결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

지속성 외래복막투석 환자들의 예후에 영향을 미치는 요인들에 관한 고찰

권혁만,김찬덕,조지형,장민화,최혁준,이현철,박자용,박선희,김용림 대한신장학회 2004 대한신장학회잡지 Vol.23 No.2

Intrauterine tamponade using Bakri balloon catheter during cesarean section for placenta previa

( Seon Hwa Lim ),( Jung Hwa Park ),( Ga Hyun Son ),( Baik Lee ),( Hee Young Cho ),( Young Han Kim ),( Yong Won Park ),( Ja Young Kwon ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

Since 2009, our institute has been applying Bakri balloon for intrauterine balloon tamponade as a first choice to reduce undesired hysterectomy for postpartum bleeding. The aim of this study was to analyze the efficacy of intrauterine tamponade using Bakri balloon catheter during cesarean section for abnormal placentation. Retrospective review of the medical records of patients who had underwent elective cesarean section for abnormal placentation between Aug 2009 and July 2010 was performed. The surgery and insertion of the Bakri balloon catheter were performed by a single qualified operator (JYK). Bakri balloon was applied when a bleeding over 500 mL and tachycardia were noted during the surgery. A total of 72 patients (low-lying 18, previa partialis 10, previa totalis 44) underwent cesarean section for abnormal placentation during the study period. Forty patients (55.6 %) required intrauterine balloon tamponade during cesarean section due to postpartum bleeding from the low uterine segment, of which 72.7 % of cases was placenta previa totalis). And immediate hysterectomy was performed in 2 patients (2.8%) due to profuse bleeding. Of those who received the Bakri balloon uterine tamponade, 30.2 % (13/40) required additional treatment such as bilateral uterine artery embolization (11/40) or hysterectomy (2/40) to achieve complete bleeding control. No material mortality or balloon related complication was encountered. Intrauterine tamponade using Bakri balloon was efficient in managing postpartum bleeding following cesarean section for abnormal placentation to avoid hysterectomy.

재활전문 간호사 실무 표준 개발을 위한 기초 조사

강현숙(Kang Hyun Sook),임난영(Lim Nan Young),서문자(Suh Moon Ja),이명화(Lee Myung Hwa),김정화(Kim Jeong Hwa),서연옥(Suh Yeon Ok),조복희(Cho Bok Hee),김인자(Kim In Ja),송충숙(Lee Jeong Ja),박인주(Song Chung Sook),이성숙(Park In Joo) 한국재활간호학회 2002 재활간호학회지 Vol.5 No.2

The purpose of this study is to develop the standards of care in rehabilitation nurse specialist.<br/> This study was a descriptive survey.<br/> The data were collected 143 nurses who were worked in rehabilitation unit at 4 university hospital and 3 rehabilitation center in Korea from Aug. to Nov. 2000.<br/> The questionnaire was consisted of 78 items developed by the standards of care in rehabilitation nurse specialist in A.R.N. and the practice contents of care at rehabilitation unit in Korea.<br/> Collected data were analyzed by frequency, percentage, mean, S.D. ANOVA<br/> The results are as follows<br/> 1. For the adequency of the practice contents of rehabilitation nursing, area of skin care is the highest score, and safety, elimination, emotion, respiration, was ordered.<br/> 2. The adequency of the practice contents of rehabilitation nursing according to age, educational level. position. clinical experience, clinical experience in rehabilitation unit were significant difference.<br/> <br/> <br/> <br/>

재활 전문간호사 교육과정안 개발연구

강현숙(Kang Hyun Sook),서연옥(Suh Yeon Ok),이명화(Lee Myung Hwa),김정화(Kim Jeong Hwa),임난영(Lim Nan Young),서문자(Suh Moon Ja),이정자(Lee Jeong Ja),박인주(Park In Joo),조복희(Cho Bok Hee),김인자(Kim In Ja),소희영(So Hee Young),송충숙( 한국재활간호학회 2001 재활간호학회지 Vol.4 No.2

The purpose of this study is to develop a systematic and efficient curriculum for the rehabilitation nurse specialist program.<br/> This research was carried out as a group work of 15 experts in order to share vanous opinions about the curriculum. and also through literature review. Articles. curriculums of other Clinical Nurse Specialist Programs. medical laws guidelines. as well as Clinical Nurse Specialist Program from the Korean Nurses Association were reviewed, and the issue was discussed throughly via group meetings.<br/> <br/> The developed cunicul urn is as follows:<br/> 1. Educational philosophy lies in the fact that the rehabilitation nurses support the patients to maximize their potential and functional level. so that they could maintain healthy state and re-adapt to changed environment. Furthermore the rehabilitation nurses are disposed of arbitrary decision power under their own responsibility. thus they take charge of we]fare and healthy environment of the local society through the patients (subjects) and local resources.<br/> 2. Educational goals are to train rehabilitation nurse specialist. who correspond to the social needs. so to say. those who have the know]edge and skills for nursing practice, education and research.<br/> 3. The curriculum consists of 37 credits, of which 24 credits are based on lectures and 13 credits based on clinical practice.<br/> General courses are 3 subjects (5 credits): nursing theory, nursing research. and laws/ethics, Mandatory courses are 8 subjects with 19 credits: advanced physical assessment, pharmacology, pathophysiology, issues in rehabilitation nursing, advanced rehabilitation nursing intervention Ⅰ. advanced rehabilitation nursing intervention Ⅱ. sports physiology. special rehabilitation nursing intervention.<br/> As for the clinical practice courses, assesment and evaluation for rehabilitation (64 hours), community and home based rehabilitation nursing(128 hours), hospital based rehabilitation nursing(l28 hours), institution based rehabilitation nursing(96 hours) would be treated.<br/> 4. Contents of the courses were developed to correspond with the courses' objectives and specific items.<br/> 5. Evaluation would be carried out both in the lecure and in the clinical practice. The knowledge and skills of the students would be measured to ensure full validity and credibility.<br/> However this developed curriculum should be continuously modified and updated in more desirable direction.<br/> <br/> <br/>

Heavy Metal Concentrations in the Fruticose Lichen Usnea aurantiacoatra from King George Island, South Shetland Islands, West Antarctica

Lim, Hyoun-Soo,Han, Myung-Ja,Seo, Dong-Cheol,Kim, Ji-Hee,Lee, Jae-Il,Park, Hyun,Hur, Jae-Seoun,Cheong, Yong-Hwa,Heo, Jong-Soo,Yoon, Ho-Il,Cho, Ju-Sik The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5

The accumulation of selected heavy metals in the fruticose lichen Usnea aurantiacoatra is reported in the vicinity of the Korean research station on Barton Peninsula, King George Island, West Antarctica. To assess the impact of human activities in the study area, all samples were divided into five groups according to distance from the research station. The corresponding heavy metal levels in samples near the station were relatively higher than those collected far from the station. In particular, a very high level of Pb near the station strongly suggests the anthropogenic release of this pollutant. The relationship between trace metal content and age of the lichen was investigated, but no significant difference was found. When evaluating the vertical distribution of heavy metals in lichen thalli, most elements, particularly Pb, accumulated preferentially in the upper parts, probably due to the morphology of U. aurantiacoatra. Therefore, the vertical distribution of heavy metals in fruticose lichens should be considered to enhance data quality in biomonitoring studies.

Editing of MSH4 gene related to sporulation of Pleurotus pulmonarius by CRISPR-Cas9 system

Ja-Yoon Kim,Jong-In Choi,Seon-Hwa Lim,Da-Bin Kim,Hee-Wan Kang 한국버섯학회 2020 버섯 Vol.24 No.1

Fluoxetine affords robust neuroprotection in the postischemic brain via its anti-inflammatory effect

Lim, Chae-Moon,Kim, Seung-Woo,Park, Ju-Young,Kim, Chaekyun,Yoon, Sung Hwa,Lee, Ja-Kyeong Wiley Subscription Services, Inc., A Wiley Company 2009 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.87 No.4

<P>Fluoxetine is a selective serotonin reuptake inhibitor that is widely used in the treatment of major depression including after stroke. In this study, we tested whether fluoxetine protects neuronal death in a rat cerebral ischemia model of middle cerebral artery occlusion (MCAO). The administration of fluoxetine intravenously (10 mg/kg) at 30 min, 3 hr, or 6 hr after MCAO reduced infarct volumes to 21.2 ± 6.7%, 14.5 ± 3.0%, and 22.8 ± 2.9%, respectively, of that of the untreated control. Moreover, the neuroprotective effect of fluoxetine was evident when it was administered as late as 9 hr after MCAO/reperfusion. These neuroprotective effects were accompanied by improvement of motor impairment and neurological deficits. The fluoxetine-treated brain was found to show marked repressions of microglia activation, neutrophil infiltration, and proinflammatory marker expressions. Moreover, fluoxetine suppressed NF-κB activity dose-dependently in the postischemic brain and also in lipopolysaccharide-treated primary microglia and neutrophil cultures, suggesting that NF-κB activity inhibition explains in part its anti-inflammatory effect. These results demonstrate that curative treatment of fluoxetine affords strong protection against delayed cerebral ischemic injury, and that these neuroprotective effects might be associated with its anti-inflammatory effects. © 2008 Wiley-Liss, Inc.</P>

활성탄 재질과 사용연수에 따른 Geosmin과 MIB 흡착특성

이화자(Hwa Ja Lee),손희종(Hee Jong Son),이철우(Chul Woo Lee),배상대(Sang Dae Bae),강임석(Lim Seok Kang) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.4

활성탄 재질별 geosmin과 2-MIB의 최대 흡착량은 석탄계 재질의 활성탄이 가장 우수한 것으로 나타났고, 다음으로 야자계, 목탄계 활성탄 순으로 나타났으며, geosmin과 2-MIB에 대한 석탄계 활성탄의 최대 흡착량(X/M)은 신탄의 경우 야자계와 목탄계 활성탄에 비해 각각 1.2~1.9배 및 2.1~2.6배 정도 높은 것으로 조사되었다. 또한, 3.1년 사용탄의 경우는 석탄계와 목탄계 재질의 활성탄에서 높게 나타났으며, 5.9년 사용탄의 경우는 목탄계 재질의 활성탄이 석탄계 재질의 활성탄보다도 높게 나타났다. 활성탄에서의 흡착용량을 나타내는 k값의 경우 활성탄 재질별, 사용연수별 geosmin과 2-MIB에 대해 전체적으로 geosmin이 크게 나타나고 있어 활성탄 흡착공정에서 2-MIB 보다 제거가 용이한 것으로 조사되었다. 활성탄 사용율(CUR)은 석탄계 재질의 활성탄이 geosmin과 2-MIB에 대해 1.72 g/day 및 1.44 g/day, 야자계나 목탄계 활성탄의 경우는 각각 1.72와 2.05 g/day 및 2.12와 1.90 g/day의 활성탄을 사용하여야만 제어가 가능한 것으로 조사되었으며, 또한, 3.1년과 5.9년 사용탄의 경우는 목탄계 재질의 활성탄이 geosmin과 2-MIB에 대해 각각 3.13과 4.57 g/day 및 2.87과 4.14 g/day로 나타나 다른 재질의 활성탄들에 비해 적은 양으로도 geosmin과 2-MIB를 제어할 수 있는 것으로 나타났다. 석탄계와 야자계 재질의 신탄, 3.1년 및 5.9년 사용탄들에 대해 geosmin과 2-MIB의 최대 흡착량(ng/g)과 비표면적(m2/g) 및 총 세공용적(cm3/g)에 대한 상관성 조사결과, 최대 흡착량은 비표면적 보다는 총 세공용적이 높은 상관성을 가지는 것으로 나타났으며, 최대 흡착량과 총 세공용적의 상관식은 geosmin의 경우는 y = 264,459 × -79,047(R² = 0.95), 2-MIB는 y = 319,650 × -101,762(R² = 0.93)으로 나타났다. Adsorption performance of odorous compounds such as geosmin and 2-MIB on granular activated carbon were evaluated in this study. The coal-based activated carbon was found more effective than other carbons in adsorption of geosmin and 2-MIB. The wood-based virgin activated carbon was less effective than coconut- and coal-based carbon in adsorption nevertheless having larger pore volume and specific surface area than others carbons. The maximum adsorption capacity(X/M) of coal-based activated carbon for geosmin and 2-MIB was 1.2~1.9 and 2.1~2.6 times larger than coconut- and wood-based virgin activated carbon, respectively. Carbon usage rate (CUR) of coal-, coconut- and wood-based virgin activated carbons for geosmin and 2-MIB were 1.72 and 1.44 g/day, 1.72 and 2.05 g/day and 2.12 and 1.90 g/day, respectively. In the evaluation of adsorption isotherm of geosmin and 2-MIB for coal-, coconut- and wood-based virgin activated carbons, k value of 2-MIB was lower than geosmin, It menas 2-MIB is more difficult to remove by activated carbon adsorption than geosmin. The relationship of max. adsorption versus total pore volume of coconut- and wood-based virgin and used activated carbon for geosmin and 2-MIB were y = 264,459 × -79,047(R² = 0.95), y = 319,650 × -101,762(R² = 0.93).

오존과 과산화수소를 이용한 Geosmin과 2-MIB 산화

이화자(Hwa Ja Lee),손희종(Hee Jong Son),노재순(Jae Soon Roh),이상원(Sang Won Lee),지기원(Ki Won Ji),유평종(Pyung Jong Yoo),강임석(Lim Seok Kang) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.7

수돗물내의 불쾌한 이취미는 수도사업자에게 여러 가지 문제를 일으킨다. 비록 이취미는 건강상에 유해하지는 않지만 소비자들에게 수돗물의 안정성을 의심하게 되는 주된 요인이 되고 있다. 본 연구에서는 낙동강 원수와 급속 모래여과 처리수에 함유된 geosmin에 대한 오존과 오존/과산화수소 공정에서 접촉시간별 제거경향을 조사한 결과, 오존 단독공정에 비하여 오존과 과산화수소 혼합공정이 오존 단독공정 보다 접촉시간별로 geosmin 제거율이 월등히 증가하였다. 오존과 과산화수소 투입농도에 따른 여과수 중의 geosmin과 2-MIB의 제거특성 평가에서 2-MIB 보다 geosmin이 오존 및 오존/과산화수소 공정에서 제거가 용이한 것으로 나타났다. 원수 및 여과수에 함유된 geosmin과 여과수에 함유된 2-MIB에 대해 오존 주입농도 0.5∼2.0 mg/L 범위에서 오존과 과산화수소 주입비율 (H₂O₂/O₃)에 따른 각각의 반응 속도상수 k의 변화를 조사한 결과, 오존 및 오존과 과산화수소의 주입비율이 증가할수록 반응 속도상수 k가 급격히 증가하였으며, 오존과 과산화수소의 주입비율이 어느 한계 이상에 도달하면 반응 속도상수 k는 더 이상 증가하지 않는 것으로 나타났다. 따라서 오존 주입농도 0.5∼2.0 mg/L 범위에서 오존 대비 과산화수소의 적정 주입비율(H₂O₂/O₃) 1∼2 사이인 것으로 나타났다. 급속 모래여과 처리수에서 보다는 오존과 OH 라디칼 소비물질이 많이 존재하는 원수에서의 반응속도 상수가 더 낮은 것으로 나타났다. 오존 주입농도별로 과산화수소 주입농도에 대한 급속 모래여과 처리수중의 geosmin이 제거되는 반감기를 조사한 결과, 오존과 과산화수소 주입농도가 증가할수록 geosmin의 반감기는 급격히 줄어들었으며, 오존만 2 mg/L 주입하여 geosmin을 산화시킨 경우보다 오존 2 mg/L와 과산화수소 10 mg/L를 함께 주입한 경우 반감기가 38.9분에서 4.6분으로 8.5배 정도 감소되는 것으로 나타났다. Unpleasant tastes and odors in drinking water cause same problems for water utilities across Korea. Even though tastes and odors do not create health problems, they are main concerns for consumers who determine the safety of their drinking water. In this study, two different odor producing compounds(geosmin, 2-MIB) in the Nakdong river water and rapid sand filtered water were treated by advanced oxidation of O₃/H₂O₂ process. The experimental results showed that the removal efficiency of geosmin with the use of 5 mg/L of O₃ and H₂O₂ was higher than efficiency with the use of O₃ alone for both the raw water and the sand filtered water. And in general, the removal efficiency of geosmin was higher than 2-MIB in the sand filtered water. Under the range of O₃ concentration 0.5∼2.0 mg/L, the removal rate constants(k) of geosmin for the raw and sand filtered waters, and the one of 2-MIB in the sand filtered water were increased rapidly as doses of O₃ and H₂O₂/O₃ increased. The removal rate constants(k) do not increase any more when H₂O₂/O₃ ratio increases above the optimum ratio. The optimum ratio of H₂O₂/O₃ dose was 1.0∼2.0 for both geosmin and 2-MIB. The removal rate constant(k) becomes lower when OH radical consuming materials are present in raw water. The half-life of geosmin decreased rapidly as the O₃ and H₂O₂ doses increase in the sand filtered water. The half life decreased about 8.5 times with the use of 2 mg/L of O₃ and 10 mg/L of H₂O₂ than with the use of 2 mg/L of O₃ alone for the sand filtered water.