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Libo Yu,Xixiang Tang,Shiping Wei,Yinkun Qiu,Xiashutong Xu,Guangxin Xu,Qilin Wang,Qian Yang 한국미생물학회 2019 The journal of microbiology Vol.57 No.7
A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated YLB-03T, with peritrichous flagella was isolated from deep-sea sediment of the Yap Trench at a depth of 4435 m. The bacterium was found to be catalase-positive but oxidase-negative. Growth of this bacterium was observed at 15–50°C (optimum 37°C), pH 5–10.5 (optimum 7), 0–5% NaCl (optimum 1%, w/v) and 0.1–50 MPa (optimum 0.1 MPa). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YLB-03T was a member of the genus Lysinibacillus. Strain YLB-03T was closely related to Lysinibacillus sinduriensis BLB-1T and Lysinibacillus chungkukjangi 2RL3-2T (98.4%), Lysinibacillus halotolerans LAM- 612T (98.0%), Lysinibacillus telephonicus KT735049T (97.5%), Lysinibacillus endophyticus C9T (97.5%), Lysinibacillus composti NCCP-36T and Lysinibacillus massiliensis 4400831T (97.3%). The ANI and the GGDC DNA-DNA hybridization estimate values between strain YLB-03T and closely related type strains were 73.7–76.3% and 34.7–38.7%, respectively. The principal fatty acids were anteiso-C15:0 and iso-C15:0. The G+C content of the chromosomal DNA was 39.6 mol%. The respiratory quinone was determined to be MK-7. The diagnostic amino acids in the cell wall peptidoglycan contained Lys-Asp (type A4α) and the cell-wall sugars were glucose and xylose. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The combined genotypic and phenotypic data showed that strain YLB-03T represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus yapensis sp. nov. is proposed, with the type strain YLB-03T (= MCCC 1A12698T = JCM 32871T).
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Qing Jiao,Libo Zou,Peng Liu,Qian Xu,Yifei Zhang,Ying Yu,Lu Zou,Tianyan Chi,Xuefei Ji 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.10
Xanthoceraside, a saponin extracted from the husks of Xanthoceras sorbifolia Bunge, suppresses inflammation and oxidative stress. However, the antitumor properties of xanthoceraside as well as its mechanism of action remain unclear. Therefore, we proposed to investigate its potential anticancer property. In this study, the viability of cells was measured by the MTT assay. Cell cycle and mitochondrial membrane potential were measured by flow cytometry, and the expressions of procaspase-9, procaspase-3, Cyto.c, Apaf-1, Bcl-2, Bcl-xL, Bad, p53, and IGF-1R/Raf/MEK/ERK were tested by Western blotting. Xanthoceraside significantly inhibited the proliferation of human melanoma A375.S2 cells in a concentration- and time-dependent manner but did not impair the viability of normal cells (peripheral blood mononuclear cells). Further analysis revealed that xanthoceraside induced apoptosis by activating caspase-3 and caspase-9 in a time-dependent manner through the mitochondrial pathway but did not activate caspase-8 in the cells. In addition, xanthoceraside inhibited the expression of the insulin-like growth factor-1 receptor (IGF-1R), which is an important prosurvival, antiapoptotic signaling growth factor receptor that is frequently overexpressed in cancer cells and used as a therapeutic target for multiple cancers. Interestingly, xanthoceraside also decreased the expression of Raf, p-MEK, and p-ERK, the downstream effectors of IGF-1R. Taken together, these findings indicate that xanthoceraside induces apoptosis through a mitochondria-mediated apoptotic pathway, which is induced by the downregulation of IGF-1R/Raf/MEK/ERK cascades in A375.S2 cells.
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Yi Fang,Xiaofang Liu,Libo Zhao,Zhongna Wei,Daoli Jiang,Hua Shao,Yannan Zang,Jia Xu,Qian Wang,Yang Liu,Ye Peng,Xiaoxing Yin 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5
The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, 40 μg/kg i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7- 36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.
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Yaguo Li,Yongbo Wu,Libo Zhou,Masakazu Fujimoto,Jian Wang,Qiao Xu,Shoichi Sasaki,Masaaki Kemmochi 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.
Vibration-assisted grinding, in which harder abrasives than materials to be machined are employed, has been a viable and effective approach to increasing material removal rate (MRR) and/or reducing surface roughness of ground surfaces. We transfer this ideology to fused silica polishing by incorporating ultrasonic vibration into recently developed fixed-abrasive pellets in an attempt to enhance MRR and/or to improve manufactured surface quality. A prototype ultrasonic vibrator, the heart of the polishing head, was designed and the related experimental work was performed on an in-house built setup in conjunction with the constructed head. The vibrator is devised for the generation of 2-D tool path despite using only one actuator in lieu of two actuators in conventional 2-D ultrasonic machining systems. We then combined the ultrasonic vibration with fixed abrasive polishing pellets to machine fused silica glass. Machining experiments reveal that MRR is considerably increased up to >50% upon the introduction of ultrasonic vibration (UV)whilst surface roughness is not degraded appreciably. It was also noted that a overwhelmingly greater deal of polishing debris was dispelled during ultrasonic vibration assisted polishing than conventional bound-abrasive polishing, which may account for the greater MRR in UV assisted polishing.
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Yaguo Li,Yongbo Wu,Libo Zhou,Masakazu Fujimoto,Jian Wang,Qiao Xu,Shoichi Sasaki,Masaaki Kemmochi 한국정밀공학회 2014 International Journal of Precision Engineering and Vol.15 No.5
Vibration-assisted grinding, in which harder abrasives than materials to be machined are employed, has been a viable and effective approach to increasing material removal rate (MRR) and/or reducing surface roughness of ground surfaces. We transfer this ideology to fused silica polishing by incorporating ultrasonic vibration into recently developed fixed-abrasive pellets in an attempt to enhance MRR and/or to improve manufactured surface quality. A prototype ultrasonic vibrator, the heart of the polishing head, was designed and the related experimental work was performed on an in-house built setup in conjunction with the constructed head. The vibrator is devised for the generation of 2-D tool path despite using only one actuator in lieu of two actuators in conventional 2-D ultrasonic machining systems. We then combined the ultrasonic vibration with fixed abrasive polishing pellets to machine fused silica glass. Machining experiments reveal that MRR is considerably increased up to >50% upon the introduction of ultrasonic vibration (UV) whilst surface roughness is not degraded appreciably. It was also noted that a overwhelmingly greater deal of polishing debris was dispelled during ultrasonic vibration assisted polishing than conventional bound-abrasive polishing, which may account for the greater MRR in UV assisted polishing.
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Research and Implement on Intelligent Scheduling of Coal Ash Delivering
Na Zhang,Yuyan Zhang,Wenbo Yu,Libo Xu 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.6
The coal ash sales process in our country is cumbersome, inefficient, lack of effective management and vehicle guidance policy, which leads to frequent traffic jams, ash loss, monitoring difficult and other issues, this paper presents a valid intelligent scheduling strategy for coal ash shipping to design and implement a new coal ash sales system, which proposes a good solution to the problem of coal ash vehicle indiscriminate disorderly, chaotic management
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Expression of Human FGF18 by Fusion with Oleosin in Arabidopsis thaliana Seeds
Chao Jiang,Xiaokun Li,Feng Zhai,Nuo Xu,Jing Yang,Yunpeng Wang,Libo Jin,Haiyan Li 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Expression of recombinant human fibroblast growthfactor 18 (hFGF18) in mammalian cells and Escherichia colihas been extensively used for fundamental research andclinical applications, but they are difficult, expensive. Theexpression of recombinant proteins fused to oleosin proteinhave distinct advantages, such as safety, ease, low cost. Sowe have expressed hFGF18 fused to oleosin protein in the oilbodies of Arabidopsis thaliana (A. thaliana) and screen theproliferation effect of NIH3T3 cells. The vector of oleosinhFGF18fusion gene was constructed and transformed intowild A. thaliana. Transformed A. thaliana lines were obtainedby the floral dip method and confirmed using polymerasechain reaction (PCR). The PCR results indicated that theoleosin-hFGF18 fusion gene was integrated into the A. thaliana genome. The oil bodies expression of oleosin-hFGF18was confirmed by sodium dodecyl sulfate polyacrylamidegel electrophoresis and western blotting. The biologicalactivity showed that oil bodies expressing oleosin-hFGF18could stimulate the proliferation of NIH3T3 cells.
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Fang, Yi,Liu, Xiaofang,Zhao, Libo,Wei, Zhongna,Jiang, Daoli,Shao, Hua,Zang, Yannan,Xu, Jia,Wang, Qian,Liu, Yang,Peng, Ye,Yin, Xiaoxing The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5
The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, $40{\mu}g/kg$ i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7-36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.
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