한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

다중 중합효소 연쇄반응을 이용한 소의 Johne병 진단 기법 확립

김종배,송혜원,김근희,김홍,신광순,김두 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

반추수에서 발생하는 Johne병의 조기 진단 방법을 제시하고 이 질병의 원인체와 미생물학적 특징이 유사한 M. bovis, M. avium 등의 mycobacteria감염증을 감별 진단하는 방법을 개발하기 위하여 Mycobacterium 균속의 표준균주를 사용하여 중합효소 연쇄반응을 확립하였다. Johne병으로 의심되는 소의 혈액과 유즙을 채취하여 분리한 단핵구 및 거식세포로부터 genomic DNA를 추출하였다. 각 시료로부터 추출한 DNA를 template로 이용하여 Mycobacterium spp.에 특이적인 16S rDNA primer set를 이용한 PCR을 수행하여 시료내의 mycobacterial DNA 보유 여부를 확인하였다. 한편 mycobacteria 양성으로 확인된 시료는 M. avium complex 균종에 특이한 16S rDNA 염기서열을 기초로하여 제작한 primer set와 M. paratuberculosis 의 IS900 sequence에 특이한 primer set를 이용하여 duplex PCR을 수행하여 Johne병 원인체의 보균 여부를 조사하였으며, 이 결과를 oligonucleotide probe를 사용한 Southern blot hybridization을 통하여 다시 확인하였다. 이와 같은 duplex PCR 기법을 실제 축산 현장에서 수집한 유즙과 말초혈액으로부터 분리한 단핵구 및 거식세포 시료에 적용한 결과 본 연구에서 확립한 duplex PCR기법 유용성을 확인할 수 있었다. Diagnosis of Bovine Johne's Disease Using Multiplex Polymerase Chain Reactions In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and IS900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

Correction: Bioinspired M-13 bacteriophage-based photonic nose for differential cell recognition

Moon, Jong-Sik,Kim, Won-Geun,Shin, Dong-Myeong,Lee, So-Young,Kim, Chuntae,Lee, Yujin,Han, Jiye,Kim, Kyujung,Yoo, So Young,Oh, Jin-Woo Royal Society of Chemistry 2017 Chemical Science Vol.8 No.2

<P>Correction for ‘Bioinspired M-13 bacteriophage-based photonic nose for differential cell recognition’ by Jong-Sik Moon <I>et al.</I>, <I>Chem. Sci.</I>, 2017, DOI: 10.1039/c6sc02021f.</P>

정신분열병과 N-Methyl-D-Aspartate 수용체 유전자와 연관된 VNTR 표지자인 D9S158 다형성의 연합

김종원,채영규,정은기,김길숙,박강규,이영호,최인근,양병환,한진희 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

정신분열병과 NMDA 수용체와 연관된 D9S158 유전적 표지자와의 연관성을 알아보기 위해 75명의 정신분열병 환자군과 87명의 정상대조군을 대상으로 중합효소연쇄반응을 이용하여 연합연구를 시행하여 다음과 같은 결론을 얻었다. D9S158 유전좌위에는 모두 8개의 대립유전자가 있었고, 각 대립유전자는 cytosine-adenine염기가 반복되는 217염기쌍에서 233염기쌍의 형태였다. 먼저 정신분열병 환자와 정상대조군의 대립유전자의 빈도를 비교해 본 결과 통계적으로 유의한 차이를 보이지 않았다(p〉.05). 환자군을 좀 더 동질적으로 만들기 위해 정신분열병의 가족력 유무, 음성 및 양성증상, 연성신경학적 징후, DSM-IV 임상아형에 따라 환자군을 나누어 대립유전자의 빈도를 비교해 본 결과, 특정 임상양상과 관련이 있는 대립유전자를 발견하지 못하였다. 결론적으로 본 연구에서는 D9S158 유전좌위와 정신분열병의 연관성을 입증하지 못하였으나 NMDA 수용체 유전자주위의 다른 표지자나 좀 더 유전적으로 동질한 환자군을 대상으로 한 추후연구가 필요할 것으로 생각된다. An association study with Korean schizophrenic patients(N=75) and normal controls(N=87) was performed to find the relationship between D9S158 polymorphism and schizophrenia using polymerase chain reaction. Eight different alleles of a dinucleotide polymorphism on D9S158 locus were observed in both group. When we compared the frequencies of alleles between schizophrenics and normal controls, there was no significant difference between two groups. To increase homogeneity of schizophrenic group, we divided schizophrenic group by clinical phenotypes such as family history, negative and positive symptoms(PANSS), soft neurologic signs(NES-K) and DSM-IV diagnostic subtypes. Then we compared the frequencies of alleles among subgroups of clinical phenotypes, and there were no significant differences between subgroups(p〉.05). Although our findings fail to provide an evidence of association between schizophrenia and D9S158 locus, further investigation of other loci that are linked to NMDA receptor gene may be needed in genetically homogeneous subgroups of schizophrenia.

토양에서 분리한 Toluene 분해 세균의 동정 및 일부 특성

김종구,박근태,이건,이상준,손홍주 부산대학교 환경문제연구소 1998 環境硏究報 Vol.16 No.-

경상남도 양산천 일대의 토양시료로 부터 toluene을 유일한 탄소원 및 에너지원으로 생육하는 미생물들을 분리하였고, 그 중 toluene의 분해능이 가장 우수한 균주를 선별하여 공시균으로 선택하였다. 공시균의 형태학적, 배양적, 생화학적 및 화학분류학적 제반 특성들을 토대로 분류학적 위치를 검토한 결과 Rhodococcus 속으로 동정되어 편의상 Rhodococcus sp. EL-43P로 명명하였다. Rhodococcus sp. EL-43P는 phenol 및 다양한 phenol 유도체, benzene 등의 방향족화합물 기질에서 생육할 수 있었다. 특히 phenol 분해에 관련된 각종 환경조건을 최적화시키면 실제의 phenol 함유 폐수처리공정에서 충분히 적용가능성이 있음을 알 수 있었다. Rhodococcus sp. EL-43P는 catechol 1,2-dioxygenase의 활성을 가지고 있어 ortho pathway에 의하여 phenol을 분해함을 알 수 있었다. 또한 본 공시균은 toluene dioxygenase의 활성도 가지고 있었다. The microorganisms which degrade toluene as the sole and energy source were isolated from soil around Yangsan river. Among them, the strain EL-43P had the highest toluene degradability and identified as a Rhodococcus sp. according to morphological, cultural, biochemical and chemotaxonomical properties. Rhodococcus sp. EL-43P was able to grow at the various chlorinated phenols such as o-, p-chlorophenol, 2,3-,2,5-, 2,6- and 3,4-dichlorophenol, and benzene, respectively. If detailed phenol degradation conditions are established, the treatment of phenol wastewater using Rhodococcus sp. EL-43P would be possible. Rhodococcus sp. EL-43P had the catechol 1,2-dioxygenase which degrades Phenol derivatives by ortho pathway. This strain also had toluene dioxygenase.

PWR에 使用되는 우라늄의 精製 및 濃縮에 關한 硏究(Ⅰ) : 國內産 우라늄 鑛石에서 黃酸浸出과 溶媒抽出에 依한 우라늄精製 Uranium concentration from the Domestic Uranium ores by Sulphuric Leaching and Solvent Extraction

金鍾一,朴永錫,丁梧鎭,李秀一,金熙峰,裵相龍,秋相根 조선대학교 동력자원연구소 1986 動力資源硏究所誌 Vol.8 No.2

This research was undertaken to study the mineralogy, and the optimum conditions for uranium leaching and extraction from the Okchon system graphite ores in the Kumsan areas. A research has been needed to study the refining process for the effective leaching and extraction from uranium ores in the Kumsan area which has more uranium deposits than any other area. By the mineralogical study with the powder X-ray diffraction method, primary uranium minerals are uraninite, brannerite, coffinite etc. and secondary uranium minerals are uranocircite, niobium uranium oxide, carnotite, toberite etc. has developed in the studied area uranium ores. The volumetric ratio of the primary and the secondary uranium minerals are approximatly 1 : 5. In this experiment, the uranium-bearing graphite in the Kumsan area was milled with a jaw crusher and ballmill. This milled uranium-bearing graphite was leached by sulfuric acid and from this sulfuric acid-leaching solution, uranium was extracted by Amberlite LA-1 in kerosene. The results are as follows: (1) The optimum results in the leaching process are : acidity pH = 1.0, temperature : 25.0℃, particle size : -80+120 mesh, leaching time : 24 hours, pulp density : 50% solid (2) A good pH range for uranium extraction was between 0.8 and 1.3. (3) The uranium distribution coefficient increases in direct proportional to the concentration of the Amberlite LA-1 as extractive agent. (4) The optimum time at 200rpm was 15 minutes.

[논문]억지말뚝을 이용한 자립식 흙막이 공법의 해석기법 개발

권정근,임종철,김창영 釜山大學校生産技術硏究所 2008 生産技術硏究所論文集 Vol.67 No.-

억지말뚝을 이용한 자립식 흙막이 공법은 많은 실험과 현장적용을 통해 경제적이며 효율적인 공법으로 입증되었다(김,2006). 그러나 지반공학적인 이론적 확립이 되어 있지 않았다. 이에 이론적인 해석기법의 개발을 위해 실제 시공여건을 반영한 단계굴착을 통한 실내모형실험이 수행되었다. 본 연구에서는 단계굴착에 따른 흙막이 벽체 및 억지말뚝의 거동과,단계굴착에 따른 흙막이 벽체와 억지말뚝에 작용하는 토압과 응력을 분석하였다. 그 결과를 바탕으로 지반의 파괴 메카니즘을 제안하였으며 연결부,엄지말뚝,억지말뚝의 해석기법을 개발하였다.

Streptococcus mutans의 치태형성에 대한 Leuconostoc lactis 51의 영향

김태근,양규호,오종석 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.4

치아우식증은 치아구조의 국소적, 침윤적, 분자적인 붕괴로 특징지워지는 치아 경조직에 대한 세균성질환이다. 이런 치아우식증의 주 원인균인 S. mutans의 치태형성과 증식에 대한 아동의 구강에서 분리된 L. lactis 51의 작용을 연구하여 다음과 같은 결과를 얻었다. 1.비커 와이어 검사에서 S. mutans와 L. lactis 51의 혼합배양시 S. mutans 단독배양에 비해 치태의 무게가 감소하였다. 2.S. mutans는 S. mutans와 L. lactis 51의 혼합 배양에 비교하여 S. mutans 단독배양시에 생균수가 감소하였다. 3.S. mutans와 L. lactis 51은 M17Y broth에서 단독 및 혼합배양시 배양 12시간 때까지 증가하다가 24시간 때에 감소하였으나, M17YS broth에서는 S. mutans와 L. lactis 51의 혼합배양시 S. mutans의 생균수가 시간이 지남에 따라 감소하였다. 4.L. lactis 51의 배양 상청액은 S. mutans의 치태형성과 증식에 대해 억제 작용을 하지 못하였다. 5.M17YS broth에서의 L. lactis 51 배양 상청액 성분의 thin layer chromatography에서 자당과 과당이 계속 검출되었다. 이상의 결과를 종합하면 구강에서 분리된 L. lactis 51는 S. mutans의 인공치태 형성과 증식을 억제시키는 것으로 사료된다. Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follow. 1.Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker. 2.Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone. 3.In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51. 4.The culture supernatant of L. lactis 51 didn't inhibit the replication of S. mutans and the formation of artificial plaque. 5. Sucrose and fructose were extracted from the culture supernatant of L. lactis 51 in M17YS broth. Thest results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.

Streptococcus mutans의 치태형성에 대한 Leuconostoc lactis 51의 영향

김태근,양규호,오정석 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

Dental caries is a bacterial disease of the dental hard tisssus, characterized by a localized, progressive, molecular disintegration of tooth structure. The action of Leuconostoc lactis 51 about plaque formation and replication by Streptococcus mutans was studied as follows. 1. Lower amount of plaque was produced at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone on the wires in the beaker. 2. Fewer cells of S. mutans were replicated at the mixed culture of S. mutans and L. lactis 51 than S. mutans alone. 3. In M17Y broth, viable cells of S. mutans and L. lactis 51 increased for 12 hours, and decreased for 24 hours. In M17YS broth, viable cells of S. mutans showed time-dependent decrease at mixed culture of S. mutans and L. lactis 51. 4. The culture supernatant of L. lactis 51 didn't inhibit the replication of S. mutans and the formation of artificial plaque. 5. Sucrose and frutose were extracted from the culture supernatant of L. lactis 51 in M17YS broth. These results suggest that L. lactis 51 isolated from the oral cavity inhibits the replication of S. mutans and the formation of artificial plaque.

측두하악 관절의 재건을 위한 늑연골 이식의 효과

김태규,정기돈,신상훈,김성길,이성근,박상준,서종천 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.3

We performed costochondral grafting in mmandibular reconstruction including condyle replacement in 5 patients which have 4 cases of ameloblastoma, 1 case of resorption of left condyle due to osteomyelitis. We harvested the 6 th costochondral cartilage of unaffected side. No specific complications were appeared with unexpected fracture of costochondral junction and infection of operation site, These patients show 30-45mm at month opening, near normal of affected side, near normal or normal of unaffected side at lateral excursion. Postoperative coureses was uneventful with improved cosmetic and functional results. Our report is indicated that the costochondral graft is satisfactory to reconstruct mandibular defect including condyle replacement, especially in growing patients.