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      • KCI등재

        The Identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, Using Macroscopic, Microscopic and Physicochemical Methods

        Yoon, Jee-Hyun,Park, Ha-Yeong,Kim, Tae-Ju,Lee, Seung-Ho,Kim, Jung-Hoon,Lee, Guem-San,Kim, Hong-Jun,Ju, Young-Sung The Society of Korean Medicine 2014 대한한의학회지 Vol.35 No.4

        Objectives: This study aimed for establishing the discriminative criteria for Aucklandiae Radix, Inulae Radix, Vladimiriae Radix and Aristolochiae Radix, which can be confused as 'Mokhyang', because of similar appearances and synonyms. Methods: Morphological characteristics of dried herbal medicines were compared by macroscopic observation. To examine microscopic features of 'Mokhyang', paraffin embedding and the staining by using Ju's method were conducted. Physicochemical experiments were performed using HPLC analysis and antioxidant assay. Results: The types of stem, phyllotaxy and leaf shape were chosen as macroscopic criteria and the size, oil spots, color and root top as well as phloem and central cylinder were compared using microscopic features. The HPLC results showed different amounts of costunolide and dehydrocostuslactone among Aucklandiae Radix, Inulae Radix and Vladimiriae Radix. However, Aristolochiae Radix only contained aristolochic acid. The antioxidant assays also showed that Vladimiriae Radix exhibited strongest antioxidant activity followed by Aucklandiae Radix, Inulae Radix and Aristolochiae Radix. Conclusions: These results demonstrate that macroscopic, microscopic and physicochemical methods would be useful tools for the discrimination of 4 kinds of 'Mokhyang'.

      • KCI등재
      • 황정(黃精) 추출물의 화학구조결정에 관한 연구 (Ⅰ)

        윤중호,박주희,김정주,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

        본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 H 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E, 12E)-octadecadienoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoic acid.

      • KCI등재

        Changes in Block Exemption Applied to Maritime Transport and its Implication

        Yoon,Yu ri,Pak,Myong Sop,Hong,Ran ju 한국무역상무학회 2010 貿易商務硏究 Vol.48 No.-

        This study reviews maritime transport policy regarding liner conference and the changes in the liner market over the decades. Liner shipping industry has long been protected from competition by block exemption. To prevent excessive competition in punctual operation and its inelastic market structure, liner shipping companies formed conferences that are protected to fix the prices under the law. In the US, deregulation in transport sector began from 80's and continuing with OSRA 1998, conferences were dissolving. On the other hand, the EU with close conference system, Regulation 4056/86 contained block exemption remained in force for unlimited time without review clause. However, in Oct 2008, the EU has announced its removal, and conferences were no longer permitted to fix the price nor exchange information. Although OSRA 1998 has already broken up conferences by allowing individual service contracts, but the repeal of the immunity for price fixing will alter significantly the rule on cooperation in the industry since it is a unilateral move by the EU, especially in transatlantic lane. There are rapid changes in shipping market getting much more complicated, and with removal of 4056/86 allowing the market to be more competitive, opening up the industry with far more diverse strategic options. Hence this paper reviews on liner shipping industry and its changes of policies over the years from protected market to open competition market of today.

      • 鉛暴露 作業者의 鉛中毒 早期發見을 위한 集團檢診法 改善方案에 관한 硏究

        홍대용,김장락,정주화,최현림,윤형렬 慶尙大學校 1995 論文集 Vol.34 No.1

        This study was carried out to evaluate the feasibility of current lead indicators used in screening lead who were sspeted to have been exposed to lead were selected as 'case'and 207 female workers from non-lead industry were selected for 'control'. The results obtained were as follows: 1. The mean values of blood lead for case and control were 31.4㎍/dl, 22.2㎍/dl,respectively. The difference observed in the mean concentration between case and control was statistically signifi-ant(P<0.01) 2. The mean values for blood ZPP by hematofluorometer for case and control were 31.3㎍/dl, 25.4㎍/dl,respectiely. And difference between two groups was statistically significant(P<0.01). 3. No statistically significant correlation was found between working duation and blood lead, blood ZPPlovel. 4. According to the simple correlation analysis,ZPPand urine lead were found to be statistically correlated with blood lead(P<0.01) 5. According to the discriminant analysis using blood lead as a dependent variable, it showed 89.1% predictability when using 40㎍/dl as a cutting value which is used as a screenig cutting value for industry workers health examination, while 81.8% for 30㎍/dl,77.54% for 20㎍/dl, respectively. 6. Lead related simptons which is used for screening the lead ntoxicated patients were used as a dependent variables in multiple regression analysis to find useful lead indicator. And significant independent variables were found to be ZPP, Age, and Hct. 7. Above results suggest that objective evalution of feasibbility of several lead indicators should be developed to correct the disutility of current lead screeng system in Korea.

      • 흡입물질의 위생학적 연구 : 본드 흡입이 흰쥐의 폐 및 기타 장기의 효소 활성에 미치는 영향 The Effect of Bond Inhalation on the Enzyme Activity of Lung and Other Organs in Rat

        윤수홍,박은주,이주영,서혜령 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

        Toluene, alcohol및 vinylester류 등을 함유하고 있는 bond를 이용하여 휘발성 물질이 생체에미치는 영향을 관찰하기 위하여 rat에 일정 농도의 bond를 일정 시간 흡입시킨 뒤 각 조직에대하여 효소의 생화학적 시험과 celluloseacetate electrophoresis를 실시하여 다음과 같은 결과를얻었다. 1. Bond 흡입은 시간이 증가함에 따라 혈청, 심장, 신장, 근육, 뇌의 ChE 활성을 억제하였으며특히 폐와 간장의 ChE활성에 가장 큰 영향을 나타내었다. 2. Bond 흡입에 의해 혈청 및 각 조직 중에서 LDH활성이 증가되었으며 특히 폐와 심장,근육에서 그 영향이 현저히 나타났다. 3. Celluloseacetate electrophoresis를 통한 각 조직의 LDH isozyme pattern에는 차이가 나타났으며 bond 흡입에 의해 band의 소멸과 선명도의 감소가 일어 났는데 특히 폐에 대한 독성이가장 현저했으며 근육에 대한 독성도 큰 것으로 나타났다. Acute poisoning with organic solvents and other volatile compou-nds now usually follows deliberate inhalation(volatile substance abuse) or inges-tion of these compounds. The effect of bond inhalation was analyzed for serum, liver, lung, brain,heart, kidney and muscle. And the observations are revealed on rat cholinesterase activity, lactatedeh-ydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows ; 1. The rat cholinesterase activity on serum, heart, muscle, kidney and brain showthe decrease by increasing of inhalation time of bond, in particular the lungand liver cholinesterase activity was greatly affected. 2. Bond inhalation brough out the lactatedehydrogenase activity increased ofthe serum and the tissues and had an important effect especially in both thelung and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydro-genase isozymes on celluloseacetate elelctrophoresis and the development ofinhalation time is shown the disappearence and diffusion of band.

      • 천연자원의 간기능 보호 효과에 관한 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 강활의 효과 The Effect of Angelica koreana on Benzo(a)pyrene Induced Hepatotoxicity

        윤수홍,이송애,박은주,이주영 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

        민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화물의 불완전 연소 및열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성 발현에미치는 강활 수침액의 예방 및 치료 효과를 실험하여 다음과 같은 결과를 얻었다. 1. 강활 수침액의 투여는 B(a)P투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH,ALP 활성의 유의성 있게 감소시켰다. 2. B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량은 강활 수침액의 투여로감소시킬 수 있었다. 3. 강활의 투여는B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of crude Angelica koreana reported with the clinical uses in the orientalmedicine. Crude angelica. used for the treatment of common cold, headache, neu-ralgia and arthralgia. Therefore we tested the effects of Angelica water extract on the liver-protec-live activities in the rats. The results obtained from liver microsomal enzymeassay, measurement of serum and liver alanine' aspartate aminotransferase andlipid accumulation indicated that angelica extract showed significant liver-protec-tive activities against benzo(a)pyrene poisoning.

      • Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 세신 추출액의 영향

        정영건,권오진,윤수홍,이송애,이주영 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

        탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 다환방향족 탄화수소로 간 실질세포에서 cytochrome P_450에 의해 산화되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의간독성 발현에 미치는 세신 수침액의 예방 및 치료 효과를 실험한 결과 세신 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 transaminase 활성을 유의성 있게 감소시켰으며 그효과는 조직에서 보다 우세하였다. 그러나 세신 수침액이 B(a)P투여로 증가한혈청 total cholesterol과 phospholipid 함량을 감소시킬 수는 있었지만 그 효과는 약하여 지질대사에는 세신의 작용이 미미할 것으로 보인다. Effects of Asiasari Radix, which is herbal drug used frequentlyin the oriental prescriptions, water extract on the liver-protective activities wereinvestigated in the rats. Asiasari Radix water extract, when administered intothe gastric intubation, produced liver-protective effect against benzo(a)pyreneinduced liver damage. The results obtained from enzyme assay, measurement of serum and liveralanine aspartate aminotransferase(AST, ALT) and lipid composition indicatedthat Asiasari water extract showed significant liver-protective activities againstbenzo(a)pyrene-induce hepatotoxicity.

      • 간세포암종에 대한 간동맥 화학색전술 후 발생한 급성호흡곤란증후군

        조윤원,이정미,최자윤,유동훈,차라리,오혜원,김홍준,민현주,김현진,정운태,이옥재,하창윤,이선영 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.1

        Transcatheter arterial chemoembolization (TACE) has become an effective alternative treatment strategy for patients with inoperable hepatocellular carcinoma (HCC). Although TACE is relatively safe, acute respiratory distress syndrome associated with pulmonary lipiodol embolism is a rare and potentially fatal complication. We report a rare case of acute respiratory distress syndrome after TACE for inoperable HCC. A 75-year-old man, with huge HCC in right lobe, was treated by TACE for the first time. Seven hours after uneventful TACE procedure, he felt dyspneic and his oxygen saturation recorded by pulse oximetry (SpO2) fell to 80% despite of applying non-rebreathing mask. He underwent mechanical ventilation with a protective ventilatory strategy. We experienced a case of acute respiratory distress syndrome after TACE for HCC.

      • KCI등재

        새터민의 기초직업능력 진단

        이찬,이용환,이윤조,신재호,홍윤선,최홍주 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.3

        이 연구는 새터민의 고용현황 및 문제점을 고찰하고 새터민의 기초직업능력을 진단하여 직업능력개발 방향에 있어 시사점을 제공하는데 그 목적이 있었다. 새터민의 기초직업능력 진단을 위한 도구는 관련 문헌 고찰과 pilot test 및 새터민 교육ㆍ지원 담당자의 안면타당도 검증 등을 통하여 개발되었다. 연구의 목적을 달성하기 위하여 현재 하나원에서 사회 적응교육 및 직업교육을 받고 있는 새터민의 기초직업능력을 진단함으로써 우리나라에 거주하는 새터민의 기초직업능력을 파악하였다. 주요 결과는 다음과 같다. 첫째,새터민들의 기초직업능력 중에서 직업윤리능력은 상당히 갖추어져 있는 것으로 인식하고 있으며,정보능력,즉 컴퓨터 사용능력은 상대적으로 낮은 수준으로 평가하고 있다. 둘째,성별에 따라서는 기초직업능력에 있어서 통계적으로 유의미한 차이를 보이지 않았다. 연령에 따라서는 정보능력과 수리 능력,조직이해능력 영역에 있어서 통계적으로 유의미한 차이를 보였다. 셋째,학력에 따라서는 문제해결능력과 의사소통능력,수리능력,대인관계 영역에 있어서 통계적으로 유의미한 차이를 보였다. 넷째,새터민의 취업을 위한 기초직업능력 개발에 있어 정보능력,수리능력,의사소통능력의 비중 확대,수준별ㆍ맞춤형 교육프로그램개발,새터민을 위한 취업 및 사업정보 제공 등을 제시하였다. The purpose of this study was to explore the current employment status and HRD issues of North Korean refugees and to assess their level of the basic vocational competencies. The assessment tool for the basic vocational competencies was developed based on literature reviews and pilot tests from the North Korean refugees. The face validity was verified by educators and counselors for the North Korean refugees. The major findings in this study were as follows: First, among the basic vocational competencies of the North Korean refugees, the level of vocational ethics was the highest and the level of information teachnology competency was the lowest. Second, the North Korean refugees' basic vocational competencies showed no statistical differences by gender. Territories of information competency, mathematical competency, and communication competency showed statistical differences by age. Third, territories of problem-solving competency, communication competency, mathematical competency, mathematical competency, and communication competency showed statistical differences by education level. Fourth, to develop the basic vocational competencies for North Korean refugees, the following suggestions were presented.; 1) extend the importance of information technology, mathematical, and communication competencies,; 2) develop the customized training programs by competency level,; and 3) offer the employment information for North Korean refugees.

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