반정량 식품섭취빈도조사지를 이용한 한국노인의 영양섭취 실태조사

이해정,박선주,김정희,김초일,장경자,임경숙,김경원,최혜미 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.3

The aim of this study was to assess the nutrient intakes of the elderly subjects in Korea. Dietary assessment was carried out using a semi-quantitative food frequency questionnaire (SQFFQ) developed by our laboratory, which included 98 commonly consumed food items selected from 1998 National Health and Nutritional Survey for Korean population. Subjects (n = 2,660) aged 50yr and over were recruited in Seoul, 6 metropolitan cities, and 8 mid-size cities. Calcium and riboflavin intakes of the elderly subjects aged 65 yr and over (n = 1,974) were much lower compared with Korean RDA. Nutrient intakes of the three age group (50 - 64 yr, 65 - 74 yr, 75 yr and over) were decreased as age increased in male and female elderly. Nutrient intakes of male elderly, 75 yr and over, were significantly decreased while in female elderly nutrient intakes were gradually decreased as age increased. Over 30% of the elderly subjects did not meet 75% RDA for calcium, iron, vitamin A, and riboflavin. The proportion of the elderly subjects whose intakes were below 75% RDA was much higher than the elderly whose intakes were above 125% RDA, especially among the elderly aged 75 yr and over. This study revealed that the Korean elderly had inadequate intakes for many nutrients. This will cause a serious nutritional problem for the elderly.

핵전달 방법에 의한 Aspergillus 속 종간잡종 형성에 관하여

김선우,이숙영,양영기 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

핵전달 방법에 의해 A. nidulans FGSC 514와 A. niger의 종간잡종을 얻었고 그들 특성을 관찰한 결과는 다음과 같다. 1. A. nidulans FGSC 514의 원형질체 형성은 삼투안정제인 0.6M KCI과 0.5% Novozym 234를 2시간 처리하여 2x10^7 원형질체를 얻었다. 2. A. niger의 원형질체 형성은 삼투안정제로 0.6M KCI, 0.5% Novozym 234, 0.3% β-glucuronidase와 0.4% BSA를 2시간 처리하여 6x10^7 원형질체를 얻었다. 3. A. niger의핵을 추출하여 A. nidulans FGSC 514 원형질체를 형질전환시켜 3.5%의 형질전환률을 얻었다. 4. 형질전환체의 섬유소 분해효소 활성은 CMCase, avicelase와 pectinase에서 대체적으로 증가하였고 β-glucosidase에서는 모균주보다 약간 감소하는 경향을 보였다. 5. 형질전환체의 건조균체량은 모균주에 비해 대체적으로 높게 나타났고 배양 10시간부터 25시간까지 급격한 증가를 보였으나 40시간 후에는 거의 증가하지 않았다. 6. 형질전환체의 DNA 함량은 1.5∼2.23㎍/10^7pts로 모균주에 비해 약 1.4∼2.0배 증가하였다. 7. 세포외 수용성 단백질은 전기영동 양상은 형질전환체들은 유사하나 모균주의 A. nidulans FGSC 514와 A. niger과는 양상이 다른 중간형태를 나타내었다. The properties of interspecific hybrids obtained by nuclear transfer method between A. nidulans FGSC 514 and A. niger wild type were analyzed by enzyme activity, protein determination, dry weight, DNA content, and total solube protein. The protoplast formation from A. niger was 6x10^7 pts with the treatment of 0.6M KCI, osmotic stabilizer, 0.5% novozym 234, 0.3% β-glucuronidase and 0.4% BSA for 2 hrs. When the isolated nuclei of A. niger wild type were transformed the protoplasts of A. nidulans FGSC 514, transformation frequency was 3.5%. It was found that the enzyme activities in degradation of cellulose and polysaccharide were increased in CMCase, avicelas and pectinase but decreased in β-glucosidase. The strains in which enzyme acttivity in general had a tendency of increasing apecific activity and protein content. The dry weight of transformants were increased more than the parent and increased fast from 10 to 25 hrs. The DNA content of transformants were 1.5∼2.23㎍ and 1.42∼2.04 times more than that of both A. nidulans FGSC 514 and A. niger wild type. Electrophoretic patterns of extracellular solube protein were different between parental and transformant strains.

병원 근로자들의 고용형태에 따른 직무 스트레스와 삶의 질의 관계

전은숙,이강숙,이선영,유재희,홍아름 大韓産業醫學會 2009 대한직업환경의학회지 Vol.21 No.1

목적 본 연구는 병원에 종사하는 정규직 비정규직 근로자의 직무스트레스와 삶의 질 수준을 파악하고 직무스트레스와 삶의 질과의 관련성 및 삶의 질에 영향을 미치는 요인을 규명하기위해 수행되었다 방법 자기기입형 설문조사를 실시하였으며 정규직 172명 비정규직 189명이 최종 분석하였다 일반적 특성과 한국인 직무스트레스 측정도구-단축형 (Korean occupational stress scale short form) 삶의 질 측정을 위한 도구 WHOQOL-BREF를 사용하였다 결과 정규직에 비해 비정규직에서 직무스트레스 수준이 높았다 정규직은 직무요구도와 직장문화의 항목에서 비정규직은 직무자율성 직무불안정성 보상부적절의 항목에서 직무스트레스 요인이 유의하게 많았다 삶의 질수준은 정규직 근로자에서 유의하게 높았으며 심리적 영역 사회적 영역 생활환경 영역에서 비정규직보다 유의하게 높은 수준을 보였다 결론 정규직과 비정규직 근로자의 직무스트레스 요인과 삶의 질 수준은 달랐다 고용형태를 막론하고 직무스트레스는 삶의 질에 부정적인 영향을 미치고 있었으며 비정규직은 직장문화로 인한 스트레스 요인이 많을수록 삶의 질이 낮았다 따라서 병원근로자의 삶의 질 향상을 위하여 적절한 관리 혹은 프로그램이 제공되어야 할 것이며 특히 비정규직 근로자의 직무스트레스를 일으키는 요인들에 대한 적절한 관리가 필요할 것이다 Objective This study was conducted to evaluate the relationship between job stress and quality of life for hospital workers by type of employment Methods Data were obtained for 361 workers in a large hospital 172 of whom were categorized as typical workers defined by permanent employee and 189 of whom were categorized as atypical workers defined by fixed term contraction Job stress was assessed using the Korean Occupational Stress Scale Short Form and the World Health Organization Quality of Life-BREF Questionnaire Results Atypical workers had significantly higher scores for job related stress in the domains Of insufficient control over work job insecurity and lack of reward in the workplace compared with typical workers who had higher scores for stress in the domains of job demands and occupational climate Test scores also indicated that typical workers had a significantly better quality of life than atypical workers especially in terms of mental health social relationships and environment Conclusion These findings suggested that factors contributing to job-related stress were different between typical and atypical hospital and typical workers are likely to have a better quality of life

그레이큐브에서 정렬 및 전위합 알고리즘

주낙근,박기선,이숙희 東新大學校 1998 論文集 Vol.10 No.-

In this paper, we present algorithms, which solve the problems of sort and prefix-sums on graycube. The complexities of these algorithms are O(log^2n) and O(log n) respectively. The graycube is a new interconnection network for parallel computer. it has lower diameter and average distance than that of hypercube and it's variants. Also it has many attractive properties.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

보건소를 이용하는 지역주민의 건강 행위와 건강관련 체력간의 관계

전미양,최명애,이인숙,김태수,김의숙,박선홍 대한보건협회 2002 대한보건연구 Vol.28 No.3

The purpose of this study is to identify relationship health behavior and health related physical fitness in order to provide the basic data for health promotion program for community people. The study was conducted 299 people, during the period from June 1, 2000 to October 30, 2000. The result are as follows. 1. Female comprised was 63.2%(189), while male comprised 36.8%(110). The age range of the subject were from 21 to 59 years old. The subject were aged 30s group 52.5%, 40s group 29.1%, 50s group 12.7%, 20s group 5.7%. Twenty subjects (6.7%) had experience smoking and one subject (0.3%) in female and nineteen subjects (6.4%) in male. Seventy-five subjects (25.1%) had experience drinking and twenty-nine subjects (9.7%) in female, and forty-six (15.4%) in male subjects. ninety-nine subjects had exercise and sixty subjects (20.1%) in female subjects and thirty-mine subject (13%) in male. 2. Body fat and flexibility were higher in female than male subjects, but cardioresperatory endurance, muscular strength and muscular endurance were higher in male than female subjects. 3. There were significant differences in body fat and muscular endurance between age group in both gender subjects, and in cardioresperatory endurance and muscular strength between the age group in male subjects. 4. No significant differences health related fitness between non-smoking and smoking group in both gender subjects. 5. No significant differences health related fitness between non-alcohol and alcohol group in both gender subjects. 6. For female subjects, muscular strength, muscular endurance and flexibility of the exercise group were significantly higher than those of the non exercise group. for male subjects, cardiorespiratory endurance of the exercise group were significantly higher than those of the non exercise group.

침지조작에 의한 레스베라트롤 증가조건의 최적화

이선숙(Seon-Sook Lee),서선정(Sun-Jung Seo),이부용(Boo-Yong Lee),이희봉(Hee-Bong Lee),이준수(Junsoo Lee) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.4

레스베라트롤은 항암작용, 항혈전작용, 항산화작용 및 심혈관계질환의 발병을 낮추는 작용이 있다고 보고되어 있다. 본 실험에서는 땅콩에 함유되어 있는 레스베라트롤의 함량을 증가시키기 위한 침지조건을 반응표면분석법을 이용하여 최적화하였다. 레스베라트롤의 함량에 영향을 미치는 요인으로는 침지 시 물의 양(농도), 침지시간 그리고 침지 후 건조시간을 검토하였으며, 실험결과 침지 시 물의 양(농도)은 67.15%, 침지시간은 19.57 hr, 침지 후 건조시간은 65.56 hr으로 나타났다. 이 조건하에서 레스베라트롤은 0.1 ㎍/g에서 4.55 ㎍/g으로 약 45배 정도 증가함을 확인할 수 있었다. 레스베라트롤의 증가에 가장 큰 영향을 주는 요인으로는 침지시간이었으며 그 다음으로 침지 시 물의 양(농도)이 영향을 주었으며, 침지 후 건조시간은 별 영향을 주지 않았다. 본 실험의 이차 다항 회귀 곡선식에 대한 적합도를 결정계수로 조사한 결과 높은 적합도를 나타내었으며, 분석 방법에 대한 검증에서도 그 결과가 우수하였다. In this study, the effects of varying the amount of water, soaking time at 25℃ and drying time after soaking at 45℃ on the induction of resveratrol were evaluated to optimize the soaking treatment by response surface methodology (RSM). After response surface regression (RSREG), the second-order polynomial equation was fitted to the experimental data. The analysis of variance showed that the model appeared to be adequate (R² =0.9547) with no significant lack of fit (p>0.1). From statistical analysis, amount of water and soaking time were found to be significant factors. On the other hand, drying time was not significant. Ridge analysis showed that the optimized parameters were 67.15% for amount of water, 19.58 hr for soaking time, 65.56 hr for drying time. It was confirmed that resveratrol content was increased from 0.1 ㎍/g to 4.55 ㎍/g under the optimized conditions. In addition, the experimental values at the optimized condition agreed with values predicted by ridge analysis. The analytical method validation parameters such as accuracy, precision, and specificity were calculated to ensure the method's validity.

Amelioration of sepsis by TIE2 activation–induced vascular protection

Han, Sangyeul,Lee, Seung-Jun,Kim, Kyung Eun,Lee, Hyo Seon,Oh, Nuri,Park, Inwon,Ko, Eun,Oh, Seung Ja,Lee, Yoon-Sook,Kim, David,Lee, Seungjoo,Lee, Dae Hyun,Lee, Kwang-Hoon,Chae, Su Young,Lee, Jung-Hoon American Association for the Advancement of Scienc 2016 Science translational medicine Vol.8 No.335

<P>Protection of endothelial integrity has been recognized as a frontline approach to alleviating sepsis progression, yet no effective agent for preserving endothelial integrity is available. Using an unusual anti-angiopoietin 2 (ANG2) antibody, ABTAA (ANG2-binding and TIE2-activating antibody), we show that activation of the endothelial receptor TIE2 protects the vasculature from septic damage and provides survival benefit in three sepsis mouse models. Upon binding to ANG2, ABTAA triggers clustering of ANG2, assembling an ABTAA/ANG2 complex that can subsequently bind and activate TIE2. Compared with a conventional ANG2-blocking antibody, ABTAA was highly effective in augmenting survival from sepsis by strengthening the endothelial glycocalyx, reducing cytokine storms, vascular leakage, and rarefaction, and mitigating organ damage. Together, our data advance the role of TIE2 activation in ameliorating sepsis progression and open a potential therapeutic avenue for sepsis to address the lack of sepsisspecific treatment.</P>

임신 중기 태아 맥락총 낭종 119예의 추적 조사: - 18번 삼염색체증과의 연관성 여부를 중심으로 -

이은혜(Eun Hye Lee),이유미(You Me Lee),신명철(Myung Choel Shin),민유선(Yu Seon Min),이상희(Sang Hee Lee),김현철(Hyeon Chul Kim),김종욱(Jong Wook Kim),이숙환(Sook Hwan Lee),이위현(Wee Hyun Lee),조진호(Jin Ho Cho),이정노(Chung No Lee) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7

Objective: To evaluate the clinical significance of fetal choroid plexus cysts (CPCs) in the second trimester, especially an association with trisomy 18. Methods: From March 1998 through June 1999, second trimester screening ultrasonography was performed on 4,948 unselected single-ton pregnancies. CPCs were noted in 132 fetuses. Among them, detailed ultrasonography and follow-up was possible in 119 cases and they were recruited into the study. There were 91 cases of isolated CPCs and 28 cases of CPCs in high-risk population. Isolated CPCs were defined as: mother did not have any risk factors requiring amniocentesis and there were no other sonographic abnormalities on detailed ultrasound. CPCs in high-risk population were defined as: mother had any risk factor requiring karyotyping or there were any other sonographic abnormalities although she was general population. Amniocentesis was performed in 39 cases. We compared gestational age at time of detection, size, bilaterally, multiplicity, and complexity of CPCs in the group of isolated CPCs and CPCs in high-risk population (t-test, chi-square test; p<0.05). We evaluated the findings of detailed and follow-up ultrasonography, karyotypes, and final outcomes of pregnancy.Results: Gestational age at time of detection was not different in both groups of isolated CPCs and CPCs in high-risk population (19±2 vs 18±1 wk, p>0.05). Mean size (6.4 vs 6.2 mm), bilaterality (60% vs 57%), multiplicity (66% vs 57%), and complexity (8% vs 14%) of CPCs were also similar. All CPCs were disappeared irrespective of size and mean time of disappearance was 25±3 and 26±3 week, respectively (p>0.05). All cases of isolated CPCs resulted in phenotypically-normal neonates. It was confirmed by either amniocentesis or postnatal examination by the pediatrician. Among fetuses having CPCs in high-risk population, two trisomy 18 and one trisomy 21 were detected. All of them had positive result of maternal serum marker test and/or sonographic abnormalities. Remaining cases were proved normal.Conclusion: The risk of chromosome abnormalities is very high when CPCs are associated with other abnormalities on detailed ultrasound, indicating a clear need to offering genetic amniocentesis. As contrast, the risk of chromosome abnormalities for a case of isolated CPCs is very low, and in this series there was no trisomy 18. Therefore isolated CPCs should be considered as the indication of detailed ultrasound examination, but not routine karyotyping.

일개 전자산업 남자 근로자들의 재흡연 관련 요인

이강숙,이정운,이원철,맹광호,양선임,김대석,이선영,이경화 가톨릭대학산업의학센타 산업의학연구소 2003 韓國의 産業醫學 Vol.42 No.2

This study was carried out to find the factors associated with relapse of smoking in mail workers. The data were obtained from 513 workers of an electronics industries in October 2002 about nicotine dependence, withdrawal symptom, and knowledge for smoking. The results of this study can be summarized as follows; 1. Amount and duration of smoking, irregular eating and non-exercise were higher in re-smokers. 2. The rate of high nicotine dependency and craving for smoking was increased in re-smoker. 3. Mean of quitting trial was 2.39 in ex-smokers and 2.17 in re-smokers, the duration of first quitting were 10.43 days in ex-smokers and 3.73 days in re-smokers. 4. The top reason for re-smoking was 'job-stress' in ex-smokers and re-smokers. 5. The mean score of knowledge about smoking was 7.88 in ex-smokers and 7.83 in re-smokers. 6. The more nicotine dependency increased, the more withdrawal symptom becomes. And increased withdrawal symptoms are correlated with frequency of quitting trial positively. In conclusion, it was suggested that factors of re-smoking were job-stress and nicotine dependency. Therefore comprehensive smoking cessation program including stress management and nicotine replace therapy was needed.