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Park Yeong-Ju,Hwang Unsik,Park Suyeon,Sim Sol,Jeong Soyeon,Park Misun,Kang Minji,Lee Youngsoo,Song Youngju,Park Hoon,Suh Hee-Jae 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.1
Compound K (CK; 20-O-β-(d-glucopyranosyl)-20(S)-protopanaxadiol) is one of the metabolites of ginsenosides contained in red ginseng (RG) and is known to have high bioavailability. This study aimed to establish the optimal conditions for enzyme treatment to convert ginsenosides from RG extract to CK, and to prove the characteristics of bioconverted red ginseng (BRG) extract. CK was not detected in unenzyme-treated RG extract, and in the single-step enzyme treatment, it was produced at less than 4.58 mg/g only in treatment group with Pyr-flo or Sumizyme AC (at 50 °C for 48 h). The highest yield of CK (14.32 mg/g) was obtained by Ultimase MFC treatment at 50 °C for 48 h after treatment with a mixture of Pyr-flo and Rapidase at 50 °C for 24 h. Total polyphenol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) (ABTS) radical scavenging activity were higher in BRG than in RG (p < 0.5). High-fat diet (HD) rat fed 1% BRG had significantly lower body weight, heart weight, fat pads (periosteal fat, epididymal fat), serum glucose levels, and hepatic triglyceride levels than those HD rat fed 1% RG (p < 0.05). In conclusion, the sequential enzymatic bioconversion was produces higher CK in RG root extract than single-step enzyme treatment.
Doping effects of multiferroic manganitesYMn0.9X0.1O3(X=Al, Ru, and Zn)
Park, Junghwan,Kang, Misun,Kim, Jiyeon,Lee, Seongsu,Jang, Kwang-Hyun,Pirogov, A.,Park, J.-G.,Lee, Changhee,Park, S.-H.,Kim, Hyoung Chan American Physical Society 2009 Physical review. B, Condensed matter and materials Vol.79 No.6
Park, Min Hee,Lee, Misun,Nam, Geewoo,Kim, Mingeun,Kang, Juhye,Choi, Byung Jo,Jeong, Min Seock,Park, Kang Ho,Han, Wan Hui,Tak, Eunyoung,Kim, Min Sun,Lee, Juri,Lin, Yuxi,Lee, Young-Ho,Song, Im-Sook,Choi National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.47
<P><B>Significance</B></P><P>Microglial dysfunction accompanying the loss of phagocytic ability and the overexpression of neurotoxic factors presents a positive-feedback loop that contributes to the rapid progression of neurodegeneration. Termination of this cycle is considered a promising strategy to halt the progression of neurodegenerative diseases, including Alzheimer’s disease; however, effective chemical reagents for this purpose have been very limited. Herein, we report a compact synthetic molecule capable of restoring microglial dysfunction and improving cognitive function. Our in-depth studies of such a molecular entity could be beneficial toward the urgent global search for a new and effective treatment of neurodegenerative disorders.</P><P>As a central feature of neuroinflammation, microglial dysfunction has been increasingly considered a causative factor of neurodegeneration implicating an intertwined pathology with amyloidogenic proteins. Herein, we report the smallest synthetic molecule (<I>N</I>,<I>N</I>′-diacetyl-<I>p</I>-phenylenediamine [DAPPD]), simply composed of a benzene ring with 2 acetamide groups at the <I>para</I> position, known to date as a chemical reagent that is able to promote the phagocytic aptitude of microglia and subsequently ameliorate cognitive defects. Based on our mechanistic investigations in vitro and in vivo, 1) the capability of DAPPD to restore microglial phagocytosis is responsible for diminishing the accumulation of amyloid-β (Aβ) species and significantly improving cognitive function in the brains of 2 types of Alzheimer’s disease (AD) transgenic mice, and 2) the rectification of microglial function by DAPPD is a result of its ability to suppress the expression of NLRP3 inflammasome-associated proteins through its impact on the NF-κB pathway. Overall, our in vitro and in vivo investigations on efficacies and molecular-level mechanisms demonstrate the ability of DAPPD to regulate microglial function, suppress neuroinflammation, foster cerebral Aβ clearance, and attenuate cognitive deficits in AD transgenic mouse models. Discovery of such antineuroinflammatory compounds signifies the potential in discovering effective therapeutic molecules against AD-associated neurodegeneration.</P>
Cardiac Regeneration with Human Pluripotent Stem Cell-Derived Cardiomyocytes
Park, Misun,Yoon, Young-sup The Korean Society of Cardiology 2018 Korean Circulation Journal Vol.48 No.11
<P>Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), which are collectively called pluripotent stem cells (PSCs), have emerged as a promising source for regenerative medicine. Particularly, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown robust potential for regenerating injured heart. Over the past two decades, protocols to differentiate hPSCs into CMs at high efficiency have been developed, opening the door for clinical application. Studies further demonstrated therapeutic effects of hPSC-CMs in small and large animal models and the underlying mechanisms of cardiac repair. However, gaps remain in explanations of the therapeutic effects of engrafted hPSC-CMs. In addition, bioengineering technologies improved survival and therapeutic effects of hPSC-CMs in vivo. While most of the original concerns associated with the use of hPSCs have been addressed, several issues remain to be resolved such as immaturity of transplanted cells, lack of electrical integration leading to arrhythmogenic risk, and tumorigenicity. Cell therapy with hPSC-CMs has shown great potential for biological therapy of injured heart; however, more studies are needed to ensure the therapeutic effects, underlying mechanisms, and safety, before this technology can be applied clinically.</P>
( Misun Yun ),( Hee Eun Jo ),( Namhee Kim ),( Hyo Kyeong Park ),( Young Seo Jang ),( Ga Hee Choi ),( Ha Eun Jo ),( Jeong Hyun Seo ),( Ji Ye Mok ),( Sang Min Park ),( Hak-jong Choi ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.4
Excessive alcohol consumption can have serious negative consequences on health, including addiction, liver damage, and other long-term effects. The causes of hangovers include dehydration, alcohol and alcohol metabolite toxicity, and nutrient deficiency due to absorption disorders. Additionally, alcohol consumption can slow reaction times, making it more difficult to rapidly respond to situations that require quick thinking. Exposure to a large amount of ethanol can also negatively affect a person’s righting reflex and balance. In this study, we evaluated the potential of lactic acid bacteria (LAB) to alleviate alcohol-induced effects and behavioral responses. Two LAB strains isolated from kimchi, Levilactobacillus brevis WiKim0168 and Leuconostoc mesenteroides WiKim0172, were selected for their ethanol tolerance and potential to alleviate hangover symptoms. Enzyme activity assays for alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) were then conducted to evaluate the role of these bacteria in alcohol metabolism. Through in vitro and in vivo studies, these strains were assessed for their ability to reduce blood alcohol concentrations and protect against alcohol-induced liver damage. The results indicated that these LAB strains possess significant ethanol tolerance and elevate ADH and ALDH activities. LAB administration remarkably reduced blood alcohol levels in rats after excessive alcohol consumption. Moreover, the LAB strains showed hepatoprotective effects and enhanced behavioral outcomes, highlighting their potential as probiotics for counteracting the adverse effects of alcohol consumption. These findings support the development of functional foods incorporating LAB strains that can mediate behavioral improvements following alcohol intake.