Protective Effects of Hyperoside against Carbon Tetrachloride-Induced Liver Damage in Mice

Choi, Jun-Ho,Kim, Dong-Wook,Yun, Nari,Choi, Jae-Sue,Islam, Md. Nurul,Kim, Yeong-Shik,Lee, Sun-Mee American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5

<P>In this study, the hepatoprotective effects of hyperoside (<B>1</B>), a flavonoid glycoside isolated from <I>Artemisia capillaris</I>, have been examined against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with vehicle or <B>1</B> (50, 100, and 200 mg·kg<SUP>−1</SUP>) 30 min before and 2 h after CCl<SUB>4</SUB> (20 μL·kg<SUP>−1</SUP>) injection. Levels of serum aminotransferases were increased 24 h after CCl<SUB>4</SUB> injection, and these increases were attenuated by <B>1</B>. Histological analysis showed that <B>1</B> prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl<SUB>4</SUB> treatment, and these changes were reduced by administration of <B>1</B>. Protein and mRNA expression of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl<SUB>4</SUB> injection. Compound <B>1</B> suppressed TNF-α, iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that <B>1</B> has protective effects against CCl<SUB>4</SUB>-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np200001x/production/images/medium/np-2011-00001x_0006.gif'></P>

골수염의 진단에 있어서의 Tc-99m Hexamethylpropylene amine oxime 백혈구 스캔의 임상적 유용성

최상호,김양수,정준원,정진원,추은주,서동대,배인규,양승오,우준희,류지소 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.1

배경 : 골수염의 진단에 있어 Technetium-99m을 이용한 삼상 골스캔(Tc-99m MDP스캔)은 단순방사선 검사상 정상소견을 보이는 경우에 쓰이는 1차적 검사로 매우 높은 민감도를 보인다. 그러나 Tc-99m MDP 스캔은 골절이나, 정형외과적 삽입물, 신경병적 관절병변과 같이 뼈의 재형성이 증가되는 상황에서도 양성소견을 보여 특이도가 낮은 단점이 있다. 이러한 단점을 보완하고자 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 유용성을 알아보고자 하였다. 대상 및 방법 : 골수염이 의심되어 Tc-99m HMPAO 백혈구 스캔을 시행했던 15명의 의무기록을 후향적으로 분석하였다. 골수염의 확진은 임상관찰 소견과 배양검사 결과와 병리소견을 기준으로 하였다. 결과 : 골수염의 진단에 있어 Tc-99m HMPAO 백혈구 스캔의 민감도와 특이도는 각각 100%와 78%였고 Tc-99m HMPAO 스캔의 경우는 100%와 18%였다. 가장 흔한 원인균은 methicillin-resistant S. aureus였다. Tc-99m MDP 스캔에서 양성소견을 보였던 환자중 3명의 환자가 Tc-99m HMPAO 백혈구 스캔에서 음성 소견을 보였으며 이 환자들은 추적관찰상 골수염이 없는 것으로 확인되었다. 결론 : Tc-99m HMPAO 백혈구 스캔은 골수염의 존재를 진단하는데 있어 민감도와 특이도가 높으며, 특히 뼈의 재형성이 증가되어 Tc-99m MDP 스캔의 특이도가 낮게 나타나는 상황에서 유용하리라 사료된다. Background Three phase bone scintigraphy, performed with technetium-labeled diphosphonates (Tc-99m MDP scan), is the very sensitive radionuclide procedure of choice for diagnosing osteomyelitis. But, Tc-99m MDP scan is less specific when bone remodeling is increased. To overcome this limitation, we evaluated the diagnostic value of technetium-99m hexamethylpropyleneamine oxime (HMPAO) labeled leukocyte scan in osteomyelitis. Methods : We retrospectively reviewed 15 patients with suspected osteomyelitis who performed Tc-99m HMPAO labeled leukocyte scan. The diagnosis of osteomyelitis was based on clinical course and bacterial culture, and pathologic findings. Results The sensitivity and specificity of Tc-99m HMPAO leukocyte scan was 100% and 78%, and those of Tc-99m MDP scan was 100% and 18%, respectively. The most common organism was Methicilein-Resistant S. aureus. Among the patients that showed persistent accumulation on Tc-99m MDP scan, three patients showed no uptake on Tc-99m HMPAO leukocyte scan and these patients were confirmed that had no osteomyelitis. Conclusion : Tc-99m HMPAO leukocyte scan is a useful test for determining the presence or absence of osteomyelitis, especially under condition that bone remodelin is increased.

학교 경제교육 방향 탐색을 위한 델파이 조사 연구

최병모,김정호,이준혁 한국경제교육학회 2005 경제교육연구 Vol.12 No.1

해수유동모델 결과의 3차원 가시화를 위한 GUI 구현

최우정,박성은,이원찬,구준호,서영상,김태현 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.3

해수유동모델 결과를 3차원적으로 가시화하기 위한 그래픽 유저 인터페이스를 구축하는 과정에서 지리정보시스템(GIS) 기술을 활용하였다. 연안역 관리에서 GIS 기법은 산재되어 있는 방대한 자료들을 저장하고 이를 3차원으로 가시화하는 기능을 제공하며 해석적, 통계적, 모델링 도구로써 주어진 상황에 적합한 데이터 변환이 가능하도록 해준다. 해수유동모델은 개방경계에서 조석, 바람, 수온, 염분, 강물 유입 및 태양복사 등과 같은 모델입력값을 부여함으로써 구동되며 대상해역은 진해만으로 하였다. 녈 연구에서는 국산 GIS 소프트웨어인 GeoMania (v2.5)를 사용하여 진해만을 대상으로 시뮬레이션된 해수유동모델 결과의 3차원 가시화 모듈을 구축하였다. This study presents an application of GIS technologies to construct the graphic user interface for 3-dimensional exhibition of the results obtained by ocean hydrodynamic model. In coastal management studies, GIS provide a receptacle for scattered data from diverse sources and an improvement of the 3D visualization of such data. Within the frame of a GIS a variety of analytical, statistical and modeling tools can be applied to transform data and make them suitable for a given application. A 3D hydrodynamic model was driven by time-dependent external forcing such as tide, wind velocity, temperature, salinity, river discharge, and solar radiation under the open boundary condition. The Jinhae bay was selected as a case study. Here, we have used GeoMania v2. 5 GIS software and its 3D Analyst extension module to visualize hydrodynamic model result that were simulated around the Jinhae bay.

2차원 도형의 블렌딩

최영,서준호 중앙대학교 생산공학연구소 1994 생산공학연구소 논문집 Vol.3 No.1

3차원 형상을 모델링하기 위해서는 CSG-tree, B-rep등의 데이터구조를 바탕으로하여, rounding, filleting, sweeping, boolean operation 등의 방법을 이용하여 형상을 모델링하게 되는데, 본 연구에서는 민코스키 연산을 이용하여 2차원상에서 곡선경계를 가지는 형상의 블랜딩을 구현해보고, 민코스키 연산을 이용하여, 일반적인 형상을 가지는 두 물체사이의 중간 형상을 자동으로 생성하게 하므로서, 민코스키 연산의 형상모델링 도구로서의 응용 가능성을 검토해 보았다. 민코스키 합을 구현하기위한 방법으로 convex decomposition을 이용했으며, 경계곡선을 근사화하는 과정에서 곡선을 생성하고 표현하기 위해서 biarc spline curve를 이용하였다. 두 형상에 가중치를 주어 두형상 사이의 점차적인 변화를 계산했다. Rounding, filleting, sweeping, and Boolean operations are the most popular tools for three dimensional modeling operation. In this paper the concept of Minkowski sum, that is vector sum of two sets, is adopted for the new tool in geometric modeling. Using the concept of Minkowski sum, shape blending operation of the two 2D polygons and curved boundary 2D shapes are implemented and demonstrated. Convex decomposition of arbitrary 2D polygon and curve approximation techniques are used in the process of the operation.

기저질환이 없는 사람에서 발생한 Campylobacter fetus에 의한 재발성 심내막염 1예

전재범,정진원,김성혜,송문희,오형철,안성기,최상호,김남중,김양수,우준희,류지소 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

C. fetus 심내막염은 드물게 발생하는 질환으로 주로 면역능이 저하된 환자에서 발병하고 대동맥판을 침범하는 경우가 많으며 적절한 치료가 되지 않을 경우 치사율이 높다. 저자들은 기저질환이 없던 69세 남자에서 C. fetus 심내막염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 발열로 내원하여 시행한 혈액배양 검사상 C. fetus가 동정되었고 경식도 초음파 검사상 삼첨판에 증식증이 발견되어 심내막염 진단하에 ceftriaxone에 이어 meropenem으로 총 4주간 항생제를 투여받고 호전되었다가 한달 후 다시 재발하였고 판막부전 소견 진행하여 삼첨판 치환술을 시행받고 전신상태 호전되어 퇴원하였다. Campylobacter fetus is a rare cause of endocarditis. To date, only about 30 cases of C. fetus endocarditis have been reported mainly in the elderly with chronic underlying illnesses. Most cases showed tendency to involve aortic valve. This report describes a case of native tricuspid valve infective endocarditis in a 69-year-old man without any underlying disease. Despite adequate antibiotic therapy for 4-weeks, endocarditis recurred and leading to surgical valve replacement.

<i>Toxoplasma gondii</i> protects against H₂O₂‐induced apoptosis in ARPE‐19 cells through the transcriptional regulation of apoptotic elements and downregulation of the p38 MAPK pathway

Choi, Si‐,Hwan,Park, Sung Jun,Cha, Guang‐,Ho,Quan, Juan Hua,Chang, Nam‐,Sik,Ahn, Myoung‐,Hee,Shin, Dae‐,Whan,Lee, Young‐,Ha Blackwell Publishing Ltd 2011 Acta ophthalmologica Vol.89 No.4

<P><B>Abstract.</B></P><P><B>Purpose: </B> Toxoplasmosis, which is caused by the protozoan parasite <I>Toxoplasma gondii</I>, can lead to severe visual impairment. <I>T.?gondii</I> inhibits or delays programmed cell death caused by various apoptotic triggers; however, the mechanisms involved in the <I>T</I>.?<I>gondii</I>‐induced suppression of apoptosis in retinal cells have not been analysed in detail.</P><P><B>Methods: </B> We investigated the role of <I>T</I>.<I>?gondii</I> infection in H<SUB>2</SUB>O<SUB>2</SUB>‐induced apoptosis in human retinal pigment epithelial cells (ARPE‐19) by monitoring the activities of apoptosis‐regulating molecules and mitogen‐activated protein kinases (MAPKs), including p38 MAPK. We also examined the gene downstream from p38 MAPK.</P><P><B>Results: </B> <I>T.?gondii</I> infection significantly inhibited the cellular toxicity of H<SUB>2</SUB>O<SUB>2</SUB> (500 μ<SMALL>m</SMALL>) and increased cell viability in a multiplicity of infection (MOI)‐dependent manner by reducing DNA fragmentation and reactive oxygen species (ROS) generation in ARPE‐19 cells. Western blot analysis also showed that <I>T</I>.?<I>gondii</I> infection prevented the host cell expression of pro‐apoptotic factors, such as Bad and Bax, and the activation of caspase‐3. Infection with <I>T</I>.?<I>gondii</I> increased the expression of the anti‐apoptotic factor Bcl‐2 in ARPE‐19 cells under oxidative stress. In accordance with these findings, <I>Toxoplasma</I> infection was protective enough to suppress the phosphorylation of p38 MAPK following H<SUB>2</SUB>O<SUB>2</SUB> treatment. Exposure to H<SUB>2</SUB>O<SUB>2</SUB> increased the expression of heme oxygenase‐1 (HO‐1) in ARPE‐19 cells, and its expression was significantly inhibited in H<SUB>2</SUB>O<SUB>2</SUB>‐treated infected cells.</P><P><B>Conclusion: </B> The protective function of <I>T</I>.?<I>gondii</I> infection against ROS‐induced apoptosis results from changes in the expression of apoptotic molecules and the downregulation of stress‐induced intracellular signalling.</P>

말기신부전 환자의 혈색소 순환 : 투석 방식에 따른 양상 비교 및 관련 인자들에 대한 고찰

최재혁,김준섭,원기범,박준형,탁우택,이정호 동국대학교 의학연구소 2009 東國醫學 Vol.15 No.2

혈색소 순환은 말기신부전 환자에서 인체 재조합 적혈구 조혈 인자를 투여하여 빈혈을 치료하는 동안 빈번히 관찰되며 이는 환자의 입원과 사망률과 관계가 있다고 알려져 있다. 이에 저자는 투석중인 말기 신부전 환자를 대상으로 혈색소 순환 양상과 이와 연관된 인자들을 알아보고자 하였다. 혈색소 순환의 관련 인자들을 알아보기 위하여 투석 치료를 받고 있는 환자 40명 (혈액투석 환자군: 20형, 복막투석 환자군: 20명)을 대상으로 투석 시작부터 1년 동안의 환자의 나이와 성별, 투석시작 1년간의 평균 Hb, 수혈 횟수, 총입원 횟수, 감염으로 인한 입원 횟수, 혈청 ferritin 농도, 혈청 transferrin saturation (TSAT), 철분제제 복용 유무, 일주일 동안 투여된 rHuEPO 양, rHuEPO의 변화량, 알부민, C-reactive protein (CRP) 및 입원 원인 등을 후향적으로 조사하였다. 전체 환자 40명 중 33명 (82.5%)에서 혈색소 순환이 관찰되었다. 혈액투석 환자군에 비해 복막투석 환자군에서 혈색소 순환 첫수가 유의하게 높았다 (p=0.037).혈색소 순환 발생군과 혈색소 순환 미발생군의 관련인자들을 비교하였을 경우 혈색소 순환 발생군에서 총 입원 첫수 (p=0.01), 감염으로 인한 입원 횟수 (p=0.036)가 유의하게 많았으며, 혈중 알부민 수치는 유의하게 낮았다 (p=0.003).그러나 두 군간의 나이, 성별, 수혈 횟수, 혈청 ferritin농도, 혈청 transferrin saturation (TSAT) 및 혈청 철분 농도는 유의한 차이가 없었다. 혈색소 순환의 빈도는 일주일 동안 투여된 r-HuEPO변화량과 양의 상관관계를 보였고 (p=0.003),혈중 알부민 수치와 음의 상관관계를 보였다 (p=0.013). 복막투석 환자군에서 혈액투석 환자군에 비해 혈색소 순환이 유의하게 높게 발생하였다. 일주일 동안 투여된 r-HuEPO 변화량, 입원 및 혈청 알부민 수치는 투석 시작부터 1년 동안의 혈색소 순환과 관련이 있었다. Hemoglobin cycling is frequently observed during treatment of anemia with recombinant human erythropoietin (r-HuEPO) in patients with end stage renal disease (ESRD) it is also reported to be associated with hospitalization and morbidity. We analyzed hemoglobin cycling and potential associated factors in patients on dialysis. Forty new dialysis patients (20 hemodialysis, HD and 20 peritoneal dialysis, PD) were recruited during their first year. Age, gender, mean hemoglobin level, frequency of transfusion, frequency of hospital admission, frequency of admission due to infection, serum ferritin, transferrine saturation, iron levels, rHuEPO dose per week, serum albumin, and c-reactive protein were retrospectively evaluated in order to examine the possible correlations with hemoglobin cycling. Thirty-three patients (82.5%) were identified in the hemoglobin cycling group. The PD group had a higher frequency of hemoglobin cycling than did the HD group (p=0.037). The hemoglobin cycling group showed a significantly higher frequency of hospital admission (P=0.001), admission due to infection (p=0.036) and a lower serum albumin level (p=0.003) than the other group, but there was no significant difference between the groups for age, gender, frequency of transfusion, serum ferritin, transferrine saturation, and iron levels. The frequency of hemoglobin cycling showed a positive correlation with the i-HuEPO dose variation per week (p=0.003) and a negative correlation with serum albumin level (p=0.013). The PD group had a higher frequency of hemoglobin cycling than the HD group, and the change of rHuEPO dose per week, hospitalization and serum albumin were associated with the hemoglobin cycling during the first year of dialysis.

육우 비선호 부위를 활용한 육우햄의 품질특성

이주호, 최정석, 정준영, 최양일 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.3

This study was undertaken to compare the quality characteristics of dairy beef ham. Dairy beef hams were manufactured using non-preferred portion (top round). 5 treatments were prepared: T1(beef 50% + pork emulsion 50%), T2(beef 65%emulsion 35%), T3(beef 80%emulsion 20%), T4(beef 90%emulsion 10%), T5(beef 100%emulsion 0%). In chemical composition, dairy beef content increases, moisture content was increased, fat content was decreased. In the meat quality characteristics, T5 showed higher (p<0.05) water holding capacity value than the others. Dairy beef content increases, product loss and cooking loss were decreased. In hunter color, dairy beef content increases, redness was increased. In texture profile analysis, T4 and T5 were significantly higher than the others. As a result, dairy beef ham with 80~90% dairy beef and 10~20% pork emulsion has low fat content, high product yield and superior texture profile, which could be developed as a dairy beef hams using non-preferred portion.

복합 치아종에 관한 증례보고

최형준,최병재,손흥규,양호정 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.2

The odontoma is relatively a common odontogenic tumor. It has come to mean as a growth in which both the epithelial and the mesenchymal cell exhibit complete differentiation, with the result that functional ameloblast and odontoblast form enamel and dentin. The etiology of it is unknown, but it has been suggested that local trauma or infection may lead to the production of such a lesion. Odontma is divided into 2 types. One is compound odontoma that is at least superficial anatomic simiarity to normal teeth, and the other is complex odontoma, that calcified dental tissues are simply an irregular mass bearing no morphologic similarity even to rudimentary teeth. Somtimes this malformation is not easily identified because its lacking of calcification. So, if permanant tooth is delayed to erupt, one should take a careful look the radiographic film to find that whether the cause of it is odontoma or not. In this case report, 3 patients with odontoma who visited Dept. of Pediatric Dentistry in Yonsei Dental College, were treated by means of surgical removal. The obtained results were as follows: 1. The odontoma is usually related to impacted tooth, and it is occurred neighboring to the crown of impacted tooth that have normally developed. So, in the case of missing tooth and delayed eruption, its presence should be investigated. 2. The odontoma can be seen vaguely radiographically, so, it is difficulty to make diagnosis. 3. The treatment of odontoma is a surgical removal. And, if it is completely removed, its occurrence is rare. 4. In the case of impacted tooth being associated with compound odontoma, continuous management is necessary for the eruption of impacted tooth after the surgical extraction of odontoma. 5. It resembles the ameloblastic odontoma radigraphically, and if it is the case, the treatment may vary, so the confirmation through histologic examination after removal of mass is necessary.