마에 의한 비염,구강 알레르기 증후군이 동반된 천식 증례

석수진,박종숙,김덕수,손범석,박춘식 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2S

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알지네이트 비드를 이용한 메틸 오렌지의 봉입 및 방출에 미치는 키토산의 영향

변종찬, 이상은. 장우석, 이은주, 최진석, 박정숙 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-

In this study, methyl orange-loaded alginate beads were prepared and compared to investigate the effect of chitosan on the encapsulation and release of hydrophilic methyl orange in alginate beads. Particle size and encapsulation efficiency of alginated beads were determined. Swelling ratio and disintegration of al-ginate beads were investigated in gastrointestinal environment. Particle size of beads was 1.1-1.5 mm and shape was spherical. Weight of beads was increased depending on the content of chitosan. Content of chitosan affected on the encapsulation of methyl orange. Encapsulation of methyl orange in alginate beads without chitosan was 15%, while encapsulation efficiency was increased with addition of chitosan. Beads were not disintegrated in HCl solution (pH 1.2) within 4 h, with floating and swelling. Swelling ratio of beads was in-creased to 222%. Release of methyl orange from alginate beads without chitosan was up to 70% within 2 h in HCl solution (pH 1.2). When chitosan was added to the alginate beads, release of methyl range from beads was significantly reduced. It is supposed that release of methyl orange from beads is resulted from diffusion and erosion of beads, which is pH-dependent. In condusion, chitosan-alginate beads have potentials to control release of small hydrophilic drugs by the addition of chitosan.

대전대학교내 먹는 지하수의 미생물학적 수질 평가

방숙전,안종훈,박성주 대전대학교 기초과학연구소 1997 自然科學 Vol.8 No.1

지하수 원수의 세균수는 수온과는 상관성이 없었으며, 종속영양세균의 경우 수온이 가장 낮았을 때 개체수가 가장 많았다. 원수의 평균 총세균수와 R2A배지에서 자란 종속영양세균수는 각각 6.9x10? cell/㎖, 6.7x10²CFU/㎖ 이었으며, 수도꼭지의 물에서도 세균수의 변화는 별로 없었다. R2A배지에서 종속영양세균수는 PCA배지에서 자란 종속영양세균수에 비하여 대체로 10배 정도 많았는데, 이는 유기물의 농도가 낮고 세균수가 적은 지하수 세균학의 연구에 R2A배지의 사용이 효과적이라는 것을 보여주는 것이다. 지하수 원수에 비하여 수도꼭지의 물에서 세균수가 현저하게 증가된 이른바 급수관에서 재생장 현상을 보인 세균은 종대장균군으로서 원수에서 8 CFU/250㎖로 35배나 증가하였다. 분변성 연쇄상구균도 급수계통에서 심한 재생장 현상이 나타났으며 분변성 대장균둔이 검출되지 않은 물에서도 검출되는 것으로 보아 지하수의 지표세균우로 필요한 것으로 판단된다. Salmonella와 Shigella는 추정시험용 선택배지에서 정형적인 콜로니가 나타나기는 하였지만 동정결과 진성인 것은 하나도 없었다. Bacterial numbers in raw groundwater were not independent of water temperature, rather the highest number of heterotrophic plate count bacteria (HPC) was recorded at the lowest temperature. MEAN numbers of total and HPC bacteria in raw water were 6.9x10? cell/㎖ and 6.7x10²CFU/㎖ respectively, not changnin even in tap water. HPC numbers enumerated by R2A agar media were generally 1 log higher than by plate count agar (PCA) media, showing R2A is preferable to PCA in the study of groundwater bacteriology having low organic matters and bacterial density. Total coliform (TC) densities tremendously increased in tap waters and coliform regrowth in the distribution system occurred. Eight CFU/㎖ of total coliforms in raw water changed to 281 CFU/250㎖ in tap water, 35 times increase in density. Presumptive fecal streptococci (FS) showed regrowth in the distribution system as well, but presumptive fecal coliforms did not. HIgh FS numbers were recorded even in the samples where no or few TC were detected, which represents FS as well as TC are necessary to assess drinking groundwater quality. Even though some typical colonies of Salmonella or shigella were developed on the presumptive selective media, any true positive pathogens were not identified.

결식아동의 실태와 특성

박장숙,전진호,김성준,손혜숙,이종태 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : The unprivileged children without lunch(UCWL) has been being markedly increased and becoming one of the social problem in Korea. This study was performed to assist the development of efficient support program through the investigation of the status and characteristics of the UCWL. Methods and Material : Subjects were 104 students sho were receiving the meal aid from the six social welfare facilities and 110 ordinary students of an elementary school in Pusan area. Questionnaire survey was conducted from February to March, 2001. Data was analyzed using SPSS program(ver 10.0). Results : There was no difference in gender and school years' distribution. The phenomenon of family deficient, instability of parents' job, unsatisfaction to the home's atmosphere, ill adaptation to their school life, and be alienated from their colleague were more frequent in UCWL(p<0.05). Impulse of runaway from home and suicide showed no difference, however, the present worries, preference of way for assist showed differences(p<0.05), The majority(88%) of UCWL has been giving the meal and less than on year. The most common way was food stuff(76%) and financial support for school meal program(73%), while the most preferred way was financial support for everyday life(43%). Conclusion : UCWL satisfied and gave thankful mind to the present supports, however, showed negative aspects - like emotional unstability and difficulties in their school life. Therefore, it thought to be needed the touch on the emotional aspects, not only on the formal and economic aids.

갑상선 침세포진 시료의 주사전자현미경적 관찰

박성진,이숙희,조창호,채종민 慶北大學校 醫科大學 1995 慶北醫大誌 Vol.36 No.3

목적 : 갑상선 결절에 대한 수술전 진단을 위하여 침세포진 검사는 매우 유효하며 빈번히 시행되고 있다. 그러나 집단으로 도말된 세포들에서 때때로 종양세포의 감별진단이 어려운 경우가 있다. 주사전자현미경에 의한 세포들의 배열이나 세포 표면에 대한 관찰은 매우 드물다. 본 연구는 이러한 침세포진 검사에 있어서 주사전자현미경적 관찰을 감별 진단에 이용하고자 한다. 대상 및 방법 : 1994년 5월부터 1995년 4월까지 경북대학교병원에서 갑상선 침세포진 검사를 하고 갑상선 절제술을 시행하여 확진된 56례의 시료를 주사전자현미경으로 관찰하였다. 결과 : 결절성 비대증을 포함한 양성 병변에서는 상피세포 표면에 미세 응모가 잘 발달되어 있었으나 악성 종양의 상피세포 표면에는 미세 융모의 감소 및 완전 소실이 관찰되었다. 그러나 유두상암종에서는 미융모가 발달되어 있었으나 매우 불규칙하였다. 그리고 광학현미경상 혼돈이 되는 소포성 및 유두상 구조물의 감별이 용이하였다. 결론 : 이상의 성적으로 보아 갑상선 결절성 병변의 침세포진 검사에 있어서 감별 진단이 어려운 경우 이를 주사전자현미경으로 관찰하는 것이 감별진단에 도움이 될 것이라고 생각된다. Fine needle aspiration cytology is widely accepted as a primary method for the diagnosis of thyroid lesions. To ascertain the effective value of scanning electron microscopic features of fine needle aspiration cytologic specimens, the scanning electron microscopic study of 56 various thyroid lesions was carried out. Well developed microvilli were observed in the follicular surface almost every epithelial cell in the nodular hyperplasia. Their number was reduced in adenomas and differentiated carcinomas. Medullary carcinomas and anaplastic carcinomas usually lack microvilli. Papillary carcinomas on the other hand have abundant microvilli, but their shape was much irregular. Follicular and papillary structures were easily identified by scanning electron microscopic observation. The results suggested that observing by scanning electron microscopy of fine needle aspiration cytologic specimens may be helpful in the differential diagnosis of thyroid lesions.

산업 근로자의 구강건강실태 및 구강질환 관련요인에 관한 연구

김혜진,박천만,우극현,이은숙,이종렬,하은경 대한보건협회 2002 대한보건연구 Vol.28 No.2

To maintain and improve workers' oral health, I investigated and analyzed the oral health status of workers, who are working at companies located in Koomi City. The results of the investigation and analysis are as follows. 1. Regarding the general properties of investigated objects, the male ratio of whole objects(1299 persons) occupied 86.6% the larger percentage, the persons from 20ages to 29ages in terms of age distribution occupied 35.6%, and the high school graduators in the respect of education occupied 75.3%. As for the property of duties, technical workers occupied 83.6% the larger percentage. In the drinking frequency of habits, one or two times drinking per week occupied 34.1% in case of male, and no drinking occupied 62.9% in case of female. 2. In the existence and nonexistence of oral disease depends on the general property of each objects, many females have an oral disease in comparison with males(p<.05), many persons with low education level have an oral disease(p<.001), and lots of self-boarding persons have an oral disease compared to the persons stayed at own home or dormitory(p<.05). In the existence and nonexistence of oral disease depends on living habits, the more male has a drinking capacity, the more has an oral disease(p<.05). And, the more male is smoking, the more has an oral disease(p<.05). When examine oral disease in accordance with cubjective healthy condition, there were many respondents answered that I have a decayed tooth or periodontitis in comparison with respondents did that I' m healthy(p<.001). In the existence and nonexistence of oral disease depends on oral health control practices and recognition, the persons received scaling for the latest one year had show a few of oral diseases significantly(p<.001). 3. As a result of Logistics Regression Analysis by putting oral disease as a dependent variable, the person who does not receive scaling, does smoking, has a low education level, does work at company for a short time, experiences lots of subjective symptoms, and does drinking, has a possibility that he/she can be infected easily with oral disease. As a whole of the above results, regular oral checkup every year shall be strengthen in its quality and quantity. In particular, the activation of oral health education to prevent oral disease may make workers' oral health and their life quality to be improved. Accordingly, in oral health education for workers, the more systematic and effective program shall be developed and the supports in government policy shall be needed.

梧桐島 植物相에 關한 硏究(Ⅱ) : 草本分布調査 및 生態學的 考察 Distritution and Ecological study of Herbs

張錫模,金琮鴻,林行鎭 順天大學校 1983 論文集 Vol.2 No.1

1982年 4月부터 1983年 8月까지 梧桐島의 植物相을 調査한 結果 草本은 總 56 科 156屬, 176種 26變種 1品種으로 Plant form은 Perennial herbs가 81種으로 가장 많고 evergreen herbs도 8種이나 調査 되었으나 大部分 路傍植物들이었다. 地被植物로서 常綠性草本은 Sphenomeris chusana, Cheileanthes chusana, Cyrtomium falcatum Cyrtomium caryotideum var. koreanum, Rumohra amabilis, Lastrea glanduligera, Asplenium sarellii等이고 Liriope spicata와 Liriope platyphylla도 常綠으로서 越冬하고 있었다. Climbing herbs인 Dioscorea batatas, Diascorea tokolo, Dioscorea nipponica, Humulus japonicus, Pleuropterus multiforus, Persicaria perfoliata, Cayratia japonica, Trichosanthes kirilowii等이 常綠闊葉樹의 林冠을 덮어 상당한 被害를 주고 있었다. 本島의 自然植生의 毁損과 破壞는 人間千涉에 依한 것이므로 觀光客의 覺醒이 촉구될 뿐이다. Investigating the aspects of the vegetation of Ohdong-Do island from April, 1982 to August, 1983, the author found out that the kinds of the plants growing in the island amounts to 56 families, 156 genuses, 26 varieties, and one breed and that as for plant form, perennial herbs, 81 species, were of a largest number and evergreen herbs, 8 species discovered, were for the greater part roadside plants. There discovered cover evergreen herbs like Sphenomeis, chusana, Cheileantes, chusand, Cyrtomium falactum, Cyrtomium carytideumvar. koreanum, Rumohra amabilis, Lastrea glanduligera, Asplenium sarellii ect., and also found out wintering evergreen herbs like Liriope spicaia and Liriope platyphylla. Dioscorea batatas, Dioscorea tokolo, Dioscorea nipponica, Humulus japonicus, Peuropterus multiflorus, Persicaria perfoliata, Cayratia japonica and Trichosanthes kirilouii etc., which are in climbing herbs, were covering the canopies of broad leaves trees and causing damages to them. Since the damages and destructions of the natural vegetation of the island were brout about from human intervention, the spiritual awakening of tourists are vitally needed.

Catecholamine 길항제 및 morphine 길항제 투여시 GABA 동작성약물이 진통효과에 미치는 영향에 관한 연구

함진숙,송형근,김중수,이종흔 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.2

This experiment was performed to study the GABAergic function in descending analgesic mechanisms from periaqueductal gray matter and to clarify the relationship of GABA to noradrenaline or opioids. Sprague-Dawley rat was anesthetized and cannula was inserted into femoral vein for injection of yohimbine, catecholamine antagonist and naloxone, opioid antagonist. A pair of stimulating electrodes were inserted into palatal mucosa and anterior belly of digastric muscle was exposed and a pair of electrodes were inserted into muscle to assess the EMG of digastric muscle. The threshold intensity of the noxious stimulus which induced the minimal dEMG activity was established and twice as much stimulus as the threshold value was applied to oral mucosa. PAG was stimulated with 200μsec duration, 200Hz frequency for 200msec and jaw opening reflex was evoked by stimulation of palatal mucosa with 30msec delay after offset of PAG stimulation. The inhibitory effects of PAG stimulation on dEMG were evaluated. Muscimol (62.5ng) was infused in 5μl saline by ICV method. Naloxone (0.8mg/kg) or yohimbine (2mg/kg) was administered 15min after completion of muscimol injection by IV method. The dEMG was recorded at 15min interval for 2 hrs. The dEMG was decreased after muscimol was injected, and the dEMG of PAG-stimulated rat was increased to the control value after naloxone or yohimbine only was injected. After muscimol injection, injection of yohimbine or naloxone antagonized synergistic effect of muscimol on PAG stimulated effect.

우리나라 지역 직업성질환 감시체계의 현황과 전망

임종한,장성실,김성아,문재동,채창호,홍윤철,김수영,김진석,김영욱,한상환,이혜숙,원종욱,송동빈,하은희,강성규 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

기존의 특수건강진단과 작업환경측정을 통한 직업병 관리가 진폐증, 소음성난청 등의 소수 특정질환에 국한되고 실제 직업병 발생 규모 파악이나 신종 직업병의 발견에 한계를 보인다는 사실은 산업의학전문가들 사이에서도 공감을 이루고 있다. 미국과 영국 등에서의 직업성질환 감시체계에 대한 경험은 우리 나라의 직업성질환 감시체계 구축에도 새로운 자극제가 되면서, 1998년이후 인천, 대전, 여천, 구미, 부울경 지역에서 지역 직업성질환감시체계를 산업보건관리의 중요한 시스템으로 구축하려는 노력이 확산되고 있다. 새로이 구축되어지는 이들 지역 직업성질환 감시체계는 감시하고자 하는 대상질환, 활용 가능한 인적자원 및 자료원, 지역 의료체계의 특수성 등에 따라서 목적과 방법을 달리하면서 독특한 형태로 발전을 하고 있다. 각 지역단위 감시체계들이 그 상황에 맞게 독특한 목적과 전략들을 발전 시키면서도, 향후 발전할 국가적인 차원의 직업성질환 감시체계 구축을 위하여 직업성질환 감시의 기본 전략 등을 공유하는 등의 노력이 필요하다. 환례 정의 및 기본적인 등록 서식의 공유, 직업성질환 감시 자료원의 발굴, 공동의 정보 네트워크 및 직업성질환 감시 데이터베이스 구축 등 직업성질환 감시활동을 지원하기 위한 여러 기초 인프라 구축에 힘을 모아야 할 것이다. 우리 나라에서 직업성질환 감시체계를 성공적으로 구축하기 위해서는 수집된 자료의 질 관리를 위한 직업성질환 감시의 원칙 제정과 감시 전략의 공유 등이 필요하며, 전국적인 직업성질환 감시체계의 하부구조라고 할 수 있는 지역감시체계의 기초 토대 마련과 강화작업이 절실하게 필요하다.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.