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백화사설초 메탄올 추출물에 의한 HL-60 세포고사과정에 있어서의 transcriptional factors 활성변화 연구
박상구,이지현,문구,문석재,원진희,박래길 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1
Objective : Hedyotis diffusa has been used as an arnicancer agent for several decades in oriental medicine. We test whether the methanol extract of the herb affects transcriptional activation factors including NF-κB and AP-l. Methods : 1. HL-60 cells were treated with various concentrations(from 200 to 50ug/ml) of methanol extract and H_2O extract(200ug/ml)of hedyotis diffusa, After 48h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with 200ug/ml of methanol extract for the indicated periods. First. Nuclear extracts were isolated and incubated with oligonucleotide probe of NF-κB and AP-l. Second. Nuclear extracts were isolated and reacted with p50, p65. c-rel par-Jun, c-Jun, JunB. JunD antibody on ice for 30min. Finally The cell lnates were prepared and analyzed by westem blotting using anti-Fas, anti-FasL and anti-p53 antibdy. Results : l. The methanol extract decreases the viability of human lymphoid origin leukemia HL-60 cells in a dose-depemdemt manner. 2. NF-κB is rapidly activated by the addition of the methanol extract, reaches a peak at 30min and gradually returns to resting level. We confirm tha NF-κB is a heterodimer mainly composed of p65 subunit with c-Rel. 3. Transcriptional activation of AP-1 is detected at 30min and reaches a maximum at lhr after stimulation of the cells with the methanol extract. AP-l is mainly composed with Jur-D and partially Jug-B proteins. 4. the methanol extract of Hedyotis diffusa induces the expression of Fas, Fas ligand and p53 proteins of HL-60 cells in a time dependent fashion. Conclusions : These results suggest that the methanol extract of Hedyotis diffusa exerts anticancer effects to induce the death of human leukomic HL-60 cells via activation of trascriptional factors such a NF-κB and AP-1, increase in expression of Fas mediated signaling proteins, and induction of tumor suppressor gene. p53
Inhibitory Effect of Hexane Fraction from Myagropsis myagroides on Pancreatic α-Amylase In Vitro
( Won Min Pak ),( Koth Bong Woo Ri Kim ),( Min Ji Kim ),( Ji Young Cho ),( Dong Hyun Ahn ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel column chromatography, and six subfractions were obtained. The fraction eluted with CHCl3:MeOH = 50:1 showed the highest α-amylase inhibitory activity with an IC50 value of 0.72 mg/ml. This fraction was purified using Sephadex LH-20 column chromatography and an octadecyl silica (ODS) Sepak cartridge, obtaining seven subfractions. Fraction (Fr.) 4 also showed a strong α-amylase inhibitory activity with an IC50 value of 0.75 mg/ml. Fr. 4 was purified by Sephadex LH-20 column chromatography and ODS Sepak cartridge, obtaining six subfractions. Fr. 4-2 was identified as sargachromanol I with an IC50 value of 0.40 mg/ml, and the inhibition pattern analyzed from Lineweaver-Burk plots revealed it to be an uncompetitive inhibitor. These results suggest that Mm has potential as a natural antidiabetes agent.
박원민 ( Won Min Pak ),김꽃봉우리 ( Koth Bong Woo Ri Kim ),김민지 ( Min Ji Kim ),박지혜 ( Ji Hye Park ),배난영 ( Nan Young Bae ),박선희 ( Sun Hee Park ),안동현 ( Dong Hyun Ahn ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.1
잔가시 모자반 추출물의 피부 노화 억제 효과를 확인하기 위하여 항산화 활성, tyrosinase 저해 활성, collagenase 저해 활성을 측정하였다. 잔가시 모자반 에탄올 및 물 추출물의 폴리페놀 함량은 11.20 mg/g 및 11.70 mg/g으로 차이가 나지 않았다. DPPH radical 소거능에서는 잔가시 모자반 에탄올 및 물 추출물 모두 0.5 mg/ml 농도에서 83.68% 및 79%로 활성이 높게 나타났으며, 금속봉쇄력에서는 에탄올 추출물이 물 추출물보다 높게 나왔으나 대조구인 EDTA보다 활성이 낮게 나타났다. Tyrosinase 및 collagenase 저해 활성은 에탄올 추출물이 물 추출물보다 더 높은 것으로 나타났고, collagenase 저해 활성은 에탄올 추출물이 1 mg/ml 농도에서 80% 이상으로 높은 효소 저해능을 보였다. 따라서, 잔가시 모자반은 에탄올 추출물에서 항산화능, tyrosinase 및 collagenase 저해 활성이 더 높게 나타나 피부 노화 억제를 위한 소재로서 더 효과적으로 이용될 수 있을 것으로 사료된다. Sargassum micracanthum (SM) is a member of the family Sargassaceae and commonly occurs along the coast of Korea. Extracts from SM were evaluated for their antioxidant and collagenase and tyrosinase inhibitory activities based on their total phenolic concentration (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect, and metal-chelating effect. The TPC of SM ethanol and water extracts used in the study was 11.20 and 11.70 mg/g of dry sample, respectively. Both SM ethanol and water extracts had high DPPH radical-scavenging effect. The metal-chelating effect of SM ethanol extract (40.47% at 0.5 mg/ml) was higher than that of water extract (23.28% at 0.5 mg/ml). With regard to the antiwrinkling effect, SM ethanol extract showed collagenase inhibitory activity with an IC50 value of 488.20 μg/ ml. Lastly, regarding the anti-melanogenesis effect, SM ethanol extract showed higher tyrosinase inhibitory activity (45.08% at 5 mg/ml) than that shown by the water extract (21.29% at 5 mg/ml). These results suggest that SM has the potential to be a resource with natural anti-melanogenesis, anti-wrinkle, and antioxidant effects.
열처리에 의한 Morganella morganii와 Photobacterium phosphoreum 유래 Histidine Decarboxylase의 불활성화
박원민(Won-Min Pak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박지혜(Ji-Hye Park),배난영(Nan-Young Bae),박선희(Sun-Hee Park),안동현(Dong-Hyun Ahn) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3
Histamine을 생성하는 Morganella morganii와 Photobacterium phosphoreum으로부터 crude histidine decarboxylase(HDC)를 추출하여 65~121°C로 열처리한 다음 균의 생육 및 효소 활성 변화에 대해 살펴보았다. 그 결과 M. morganii와 P. phosphoreum은 모든 열처리 조건에서 비 가열 처리구와 비교 시 균의 생육이 저해됨을 확인하였다. M. morganii와 P. phosphoreum 유래 HDC의 효소 활성은 65°C에서 90% 이상의 효소 활성이 저해됨을 확인하였고, 온도가 증가함에 따라 유의적으로 활성이 억제되는 것으로 나타났다. SDS-PAGE 결과에서는 65~100°C 범위에서 비가열 처리구와 비교 시 조효소액의 단백질 패턴의 변화가 크지 않았으나, 121°C에서 단백질 band의 강도가 크게 약해졌다. Native-PAGE에서는 65°C 처리 조건에서부터 단백질 패턴의 변화가 크게 나타났다. 따라서 가열처리(65~121°C)는 histamine 생성균인 M. morganii와 P. phosphoreum의 생육을 억제할 뿐만 아니라 HDC의 효소 활성도가 저해됨을 확인하여, 식품산업에서 적용되고 있는 열처리 조건에서 histamine 생성 억제에 큰 효과가 있는 것으로 생각한다. This study was performed to investigate the effects of various thermal treatments on the growth of Morganella morganii and Photobacterium phosphoreum and activity of crude histidine decarboxylase (HDC) obtained from M. morganii and P. phosphoreum. Crude HDC and the two strains were treated at 65°C/30 min, 80°C/10 min, 100°C/10 min, and 121°C/10 min. Activity of crude HDC decreased with increasing temperature. Viable cells counts of M. morganii and P. phosphoreum were not detected in any heated samples. SDS-PAGE patterns of heated HDC did not show significant differences up to 100°C. However, at 121°C, protein band intensity was weakened. In native-PAGE, there was a major change in the pattern of HDC at 65°C. These results suggest that thermal treatment can help to reduce histamine production by reducing HDC activity and growth of M. morganii and P. phosphoreum.
Pak, Kyung Ho,Hyung, Woo Jin,Son, Taeil,Obama, Kazutaka,Woo, Yanghee,Kim, Hyoung-Il,An, Ji Yeong,Kim, Jong Won,Cheong, Jae-Ho,Choi, Seung Ho,Noh, Sung Hoon Springer International 2012 Surgical endoscopy Vol.26 No.1
<P>Although many reports have indicated the feasibility of laparoscopic gastrectomy (LG) regarding short-term surgical outcomes, the role of LG remains controversial because studies of long-term outcomes of LG are insufficient. The purpose of this study was to evaluate the long-term oncologic outcomes of patients who have undergone LG.</P>