New Insight for Fluoroquinophenoxazine Derivatives as Possibly New Potent Topoisomerase Ⅰ Inhibitor

Kang, Da-Hye,Kim, Jung-Sook,Jung, Mi-Ja,Lee, Eung-Seok,Jahng, Yurngdong,Kwon, Youngjoo,Na, Younghwa 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.18

Fluoroquinolones, represented by ciproxacin and norfloxacin, are well known clinical antimicrobial agents, and their phenyl ring expanded quinophenoxazines are reported as possible antitumor active compounds. These quinophenoxazines are known to inhibit DNA topoisomerase II essential for cell replication cycle. But there were no reports for topoisomerase I inhibition study for these compounds. In this report, we have prepared a few quinophenoxazine analogues and tested their topoisomerases I and II inhibitory activities and cytotoxicity. From the result, we found that qumophenoxazine analogues possessed strong topoisomerase I inhibitory capacity as well as topoisomerase II inhibition. Among the compounds prepared, A-62176 analogues showed strong topoisomerases I and II inhibitory activities. Interestingly, compound 8 missing the 3-aminopyrrolidine moiety at C2 position has similar potent inhibitory capacity against topoisomerases I & II at higher concentrations (20 and 10 uM, respectively). But compound 8 inhibited topoisomerase I function more selectively at lower concentration, 2 uM. Our observation mi&ht strongly implicate that fluoroquinophenoxazines can be developed as efficient topoisomerase I inhibitor with the elaborate modification.

비결핵성 마이코박테리아 폐질환의 임상경과

최강현, 한혜숙, 정혜원, 안진영, 이기만, 김미경 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

-본문참고-

사회봉사와 전공학문을 연계한 봉사학습에 관한 연구 : 문헌정보학을 중심으로 With a Special Reference in Library and Information Science

강미혜 德成女子大學校 1997 德成女大論文集 Vol.28 No.-

The social service is not simply an important altruistic activity or an obligatory exercise, but an essential element of the university activity. In this study, the literature of the social service is reviewed. And then, various programs of integrating social service with academic study are presented and the generally accepted principles of good practice are discussed. Finally the service programs in general libraries are explored that the library and information science can utilize.

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

공동주택 사전성능평가를 위한 성능평가 시스템 개발에 관한 연구

강미선,이상현,김하나,엄혜진 대한건축학회 2003 대한건축학회논문집 Vol.19 No.10

The purpose of this study is to propose an evaluation framework for Collective Housing and to develop the Design Evaluation System. The Performance Evaluation System of Collective Housing in the Design Development Stage evaluates its design results by running CAD program. The results are automatically reflected in the design process. The scope of this study will be limited to the contents within the Barrier Free Design. Running the design evaluation program requires the conversion of design forms and design checklist to computerized ones. To efficiently conduct this process, First, organize the existing guideline of the Barrier Free Design. Second, define attributes of each part in Building Data Model with the analysis of the checklist. Third, develop the Performance Analysis agent that compares Building Data Model with translated checklists. Finally, verify the efficiency of the Performance Evaluation System by seeking experts' advice on the result of the program.

전국 단위 감시체계의 현황과 전망

강성규,안연순,지영구,김형옥,하미나,권호장,백남종,김성아,홍윤철,김재용,강대희,정혜선,하은희 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

일부 흡연 대학생의 지각된 스트레스와 흡연실태에 대한 상관관계 연구

강초록,권시내,권혜림,박수경,송인혜,오해인,이미지,한승아,황선아,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

In spite of the seriousness of smoking harm has been developed these days. the increase of national smoking rate and that the people in the twenties are especially leading the rise of national smoking rate is an important issue of national health. Smoking of the university students can occur health problem and cause loss of manpower and expenses in their social activity period. So then the actual state for prevention of smoking and development of a program to prohibit smoking has to be seized urgently and starting with the study of the realities of university students, the effort to find a related fluent of smoking has to be studied. Related to this, the purpose of this study is to seize the general characteristics of smoking university students and to find out the relationship between perceived stress and smoking status among smoking university students. In addition, by collating the relationship between perceived stress and smoking status among smoking university students we have the purpose to furnish the basal data of stress care, the best way to cope with stress through group training and the plan for nursing implementation to decrease the smoking rate. For this study, a survey was conducted for 204 smoking university students of 13 universities in Seoul from 9th of May to 16th of May, 2003. The collected data was analyzed by t-test, Pearson Correlation and ANOVA in the statistical packages SPSS/PC. Major findings of this study are as follows; 1. Of the 204 respondents 85.4% of them were male and 14.6% of them were female. The average age of the sample was 23.19 years old and 41.8% of them were in senior, 21.4% in junior, 16.9% in sophomore, 19.9% in freshman grade. The major motivation to start smoking was 'because of curiosity'(33.8%) and the first smoking experience was at 'senior high school' (41.9%) and at 'college' (41.9%). 2 The highest scored stress factor of smoking university students were stresses about school work. 3. 72.7% had experience to quit smoking at least one or more times. Most of the reason to keep on smoking was 'short will to quit'(51.0%) and the smoking place was usually at 'school' (41.4%). Most respondents for the number ofsmoking friends were '5 people and above' (73.2%). 4. There was a negative relationship between the general characteristics and the entire perceived stress, however, there was a positive relationship between the general characteristics and the perceived stress in 4 categories which is 'sex distinction', 'major of study', 'residence form' and' inmate'. 5. There was a negative relationship between the general characteristics and the entire smoking status, however, there was a positive relationship between the general characteristics and the smoking status in 2 categories which is 'presence of hobby' and 'number of smoking friends'. 6. There was a negative relationship between the perceived stress and the smoking status. Through this study we have seized the relationship between the general characteristics, perceived stress and the smoking status of smoking university students. There was a positive relationship between some of the general characteristics with the perceived stress and the smoking status of smoking university students. Also there was a negative relationship between the perceived stress and the smoking status.

Capsaicin에 의한 간암세포주의 Apoptosis 유도

정미영,강혜정,문애리 德成女子大學校 藥學硏究所 2000 藥學論文誌 Vol.11 No.1

Hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. In the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in SK-Hep-1 hepatocellular carcinoma cells. Treatment of capsaicin inhibited growth of SK-Hep-1 cells in a concentration-dependent manner. The inhibitory effect of capsaicin on SK-Hep-1 cell growth was mainly due to the induction of apoptosis as evidenced by DNA fragmentation and nuclear condensation. Furthermore, capsaicin prominently reduced the ratio of anti-apoptotic Bcl-2 to proapoptotic Bax. We also show that the activity of DEVDase (caspase-3 like) is more likely involved than that of YVADase (caspase-1 like) in capsaicin-induced growth inhibition. These results deminstrate that capsaicin efficiently induced apoptosis in SK-Hep-1 cells through a caspase-3-dependent mechanism, which may contribute to the chemopreventive function for hepatocellular carcinoma.

영아자(Phyteuma japonicum Miq.)의 성분 조성

정미자,신정혜,이수정,홍성국,강호중,성낙주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

산채식물인 영아자(Horned Rampion ; Phyteuma japonicum Miq.)의 영양학적인 가치를 평가코져 야생 및 평지재배 시료를 잎과 줄기로 구분하여 일반성분, 비타민 C, 유리당, 무기물, 핵산관련물질, 구성 아미노산 및 유리아미노산을 분석하였다. 야생과 재배 영아자의 회분은 1.2∼2.7%의 범위였고, 조지방과 조단백질은 재배시료가 조섬유는 야생시료에서 더 높은 함량으로 정량되었고, 전당은 두 시료간에 대차를 보이지 않았다. 비타민 C는 줄기보다는 잎에서 재배시료보다는 야생시료에서 더 높게 정량되었다. 유리당은 야생 및 재배시료 모두 glucose, frucose 및 sucrose가 잎보다 줄기에서 높게 정량되었다. 무기물은 총 9종이 분석되었는데 이중 칼슘의 함량이 가장 높아 재배시료의 경우 잎은 34374.0㎎/㎏, 즐기는 9584.1㎎/㎏였고, 그 다음으로 칼륨, 마그네슘의 순으로 많았으며, 야생시료도 비슷한 경향을 보였다. 핵산 관련물질은 CMP, UMP, IMP, AMP 및 hypoxanthine이 동정되었는데 잎과 줄기 모두 야생시료에서는 hypoxanthine이, 재배시료에서는 AMP가 월등히 높게 정량되었다. 구성아미노산은 총 17종으로 야생시료에서는 glutamic acid, 재배시료는 잎의 경우 aspartic acid, 줄기의 경우 glutamic acid의 함량이 가장 많았다. 유리아미노산은 총 29종이 동정되었고 야생시료의 잎에서는 glutamic acid, 줄기에서는 γ-aminoisobutyric acid, 재배시료의 줄기에서는 asparagine이 가장 높은 함량으로 정량되었다. This research is to establish the basic data of the nutritive value and improve our diet. In the part of th leaf and stem of the wild cultivated horned rampion(Phyteuma japonicum Mig), the components such as chemical composition, vitamin C, free sugar, mineral, nucleotide and its related compounds, composition and free amino acid were analyzed one after another. Content of the crude lipids and proteins was determined much higher in its wildness than in its cultivated horned rampion ; while, that of carbohydrates was higher in the former than in the latter. The content of vitamin C was retained higher in the leaf than in the stem horned rampion. And the content of calcium among the detected minerals was outstanding in all of the samples collected, and potassium and magnesium was the next ones in its order. The main components of free sugars in both the wild and cultivated horned rampion were glucose and fructose, and their content was higher in the stem than in the leaf. Nucleotide and its related compounds were identified with 5 kinds of nuciotides such as CMP, UMP, IMP, AMP and hypoxanthine(Hx), and the content of Hx and AMP was the highest in the wild and cultivated samples, respcetively. In the composition amino acid of the wild horned rampion, glutamic acid, aspartic acid and phenylalanine was outstandingly abundant; while, such amino acid as methionine and proline was small and besides cysteine couldn't be detected in the stem. Total amounts of composition amino acid in the leaf was 2118.0 and 1120.1㎎% in the wild and cultivated sample, respectively. In the free amino acid of horned rampion, the total amount ranged from 8.5 to 50.1㎎%, which were lower level than that of composition amino acid. But the number of free amino acid was 29 kinds, which was bigger in its number than of composition amino acid detected 17 kinds.

분사처리 후 산부식 표면처리된 교정용 미니 임플랜트의 골유착능에 관한 연구

전미선,강윤구,모성서,이근혜,국윤아,김성훈 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.5

본 연구에서는 교정 치료 시 골내 고정원으로 사용되는 교정용 임플랜트의 표면처리 여부가 골유착능에 있어서 어떠한 효과를 보이는지 제거회전력의 측정을 통해 알아보고자 하였으며, 그에 따른 교정력 적용의 확장과 임상적 의의를 알아보고자 하였다. 실험군은 분사처리 후 산부식(Sand-blasted Large grit, and Acid etched, SLA) 표면 처리된 교정용 미니 임플랜트인 C-implant (Cimplant, Seoul, Korea)를 사용하였으며 대조군은 같은 디자인이지만 표면 처리를 하지 않은 평활면 C-implant를 사용하였다. 실험군과 대조군을 각각 2개씩 11마리의 가토 경골에 식립하였고 식립 후 6주에 가토를 희생시켜 제거회전력을 측정하여 t-test를 통하여 두 군의 제거회전력 차이의 통계적 유의성을 알아보았으며 조직표본을 만들어 조직소견을 관찰하였다. 실험결과 제거회전력은 SLA 처리한 C-implant 군이 평활면 C-implant 군보다 통계적으로 유의성 있게 높은 결과를 보였다 (p < 0.05). 평활면 C-implant 군의 평균 제거회전력 값은 4.614 Ncm이고, SLA C-implant 군의 평균 제거회전력 값은 6.286 Ncm로, SLA 군이 평활면 군보다 73% 더 높은 제거회전력에 대한 저항성을 나타내었다. 이상의 연구 결과에서 SLA 표면처리가 C-implant의 골유착능을 증가 시켰음을 알 수 있었다. 따라서 표면 처리된 교정용 미니 임플랜트는 기존의 임플랜트에 비해 좀 더 강한 힘에 저항할 수 있으며 탈락률을 낮출 수 있을 것으로 생각된다. Objective: The purpose of this study was to compare the torque resistance to removal of sandblasted large grit and acid etched (SLA) surface treated orthodontic mini-implants and smooth surface orthodontic mini-implants as well as performing histologic observations. Methods: Two groups of custom screw shaped orthodontic mini-implants (C-implant, 1.8 mm outer diameter × 9.5 mm length, Cimplant, Seoul, Korea) were designated. 22 SLA treated C-implants (SLA group) and 22 machined surface C-implants (machined group) were placed in the tibia metaphysis of 11 adult New Zealand white rabbits. Following a 6-week healing period, the rabbits were sacrificed. Subsequently, the C-implants were removed under reverse torque rotation with a digital torque measuring device and independent t-test was performed. Selected tissues were prepared for histologic observation. Results: The SLA group presented a higher mean removal torque value (6.286 Ncm) than the machined group (4.491 Ncm) which was statistically significant (p < 0.005). Histologic observation revealed a trend of more new bone formation in contact with the screw surface in the SLA group than the smooth group. Conclusions: The results of this study suggested that SLA surface treatment can enhance the osseintegration potential for C-orthodontic mini-implants.