간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

Phonology-Morphology Interaction in Lexical Phonology

Kim, Ki Hwa 대한언어학회 1995 언어학 Vol.3 No.-

Kim, Ki-Hwa(1995).Phonology-Morphology Interaction in Lexical Phonology. Linguistics 3. In this paper a theory of phonology-morphology interaction in Lexical Phonology is proposed. This theory distinguishes two types of phonology in relation to phonology-morphology interaction: cyclic phonology and noncyclic phonology. It is argued that clear distinction between cyclic and noncyclic phonological rules exists and that the domain of noncyclic phonology, different from that of cyclic phonology, is not confined to the lexicon. The validity of the arguments is examined through the analyses of Korean.

Consonant Cluster Simplification in Korean English

Kim, Ki Hwa 대한언어학회 2000 언어학 Vol.8 No.1

Kim, Ki-Hwa. 2000. Consonant Cluster Simplification in Korean English. Linguistics 8-1, 275-295. The purpose of this paper is to examine within the OT framework whether it is possible to predict the CCS of Korean English. The analysis in rule-and-derivation phonology shows that the CCS of Korean English is characterized by extra-vowel insertion regardless of its syllabic constituency, which leads to the assumption that the CCS is the result of filtering English in terms of Korean phonology and of the consonant cluster being simplified. Such assumption enables prediction on the CCS of Korean English within the OT framework, as follows. The CCS, the Korean version of English, is an error pattern caused by assuming different constraint ranking from that of English, and also the result of a transfer of Korean phonology by assuming the Korean language specific constraints. (Sohae College)

A Project Work on English Pronunciation

Kim, Ki Hwa 대한언어학회 1999 언어학 Vol.7 No.3

Kim, Ki-Hwa. 1999. A Project Work on English Pronunciation. Linguistics 7-3, 321-339. The purpose of this paper is to present a teaching model for English pronunciation. This model is designed to develop the student's English pronunciation skill with an emphasis on the communicative interaction. The first part of this paper examines major affecting factors on the acquisition of English pronunciation, where the importance of the teacher's role is emphasized, and then proceeds to discuss the conditions of good class management The second part of this paper discusses why an integrated approach is preferred to a linear approach and suggests a project work on English pronunciation which can be characterized as 'heuristic, student-centered, communication-centered'. (Sohae College)

알쯔하이머형 치매와 혈관성 치매환자에서의 ALDH2와 APOE 유전자의 다형성에 관한 연구

김동홍,김영돈,이창화,엄기춘,김은식,윤경식,김동희 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.2

연구목적: 본 연구는 국내 치매환자들을 대상으로 치매발병의 위험요소가 될 수있는 ALDH2와 APOE 유전자형이 어떤 양상으로 나타나는지 알아보기 위하여 시행되었다. 방 법: 충남 부여군에 위치한 부여노인병원에 입원중인 65세 이상의 알쯔하이머형 치매와 혈관성 또는 혼재성 치매환자 59명(남:19, 녀:40)을 대상으로 ALDH2와 APOE 유전자형을 제한효소법에 의하여 조사하였고 치매유형에 따른 유전자형과 대립유전자의 빈도를 비교하였다. 결 과: 1) 알쯔하이머형 치매환자군에서 혈관성 또는 혼재성 치매환자군보다 ALDH2*(-/2)유전자형의 빈도가 높았으나 통계학적으로 의미있는 차이는 없었다. 2) 알쯔하이머형 치매화자군에서 혈관성 또는 혼재성 치매환자군보다 APOE*(-/ε4) 유전자형의 빈도가 높았으나 통계학적으로 의미있는 차이는 없었다. 3) APOE*ε4와 ALDH2*2를 알쯔하이머병의 균등한 위험대립유전자로 가정하고 각 유전자형의 대립유전자의 수를 점수화하여 각 치매환자군의 위험유전자 전체점수를 비교한 결과 알쯔하이머형 치매환자군에서 점수가 더 높게 나왔으나 통계학적으로 의미있는 차이는 없었다. 결 론: 알쯔하이머형 치매환자군에서 혈관성 또는 혼재성치매치매환자군보다 ALDH2*2와 APOE*ε4 대립유전자의 빈도가 높게 나왔으나 통계학적으로 의미있는 차이는 없었다. A study on gene polymorphism of ALDH2 and APOE in patients with alzheimer's disease and vascular or mixed dementia. Objective : This study was designed to investigate gene polymorphism of ALDH2 and APOE, reported the risk factor of Alzheimer's disease, in korean dementia patients. Method : 59 Patients(male:19, female:40) with dementia, aged 65 and older in Buyeo geriatric hospital located in Buyeo-gun, chungnam, were finally included in this study. gene polymorphism of ALDH2 and APOE was typed with polymerase chain reaction in patients with Alzheimer's desease and vascular or mixed dementia. Results : 1) There was no difference in allelic frequency of ALDH2 gene polymorphism between patients with Alzheimer's disease and vascular or mixed dementia. 2) There was no difference in allelic frequency of APOE gene polymorphism between patients with Alzheimer's disease and vascular or mixed dementia. 3) Supposing APOE*ε4 and ALDH2*2 were equal risk allele of Alzheimer's disease, the sum of score by counting each risk allele was higher in patients with Alzheimer's disease than vascular mixed dementia, however there was no significant difference. Conclusion : The genotype frequency of ALDH2*(-/2) and APOE*(-/ε4) was higher in patients with Alsheimer's disease than vascular or mixed dementia, however there was no significant difference in allelic frequency of gene polymorphism of ALDH2 and APOE between patients with Alzheimer's disease and vascular or mixed dementia.

LMA를 활용한 용산삼개덜머리집 분석

김기화 한국무용연구회 2008 한국무용연구 Vol.26 No.2

This study aims to analyze `Yongsansamgaedeolmeorigip` through LMA(Laban Movement Analysis). To examine the performance, this paper consists of 6 chapters; Body, Effort, Shape, and Space are important instruments in studying the meanings of it. With each scene having conflict or interaction, it makes the climax and comes to its very end. The scene of seduction in the 2nd and 4th chapter employs Proximal Initiation and main movement applied in Rotation shows the voluptuous beauty of woman. Drive is emphasized, by combining Vision Drive with Rotation: use of Passion Drive and immersion of woman`s voluptuous charms makes an easier access to the 5th chapter. Connection structure of Initiation and Simultaneous, Crosslateral pattern of body, and combination of Action Drive lead into the climax. The union of Shaping(Gathering) and Passion Drive represented in the scene of taking off red-colored hansam, a symbol of Yongsansamgaedeolmeorigip, is not as appealing as the early parts. To get a maximal expression, I try to make up the established dance system through Vision Drive Effort and Spoke-like Directional. 본 연구에서는 루돌프 폰 라반(Rudolf von Laban: 1897-1958)의 움직임 분석체계인 LMA를 통해 본 연구자 안무의 1인 무용작품 [용산삼개덜머리집]을 분석하였다. [용산삼개덜머리집]은 분석을 위해 전체 6개의 장면으로 나누어 LMA, 즉 라반움직임분석(Laban Movement Analisis) 이론체계인 신체(Body), 에포트(Effort), 형태(Shape), 공간(Space) 요인을 통해 춤의 내면에 표현되어있는 작품의 의미를 분석하였다. [용산삼개덜머리집]은 각 장면이 서로 다르게 혹은 보완적으로 작용하며 클라이막스를 만들고 이로써 대단원을 갖는 극성(劇性)을 보여주고 있다. 특히 기법적인 면에서 2장과 4장의 Rotation의 기법으로 여성의 관능미를 보여 주고 있다. Drive 역시 2장의 조심스럽게 상대를 응시하는 Vision Drive를 Rotation에 결합하여 강조하였고 공간이 빠진 Passion Drive를 이용하여 자기중심적으로 신체에 몰입하며 만드는 여성의 육체적인 몰입을 통해 뒤이어지는 5장의 갈등으로의 전환을 용이하게 이끌었다. 5장 Distal Initiation과 Simultaneous의 연결구조, Crosslateral한 신체 활용패턴, Action Drive의 결합구조는 갈등적인 격정으로 이끌어지기에 충분하였다. 그러나 5장에서 애첩 용산삼개덜머리집을 상징하는 붉은색 꽃 형태의 한삼을 벗는 장면의 Shaphing(Gathering), Passion Drive의 결합은 앞의 갈등구조를 마무리 짓기에는 너무나 미온적인 에포트와 움직임을 결합하였다.

점성토의 침식에 관한 실험적 연구

김기철,이상화 東亞大學校 海洋資源硏究所 1988 硏究論文集 Vol.1 No.1

Laboratary experiments were carried out for the cohesive sediment sampled in the Keum River Estuary. Flume test was carried out in 12m long and 0.5m wide slope adjustable channel for erosion and deposition of the cohesive sediment. The critical shear stress and the coefficient for erosion were determined. The experimental results for cohesive sediment have not been reported in Korea. Thus, experimental results were compared with the other foreign results and showed fairly well agreement with them. The effect of salinity was neglected in the flume test. The results of flume test may be available to predict the cohesive sediment transport in the Keum River Estuary.

교정환자의 브라켓과 치아 경계부에 존재하는 치면세균막내 mutans streptococci 종 및 생물형의 식별

김미애,유소영,김화숙,국중기,임성훈,윤영주,김광원 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.1

본 연구는 교정환자의 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 치아 평활면의 치면세근막에 존재하는 mutaus streptococci의 종 및 생물형에 차이가 있는지를 알아보고자 시행되었다. 조선대학교 치과병원에 내원한 13세 이상 35세 미만의 환자 28명으로부터 브라켓을 장착하고 있는 61개 치아에서 치균세균막을 채취하여 mutans streptococci를 MSB 배지에서 선택적으로 분리한 다음, 이들의 지놈 DNA를 추출하여 dextranase 유전자를 표적으로 하는 중합효소연쇄반응법을 시행하고, 그 증폭물을 Hae Ⅲ로 소화하고, 이를 전기영동하여 제한효소절편길이에 따라 그 종을 식별하였다. 또한 생물형을 조사하기 위하여 생화학적 검사를 실시하였다. 그 결과 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 평활면의 치면세균막에 존재하는 mutans streptococci 종은 서로 비슷한 검출 빈도를 보이나 두 곳에 존재하는 mutans streptococci 생물형은 서로 차이가 있는 것으로 나타났다. 향후 브라켓과 치아 경계부 및 치아 평활면의 치면세균막의 mutans streptococci 생물형의 차이와 브라켓 주위의 법랑질 탈회 및 치아우식증 발병과의 상관관계에 대한 연구가 필요하다. The aim of this study was to compare the species and biotypes of mutans streptococci isolated from dental plaques sampled from the interfaces between the bracket and tooth surface and smooth tooth surfaces in orthodontic patients. Dental plaque was collected from the interfaces between brackets and teeth (test group), and from smooth tooth surfaces distant from brackets by more than 2 mm (control group). The dental plaque collected by a sterilized curette was transferred into a vial of 1 X PBS. The sample in the vial was vigorously vortexed for1 min and plated on mitissalivarius bacitracin (MSB) agar plate using cotton tips. The agar plates were incubated at 37℃ in a candle jar for 2 days, and again incubated for 1 more day at anambient temperature. Individual colonies were cultured in TH broth at 37℃ CO₂ incubator. The PCR-RFLP based on dextranase gene was performed for the identification of mutans streptococci at the species-level. For biotyping of mutans streptococci, biochemical tests were performed. There was no significant difference of the species of mutans streptococci isolated from both test and control groups. However, the biotypes of the mutans streptococci isolated from test and control groups were different. These results may offer the basic data to verify the relationship between the mutans streptococci biotype and enamel decalcification or dental caries in orthodontic patients with fixed appliances.

한국 사제 양성 지침서(2001년 7월 10일 승인)에 따른 서울대교구 대신학교 신학원 영적 양성

김기화 가톨릭대학교 사목연구소 2002 司牧硏究 Vol.10 No.-

가톨릭 전통 기도

김기화 가톨릭대학교 사목연구소 2003 司牧硏究 Vol.11 No.-