유방종괴의 감별에 있어서 초음파 소견의 진단척도 타당성

한헌,이창근,이경희,김형진,서창해 대한초음파의학회 1998 ULTRASONOGRAPHY Vol.17 No.2

菟絲子 의效能에 대한 硏究

진상식,황의현,김형창,김천수,오광수,한종현 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

The purpose of this study is to determine the effect of Semen Cuscutae on phenylephrine(PE) and KCI induced contraction of isolated rat thoracic aorta. Contractile force was measured with force displacement transducer under 1.5 g loading tension. Contraction evoked by PE (ED_(50)) was relaxed significantly by Semen Cuscutae. The mean percent relaxing of PE induced contraction was 60.6% (p<0.01) after 300 ㎕/㎖ Semen Cuscutae, but the mean percent contraction of KCI induced contraction was 10.4% (p<0.05) after 300 ㎕/㎖ Semen Cuscutae. Vasorelaxation by Semen Cuscutae was significantly reduced after with and without endothelial cells. Pretreatment of aortic rings with L-NAME (100 μM), a nitric oxide synthase inhibitor, indomethacin (3 μM), an inhibitor of cyclooxygenase, methylene blue (10 μM), guanylyl cyclase inhibitor, propranolol (1 μM), a β-adrenoceptor antagonist, atropine (1 μM), a muscarinic receptor antagonist and diltiazem (10 μM), a Ca^(2+)-channel blocker was recovered the effect aorta. These results indicate that Semen Cuscutae can relax EP induced contraction of isolated rat thoracic aorta and that this increasing contraction related to epithelium and various effect.

슬러지 전처리와 고농도 MBR을 이용한 슬러지 감량화 공정연구

한규철,염익태,정우진,김형수 대한상하수도학회 2003 상하수도학회지 Vol.17 No.4

From this research, the performances of a sludge reduction in the sewage sludge aerobic digestion was experimented by using a sludge pretreatment and membrane bioreactor. The submerged plate membrane was used as the solid-liquid separation membrane . After drawing small amounts of sludge in a bioreactor and then doing the alkaline treatment and ozone treatment, the sludge was sent to back to the reactor. The HRT in the reactor was set as 5 days and the operation in the reactor was carried out at the DO of 1㎎/L on average. After 100 days of operation in the reactor, it was shown that the reduction efficiency of total solids was more than 83%. Most of volatile solids were removed through mineralization, and the considerable portion of the nonvolatile solids was dissolved and then flowed out with the effluent. Only about 16.3% of total solids in the sludge was accmulated in the reactor even without the loss of volatile fraction. Also, by deriving nitrification and denitrification in one reactor simultaneously, more than 90% of nitrogen removal effect was realized and the experiment was run smoothly without fouling of membrane, even in the high concentration of MLSS. Based on this experiment. sludge can be reduced considerably at a low HRT by these two newly suggested approach.

CFRP와 콘크리트 계면에서의 부착특성

한상훈,조홍동,박중열,황선일,김형진 충북대학교 건설기술연구소 2002 建設技術論文集 Vol.21 No.2

Externally bonding technique of CFRP(Carbon Fiber Reinforced Polymer Plate) is widely used in strengthening of concrete structures, and the improving effect of flexural strength and stiffness has been proven by early researchers. But, strengthened concrete structures present brittle failure mode accompanied with debonding of CFRP. That is, it means that the behavior of concrete structures strengthened with CFRP is mainly dependent on mechanical characteristics of CFRP and concrete interface. Therefore, double-lap shear test was performed to examine the bond characteristics on interface. The experimental variables are included with the strength of concrete, bond length and width. Test results showed that effective bond length of CFRP is approximately 30cm and maximum interfacial shear stress at specimen failure is 47 kgf/㎠.

인삼복용이 태권도선수의 대사기질 및 호르몬에 미치는 영향

박승한,김진형 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The purpose of this study was to investigate the effect of ginseng supplementation on metabolic substrate(glucose, FFA), metabolic hormone(catecholamine). Fourteen Taekwondo athletes were matched and randomly placed into ginseng(22.9±1.79yrs; n=7) and placebo supplementation(22.4±2.12yrs; n=7). All subjects were either supplemented a standardized ginseng capsule(containing 300㎎ of ginseng in each capsule for ginseng supplementation group) or ingested a placebo capsule(containing 300㎎ of rice powder in each capsule for placebo group) for 4wks. Both capsules used in this study had the same size, weight, shape, and color. Before and after the intervention, each subjects performed incremental cycle ergometer test under controlled laboratory conditions. All exercise sessions took place on a Monark ergometer(@ 50 RPM), performing 20 minutes of cycling at 2kp. After 20minutes, work rate was gradually increased(1kp/every 2minutes) until exhaustion. Blood samples were obtained before and immediately after exercise session. Acute exercise increased blood glucose concentration, but FFA concentration did not induce any significant change. Catecholamine(epinephrine, norepinephrine) concentration increased significantly after immediately exercise. After ginseng supplementation glucose and FFA was observed unchanged. There was no measurable change in epinephrine concentration with ginseng supplementation. However ginseng supplementation increased norepinephrine concentration after immediately exercise. In conclusion acute exercise increase blood glucose and catecholamine concentration. Ginseng supplementation enhanced this exercise induced increase of metabolic hormone. Further study needed to elucidate the exact mechanism with ginseng supplementation. However ginseng supplementation increased norepinephrine concentration after immediately exercise. In conclusion acute exercise increase blood glucose and catecholamine concentration. Ginseng supplementation enhanced this exercise induced increase of metabolic hormone. Furthere study needed to elucidate the exact mechanism with ginseng supplementation and exercise related to muscle glycogen and DNA syntheses.

HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

악성 신경교종에서 기관형적 배양후 형성된 미세종양 구상체의 초미세구조적연구

신형진,정천기,박인성,정영섭,정희원,한대희 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.3

The present study investigated not only the feasibility of organotypic spheroid culture system taken from human malignant gliomas but also the similarities and differences between surgical specimens and cultured spheroids using light microscopy electron microscopy, and flow cytometric examination. Surgically resected tumor specimens from eighteen human malignant gliomas were minced and explanted into agarose-coated culture wells. After three to five days these microtumor fragments emerged as spheroids and could be maintained as organotypic spheroids for more than eight weeks. Measurements of the spheroids showed that they decreased during the initial two to three weeks and afterwards remained unaltered over a specific period of time. This growth pattern of the spheroids was consistent with the condition of tumors in vivo suggesting the linkage of cell proliferation and loss. Light microscopic and electron microscopic studies of the spheroids demonstrated that morphological structures were similar to those of the original tumor tissue in vivo and histopathologic characteristics of the original tumor were maintained over a long culture period. The spheroids contained connective tissues, blood vessels, macrophages and neutrophils maintaining a three-dimensional architectural resemblance to the original tumors. Of three pairs of the surgical and spheroid specimen examined by the flow cytometry, one showed a change of ploidy pattern and two contained increased fictions of proliferating cells. It is concluded that this microtumor spheroid system can maintain the characteristics of the original tumors and may serve as an alternative to the in vivo xenograft model for the research of brain tumor biology, invasion and immunology while providing a valuable technique for the evaluation of new therapies such as biological response modifiers.

Ti-6Al-4V 합금의 표면개질에 의한 calcium phosphate의 형성

박은진,김도균,김교한,고 융부,김형일,정용수 대한치과기재학회 2000 대한치과재료학회지 Vol.27 No.1

The purpose of this study os to investigate the formation of calcium phosphate on Ti-6Al-4V surface on aqueous solutions containing phosphate and calcium ions. The surface of Ti-6Al-4V specimens were treated in phosphate buffered solutions for 3 days followed by immersion in calcium phosphate buffered solutions for 10 days. These solutions have different concentration and pH(pH 5.8, 7.0, 8.0) one another. Then, the specimens treated with P and Ca ions and control group without surface treatment were immersed in Hanks' balanced solution for 30 days to make sure the effectiveness of this surface treatment. All specimens were characterized using scanning electron microscopy(SEM), X-ray diffractometry(XRD) and Fourier transform infrared spectrophotometry(FT-IR). The amount of crystallized particles formed on the specimens by surface treatment was significantly increased in all experimental conditions as compared with that immersed only in Hanks' solution. Dense granular shaped precipitates were observed on the surface and these particles were recognized as calcium phosphate similar to HA(hydroxyapatite) or TCP(tri-calcium phosphate).

백서 중대뇌 동맥의 폐쇄에 따른 경색의 시간적 변화

정진명,정영섭,박인성,이상형,김현집,최길수,한대희 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.3

For the assessment of clinical management, for the confirmation of clinical findings, and also for the evaluation of new diagnostic techniques to determine the location as well as the extent of area of infarction on evolution in cerebral tissue is essential. Sequential evolution of infarction in 2% 2, 3, 5-triphenyltetrazolium hydrochloride(TTC) staining and its concomitant neurological changes were investigated in the rat following left middle cerebral artery occlusion(MCAO). In addition, the pathological evaluation was performed in the same coronal cut slice of each TTC staining. The results were : 1) In the TTC staining method, the cerebral infarction was not found in the 2 hour group rats, and appeared as white or pink color after 4 hours. 2) The size of infarction was significantly correlated with time of occlusion before sacrifice(p<0.05). The size increment was most obvious between 8 hour and 24 hour groups. 3) The time evolution of cerebral infarction was most prominent in the cerebral cortex, and was minimal in the basal ganglia which are supplied by the 'end artery'. 4) The cerebral infarction appeared first in the coronal cuts at the 4, 6, and 8㎜ from the frontal pole, which is the main territory of MCA. 5) The cerebral infarction, mainly presented in the 4, 6, and 8㎜ coronal cuts from the frontal pole, extended from the pyriform cortex to the fronto-parietal cortex. It also appeared at 2, 10㎜ coronal cuts from the frontal pole in 24 hour group. 6) The neurologic sign was not correlated to the time of MCAO and the size of infarction on evolution. Therefore, the prediction of location and size of area of infarction on evolution was impossible by the neurological status. 7) The histopathological change was detected as early as in 2 hours. However, hematoxylin and eosin(H & E) stained sections showed only subtle changes, such as small irregular areas of cortical spongiosis and neuronal shrinkage up to 8 hours. There was no significant difference between lesion areas of 2 hour and 8 hour groups. The pathological findings of 24 hour group rats was definite and appeared as a central area of coagulation necrosis and rare faction surrounded by a zone of peripheral spongiosis.

악성 거대 난소 점액성 종양 1예

신형도,박형진,김홍곤,한원철 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.1

Mucinous tumor are cystic tumors with loculus lined with mucin-secreting epithelium constitute approximately 8-10% of primary epithelial ovarian tumors. Of all mucinous neoplasms, 75-85% are benign. It is often reach very large size particularly with the benign counterpart being reported on occasion to have exceed 100 lbs. Malignancy develops in 5-10% of benign mucinous cysts. About 15-20% of neoplasms are bilateral. We experienced a huge ovarian mucinous tumor of 155 ㎏. measured 42 × 30 × 28 ㎝ in size, and the volume of cystic contents was about 10 L in a nulligravida 36-year-old woman. Pathologic diagnosis was moderately differentiated mucinous cystadenocarcinoma of right ovary. So, we presented with a brief review of literatures.