http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
강보경(Bo-Kyeong Kang),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박시우(Si-Woo Bark),박원민(Won-Min Pak),김보람(Bo-Ram Kim),안나경(Na-Kyung Ahn),최연욱(Yeon-Uk Choi),배난영(Nan-Young Bae),박지혜(Ji-Hye Park),안동현(Dong-Hyun 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.1
본 연구는 DNCB(2,4-dinitrochlorobenzene) 도포를 통해 아토피 피부염을 유발시킨 BALB/c mice에 참치심장 에탄올 추출물(THEE)을 2주 동안 지속적으로 처리하여 THEE의 아토피 피부염 완화 효과에 대해 연구하였다. 이를 위해 Th1 및 Th2 cytokines의 분비량, 비장 세포 증식능, 혈청 중 총 IgE 함량, 육안 평가 및 skin clinical severity score를 실시하였다. 그 결과 THEE를 지속적으로 도포함으로써 혈청 내 총 IgE의 분비량이 현저히 감소함을 나타내었다. Th1 세포로부터 생성되는 IFN-γ cytokine은 증가하고 염증매개성 cytokine인 TNF-α의 분비량은 감소함을 확인하였다. 또한 Th2 cytokine에 속하는 IL-4, IL-5, IL-13 분비량은 감소하였으며 항염증성 cytokine인 IL-10의 분비량이 증가함을 보여 항아토피 효과를 나타내었다. 육안 평가를 통해서 THEE를 2주간 지속적으로 도포 처리하였을 때 그 증상이 눈에 띄게 완화되는 것을 확인할 수 있었으며, skin clinical severity score에서도 DNCB 단독 처리군과 비교 시 유의적으로 그 값이 감소하였다. 이상의 결과들로부터 THEE는 Th1과 Th2 cytokine의 생성 및 활성을 조절하고 IgE의 과다 발현을 억제함으로써 아토피 피부염의 개선에 뛰어난 효능을 가지고 있는 것으로 사료된다. Atopic dermatitis (AD) is a form of allergic skin inflammatory characterized by late eczematous skin lesions. The incidence of AD is increasing, and it causes problems with administrative costs. Therefore, development of an AD treatment with no side effects is needed. The purpose of this study was to evaluate tuna heart ethanol extract (THEE), a functional extract from by-product of tuna. AD was induced by spreading 2,4-dinitrochlorobenzene (DNCB) on the backside of BALB/c mice. The effect of THEE was tested by measuring skin clinical severity score, secretion of cytokines and IgE, and proliferation. Secretion of TNF-α, IL-4, IL-5, IL-13, and IgE significantly decreased in a THEE-independent manner. In contrast, levels of IL-10 and IFN-γ significantly increased in mice sera and splenocytes. In addition, THEE alleviated AD symptoms compared to the DNCB only group. In conclusion, these results demonstrate that THEE has an inhibitory effect on AD and may be a useful substance for the development of cosmeceuticals.
양파껍질 열수추출물의 in vitro 및 in vivo 항염증 효과
강보경(Bo-Kyeong Kang),김꽃봉우리(Koth-Bong-Woo-Ri Kim),안나경(Na-Kyung Ahn),최연욱(Yeon-Uk Choi),김민지(Min-ji Kim),박시우(Si-Woo Bark),박원민(Won-Min Pak),김보람(Bo-Ram Kim),박지혜(Ji-Hye Park),배난영(Nan-Young Bae),안동현(Dong-Hyun 한국생물공학회 2015 KSBB Journal Vol.30 No.4
Onion (Allium cepa) is one of the flavonoids-rich materials in human diet and onion peel, which is the onion byproducts, contains over 20 times more quercetin than the flesh. In this study, to examine the anti-inflammatory effects of onion peel hot water extract (OPHWE), the cell viability, nitric oxide (NO), pro-inflammatory cytokines, such as interluekin-6 (IL- 6), tumor necrosis factor-α (TNF-α), and IL-1β, were measured using the murine macrophage cell line RAW 264.7 cells. The Balb/c mice were used for an in vivo acute toxicity test and ICR mice were used for measurement of inhibition effects of croton oil-induced mouse ear edema. As a result, NO levels decreased in a dose-dependent manner. The production of IL-6, TNF-α, and IL-1β was suppressed by 38%, 41%, and 34% respectively, compared with that of the LPS only group, without any cytotoxicity. The edema formation in the ICR mouse ear was also reduced compared to that in control. Moreover, there were no mortalities occurred in mice administered 5,000 mg/kg body weight of OPHWE. These results suggest that OPHWE has considerable anti-inflammatory activities and can be regarded as a potent candidate material to treat inflammatory diseases.
참치( Katsuwonus pelamis ) 자숙액 농축물의 마우스 대식세포 및 비장세포에 대한 면역증강활성
강보경 ( Bo Kyeong Kang ),김꽃봉우리 ( Koth Bong Woo Ri ),안나경 ( Na Kyong Ahn ),최연욱 ( Yeon Uk Choi ),김민지 ( Min Ji Kim ),박시우 ( Si Woo Bark ),박원민 ( Bark Won Min ),김보람 ( Bo Ram Kim ),박지혜 ( Ji Hye Park ),배난영 ( Nan 한국수산과학회 2014 한국수산과학회지 Vol.47 No.6
Tuna cooking juice concentrate (TCJC) is by-produced during the canning processing of skipjack tuna Katsuwonus pelamis and it is well known that TCJC contains various nutritional components. Therefore, the immuno-stimulating activity of TCJC was investigated using macrophage RAW 264.7 cell line and the spleen cell isolated from BALB/c mice. The TCJC increased the production of IL-6, TNF-α, and IL-1β in a dose-dependent manner compared to the control in RAW 264.7 cells without any toxicity. In particular, the production of TNF-α was increased over 300-fold. The production of both Th1 cytokine (such as IFN-γ, TNF-α, IL-2, and IL-12) and Th2 cytokine (IL-4, IL-6, IL-10) was also increased by TCJC treatment in splenocytes. Moreover, the TCJC increased the splenocyte proliferation in a concentration-dependent manner compared to control. These results indicate that TCJC may enhance immune function by promoting various cytokine production.
Development of a strategy for the screening of α-glucosidase-producing microorganisms
Bo Zhou,Nan Huang,Wei Zeng,Hao Zhang,Guiguang Chen,Zhiqun Liang 한국미생물학회 2020 The journal of microbiology Vol.58 No.2
α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-D-methylglucoside (AMG) in glass plates was tested for the primary screening of α-glucosidaseproducing strains. First, α-glucosidase-producing Aspergillus niger strains were selected on plates containing EY and AMG based on transparent zone formation resulting from the solubilization of EY by the hydrolyzed product. Conventional methods that use trypan blue (TB) and p-nitrophenyl-α-Dglucopyranoside (pPNP) as indicators were then compared with the new strategy. The results showed that EY-containing plates provide the advantages of low price and higher specificity for the screening of α-glucosidase-producing strains. We then evaluated the correlation between the hydrolytic activity of α-glucosidase and diffusion distance, and found that good linearity could be established within a 6–75 U/ml enzyme concentration range. Finally, the hydrolytic and transglycosylation activities of α-glucosidase obtained from the target isolates were determined by EY plate assay and 3,5- dinitrosalicylic acid-Saccharomyces cerevisiae assay, respectively. The results showed that the diameter of the transparent zone varied among isolates was positively correlated with α-glucosidase hydrolytic activity, while good linearity could also be established between α-glucosidase transglycosylation activity and non-fermentable reducing sugars content. With this strategy, 7 Aspergillus niger mutants with high yield of α-glucosidase from 200 obvious single colonies on the primary screen plate were obtained.
Bandwidth-Related Optimization in High-Speed Frequency Dividers using SiGe Technology
Nan, Chao-Zhou,Yu, Xiao-Peng,Lim, Wei-Meng,Hu, Bo-Yu,Lu, Zheng-Hao,Liu, Yang,Yeo, Kiat-Seng The Institute of Electronics and Information Engin 2012 Journal of semiconductor technology and science Vol.12 No.1
In this paper, the trade-off related to bandwidth of high-speed common-mode logic frequency divider is analyzed in detail. A method to optimize the operating frequency, band-width as well as power consumption is proposed. This method is based on bipolar device characteristics, whereby a negative resistance model can be used to estimate the optimal normalized upper frequency and lower frequency of frequency dividers under different conditions, which is conventionally ignored in literatures. This method provides a simple but efficient procedure in designing high performance frequency dividers for different applications. To verify the proposed method, a static divide-by-2 at millimeter wave ranges is implemented in 180 nm SiGe technology. Measurement results of the divider demonstrate significant improvement in the figure of merit as compared with literatures.
Research on Key Problems of Channel Estimation Based on Plural RBF Neural Network
Nan Wang,Bo Hu,Junyang Zhang,Jinsong Liu,Xinyu Zhang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.10
A new channel estimation method of neural network based on complex radial basis function (CRBF) is proposed to enhance the anti-interference ability of traditional pilot frequency estimation algorithm in power line communication (PLC). This method builds up a new channel model of complex field signals in PLC. The complete response model was established by using transmitting terminal’s pilot signal as input sample data, pilot signal’s frequency response as output sample data, and pre-setting mean square error (MSE) and diffusion constant. Computer simulations show that compared with the traditional algorithm the channel estimation was more accurate and had lower MSE and bit error rate (BER).
( Bo Xu ),( Li Ming Dai ),( Jun Jun Li ),( Meng Deng ),( Hua Biao Miao ),( Jun Pei Zhou ),( Yue Lin Mu ),( Qian Wu ),( Xiang Hua Tang ),( Yun Juan Yang ),( Jun Mei Ding ),( Nan Yu Han ),( Zun Xi Huang 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37°C and could maintain at least 96% activity after being placed at 37°C for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, Km, Vmax, and kcat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.