대장선암에서 p53 과 P-glycoprotein의 분포에 관한 연구

강상균,김의한,장인성,김대중,양승하 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Adenocarcinoma of colon and rectum express many oncogenes. The p53 acts as tumor suppressor in normal tissue but mutant forms are known as doing important role in carcinogenesis of many malignant tumors, such as colorectal cancer, gastric cancer, and breast cancer. P-glycoprotein shows distribution in many normal tissues and malignant tumor. This protein occurs multidrug resistance to therapeutic anticancer drug, resulting in poor prognosis of patients. The expression of p53 and P-glycoprotein has been studied by many authors according to clinicopthologic findings of colorectal adenocarcinoma, but these results are controversial yet. To observe the relationship between both antigen and pathologic findings, including prognostic factos, of colorectal adenocarcinoma, we analysed 13 cases adenoma and 62 cases adenocarcinoma then processed for immunohistochemical stain by SLAB method using mouse monoclonal anti-p53 and JSB-1. The results were as followings; 1) The expression rate of p53 in adenoma and adenocarcinoma are 38.5% and 71.0%, respectively, and P-glycoprotein are 46.6% and 58.1%, respectively. 2) The p53 revealed higher expression rate in case of well differnetiated form, invasion to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of moderately and poorly differentiated form, invasion to muscle, no lymphatic metastasis, but no difference accoding to site. 3) P-glycoprotein revealed higher positive rate in case of right side, invasion to surrounding adipose tissue, lymphatic metastasis compared to that of left side, invasion to muscle and serosa, no lymphatic metastasis, but no difference according to differentiation. 4) Both of p53 and P-glycoprotein revealed higher ex pression in case of right side, well and moderately differentiated form, invation to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of left side, poorly differentiated form, invasion to muscle, no lymphatic metastasis. Above results revealed that the expression of p53 begins in adenoma and shows close relation with development of colorectal carcinoma. p53 and P-glycoprotein may reveal the relation with poor prognosis. Adenocarcinoma showing both positive reaction of p53 and P-glycoprotein is also similar prognostic relation.

Numerical study of in-vessel retention under the gallium–water external reactor vessel cooling system using MARS-LMR

Kang, Sarah,Park, Seong Dae,Kim, In Guk,Bang, In Cheol Atomic Energy Society of Japan 2016 Journal of nuclear science and technology Vol.53 No.3

<P>To confirm the feasibility of the gallium-water IVR-ERVCS (in-vessel retention-external reactor vessel cooling system), this paper focuses on the numerical simulation of severe accidents in APR 1400 using MARS-LMR (multidimensional analysis of reactor safety-liquid metal reactor). To analyze the gallium-cooled systems, the properties of liquid gallium were added to the MARS-LMR code used in our previous work. In this system, the generated decay heat is transferred to liquid gallium through the reactor pressure vessel and then removed from the water pool as a heat sink. The numerical analyses results show that the temperature range of the liquid gallium is much lower than its boiling point and confirm the natural convection. Sensitivity studies were also performed by changing several parameters such as the initial temperature of gallium and water pool inventory and their results indicated that the working time of the gallium-water IVR-ERVCS depends on the inventory of the water pool. Because liquid gallium in this system does not have a phase change, unlike water, the gallium-water IVR-ERVCS can provide stable and reliable cooling capability. To solve the limitation due to critical heat flux in IVR-ERVCS and to ensure the sufficient thermal margin, it is confirmed that the gallium-water IVR-ERVCS can be a successful severe accident mitigation strategy in nuclear power plants.</P>

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

모델동물의 병태.생리 유발 유전자 기능 연구 : 당뇨모델동물의 췌장 β-세포를 이용한 비만발생에 관한 연구 Effect of development of diabetes by fatty acid in animal model

황대연,김용규,강태석,황진희,임채형,강현구,이주은,허영범,장인석,조정식,채갑용 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

체장 β세포에 지방이 축적되는 lopotoricity 현상은 인슬린 비의존성 당뇨 (Non InsuBin Dependent Diabetes Mellitus :NIDDM)의 발생과 상당히 밀접한 관계가 있으며, 췌장 β세포의 lipoapoptosis는 세포막형성에 관여하는 sphingomyelin의 전군체안 ceramide의 과생산에 의해 유도된다는 보고가 있다. 그러나, 지방산 종류에 따른 췌장 β세포에서의 세포사멸 (apoptosis)유발 기작에 대해서는 야직까지 보고된 바가 없파. 또한, 지방세포에서 생성되며, 지질대사, 음식섭취 및 체중조절에 관여하는 렙틴 (leptin)과 렙틴 수용체와의 관계 역시 체장 세포에서 명착하게 밝혀져 있지 않다. 따라서 본 연구에서는 6주령 53와 Zucker rat의 췌장으로부터 췌도를 분리하는 기술을 확립하고, 배양된 췌장 췌도 세포와 MS-1세포주에 불포차즌방산 (n-3 linolenic acid; n-6 :linoleic acid), 포화지방산 (stearic acid), conjugated linoleic acid (CLA)를 포함하는 4 가지의 지방산을 처리한 후 이들이 췌장 』세포의 사멸에 미치는 영향을 다양한 apoptosis 관련 유전자의 발현을 분석함으로써 확인하고자 하였다. 그 결과 SD rat으로부터 마리 당 평균 약 500개의 췌장 췌도를 분리하는데 성공하였으며, 4 종류의 지방산 중 포화지방산인 stearic acid를 처리하여 ONA fragmentation 증가를 관찰하였다. 또한 RT-PCR 방법을 이용하여 분리된 췌도 세포에사의 Bcl-2, Bax, iNOS가 지방산의 종류에 따라 서료 다른 발현양상을 나타낸다는 」라실을 관귿하였다. 이러한 떤구 결과는 지방산의 종류에 따라 췌장 췌도 세포에서의 세포사멸 유발 효과를 겋증하였으며, 이러한 연구결과는 복잡한 당뇨발병 원인 규명에 중요한 정보를 제공할 것으로 사료된다. Fat contents within the pancreatic islets are closely related to lipotoxicity which induces apoptosis, resulting in non insulin dependent mellituse(NIDDM). High level of fat may contribute to both insulin resistance and obesity in rodent and humans but the evidence is limited. To determine whether dietary fatty acids affect pancreatic cell function, the INS-1 cells and the pancreatic islets isolated from SD and Zucker obese(fa/fa) rats(6 wk, prediabetic stage) were cultured with stearic acid and CLA. As a result, DNA fragmentation laddering was substantially decreased in the INS-1 cells and the isolated pancreatic islets cultured with 2mM CLA compared with those with stearic aced. To investigate the mechanism by which CLA alleviates cell apoptosis as indicated by DNA fragmentation assay, we examined mRNA expressions of apoptosis-related proteins including Bax and Bcl-2 associated with cell death agonist and antagonist, respectively in the both INS-1 cells and islets cultured with 2 mM fatty acids. Bax mRNA expression was not markedly altered by either stearic acid or CLA, whereas Bcl-2 mRNA expression was noticeably enhanced by CLA compared with stearic acid. However, there were no changes in cell apoptosis and cell apoptotic-regulating gene products in the both INS-1 cells and isolated islets treated without or with 2 mM CLA. It is concluded that CLA may have a beneficial effect for maintenance of cell viability via increased Bcl-2 protein expression compared with steraic aced, which lead to delay or prevent, at least somewhat, the onset of NIDDM in the physiological status.

신생아 중증 세균성 감염에 대한 Augmentin(clavulanate potentiated amoxycillin)의 치료효과 및 안전성 연구

성인경,강진한,고대균,이경수 대한감염학회 1995 감염 Vol.27 No.1

Combination therapy of β-lactam antibiotics has been used widely to treat severe infections in neonates. The widespread use of β-lactam antibiotics has resulted in emergence of a broad variety of plasmid or chromosome mediated β-lactamases. Also, there is a great propensity for these enzymes to spread among various species of bacteria. Thus, the use of β-lactamase inhibitor antibiotics may be of great interest and has potential therapeutic value in severe neonatal infections. We tried to find out the efficacy and safety of clavulanate potentiated amoxycillin (Augmentin) in treatment of severe neonatal infections. Total thirty-five neonates with sepsis(26) or severe organ infections(9) were involved. Each 30mg/kg dose of Augmentin was injected at 12hr-or 8hr-or 6hr-intervals according to the age and weight of neonates. Clinically 77.1% of the cases were cured, and microbiologically 80.0% were eliminated. 88.9% of eighteen gram positive organisms(S. aureus, S. epidermidis, a -hemolytic strep., S. pneumoniae , E. faecalis) isolated from study group were susceptible to Augmentin. 47.0% of gram-negative organisms (K. pneumoniae, E.coli, P. mirabilis, S. marcescens, C. freundii) were suceptible. Thus, it was confirmed that Augmentin was very effective in ampicillin-or ceftriaxone-resistant gram positive cocci infections, but less effective in isolated K. pneumoniae and S.marcescens cases. The serum levels of amoxycillin in the neonates were maintined about 6㎍/ml until 1hr. at each injection. We observed that alkaline phosphatase level was elevated above 1,000IU/ℓ in three neonates and that skin rash developed after injection in one neonate. But, these side effects were mild and transient. These results suggest that Augmentin may be tolerable and effective in treating severe gram positive cocci infections in neonates.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

유지 투석중인 만성 신부전환자에서 허혈성 심질환의진단 지표로서의 심장트로닌-I의 유용성

신병철,강대웅,정지용,류봉관,서영욱,김정인,김범윤,김현리,정종훈 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background : Coronary disease is highly prevalent in patient with end stage renal disease (ESRD) and account for much of their observed morbidity and mortality. Troponin-I consistently maintains a high sensitivity and specificity and is most sensitive marker for ischemic heart disease (IHD). Method : We examed 49 hernodialyzed patients (22 male, 27 female) without evidence of acute coronary syndrome (ACS) for 6 months. Biochemical markers were measured in serial predialysis blood samples. For analysis, we used two cardio-specific assays for troponin-T (cTnT) as well as for troponin-I (cTnI) and compared the results with CK-MB (reference value ≤ 4.0 ng/mL) concentration. Results : Myocardial ischemia was observed in 47% (23/49) of patients. cTnT level above 0.1 ng/mL. and cTnI level above 0.5 ng/mL, were observed in 22% (11/49) and 20% (10/49) of patients respectively. cTnI revealed significantly higher positive rate in patients with myocardial ischemia than the patients without myocardial ischemia (43 % vs 30%) (p<0.05). cTnT and CK-MB revealed no difference in positive rate between the patients with and without myocardial ischemia (cTnT : 30% vs 15% and CK-MB : 30% vs 19%). The sensitivity and specificity of cTnI to myocardial ischemia were higher than those of cTnT and CK-MB (sensitivity 43% vs 30% and 30%. specificity 100% vs 85% and 81%). Conclusion: Both cTnT and cTnI are useful in ruling out myocardial injury in chronic renal failure patients. But. cTnI is a more sensitivity and excellent specificity of ischemic heart disease than cTnT and CK-MB in hemodialyzed patients. In patients with ischemic heart disease, the presences of DM and advanced age were higher than those in patients without ischemic heart disease (p<0.05). Among the baseline characteristics old age, elevated LDH and diabeties were significant more frequent in the patients with elevation of cardiac troponin-I (p>0.5 ng/mL) than those with cardiac troponin-I (p<0.5 ng/mL), p=0.038, p=0.049, and p=0.045, respectively. Our results suggest that these cTnI is the potential diagnostic marker for the prediction of IHD in ESRD patients.

노인 환자에서 경피적 관동맥 중재술 후 조영제 유발 신부전의 발생률, 위험인자와 임상적 의의

김광일,배장환,강현재,채인호,김효수,손대원,김철호,오병희,이명묵,박영배,최윤식 대한노인병학회 2004 대한노인병학회지 Vol.8 No.1

한국인에서 혈소판 당단백 Ⅱb/Ⅲa 유전자 다형성과 관동맥 성형술 후 재 협착과의 관계

이민수,이정우,김보영,임대승,강정아,김정희,김윤철,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Platelet aggregation is the final pathway of acute coronary syndrome such as acute myocardial infarction and unstable angina. Platelet glycoprotein IIb/IIIa is a membrane receptor for fibrinogen and yon Willebrand factor and it plays an important role in platelet aggregation and in the pathogenesis of acute coronary syndrome. It is known that polymorphism of the gene that encoding platelet glycoprotein IIb/IIIa(PI^A1/A2) is strongly related to acute coronary syndrome in Caucasian, but not in Koreans. We investigated relationship between platelet glycoprotein llb/Illa gene polymorphism and restenosis of coronary artery after angioplasty in Koreans. Total 371 patients(M=251. F=120) were enrolled. Angioplasty group comprised 143 patients who underwent coronary angioplasty, and in the angioplasty group, restenosis group comprised with the 65 patients who had restenotic lesion over 50% of luminal diameter in follow-up coronary angiography. Normal group comprised 153 patients who had no significant angiographic lesion and variant angina group comprised 75 patients who were positive in ergonovine test. Genomic DNA was extracted from peripheral arterial blood. To determine the frequency of P1^A1/A2 genotype, polymerase chain reaction(PCR) was done and the product was restricted with Mspl. 3%. agarrose gel electrophoresis showed restriction fragment length polymorphism. Clinical profile and risk factor were also reviewed. Among all 371 patients of study group, genotype of only one patients in restenosis group if is proven to be PI^A1/A2 heterozygote. All patients of normal study group, no restenosis group, and the other patients in restenosis group have an PI^A1 homozygote genotype. In our study, platelet glycoprotein IIb/Illa polymorphism has no relationship with restenosis of the coronary artery after angioplasty in Koreans. But the genotypic frequency of platelet glycoprotein IIb/IIIa gene polymorphism in Koreans is concordant with that of previous studies.