DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

다기관 기능부전을 초래한 치명적인 삼일열 말라리아 증례

박상원,김동우,박주원,이순일,신영학,김의종,오명돈,최강원 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

삼일열 말라리아는 국내에서 재유행하고 있는 감염질환으로, 일반적으로 양성의 임상경과를 거친다. 저자들은 특이한 질병력이 없이 건강하였던 젊은 성인이 국내에서 삼일열 말라리아에 걸린 후 다기관기능부전과 폐출혈으로 사망한 증례를 경험하였다. 환자의 중증 임상경과를 설명할 만한 특이한 인자가 발견되지 않았다. Vivax malaria is a re-emerging infectious disease in Korea with cumulating 19,416 cases as of December 2003 since 1993. Vivax malaria is generally a benign disease with few severe complications. Even in the worldwide literature, there are only small number of case reports on severe complications. We experienced a fatal vivax malaria in a previously healthy young adult with severe multi-organ failure and fatal massive pulmonary hemorrhage. The patient presented with abdominal pain and shock. There were splenic hemorrhage, disseminated intravascular coagulation, oliguric acute renal failure requiring dialysis, and pulmonary hemorrhage leading the patient finally to death. The initial parasitemia was 2,046/μL. Thin smear and PCR revealed only Plasmodium Vivax. There was no explainable cause of death except for the only serological suggestion of Mycoplasma pneumoniae infection without correlating clinical evidence. This is a first case of fatal vivax malaria in Korea since its re-emergence in 1993. Further case monitoring is needed to define whether this is a index case or an isolated one.

Investigation of the April 2000 forest fire damage in Kang-won Province, Korea, using RADARSAT data

Kang, Sung Chul,Seo, Jin Won,Lee, Tae Hee,Park, Sang Eun,Hong, Suk Young,Moon, Wooil M. 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1

The series of forest Fires in Kangwon Province in April, 2000, were one of the mast serious ones in the Kang-won Province in recent years. The fire damaged areas are extensive and this paper investigates the utility of the satellite image data, particularly the RADARSAT SAR data. One of the main objectives of this study included evaluation of potential usefulness of the C-band RADARSAT data in the detection of forest fires in mountainous areas. Among several fire damaged sites, the area near Samchuk (city) was selected as the main study area and mufti-temporal RADARSAT(raw) data were acquired and the processed results were comparatively investigated with the IRS-1c data acquired shortly after the fire. To identify the burnt area from the undamaged background in the optical imagery, supervised classification and NDVI processing were carried out and tested with the IRS-1C data. For this experiment, several sets of raw RADARSAT standard and Fine mode (S4, F3, ascending orbit) data were processed and the final image was formed. In addition to the final image, sigma nought (σ°) was catnputed and compared for the burnt area against the undamaged areas. Because of the steep slopes in the study area and subsequent deviation from the flat surface scattering models, the fire scars in the sigma nought (σ°) image were not apparent. However, fire scars are slightly detected in the difference and ratio images obtained from the signal intensity data acquired at different dates (bath before and after fire). Texture analysis of the image data including the burnt areas were carried out using the Multiplicative Markov Random-Field (MMRF) algorithm. The detection of the fire damaged area from both standard mode and fine mode RADARSAT was not as straightforward as in optical data. However, Previous studies with ERS-1 S_AR and RADARSAT data in Indonesia and in the North American Plains indicate that C-band SAR data provide us with an effective tool for studying the forest fire damages. However, the application of C-band SAR data to the analysis of the forest fire areas requires careful processing of the data with realistic scattering models.

관해유도 항암요법을 받는 백혈병 환자에서 진균 감염증의 예방 : 무작위 배정법과 이중 눈가림법에 의한 Fluconazole과 Nystain의 효과에 관한 다기관 공동연구 A Randomized, Double-blind, Multicenter Trial to Compare Fluconazole with Nystatin

최강원,오명돈,배현주,백경란,박선양,김병국,신완식,강문원,진종률,박종원,김춘추,김동집,한지숙,민유홍,이선주,고윤웅 대한화학요법학회 1993 대한화학요법학회지 Vol.11 No.2

Fluconazole의 진균 감염증 예방 효과와 안전성에 관하여 3개 대학병원에서 관해유도화학요법을 받는 급성 백혈병환자를 대상으로 무작위 배정법과 너도나도 누가림법에 의하여 연구하였다. 모두 62명의 환자에게 fluconazole(100㎎ bid) 또는 nystatin(1,000,000IU/day)을 무작위로 투여하였다. 투약은 관해유도화학요법과 같은 날짜에 시작하여 호중구수가 1,000㎣이상으로 회복되거나 진균 감염증이 확인되거나 의심되어 Amphotericin-B를 시작하거나, 약과 관련된 부작용이 나타날 때까지 계속하였다. 진균 colonization은 fluconazole군에서 감소하였으나 nystqatin군에서는 증가하였다, 표재성 진균감염증으로 nystatin군에서 C. albicans 진균혈증 1례와 C.parasilosis 진균혈중 1례가 발생하였다. 경험적 항진균요법으로 Amphotericin-B를 투여한 경우는 fluconazolerns 34명중 7례(21%), nystatinrns 28명중 10례(36%)였다(p<0.05). Fluconazole군과 nystatin군 사이에 부작용이나 사망률에 차이는 없었다. 결론적으로, fluconazole은 관해유도화학요법을 받는 급성 백혈병환자에서 진균의 colonization을 줄이는데 효과적이고 안전한 항진균제이다. We made a randomized, double-blind, multicenter trial to compare the efficacy and safety of fluconazole with nystatin for prevention of fungal infections in patients with acute leukemia. Sixty-two adult undergoing remission induction chemotherapy for cute leukemia were enrolled. Patients were randomly assigned to receive either fluconazole (100㎎ bid) or nystatin(1,000,000IU×6/day) with corresponding placebo. The study drug was started in initiation of chemotherapy and continued until recovery of neutrophil counts(>1,000/㎣), development of proven or suspected invasive fungal infection, or the occurrence of drug-related toxicity. Fungal colonization decreased in fluconazole(F) group, however increased in nystain(n) group. Superficial fungal infection occurred in 1 of 34 F group, whereas invasive fungal infection developed in 3 of 28 N group. Empirical amphotericin-B therapy was given in 7 of 34(21%) F group and 10 of 28(36%) N group(p>0.05). The incidence of drug-related side effects and overall moratlity were similar in both study groups.

심장혈관용 약물코팅풍선카테터의 개발 동향

박정훈 ( Junghun Park ),조원일 ( Won-il Jo ),변대흥 ( Dae-heung Byeon ),강소영 ( So Young Kang ),남충현 ( Choong-hyun Nam ),서경우 ( Kyoung-woo Seo ),박준규 ( Jun-kyu Park ) 한국공업화학회 2022 공업화학 Vol.33 No.5

급격한 고령화 사회로의 진입과 서구화된 식습관에 의해 협심증, 심근경색증, 고혈압 등에 의한 심장질환자의 수가 5년간 10만명 이상 증가했다. 심장질환은 지난 8년간 꾸준히 국내 사망원인 2위를 기록 중이며, 이로 인해 연간 소모되는 의료비는 6조원에 달한다. 증가한 환자와 함께 다양한 치료법이 제시되고 있는 가운데, 현재 보편화 된 관상동맥 중재시술 중 스텐트 삽입술의 단점인 재협착이나 장기간 이중항혈소판제 복용으로 인한 출혈의 위험 증가 등의 문제점을 보완하기 위해 약물코팅풍선카테터가 개발되어 점차 상용화되고 있는데, 국내에선 2010년 ‘SeQuent Please^Ⓡ (비브라운코리아)’라는 제품이 처음 출시되면서 약물코팅풍선카테터를 사용한 시술이 시작되었고, 이후 2012년 보험급여가 적용되면서 그 사용량이 본격적으로 늘어났다. 약물코팅풍선치료는 국내뿐만 아니라 전 세계적으로 사용이 증가하고 있는데, 특히 일본을 포함한 아시아태평양 지역에서 그 사용량이 급격히 증가하고 있다. 현재까지 국산 제품의 수요가 증가하고 있는 상황이며 향후, 동물시험 및 임상시험에서의 성능이 입증된다면 위험성이 높은 스텐트 삽입술에 비해 효과적인 시술이 될 것이라 예상한다. As a result of the rapid entry into an aging society and westernized eating habits, the number of heart disease patients caused by angina pectoris, myocardial infarction, and high blood pressure has increased by more than 100,000 over five years. Heart disease has consistently ranked second in the cause of death in Korea over the past eight years, and medical expenses consumed annually reach 6 trillion won. While various treatments are being proposed for more patients, drug-coated balloon catheters have been developed and gradually commercialized to solve the disadvantages of stent implantation such as restenosis and increased risk of bleeding due to long-term double antiplatelet medication. In Korea, it began to use a drug-coated balloon catheter with the first release of it called “SeQuent Please^Ⓡ (Bibrown Korea)” in 2010. Its demand increased gradually as insurance benefits were applied in 2012. Drug-coated balloon angioplasty is increasing in use not only in Korea but also around the world, especially in the Asia-Pacific region, including Japan. Until now, the demand for domestic products is increasing, and if the efficiency in vivo and clinical trials is proven in the future, it is expected to be an effective procedure compared to high-risk stent implantation.

황색포도구균의 균혈증에서 초기 항생제 치료가 예후에 미치는 영향

김성한,박완범,이기덕,강철인,최영주,김홍빈,박상원,김의석,오명돈,김의종,최강원 대한감염학회 2002 감염 Vol.34 No.5

우리나라 노인여성의 골다공증성 척추골절로 인한 경제적 부담 추계

강혜영,강대룡,장영화,박성은,최원정,문성환,양규현,Kang, Hye-Young,Kang, Dae-Ryong,Jang, Young-Hwa,Park, Sung-Eun,Choi, Won-Jung,Moon, Seong-Hwan,Yang, Kyu-Hyun 대한예방의학회 2008 예방의학회지 Vol.41 No.5

Objectives : To estimate the economic burden of osteoporotic vertebral fracture (VF) from a societal perspective. Methods : From 2002 to 2004, we identified all National Health Insurance claims records for women ${\geq}50$ years old with a diagnosis of VF. The first 6-months was defined as a "clearance period," such that patients were considered as incident cases if their first claim of fracture was recorded after June 30, 2002. We only included patients with ${\geq}$ one claim of a diagnosis of, or prescription for, osteoporosis over 3 years. For each patient, we cumulated the claims amount for the first visit and for the follow-up treatments for 1 year. The hospital charge data from 4 hospitals were investigated to measure the proportion of the non-covered services. Face-to-face interviews were conducted with 106 patients from the 4 study sites to measure the out-of-pocket spending outside of hospitals. Results : During 2.5 years, 131,453 VF patients were identified. The patients had an average of 3.38 visits, 0.40 admissions and 6.36 inpatient days. The per capita cost was 1,909,690 Won: 71.5% for direct medical costs, 20.6% for direct non-medical costs and 7.9% for indirect costs. The per capita cost increased with increasing age: 1,848,078 Won for those aged 50-64, 2,084,846 Won for 65-74, 2,129,530 Won for 75-84and 2,121,492 Won for those above 84. Conclusions : Exploring the economic burden of osteoporotic VF is expected to motivate to adopt effective treatment options for osteoporosis in order to prevent the incidence of fracture and the consequent costs.

필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

R_2O-MgO-MgF_2-SiO_2계 Glass의 Crystallization에 관한 연구

강원호,박병구 단국대학교 신소재기술연구소 1993 신소재 Vol.3 No.-

본 연구에서는 Fluour mica group^1)의 하나인 Lithium fluorhectorite mica (LiMg_2LiSi_4O_10F2) 결정상^2)을 얻기위해 Li_2O-MgO-MgO-MgF_2-SiO_2계를 기본 조성으로 선정하여Tdmaum 결성화시켰을때에 나타나는 벽개성(cleavage)의 원인을 mica의 결정구조 XMg_2LiSi_4O_10F2에서 interlayer cation에 있는 것으로 보고서 X에 해당하는 R_2O성분 LI_2O, Na_2O, K_2O를 4∼10 wt% 변화시켜 Pt-crucible을 사용하여 1450℃에서 모유리를 제조하였다. 제조된 모유리의 DTA, TMA 열분석 결과를 토대로, 결정화 열처리를 핵형성 610∼690℃, 결정성장 900∼1150℃, 각각 2시간, 4시간으로 결정화 sample을 얻었으며 XRD와 SEM을 사용하여 결정상과 미세 구조를 분석하였다. Tg는 520℃, Mg는 580℃였으며, 모유리 상태는 부분 유백의 모유리를 얻을 수 있었다. 최적 결정화 조건은 핵형성 630∼670, 결정성장 1100℃였으며 결정상은 Lithium (Sodium, Potassium) fluorhectorite 와 talc, 그리고 quartz였다. 벽개성이 가장 우수한 조성은 Li_2O 6 wt%, MgO 10.8 wt%, MgF_2 16.7 wt%, SiO_2 6.4 wt% 였다. In this study, the crystallization of R_2O-MgO-MgF_2-SiO_2 fluormica glass-ceramics were investigated. Lithium fluorhectorite mica (LiMg_2LiSi_4O_10F_2), one of the water swelling mica group is composed of Li_2-O-MgO-MgF_2-SiO_2 glass system and this composition added TiO_2, ZrO_2 and P_2O_5 as nucleating agent. The interlayer cation Li^+ of the composition was replaced by Na^+ -and K^+ -ions. this base glass was melted at 1450℃ per 1h. After heat-treatment on the bases of thermal analysis of this base glass by DTA and TMA, crystal phase and sizes upon to the nucleation and crystallized temperature were observed by XRD and SEM. When crystallized, the crystal phase is lithium fluorhectorite, talc and quartz. It is found that the crystal phase to influence self delamination is lithium fluorhectorite one. In the case of substitution of interiayer cation Na^+ and K^+ for Li^+, properties of cleavage and delamination was not found and also crystal phase containing Li^+ is different from one of that Na^+, K^+. The optimum chemical composition are LI_2O 6 wt%, MgO 10.8 wt%, MgF_2 16.7wt%, SiO_2 66.4 wt% and the optimum temperature for crystallization having excellent delamination is 1100℃ and nucleation for temperature is 630℃ -670℃. When used TiO_2 as a nucleating agent, it show excellent crystallization.

치환된 알킬요드화물 또는 크산테이트 화합물과 에틸 아크릴산 또는 메틸 비닐케톤과의 분자간 라디칼 탄소-탄소 결합형성반응

강석구,박영원,박천호,조일환,오원택 성균관대학교 1992 論文集 Vol.42 No.2

생리활성천연물인 lipoxin A, carpenter bee 페로몬, 뇌염모기 페로몬, exobrevicomin 등의 합성의 주요 키탈 중간체들을 치환된 요드화물 또는 크산테이트 화합물과 에틸아크릴산 또는 메틸비닐케톤과의 분자간 라디칼 탄소-탄소 형성 반응에 의해 합성하였다. The chiral building blocks for the synthesis of biologically active natural products such as lipoxin A, carpenter bee pheromone, mosquito ov-iposition pheromone, and exo-brevicomins were synthesized by intermol-ecular radical C-C bond formation of the appropriate substituted iodides or xanthates with ethyl acrylate or methyl vinyl ketone.