Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

Biosimilar Development And Case Study in Celltrion

Ki?Sung Kwon 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4

1930년대 야담잡지 소재 연성흠의 야담 기술과 의미

권기성(Kwon, Ki-Sung) 우리문학회 2023 우리文學硏究 Vol.- No.79

1920년대부터 야담은 동화와 긴밀히 접촉하고 있었다. 다만 그 방법은 동화가 야담을 활용하는 정도였으며, 연구자의 관심도 여기에서 벗어나지 않았다. 그런데 1930년대부터 야담 잡지의 기획자는 동화작가들을 소환하여 그들이 지닌 소재의 유사성 등을 활용하여 야담을 작성하도록 한다. 그 차이를 살피기 위해 본고에서는 연성흠이 『월간야담』과 『야담』 잡지에 남긴 작품에 주목했다. 연성흠의 야담은 대개 전시기 야담집에 수록된 작품을 준용하는 특징을 보이지만, 필요한 경우가 있다면 적극적 변개도 주저하지 않았다. 그리고 이런 편찬과 서술의 특징은, 한결같이 선한 인물의 시혜와 보은이라는 지점을 강조하면서 ‘공동체 윤리’를 강조한 주제적 지향을 견인했다. 이는 그의 동화작법과 퍽 유사한 것이었다. 한편 야사를 토대로 새로운 이야기를 만들어낼 때는 통속과 비극이라는 당대의 ‘애사’ 글쓰기를 답습하는 경향도 보였다. 곧 야담과 야사의 글쓰기 방식이 미세하게 달랐던 셈이다. 논문에 제시된 연성흠의 작품을 통해 두 잡지의 기획이 다양한 형태로 이루어졌으며, 독자를 유인하는 기획의 소산이었음을 짐작해 볼 수 있었다. 이는 곧 야담 집필진 저변의 다양화를 통해 활로를 모색한 가운데, 아동문학과의 접속이 주요한 역할을 했던 것이다. 그 저변에는 지면의 확대에 따른 다양한 글쓰기의 가능, 개인의 경제적 사정, 친분 등 여러 이유가 있었다. 결과적으로 30년대 동화작가들이 야담 잡지에 참여하고 다양한 야담 기법을 시도하면서, 당시 야담의 흥행을 견인하는 일익을 담당하고 있었던 것이라 할 수 있다. Since the 1920s, Yadam has been in close contact with fairy tales. However, the method was to the extent that fairy tales used night stories, and the researcher’s interest did not deviate from this. However, from the 1930s, the organizers of Yadam magazine summoned fairy tale writers to write Yadam by utilizing the similarity of their materials. In order to examine the difference, this paper focused on the works left by Yeon Sung-heum in 『Wolgan-Yadam』 and 『Yadam』 magazines. Yeon Sung-heum’s Yadams usually show the characteristics of applying mutatis mutandis to the works contained in the exhibition’s Yadams, but he did not hesitate to actively transform himself if necessary. And these characteristics of compilation and description drove the thematic orientation that emphasized “community ethics” while emphasizing the point of the benefit and complement of good characters. This was very similar to his fairy tale writing. On the other hand, when creating a new story based on wild events, there was also a tendency to follow the writing of the “Sad-history” of the time of popularity and tragedy. In other words, the writing methods of Yadam and Yasa were slightly different. Through the work of Yeon Seong-heum presented in the paper, it was possible to guess that the planning of the two magazines was carried out in various forms, and that it was the product of the planning that attracted readers. In other words, the connection with children’s literature played a major role while seeking a way out through the diversification of the base of the Yadam writers. There were various reasons at the base, such as the possibility of various writing due to the expansion of the space, individual economic circumstances, and friendship. As a result, it can be said that in the 30s, fairy tale writers participated in Yadam magazines and tried various Yadam techniques, playing a role in driving the success of Yadam at that time.

양 족부 열감을 호소하는 하지불안증후군 환자 치험 1례

김경묵,서원주,김수빈,정우상,문상관,권승원,조기호,Kim, Gyeong-muk,Suh, Won-joo,Kim, Su-bin,Jung, Woo-sang,Mun, Sang-kwan,Kwon, Seung-won,Cho, Ki-ho 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.2

Objectives: The purpose of this study is to evaluate the effectiveness of electroacupuncture on heat sensation of the feet of a 61-year-old female patient with restless leg syndrome. Methods: Electroacupuncture was performed at four acupoints (KI3 (太谿), KI6 (照海), LR3 (太衝), LR2 (行間)) selected to reduce heat sensation in both feet. The patient's symptoms were assessed using the VAS (Visual Analogue Scale), the SF-MPQ (short-form McGill Pain Questionnaire), and the IRLS (International Restless Legs Scale). The treatment was performed from April 20, 2010 to April 24, 2010. Results: During five sessions of electroacupuncture treatments, the VAS, SF-MPQ, and IRLS scores improved throughout the treatment period and afterwards. Conclusions: The results suggest that electroacupuncture at KI3(太谿), KI6(照海), LR3(太衝), LR2(行間) can be effective for reducing hot flush in both feet.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

Cinnamonitrile의 酸 加水分解 反應(Ⅰ)

成洛道,權寄星 圓光大學校 基礎自然科學硏究所 1982 基礎科學硏究誌 Vol.1 No.1

The rate constants for the hydrolysis of cinnamonitrile at pH 0.0~7.0 range are determined by UV spectrophotometry in 50% methanol at 25℃. Analysis of rate equation and the general base catalysis, in the hydrolysis of cinnamonitrile therefore it appears probable that the attack by a nucleophile, water molecule occurs at the increased positive charge α-carbone atom and the αC-βC bond scission follow subsequently in acidic and neutral media. Conformation of (E)-cinnamonitrile have been studied by MO theoretically using extended Hu¨ckel theory(EHT) and CNDO/2 molecular orbital calculation methods. The results show that the most preferable conformation of the phenyl ring rotamer is the coplaner forms with regard to the C_7(α)=C_8(β)plane.

Ethylcrotonate에 對한 Iso-propylmercaptan의 親核性 添加反應

權奇星,朴文奎,成洛道 충남대학교 자연과학연구소 1974 學術硏究誌 Vol.1 No.1

The rate constants for the addition reaction of iso-propylmercaptan to ethylcrotonate have been measured by iodometry and for the proposed reaction mechanism a rate equation which can be applied over wide pH range was derived. From this rate equation, it was found that below pH 6.0 the reaction is started by addition of iso-propylmercaptan molecule and above pH 11.0 this reaction is proceeded by the iso-propylmercaptide ions and a t the range of pH 6.0-11.0 two reactions are competitive. Consequently, it is found that the observed data are satisfactorily accorded with the theoretical values over various pH range.

양로원과 요양원 간호사의 업무내용 비교 : 전국의 무료 양로 · 요양시설 중심으로

성기월,신임희,이경희,권경희 노인간호학회 2005 노인간호학회지 Vol.7 No.1

Purpose: The study was performed to compare the content of their jobs of the nurses working in the free elderly home with free nursing home in the whole country. Method: The subjects consisted of 138 nurses working in elderly home and nursing home. The data was collected from Jan. 1st to Feb. 22th 2005 using a structured questionnaire that included general characteristics, content of tasks which the nurse dose every day, health program, perceived job satisfaction and pay satisfaction scale. The collected data was analyzed by the SPSS(version 11.5) program including descriptive statistics, x^(2)-test. Result: On the nurses in the free elderly homes and nursing homes, there were many patient diagnosing hypertension, arthritis and dementia. Recently the death rate in elderly home and nursing home was highly increased. The mean period of nurses working in elderly home and nursing home were 3.75 years and 2.76 years. Experience of staying with the elderly was 70.9% on nurses working in elderly home. and 73.2% on nurses working in nursing home. Daily allotment of the nurses working in elderly home and nursing home were health assessment, medication care and eating care. The highest priority rank of nursing care of the nurse working in elderly home and nursing home was health assessment. Mean number of the patient in daily nursing care of the nurse residing in elderly home and nursing home was referral to hospital on 1-5persons/day and then transfer care on 1-6persons/day. The highest health care program was an exercise program. Conclusion: The resident nurses in the free elderly homes and nursing homes have had various types of tasks, and for their security a system for job satisfaction was needed.