http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lignification in Relation to the Influence of Water-deficit Stress in Brassica napus
Lee, Bok-Rye,Zhang, Qian,Kim, Tae-Hwan The Korean Society of Grassland and Forage Science 2014 한국초지조사료학회지 Vol.34 No.1
To investigate lignification process and its physiological significance under water-deficit condition, the responses of peroxidases, polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in relation to leaf water status to the short term of water deficit treatment in the leaves with different maturities in forage rape were measured. The significant decrease in relative water content (RWC) and leaf osmotic potential (${\Psi}{\pi}$) were apparent after 5 d of water-deficit treatment. The activity of guaiacol peroxidase (GPOD), ascorbate peroxidase (APOD), coniferyl alcohol peroxidase (CPOD), and syringaldazine peroxidase (SPOD) was depressed especially in middle and old leaves when compared with that of control leaves. On the other hand, in young leaves, a significant increase in CPOD (+34%) and SPOD (+24%) activity as affected by water-deficit treatment was apparent. The activation of PAL and PPO was observed in middle and old leaves for PAL and in young and middle leaves for PPO. These results suggest that peroxidases in middle and old leaves did not involve in lignification under mild water-deficit stress, whereas CPOD and SPOD in young leaves participate in lignification by a coordination with PAL and PPO to incorporate phenol and lignin into the cell walls.
Bok-Rye Lee,Yun-Ju Baek,Seung Gon Wi,Ung Yang,Sang-Hyun Lee 한국원예학회 2021 원예과학기술지 Vol.39 No.1
This study aimed to investigate the relationships among fruit size and protein metabolism and profiles during fruit development and ripening of three Asian pear (Pyrus pyrifolia Nakai) cultivars predominantly grown in Korea. The final fruit dry weight was higher in the late-maturing cultivar ‘Niitaka’ than in the early-maturing cultivars ‘Wonhwang’ and ‘Whangkeumbae’. After 87 days after full bloom (DAFB), the fruit amino acid content significantly decreased in ‘Niitaka’ and ‘Wonhwang’ pears, while they remained stable at lower levels in the ‘Whangkeumbae’ pears during fruit development and ripening. At the beginning of our sampling period (57 DAFB) the protein content was the highest in ‘Niitaka’, followed by ‘Wonhwang’ and ‘Whangkeumbae’. The fruit protein content gradually decreased to 117 DAFB for ‘Niitaka’ or 102 DAFB for ‘Wonhang’ and ‘Whangkeumbae’, and then maintained at the similar level to the last sampling date. These reductions in the protein content were consistent with the increases in the protease activity. A rapid increase in the protease activity was observed in the ‘Niitaka’ and ‘Wonhwang’ pears during the early stage of fruit development, whereas a gradual increase was observed in the ‘Whangkeumbae’ pears during the entire period analyzed. The protein pattern varied across the various fruit developmental stages, revealing up- and down-regulated proteins during fruit development as well as highly expressed proteins at the middle phase of the period evaluated. Our results indicate that protein degradation by proteases in the early stage of fruit development, which in turn determines the fruit amino acid content, may affect the final size of Asian pears.
Bok-Rye Lee,Jeong-Hwa Cho,Seung Gon Wi,Ung Yang,Woo-Jin Jung,Sang-Hyun Lee 한국원예학회 2021 원예과학기술지 Vol.39 No.2
This study investigated the regulatory mechanism controlling fruit maturity, focusing on sugar metabolism during pear fruit development and ripening. Three Asian pear cultivars, ‘Wonhwang’ (early-maturity), ‘Whangkeumbae’ (early-maturity), and ‘Niitaka’ (late-maturity), were selected. Absolute growth rate was the highest at 117 days after full bloom (DAFB) in early-maturing cultivars, ‘Wonhwang’ and ‘Whangkeumbae’, and at 147 DAFB in the late-maturing cultivar, ‘Niitaka’. Hexose (glucose and fructose) content was relatively higher in ‘Wonhwang’ and ‘Whangkeumbae’ than in ‘Niitaka’ during the early stage of fruit development, concomitant with an increase in the activities of cell wall invertase (CWINV), vacuolar invertase (VINV), and neutral invertase (NINV). During the late stage of fruit development, sucrose content and the sucrose-to-hexose ratio were relatively higher in ‘Wonhwang’ and ‘Whangkeumbae’ than in ‘Niitaka’, accompanied by an increase of sucrose phosphate synthase (SPS) activity involved in sucrose re-synthesis. Principal component analysis (PCA) distinctly separated the fruit development into two parts, showing a shift in the early to late stage of fruit development, consistent with the point when the sucrose-to-hexose ratio began to increase. During the late stage of fruit development and ripening, the sucrose-to-hexose ratio was positively correlated with fresh weight, CWINV, and SPS activities and negatively correlated with starch or NINV and VINV activities. These results indicate that the sucrose-to-hexose ratio is an important factor in regulating fruit maturity and is modulated by invertases and SPS activities.
Lee, Bok-Rye,Muneer, Sowbiya,Avice, Jean-Christophe,Jung, Woo-Jin,Kim, Tae-Hwan Springer-Verlag 2012 Mycorrhiza Vol.22 No.7
<P>To compare the effect of arbuscular mycorrhiza (AM) and P-supplement on N uptake and N assimilation under well-watered or drought-stressed conditions, Glomus intraradices-colonised, P-supplemented non-mycorrhizal (P) and non-mycorrhizal (control) plants of Lolium perenne were exposed to 12 days of water treatment. Leaf water potential (ψ (w)), photosynthetic ability, and N and P nutritional status were measured at the beginning (day 0) and end (day 12) of water treatment. N absorption, amino acid and protein synthesis were quantified using the isotopic tracer (15)N at day 12. Under well-watered conditions, growth response and physiological parameters were similar in AM and P plants, as compared to controls. Drought (10% water) significantly decreased these parameters in all three treatments. As compared to control plants, the negative impact of water deficit on the ψ (w), photosynthesis, biomass, and N and P content was highly alleviated in AM plants, while only slightly improved or remained the same level in P plants. The effect of AM symbiosis on N absorption and N assimilation was greater than that of the P supplement under well-watered and drought-stressed conditions, and this effect was highly enhanced under drought-stressed conditions. At terminal drought stress on day 12, the effect of AM colonisation on de novo synthesis of amino acids and proteins was 4.4- and 4.8-fold higher than that of the P supplement. These results indicate that the AM symbiosis plays an integrative role in N nutrition by alleviating the negative impacts of drought on N or P uptake and N assimilation, whereas the efficiency of a direct P supplement is very limited under drought-stressed conditions.</P>