요통 환자의 운동 치료

고성경,구민석 大邱大學校附設 基礎科學硏究所 2004 基礎科學硏究 Vol.21 No.1

The purpose of this study is to review the exercise therapy for the low back pain patients. The vertebral column consist of 26 vertebrae. Among the vertebrae, the lumbar vertebrae are heaviest and biggest because of concentration of body weight. L3-L5, S1, between lumbar curvature and Sacrum, are frequent body sites for the low back pain. The risk factors of low back pain are the life-style as like occupation and work, and personal characteristics as like age, sex and history. The exercise for the low back pain patients recommend stretching for the trunk flexibility, abdominal training for muscular strength, and whole body exercise for physical fitness. These exercise should be practice during 3-6 months except for patients with contraindications.

Raphanus sativus Var. Chungpihongsim의 hairy root 배양에 의한 anthocyanin 생성

황성진,백윤웅,고경민,안준철,황백 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-

청피홍심무우를 재료로 A. rhizogenes A₄균주를 접종하여 hairy root를 유도하였으며 유도된 hairy root는 paper electrophoresis를 통하여 mannopine과 agropine이 검출되어 형질전환 되었음을 확인하였다. Hairy root는 MS의 기본염을 1/2로 희석시킨 1/2 MS, sucrose 3%, pH 5.2로 조성된 배지에서 최적인 성장을 보였다. 유도된 hairy root는 각 strain사이에 색소능에 다소간의 차이를 보였으며 2, 4-D kinetin의 적절한 조합의 처리구에서는 탈분화와 더불어 색소가 형성되었고, 그 중 2, 4-D와 kinetin을 각각 1 ppm, 5 ppm 처리하였을 때 최대 색소능을 보였다. hairy root의 탈분화시 형성되는 색소는 원 식물 뿌리의 주 색소인 pelargonidin 계열의 색소로 추정되며 총 색소량은 49mg/100g(F.W.)로 잠정 계산되었다. Hairy root induced following inoculation of the root disc and plantlet fragment of R. sativus Var Chungpihongsim with A. rhizogenes A₄. The hairy root contained agropine and mannopine. Medium contained 1/2 MS salt, sucrose 3%, pH 5.2 was optimized to growth of hairy root. Depending on the hairy root line, the level of pigment in hairy root varied somewhat . When 2, 4-D and kinetin were added in hairy root, the synthesis of pigment induced with deorganization of hairy root. Especially, addition of 2, 4-D 1 ppm and kinetin 5 ppm showed the maximum synthesis of pigment. Compared with anthocyanin extract of mature root three pigments were assumed to acylated pelargonin. The content of anthocyanin in these sample was 49mg/100(F.W.).

The enhancing method of ginsenoside production for hairy root mass cultures of ginseng ( Panax ginseng C . A meyer )

Kyeong Min Ko,Baik Hwang,Deok Chun Yang,Ji Chang Park,Kwang Tae Choi 한국식물생명공학회(구 한국식물조직배양학회) 1996 한국식물생명공학회 춘계학술연구발표회 Vol.1996 No.-

패션이미지에 의한 남자대학생 의류시장 세분화

고태경,김영주,김주희,명혜민,안민정,이민경,이현정,조희래,강원창 연세대학교 생활과학대학 1992 婦學 Vol.25 No.-

A New Diarylpropane from the Stem Bark of Lindera erythrocarpa Makino

Ko, Ryeo-Kyeong,Kang, Min-Chul,Jin, Yeon-Jun,Choi, Ho-Min,Kim, Bong-Seok,Han, Jong-Heon,Kim, Gi-Ok,Lee, Nam-Ho Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.3

Pathogenic E. coli Inactivation in Upland Soils to a Change of Soil Moisture Content and Temperature

Min-Kyeong Kim(김민경),Min-Young Kim(김민영),Chul-Man Choi(최철만),Byong-Gu Ko(고병구),Soon-Ik Kwon(권순익),Hang-Yeon Weon(원항연) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.1

전 세계적으로 가축분뇨를 농경지에 시용하는 것은 작물과 토양에 양분을 공급하는 측면에서 오래 전부터 이용되어 왔으며, 최근 들어서는 자원을 재순환하기 위한 측면으로 이용되고 있다. 그러나, 환경적인 측면에서 가축분뇨를 농경지에 시용하였을 때 미생물적 평가나 규제기준은 비용과 시간이 많이 요구되어 우리나라에서는 이에 대한 연구가 수행되지 못한 실정이다. 따라서, 본 연구는 밭토양에서 토양수분과 온도조건에 따른 분변성 대장균의 사멸율을 조사하여 분변성 대장균이 외부 환경에 영향을 미칠 수 있는 수준을 평가하고자 수행하였다. 시료는 토양수분조건(10, 35, 65, 80%) 및 온도조건(20, 25, 30℃)별로 정해진 기간 (3, 7, 14, 21, 28일) 동안 배양되었으며 각각의 시료에 포함된 대장균의 수는 plating method 를 이용하여 측정되었다. 분산분석을 통한 측정자료의 통계분석 결과, 분변성 대장균의 생존에는 토양수분함량과 온도가 주 요인이었는데, 특히 토양수분함량이 감소할수록 그리고 온도가 증가할수록 E. coli의 사멸율은 직선적으로 증가하였다. 또한, 토양수분 조건과 온도조건 중에서 분변성 대장균의 사멸율은 온도조건에 더 상관이 높은 것으로 나타났으며, 모든 온도조건에서 10%의 토양수분조건에서 분변성 대장균의 사멸율이 높았다. 이는 토양수분이 E. coli의 사멸에 제한적인 요인으로 작용하였음을 의미한다. 따라서, 본 연구는 가축분뇨를 농경지에 시용할 때 인체의 위해성과 외부 환경의 건전성을 유지하기 위해서는 고온 저습한 토양조건에서 시용하여야 한다는 현실적인 가이드라인을 제시하고자 한다. The application of livestock manure to cropland is a practice that has been used for centuries. Agricultural crops can utilize nutrients from manure, and the producer can utilize land for disposal, although in a “sustainable system” the concept is manure utilization and not waste disposal. However, meeting regulatory criteria regarding microbial quality remains an expensive and time consuming process. The purpose of this study was to quantify the level of environmental impact of soil moisture and temperature on fecal coliform (Escherichia coli or E. coli) survival in upland soils for sound application of livestock manure. Samples were collected up to 30 days depending on the given conditions. The inactivation rate of E. coli increased linearly with increased temperature while the inactivation rate gradually decreased with decreased soil moisture level. The overall findings of this study showed that the temperature was the limited factor on E. coli survival in soils over soil moisture content. This study will provide useful and practical guidelines to applicators of soil in deciding appropriate handling and time frames for land application for sustainable agriculture.

2단계 배양방법을 이용한 인삼 모상근의 (毛狀根) 대량배양과 Ginsenoside 생산

고경민(Kyeong Min Ko),양덕춘(Deok Chun Yang),박지욱(Ji Chang Park),최강주(Kang Ju Choi),최광태(Kwang Tae Choi),황백(Baik Hwang) 한국식물학회 1996 Journal of Plant Biology Vol.39 No.1

A hairy root clone of Panax ginseng C.A. Meyer, HRB-15 was cultured in various conditions with 3 L bubble type bioreactor to enhance both growth and ginsenoside production. The hairy roots were more rapidly grown under the dark condition than under the light condition. However, total amount of ginsenoside of hairy roots cultured under the light for 30 days increased 2 folds as compared with the dark condition and was 1.10% based on 6 ginsenosides. Especially, ginsenoside-Re was significantly increased and some ginsenosides except for ginsenoside-Re was slightly reduced. Also, the growth of hairy roots decreased about 30% as compared with the dark condition. In contrast, addition of sodium acetate led to decreased production of ginsenoside and growth of hairy roots under ligt condition. The influence of potassium dihydrogenphosphate concentration was examined in MS medium and a 1.25 mM concentration was found to be the most appropriate for growth and ginsenoside production under light condition. Two-step process of hairy roots culture with yeast elicitation or without ammonia in culture medium was developed to enhance growth and ginsenoside synthesis. 50 ㎍ of yeast elicitor per g of fresh weight showed a synergistic effect on the ginsenoside synthesis of hairy roots on 20 days after culture. At that time, the content of total ginsenoside was 1.15%, while the growth of hairy roots decreased 21% as compared with the dark condition. In addition, when elimination of ammonia on 20 days after culture, the content of total ginsenoside was 1.26% with significant increment of ginsenoside-Rd (0.27%) in addition to ginsenoside-Re and the growth of hairy roots decreased 10% as compared with the dark condition. In this system, we have demonstrated a unique two-step process of hairy root cultures to maximize biomass and secondary metabolites. It has found possibility to enhance ginsenosides production by growing hairy roots in this method.

인삼 모상근 (毛狀根) 배양에 의한 Anthocyanin 의 생산과 동정

고경민(Kyeong Min Ko),최양순(Yang Soon Choi),황백(Baik Hwang) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.1

In hairy root cultures of ginsegn (Panax ginseng C.A. Meyer) transformed by Agrobacterium rhizogenes, the effects of light, carbon source and various hormone on hairy root growth and anthocyanin production were investigated. Anthocyanine synthesis began to first occur 5 days after exposure to light, and then maximum yield of anthocyanin was 0.36 mg/g(frwt) in MS medium after 30 days. Of the nutritional factors concentration of 60 mM mitrogen and sucrose as a carbon source showed market effects on the growth and anthocyanin production MS medium supplemented with 0.5 mg/L IAA was most suitable for the hairy root proliferation, and the best accumulation of anthocyanin was obtained at 1 mg/L IAA treatment (0.41 mg/g, frwt). Whereas 2,4-D tended to restrain the pigment synthesis. From the isolation and identification of anthocyanin pigments, main anthocyanin in ginseng hairy root was identified as pelargonidin-glucoside.

Bi-LSTM 기반 CycleGAN을 활용한 음악 해석 생성 모델

고경민(Kyeong-min Ko),박종화(Jong-hwa Park) 한국정보통신학회 2023 한국정보통신학회 종합학술대회 논문집 Vol.27 No.1

다음 전자음악 시장은 점점 커지고 있으나 컴퓨터를 이용한 연주와 사람의 연주 사이 간격은 좁혀지지 않고 있다. 따라서 본 연구에서 AI를 통해 음악 해석을 생성함으로써 이 간격을 줄이고자 했다. 이를 위해 CycleGAN을 구조체로 사용하고 그 배누구조는 음악 데이터를 처리할 수 있도록 Bi-LSTM과 TimeDistributed layer를 활용해 모델을 설계하고 학습시켰다. 하지만 CycleGAN의 불안정한 학습 과정으로 인해 결과물에서 과적합이 발생했다. 향후 연구에서는 내부 구조와 손실함수 등을 변경하여 모드 붕괴를 해결할 수 있을 것이다. 또한 연주자의 정보에 해당하는 태어난 시대나 음악 양식을 직접적으로 활용한다면 보다 발전된 결과물을 얻을 수 있으리라 기대한다. The electronic music market is growing, but the gap between computer performance and human performance has not narrowed. Therefore, in this study, we tried to reduce this gap by generating music interpretation through AI. To this end, CycleGAN was used as a structure, and its internal structure was designed and learned using Bi-LSTM and TimeDistributed layer to process music data. However, due to the unstable learning process of CycleGAN, overfitting occurred in the results. In future studies, mode collapse can be solved by changing the internal structure and loss function. In addition, it is expected that more advanced results will be obtained by directly utilizing the era of birth or music style corresponding to the performers information.

Agrobacterium rhizogenes 에 의하여 형질전환된 인삼 모상근의 세포 유전학적 및 조직학적 특성

고경민(Kyeong Min Ko),송재진(Jae Jin Song),황백(Baik Hwang),강영희(Young Hee Kang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1

Ginseng (Panax ginseng C.A. Meyer) hairy root transformed with Agrobacterium rhizogenes (strain A_4) was examined cytogenetically and histologically to assess its characteristics. The optimum growth of hairy root obtained in hormone-free MS medium (sucrose 30 g/L, pH 5-6) for long period cultures. All hairy root strains (HB1, HB2, HB3) had the 2n diploid number (2n = 48) of chromosomes in root tip cells. There were no alteration in chromosome structure except in one cell of HB3 strain. Results of SDS-PAGE showed a few difference in pattern and number of bands between normal and hairy root of ginseng. The root anatomy of normal root and hairy root differed each other. The hairy root had a clearly defined vascular strand, and the morphology of cortical cells were disorganised with large intercellular spaces.