions to illustrate the detailed flow structures and pressure pulsation within the model pump. Whole flow passage is consideredduring the numerical simulation; pressure pulsation signals are extracted using nine fast-response pressure transducers. The Root meansquare (RMS) method is introduced to deal with the discrete components at fBPF of the different monitoring points along the volute casing,which is an effective attempt to evaluate the overall pulsating level of the model pump. Results show that numerical method canpredict the components at fBPF effectively; however, it has limited ability in capturing noise frequencies motivated by unsteady separateflow and non-linear interaction effect. Around the nominal flow rate, the predicted amplitudes at fBPF agree well with the experimentalresults, showing larger difference at the off-design conditions. To predict the pulsating level of the components at fBPF, two fitted equationsof the RMS values versus the flow rate and specific speed are carried out, which would be very helpful in evaluating the pressurepulsation level in the centrifugal pump.

Molecular Dynamics Investigation of the Effects of Concentration on Hydrogen Bonding in Aqueous Solutions of Methanol, Ethylene Glycol and Glycerol

Ning Zhang,Weizhong Li,Cong Chen,Jianguo Zuo,Lindong Weng 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

Hydrogen bonding interaction between alcohols and water molecules is an important characteristic in the aqueous solutions of alcohols. In this paper, a series of molecular dynamics simulations have been performed to investigate the aqueous solutions of low molecular weight alcohols (methanol, ethylene glycol and glycerol) at the concentrations covering a broad range from 1 to 90 mol %. The work focuses on studying the effect of the alcohols molecules on the hydrogen bonding of water molecules in binary mixtures. By analyzing the hydrogen bonding ability of the hydroxyl (-OH) groups for the three alcohols, it is found that the hydroxyl group of methanol prefers to form more hydrogen bonds than that of ethylene glycol and glycerol due to the intra-and intermolecular effects. It is also shown that concentration has significant effect on the ability of alcohol molecule to hydrogen bond water molecules. Understanding the hydrogen bonding characteristics of the aqueous solutions is helpful to reveal the cryoprotective mechanisms of methanol, ethylene glycol and glycerol in aqueous solutions.

Pedestrian Choices of Vertical Walking Facilities inside Urban Rail Transit Stations

Ning Zhang,Yunlong Zhang,Xiaojun Zhang 대한토목학회 2015 KSCE JOURNAL OF CIVIL ENGINEERING Vol.19 No.3

This paper investigates pedestrians’ choices between escalator and stairway inside urban rail transit stations during peak hours. After the review of mode choice models, a logit model based on the utility maximization theory is selected. Binary logit models forthe passenger flow distribution are established with walking time, walking distance, gender and age of the passenger as the maininfluencing factors. The influences of these factors are quantitatively analyzed through data collected from three rail transit stationswith different arrangement forms of vertical walking facilities in Nanjing, China. The developed models are verified to produce ahigh accuracy in predicting passengers’ choices of vertical walking facilities. With developed models, sensitivity analyses forselecting escalator for upward and downward pedestrians in the three types of stations are also conducted and compared to furtherreveal the differences in pedestrians' choices of using vertical walking facilities. The modeling procedure and results can provide atheoretical reference for operation decisions, such as the planning of vertical walking facilities and passenger flow organization inbusy rail transit stations.

Numerical method study of how buildings affect the flow characteristics of an urban canopy

Ning Zhang,Weimei Jiang,Fei Hu 한국풍공학회 2004 Wind and Structures, An International Journal (WAS Vol.7 No.3

Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs

Zhang-Yong Ning,Xin-Tao Wu,Yan-Fen Cheng,Wen-Bao Qi,Yu-Fu An,Heng Wang,Gui-Hong Zhang,Shou-Jun Li 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.3

Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date,there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.

Image Reconstruction Model Based on Deep Learning for Defect Detection of Rubber Products

Ning Zhang,권성근 한국멀티미디어학회 2023 멀티미디어학회논문지 Vol.26 No.11

The industrial defect detection method based on deep learning can reduce the cost of traditional manual quality inspection and improve the efficiency of detection. Therefore, it plays an important part in intelligent manufacturing and has gradually become one of the emerging research hotspots in the field of computer vision. It is commonly used in various production and operation and maintenance scenarios such as unmanned quality inspection, intelligent patrol inspection, and quality control. In this paper, according to the detection requirements in reality, a deep image reconstruction network is constructed to realize intelligent quality detection of industrial products. In order to obtain more ideal results, corresponding tests and adjustments are carried out on the constructed network.

piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.

MiRNA320a Inhibitor-Loaded PLGA-PLL-PEG Nanoparticles Contribute to Bone Regeneration in Trauma-Induced Osteonecrosis Model of the Femoral Head

Zhang Ying,Li Chuan,Wei Qiushi,Yuan Qiang,He Wei,Zhang Ning,Dong Yiping,Jing Zhenhao,Zhang Leilei,Wang Haibin,Cao Xiangyang 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH. BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.