IVC systems: safety and effectiveness

Kevin Johnson 한국실험동물학회 2012 한국실험동물학회 학술발표대회 논문집 Vol.2012 No.8

Canine Model for Selective and Superselective Cerebral Intra-Arterial Therapy Testing

Camstra Kevin M.,Srinivasan Visish M.,Collins Dalis,Chen Stephen,Kan Peter,Johnson Jeremiah 대한신경중재치료의학회 2020 Neurointervention Vol.15 No.3

Purpose: With advancing endovascular technology and increasing interest in minimally invasive intra-arterial therapies such as stem cell and chemotherapy for cerebral disease, the establishment of a translational model with cerebral circulation accessible to microcatheters is needed. We report our experience catheterizing canine cerebral circulation with microcatheters, present high-resolution angiographic images of the canine vascular anatomy, describe arterial branch flow patterns and provide measurements of canine arterial conduits.Materials and Methods: Angiograms were performed on 10 intact purpose-bred hounds. Angiography, measurements of arterial conduits and catheterization information for intracranial arterial branches were obtained.Results: Selective and superselective cerebral angiography was successful in all subjects. Relevant arterial mean diameters include the femoral (4.64 mm), aorta (9.38 mm), external carotid (3.65 mm), internal carotid arteries (1.6 mm), vertebrobasilar system and Circle of Willis branches. Catheterization of the Circle of Willis was achieved via the posterior circulation in all subjects tested (n=3) and the use of flow directed microcatheters resulted in reduced arterial tree deformation and improved superselection of intracranial vessels. Catheterization of the intracranial circulation was attempted but not achieved via the internal carotid artery (n=7) due to its tortuosity and subsequent catheter related vasospasm.Conclusion: The canine cerebral vasculature is posterior circulation dominant. Anterior circulation angiography is achievable via the internal carotid artery, but direct cerebral arterial access is best achieved via the posterior circulation using flow-directed microcatheters. It is feasible to deliver intra-arterial therapies to selective vascular territories within the canine cerebral circulation, thus making it a viable animal model for testing novel intra-arterial cerebral treatments.

Gamification and Validation of an Upper Extremity Passive Machine for a Robot-Assisted Stroke Rehabilitation Gym

Sheila Saberry,Kevin Bui,Michelle J. Johnson 한국재활복지공학회 2018 한국재활복지공학회 학술대회논문집 Vol.2018 No.11

Comparison of knee valgus motion in African American male and female collegiate athletes

( Timothy W. Hughley ),( Kevin R. Ford ),( Gregory D. Myer ),( Joyce Johnson ),( Aram Yoon ) 국민체육진흥공단 한국스포츠정책과학원 2019 International Journal of Applied Sports Sciences Vol.31 No.1

Knee joint valgus is often implicated as a hazardous position for the anterior cruciate ligament (ACL) and has been linked to ACL injury risk. Although several groups have previously examined racially diverse cohorts to determine if there are predispositions for injury, there is little evidence found that explore sex differences in valgus angles in African American athletes. The purpose of this study was to identify the difference of the knee valgus angles between African American male (AAM) and female (AAF) student-athletes. Eighty-five National Collegiate Athletic Association, Division II African American male (n = 36) and female (n = 49) student-athletes participated in this study. Subjects dropped directly down off a box and immediately performed a maximum vertical jump. Valgus and flexion angles were analyzed and calculated based on the relationship of the tangent for each image. Knee valgus angles were significantly different between AAM and AAF athletes both at initial contact and maximum valgus displacement (p < .05). Significant differences in knee flexion angle was observed with the AAM having greater Initial Contact knee flexion (p < .05). The current data indicates that sex differences, specifically increased knee valgus angles, exist during jumping and landing. Neuromuscular training programs should be designed to specifically address excessive valgus knee motion and improve landing knee flexion among these athletes.

Inhibitory Potential of Tea Polyphenolics and Influence of Extraction Time Against Helicobacter pylori and Lack of Inhibition of Beneficial Lactic Acid Bacteria

Chandrakant Ankolekar,David Johnson,Marcia da Silva Pinto,Kevin Johnson,Ronald Labbe,Kalidas Shetty 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.11

Tea polyphenolics such as catechins are known to have the potential to inhibit many bacterial pathogens. Helicobacter pylori has been identified as an etiologic agent in the development of gastric ulcer, peptic ulcer, gastritis, and many other stomach-related diseases. In this study, we investigated the effect of 9 tea extracts—3 different brands representing 4 different processed types (white, green, oolong, and black)—on the inhibition of H. pylori. Extraction times of 2 and 5 minutes were compared. Most 5-minute extracts showed H. pylori inhibition, whereas 2-minute extracts only of Choice darjeeling black and Tazo white showed inhibition. No recovery was observed after the addition of 0.5 and 5 mM proline, indicating that tea polyphenols do not inhibit H. pylori by inhibition of proline oxidation via proline dehydrogenase. Extracts that showed inhibition were further evaluated for their effect on beneficial lactic acid bacteria. None of the samples showed inhibition, suggesting that tea might be able to inhibit H. pylori without affecting the beneficial lactic acid bacteria. High-performance liquid chromatography indicated the presence of gallic acid, quercetin, caffeine, and tea catechins (including catechin, epicatechin, and epigallocatechin) in all the tea samples. Our study indicates that tea can be potentially used as a low-cost dietary support to combat H. pylori–linked gastric diseases without affecting the beneficial intestinal bacteria.

The Effect of an 8 Week Neuromuscular Training Program on Knee Movement Biomechanics in African American Female Athletes

( Timothy W. Hughley ),( Joyce Johnson ),( Kevin R. Ford ),( Aram Yoon ) 한국스포츠정책과학원 2021 International Journal of Applied Sports Sciences Vol.33 No.2

This study examined the effects of an eight-week neuromuscular training (NMT) program on knee valgus angle in African American female athletes. Twenty-six female collegiate athletes participated. NMT group (n=15, 19.6±1.12 years) underwent an intervention training program that included three main components (plyometric and movement, core strengthening and balance, and resistance training). While, control group (n=11, 19.3±1.50 years) underwent the resistance training protocol for eight weeks. We hypothesized the NMT program would significantly decrease knee valgus angles during a drop vertical jump (DVJ) at landing for the NMT group when compared to the control group. Wilcoxon signed rank tests were done on the Pre-and Post-test findings. Results showed maximum valgus angle (VGmax) is significantly decreased (p<.05), and Maximum flexion (Flexmax) is significantly increased during the drop vertical jump in dominant leg (p<.05). The results support the hypothesis that an 8-week NMT program that combines injury prevention-training components can decrease an injury risk factor such as knee valgus in African American female athletes.

Anti-Hyperglycemia Properties of Tea (Camellia sinensis) Bioactives Using In Vitro Assay Models and Influence of Extraction Time

Chandrakant Ankolekar,Trisha Terry,Kevin Johnson,David Johnson,Ana Christina Lopez Barbosa,Kalidas Shetty 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.10

Tea (Camellia sinensis) has well-known health benefits, which are attributed to its polyphenolic metabolites. This research explored the potential of regular tea consumption and influence of extraction time typically used in daily consumption of tea, as a therapeutic dietary support for potential management of early stage type 2 diabetes using in vitro assay models. Extraction times of 2 and 5 minutes were compared. The 5-minute extraction time had significantly higher total phenolic content compared with the 2-minute extraction time. Choice Darjeeling 5-minute extraction yielded the highest amount of total phenolics (299.6±5.9 mg/g), followed by Tazo Black 5-minute extraction (240±9.7 mg/g), whereas Bigelow Green 2-minute extraction had the lowest total phenolic content (53±8.2 mg/g). 1,1-Diphenyl-2-picrylhydrazyl scavenging-linked antioxidant activity was high (81–91%) for all types evaluated, and for most samples it was influenced by the extraction time. Similarly, high in vitro α-glucosidase inhibition was observed in almost all the samples assayed, and for most samples the 5-minute extraction had significantly higher inhibition compared with the 2-minute extraction time. The most fermented teas showed highest α-amylase inhibition: Choice Darjeeling 5-minute extraction had the highest inhibition (84.1%), followed by Tazo Black 5-minute extraction (71.6%). Angiotensin converting enzyme inhibition was not observed in any sample. Overall, the 5-minute extraction time was found to have more relevance for potential benefits for managing hyperglycemia than the 2-minute procedure. This research suggests that tea offers an attractive potential strategy to regulate postprandial hyperglycemia toward an overall dietary support for type 2 diabetes management.

Identification of trehalose as a compatible solute in different species of acidophilic bacteria

Pedro A. Galleguillos,Barry M. Grail,Kevin B. Hallberg,Cecilia S. Demergasso,D. Barrie Johnson 한국미생물학회 2018 The journal of microbiology Vol.56 No.10

The major industrial heap bioleaching processes are located in desert regions (mainly Chile and Australia) where fresh water is scarce and the use of resources with low water activity becomes an attractive alternative. However, in spite of the importance of the microbial populations involved in these processes, little is known about their response or adaptation to osmotic stress. In order to investigate the response to osmotic stress in these microorganisms, six species of acidophilic bacteria were grown at elevated osmotic strength in liquid media, and the compatible solutes synthesised were identified using ion chromatography and MALDI-TOF mass spectrometry. Trehalose was identified as one of, or the sole, compatible solute in all species and strains, apart from Acidithiobacillus thiooxidans where glucose and proline levels increased at elevated osmotic potentials. Several other potential compatible solutes were tentatively identified by MALDITOF analysis. The same compatible solutes were produced by these bacteria regardless of the salt used to produce the osmotic stress. The results correlate with data from sequenced genomes which confirm that many chemolithotrophic and heterotrophic acidophiles possess genes for trehalose synthesis. This is the first report to identify and quantify compatible solutes in acidophilic bacteria that have important roles in biomining technologies.

Palliative Radiotherapy in the Presence of Well-Controlled Metastatic Disease after Initial Chemotherapy May Prolong Survival in Patients with Metastatic Esophageal and Gastric Cancer

Mohan Hingorani,Sanjay Dixit,Miriam Johnson,Victoria Plested,Kevin Alty,Peter Colley,Andrew W. Beavis,Rajarshi Roy,Anthony Maraveyas 대한암학회 2015 Cancer Research and Treatment Vol.47 No.4

Purpose We report the outcomes of patients treated with palliative radiotherapy (pRT) to the primarytumour in the context of well-controlled metastatic disease after initial chemotherapy. Materials and MethodsClinical records of 132 patients with metastatic esophago-gastric (OG) cancer treated withpalliative chemotherapy (pCT) between January 2009 and June 2013 were reviewed. Ninetysevenpatients had responding or stable disease after 3 months of chemotherapy, of whom53 patients received pRT to the primary tumour after initial chemotherapy in the presenceof well-controlled metastatic disease (group A, pCT-RT). The remaining 44 patients weretreated with pCT alone (group B, pCT). Treatment-related outcomes were assessed in abovegroups including time to local progression (TTLP), progression-free and overall survival. ResultsThe median overall survival for patients treated with pRT after initial chemotherapy (groupA) was 23.3 months (95% confidence interval [CI], 17.70 to 28.89 months) and significantlyhigher than the 14 months (95% CI, 10.91 to 17.08 months) in patients treated with pCTalone (group B) (p < 0.001). The use of pCT-RT was an independent predictor of OS in multivariateanalysis. Local recurrence was observed in 12/53 of patients (23%) in group Acompared to 16/44 (36%) in group B. The median TTLP was significantly higher in patientsafter pCT-RT at 17.3 months (5.23 months to 44.50 months) compared to 8.3 months(range, 4.10 to 25.23 months) in patients treated with pCT alone (p=0.006). ConclusionThe possibility of pRT influencing systemic disease in advanced OG cancer has not beenreported, and results from the present study present strong arguments for investigation ofthis therapeutic strategy in a randomized trial.

Learners’ Responses to a Virtual Cadaver Dissection Nerve Course in the COVID Era: A Survey Study

Jeffrey L. Lisiecki,Shepard Peir Johnson,David Grant,Kevin C. Chung 대한성형외과학회 2022 Archives of Plastic Surgery Vol.49 No.5

Background Virtual education is an evolving method for teaching medical learners. During the coronavirus disease 2019 pandemic, remote learning has provided a replacement for conferences, lectures, and meetings, but has not been described as a method for conducting a cadaver dissection. We aim to demonstrate how learners perceive a virtual cadaver dissection as an alternative to live dissection. Methods A virtual cadaver dissection was performed to demonstrate several upper extremity nerve procedures. These procedures were livestreamed as part of an educational event with multimedia and interactive audience questions. Participants were queried both during and after the session regarding their perceptions of this teaching modality. Results Attendance of a virtual dissection held for three plastic surgery training institutions began at 100 and finished with 70 participants. Intrasession response rates from the audience varied between 68 and 75%, of which 75% strongly agreed that they were satisfied with the virtual environment. The audience strongly agreed or agreed that the addition ofmultimedia captions (88%), magnified video loupe views (82%), and split-screen multicast view (64%) was beneficial. Postsession response rate was 27%, and generally reflected a positive perspective about the content of the session. Conclusions Virtual cadaver dissection is an effective modality for teaching surgical procedures and can be enhanced through technologies such as video loupes and multiple camera perspectives. The audience viewed the virtual cadaver dissection as a beneficial adjunct to surgical education. This format may also make in-person cadaver coursesmore effective by improving visualization and allowing for anatomic references to be displayed synchronously.