대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

Free Communications : P2 ; Cannabinoid Receptors : Its Impact in Epidermal Differentiation and Possible Role in Treatment of Psoriasis

( Ji Seok Kim ),( Chan Hee Nam ),( Ji Yeon Yoo1 ),( Hyun Jung Kim ),( Se Kyoo Jeong ),( Sung Ku Ahn ),( Seung Phil Hong ) 한국피부장벽학회 2014 한국피부장벽학회지 Vol.16 No.2

Purpose: There are researches indicating that in human skin, the cannabinoid receptors exist, which take a part of endocannabinoid system. Nonetheless, the possible association of cannabinoid receptors with the pathogenesis of psoriasis has not yet been fully elucidated. Through this research, we tried to document the association between cannabinoid system, epidermal differentiation and psoriasis. Methods: Using human keratinocyte (KC), the expression of cannabinoid receptor-1 (CB1R) and -2 (CB2R) was analyzed according to the degree of differentiation. Also we examined for changes in differentiation marker of KC after application of CBR agonist. In addition, murine model applied with imiquimod to induce psoriasis symptoms was used to evaluate the effect of topical CBR agonist on inflammation and skin barrier function. Results: Compared to normal human skin, CBR expression was reduced in epidermis of psoriasis skin. Western blotting revealed the expression of both CB1R and CB2R in undifferentiated KC. The expression level of CB1R increased as the differentiation progressed in KC as with involucrin, K1 and K10. On the other hand, there was no change of CB2R in the process of differentiation. Furthermore, CB1R agonist partly increased expression of proteins associated with epidermal differentiation. Through this we could speculate that the CB system may be associated with proteins related to epidermal differentiation. Moreover, inflammation and barrier function in murine models with psoriasis symptoms were improved after 3 days-application of topical CB1R agonist. Conclusion: Cannabinoid receptors in human skin might be related to the differentiation of KC, and since its activation could improve skin barrier condition in murine psoriasis model, it can be expected to be a new therapeutic target for treatment of psoriasis.

갑상선 종양에서 thrombin receptor (PAR-1)의 발현 양상

박지영,목지오,김여주,박형규,김철희,변동원,서교일,유명희,조현득,오미혜,김상진 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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정신분열병의 임상적 고위험군의 특징 : '서울청년클리닉'의 자연 추적 관찰 예비연구

유소영,이경진,강도형,이승재,하태현,위휘,이애라,송지연,김성년,권준수 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.1

Objectives : The aims of this study were to identify the intake and following process for subjects at high risk of transition to schizophrenia and to examine the neurocognitive, social, and emotional functions of the subjects compared with healthy controls. Methods : Symptomatic individuals judged at high risk for schizophrenia ('clinical high risk') within Youth Clinic were assessed and followed up. They performed a neurocognitive function test, a social function test and an affective function test. Twenty healthy controls were recruited in this study. Results : Among eighty-two subjects contacted through the Youth clinic, sixteen subjects were judged as the clinical high risk group. Fourteen subjects among the clinical high risk group showed deficits in several domains of neurocognitive functions, such as visual recall memory, verbal short term memory and executive function. Social and affective functions are also impaired in the Cli-nical high risk group compared with healthy controls. Two of 15 subjects (13%) developed a psychotic disorder within 6 months. Conclusion : This study illustrates the state of follow-up study for a clinical high risk group. Despite low numbers and short durations, some impairment of several functions in the clinical high risk group suggests possible predictors of psychosis.

국산 Avana 인공치아와 외국산 ITI 인공치아의 악골조직내 적합성에 관한 비교연구

유재하,이지웅,백성흠,정원균,김종배 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

The purpose of this article is to confirm the longterm biocompatibility of national Avana implant and international ITI implant in dogs. At the fourth month after extreaction of the mandibular third and fourth premolar in three dog, both implants were inserted using careful atraumatic technique and prosthetic abutments were applied onto the implants in three months. After that, the implants were examined by naked eye and radiographic image every three months during 1 year, for detection of the complications, such as, mobility, bone resorption and wound infection. At 1 year after application of prosthetic abutment, the dogs were sacrificed for the histopathologic analysis by light microscope. The results obtained were as follows; 1. All implants had the ralatively good prognosis without mobility, wound infection and moderate bone resoprtion, in clinical and radiological evaluation during one year. 2. The all histopathologic specimens at one year after application of prosthetic abutment onto the implants showed the distinct osseointegration between implant system and bone, with projection of new bone apposition onto the peri-implant tissue. But Avana implants had the less osseointegration than ITI implants in the cancellous and marrow layer of jaw bone. 3. The all histopathologic specimens at one year after application of prosthetic device showed the firm junction by dense connective tissue pattern between the implant and the gingival portion. But Avana implants had the less firmer junction than ITI implants. 4. The authors confirmed that Avana implant and ITI implant have more higher tissue biocompatibility in dental implantation, but ITI implant has the more osseointegration than the Avana implant.

야간응급실로 내원한 급성 치통환자의 질환특성에 관한 임상적 연구

유재하,최병호,민연숙,오민성,이지응 연세대학교 원주의과대학 1999 원주의대논문집 Vol.12 No.1

혈청이 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

유지창,정헌택,김유현,진효상,김신무,배현옥 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1999 Journal of biomedical laboratory sciences Vol.5 No.1

마우스 간 세포주인 BNL CL.2의 시험관내 배양에서 혈청과 IFN-γ가 세포주의 nitric oxide(NO) 생성과 세포 손상에 미치는 영향을 알아보기 위한 실험을 하였다. 혈청이 공급된 배양에서 IFN-γ에 의한 세포 생존율은 거의 변동이 없었으나, 혈청을 제거한 배양에서는 약 65%의 생존율이 유지되었으며, NO 생성 억제제인 N -monomethy-L-arginine (NMA)의 첨가는 농도 의존적으로 세포의 생존율을 감소시켰다. 혈청이 제거된 BNL CL.2세포주는 IFN-γ단독 처리에서도 NO 를 생성할 수 있었으며, IFN-γ와 lipopolysaccharide (LPS)의 복합 처리는 세포주의 NO 생성을 상승적으로 증가시켰다. 또한 protein tyrosine kinase (PTK) inhibitor인 herbimycin A와 genistein에 의해서 NO 생성이 억제되어 PTK의 활성이 혈청이 고갈된 BNL CL.2 세포에서 NO의 생성에 중요한 역할을 담당하고 있기 때문으로 판단된다. IFN-γ의 독성은 혈청을 제거시킬 때 NO 생성 억제제에 상승적으로 간 세포를 손상시키며, 이때 NO가 IFN-γ에 의해 유도된 손상을 어느 정도 억제시키는 것을 알 수 있었다. Nitric oxide (NO) plays an important role in immunologic defense, and influences upon the functioning of secretory tissues and cells. It also exhibits cytotoxic/cytostatic activity as one of major operating effectors of the cellular immunity system. We investigated the effects of serum on the cell damages and NO production in the mouse liver cell line BNL CL.2 to establish the role of NO. We observed that, when BNL CL.2 cells were cultured in serum-free medium, they were induced to cell damage by the stimulation of IFN-γalone or IFN-γplus LPS. Serumstarved cells showed large amount of nitrite accumulation and NO synthase (NOS) expression in response to IFN-γalone in dose-and time-dependent manners, but serum-supplied cells did not. The production of NO was blocked by protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin. These results suggest that the deprivation of serum in the BNL CL.2 cell culture medium might primed with the cells to produce NO when the cells are triggered by IFN-γ and the involvement of PTK signal transduction pathway in the expression of NOS gene in murine hepatocytes.

胎盤組織 Guanine Aminohydrolase에 關한 硏究

유지헌,林圭,郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

Guanine aminohydrolase(EC 3.5.4.3, GAH) has been purified from human term placenta approximately 1,280-fold with a 10% yield by combination of ultracentrifuge, ammonium sulfate precipitate, Sephacryl S-300 chromatography, calcium phosphate gel adsorption, DEAEcellulose chromatography and hydroxyapatite chromatography, and then its properties were investigated. 1. The purified enzyme appeared single protein band by SDS/polyacrylamide gel electrophoresis. 2. The enzyme showed a pH optimum around 5.6-8.6. It was heat labile, inactivated by heat-treatment for 10 minutes at 70℃, and irreversively denatured by freezing and was stable at 4℃ for at least 1 month. 3. Copper ion (Cu^2+) was a potent inhibitor, the activity being inhibited upto 75% at 2 mM concentraion and the inhibition was completely recovered its activity by additon of 4 mM EDTA, and zinc ion (Zn^2+ also inhibited upto 40% at 2 mM concentration. The other ions did not almost inhibit. 4. The apparent Km value for guanine was 4 X 10^-5M, and the enzyme activity was completely inhibited by 0. 5 mM of p-hydroxymercuribenzoic acid, but all of the activity was recovered in adding 10 mM mercaptoethanol. 5. The molecular weight of the enzyme showed 170,000 by Sephacryl S-300 chromatography, and 55,000 by SDS-polyacrylamide gel electrophoresis. And the properties such as above results of guanine aminohydrolase of human term placenta are compared with those of guanine aminohydrolase of other tissues.

실천적 문제 중심의 고등학교 가정과 교육과정 개발에 관한 연구 : 식생활 내용을 중심으로

유지연,신상옥 한국 가정과 교육 학회 1997 한국가정과교육학회지 Vol.9 No.1

The purpose of this study was to develop a Home Economics curriculum for high school students based on practical problems focusing on Food and Nutrition. This study developed practical-problem based approach to curriculum development. This curriculum provided a series of the objectives, contents, learning activities, and assessments. The objectives were divided into process objectives and content objectives. The objectives focused on three systems of action which were technical action, communicative action, and emancipatory action. The contents of this curriculum focused on three practical problems ; 1) What should I do regarding my well-being? ; 2) What should I do regarding my family's well-being? ; 3) What should I do regarding my society's well-being? The cooperative learning strategies were mainly used in this curriculum. Students were assessed through learning experiences and achievement.

후두부 발작성 간질파와 동반된 양성 소아기 간질의 임상적 연구

유영수,이상도,김지언,임정근,박영춘 대한신경과학회 1996 대한신경과학회지 Vol.14 No.4