天然우라늄 毒性에 關한 治療硏究

유용운,이진오,윤택구 대한방사선 방어학회 1987 방사선방어학회지 Vol.12 No.2

천연 우라늄의 주독성이 중금속독성인지 혹은 방사성장해독성인지를 알기 위하여 질산납과 질산우라늄을 투여하여 변동되는 혈중 BUN, Creatinine C-AMP 및 PGE2의 활성도를 측정 비교하였다. 질산우라늄 투여시 비효소계인 질산대사의 임상적 지표인 BUN 및 Creatinine값은 질산납 투여군에 비교하여 예민한 반응을 나타냈으나 C-AMP의 활성도에는 의의있는 변화가 없었다. 한편 질산 우라늄의 농도 변화에 따른 PGE2의 활성도의 변동은 질산납 투여와 달리 현저하게 증가 되는 것을 관찰하였다. 이 결과는 천연우라늄의 저준위 방출 방사선이 세포막에 반응하여 PGE2농도에 변화를 주는 것으로 사료된다. 질산 우라늄의 투여로 증가된 혈중 PGE2의 농도를 감퇴시킴에 있어 Glucagon, Aldosterone 및 Furosemide를 사용하였다. The present study has determined BUN, createinine, c-AMP and PGE; activities as a clinical signs of radiation toxicity caused by uranylnitrate in rats. The significant increasing of PGE; concentration in plasma between the administration of uranylnitrate and lead nitrate were shown radiotoxic in nature on the effect of radiation energy. The reduction of PGE activities in plasma in uranylnitrate treated rats after furosemide, aldosterone and glucagone I.P. administration have observed the stimulating effect of uranium excretion into cells.

사람폐암세포주 (PC-14)에서 Cyclosporin A에 의한 Adriamycin 내성의 극복

김영환,홍원선,송재관,강윤구,이진오,강태웅,김건열,한용철 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Cyclosporin A and verapamil were tested using MTT assay to evalute the modification effect on the resistance to adriamycin in a human lung cancer cell line(PC-14) and its resistant subline(PC-14/A). PC-14/A was derived by the continuous exposure of PC-14 to incremental concentrations of adriamycin. PC-14/A was 2.5 times more resistant to adriamycin in terms of ICso than PC-14. Cyclosporin A alone, at a concentration of 2.5㎍/㎖, inhibited the growth of PC-14 to 68.3%. 2.5㎍/ ㎖ and 5.0㎍/㎖ of cyclosporin A showed an increase in the cytotoxicity of adriamycin (p<0.01) with 5.0㎍/㎖ being greater than 2.5㎍/㎖(p<0.01). Excluding the direct cytotoxic effect, however, cyclosporin A did not increase in the sensitivity of PC-14 to adriamycin but only showed an additional cytotoxic effect with adriamycin. Verapamil (up to 6.0㎍/㎖) did not inhibit the growth of PC-14. 3.0㎍/㎖ of verapamil did not increase the cytotoxic effect of adriamycin. The combination of cyclosporin A and verapamil with adriamycin enhanced the cytotoxicity of adriamycin, but the result was similar to that of cyclosporin A with adriamycin. 5.0㎍/㎖ of cyclosporin A modified the adriamycin resistance of PC-14/A(SR, 3.2). However, 3.0㎍/㎖ of verapamil did not significantly reverse the adriamycin resistance of PC-14/A. The modified effect of the combination of 5.0㎍/㎖ of cyclosporin A and 3.0㎍/㎖ of verapamil was similar to that of 5.0㎍/㎖ of cyclosporin A alone in PC-14/A. These results demonstrate that cyclosporin A has an additional cytotoxic effect with adriamycin in PC-14 and PC-14/A and has overcome the acquired resistance to adriamycin in PC-14/A. They also suggest that cyclospoin A may have the therapeutic potential in the treatment of human lung cancer.

위암세포주에서 Recombinant Human Interferon-r와 Adriamycin의 투여순서가 항암효과에 미치는 영향

홍원선,손영숙,김창민,강윤구,이춘택,김유철,임영혁,남현석,이진오,강태웅 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

Numerous previous studies, both in vitro and in vivo, have demonstrated that the cytotoxicity can be enhanced by the combination of chemotherapeutic agent and interferons(IFNs) in various types of cancer cells. We have previously reported that combined treatment of MKN-45, human gastric adenocarcinoma cells, with adriamycin(ADM) and recombinant human interferon-r(rh-IFN-r) increased in the cytotoxicity. In this study, the effects of combination timing of rh-IFN-r and ADM on the cytotoxicity against MKN-45 were investigated using MTT assay. MKN-45 was treated with rh-IFN-r and ADM in vitro on three schedules : Treat A ; rh-IFN-r and ADM were treated simultaneously, Treat B ; rh-IFN-r was treated 24 hours after the treatment with ADM, Treat C ; rh-IFN-r was treated for 72 hours and followed by the treatment with ADM. The survival of MKN -45 was inhibited by ADM dose-dependently. 102 and 103U/ml of rh-IFN-r significantly inhibited the survival of MKN-45(% survival : 35.1 ±-1.2% and 34.4 ±1.1% in Treat A and 42.5 ± 2.1% and 45.9-±2.5% in Treat C, respectively). However no difference in the survival was observed between 102 and 103U/ml of rh-IFN-r. Combined treatment with rh-IFN-r and ADM significantly augmented the cytotoxicity at low concentrations of ADM. Combined effects of rh-IFN-r and ADM were evaluated using IC30(,ag/ml) to ADM. IC30s of MKN-45 in Treat A, B and C at 102 U/ml of rh -IFN-r _ were 0.019 -?- 0.003, 0.045 :I:0.001 and 0.054 ± 0.012, respectively, while IC30 of MKN-45 treated with ADM alone was 0.052±0.004. IC30s of MKN-45 in ADM alone group, Treat A, Treat B and Treat C at 103U/ml of rh-IFN-r were 0.047 ±0.003, 0.004 -±0.001, 0.031 ±0.004 and 0.056 0.008, respectively. These results indicate IC30s of Treat A and B were significantly lower than those of ADM alone(p<0.05) and IC30s of Treat A was significantly lower than those of Treat B(p <0.01). IC30s of Treat C, however, were not different from those of ADM alone. From these results demonstrating that cytotoxic effects were increased by the combination of rh-IFN-r and ADM in the order, Treat A > Treat B> Treat C, it can be concluded that the simultaneous administration of rh-IFN-r and ADM may be the most effective method to combine these two therapeutic modalties.

진행 위암환자에서 Lymphokine-Activated Killer (LAK) 활성의 저하

홍원선,김영환,송재관,강윤구,이진오,강태웅,김정룡 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Natural killer(NK) and lymphokine-activated killer(LAK) activities were determined in 31 patients with unresectable stomach cancer before and immediately after chemotherapy with 5-fluorouracil, adriamycin and mitomycin C(FAM) and 31 healthy volunteers. The major purposes of the study were focused on whether peripheral blood lymphocytes(PBL) in stomach cancer patients had a similar ability in the generation of LAK activity to those in healthy volunteers and what the effect was of chemotherapy with FAM on the generation of LAK activity. LAK cells were generated in vitro by culturing human PBL with 100 U/㎖ of recombinant human interleukin-2(rH-IL-2) for 72 hours. K562(human myelogenous leukemia cell line) and MKN-45(human stomach adenocarcinoma cell line) were used as target cells for NK and LAK activities. NK activity against K562, a sensitive line, was significantly depressed in patients with stomach cancer compared with that in healthy volunteers(p<0.01). However, LAK activity against K562 was similar to that of the controls. Age, sex and performance status(ECOG 0-2 and 3-4) did not have an influence on both NK and LAK activities. LAK activity was significantly higher than NK activity, either against K562 or MKN-45, in both healthy volunteers and patients with stomach cancer(p<0.001). NK and LAK activities against MKN-45, a NK-resistant line, in patients with stomach cancer showed significantly lower levels than those in healthy volunteers. We also analyzed the effect of chemotherapy with FAM on NK and LAK activities, with no suppression of NK and LAK activities being observed. In this study, we have demonstrated that PBL of stomach cancer have a reduced ability to generate LAK activity in response to rH-IL-2. However, LAK activity generated from PBL receiving chemotherapy with FAM was similar to that of PBL without chemotherapy.

Prednisolone 이 급성간염환자의 간기능 및 혈청철치와 동치에 미치는 영향

이진오 ( Jhin Oh Lee ) 대한내과학회 1966 대한내과학회지 Vol.9 No.11

기관지 내시경 검사상 발견된 2부위의 기관지 병변에 관한 연구

이창희 ( Chang Hee Lee ),윤종길 ( Jong Kil Yoon ),곽영임 ( Young Im Kwak ),김현각 ( Hyun Kag Kim ),이춘택 ( Choon Taek Lee ),이진오 ( Jhin Oh Lee ),강태웅 ( Tae Woong Kang ) 대한결핵 및 호흡기학회 1994 Tuberculosis and Respiratory Diseases Vol.41 No.3

HBsAg 양성인 비호즈킨 림프종 환자에서 항암 화학요법 후 간 기능 악화

김창민,이영우,이상원,김봉석,이군희,조진아,정숙향,이진오,한철주,김유철,정문관,강윤구,류백렬,곽진영,홍현주,이군학 대한소화기학회 2000 대한소화기학회지 Vol.35 No.1

Background/Aims: Immunosuppressive therapy such as anti-cancer chemotherapy is often complicated with reactivation of hepatitis B. We tried to investigate the prevalence of hepatitis B virus (HBV) infection in patients with non-Hodgkin's lymphoma (NHL). Furthermore, we evaluated the frequency of hepatic complications, the clinical outcome, and the risk factors associated with the development of hepatic complications in HBsAg-positive patients treated with chemotherapy for NHL. Methods: One hundred eighty-five patients who were diagnosed as NHL and treated with chemotherapy were retrospectively analyzed. Results: The prevalence of HBV infection was 14.1% of the patients. The frequency of hepatic complications in HBsAg-positive patients receiving chemotherapy for NHL was significantly higher than those of HBsAg-negative patients. (50% vs. 10.1%), and the complete response (CR) rate was significantly lower (42.3% vs. 67.9%). We could not find any significant risk factor associated with the development of hepatic complications in HBsAg-positive NHL patients received chemotherapy. Conclusions: For HBsAg-positive NHL patients receiving chemotherapy, special concern and close follow-up is needed because of high frequency of hepatic complication and low CR rate caused by chemotherapy.

Hepatitis C Virus 감염 환자에서 Hepatitis B Virus 중복 감염이 간세포암 발생에 미치는 영향

김창민,이영우,이상원,이은주,조진아,정숙향,이진오,한철주,김유철,곽진영,홍현주 대한소화기학회 2000 대한소화기학회지 Vol.35 No.1

Background/Aims: Korea is an endemic area of hepatitis B virus (HBV) infection. The routes of HBV and hepatitis C virus (HCV) infection are similar. The purposes of this study were to evaluate the effect of HBV infection on the development of anti-HCV-positive hepatocellular carcinoma (HCC) and to study the pattern of viral interaction in the patients with dual infection of HBV and HCV. Methods: One hundred thirty-seven consecutive anti-HCV-positive patients were divided into two groups: HCC group (N=58) and Non-HCC group (N=79). Various clinical, serological parameters and viral replicative status reflecting serum HBV DNA and HCV RNA positivity were compared between two groups. Results: According to multiple logistic regression analysis, male and positivity of HCV RNA, HBsAg, and HBV DNA were statistically significant variables associated with the development of HCC. The mean age at the diagnosis of HCC in the patients with dual infection was significantly younger than that of patients with HCV alone. Among 13 patients with dual infection, one third showed co-replication of HBV and HCV, but two thirds revealed only one viral replication. Conclusions: HBV infection seems to increase the risk of HCC in the anti-HCV-positive patients. To elucidate the complex profiles of viral interaction between HBV and HCV, further study may be needed.

Cyclophosphamide 를 포함한 항암화학요법 후 발생한 간질성 폐렴 3 예

김경태,박연희,이춘택,이승숙,이진오,강태웅,강윤구,남승모,류백렬,임영혁,김태유,성주병,이영우,장은정,허남현 대한내과학회 1997 대한내과학회지 Vol.53 No.4

Development of diffuse pulmonary infiltrates in patients receiving chemotherapy is a major diagnostic challenge. Diffuse pulmonary infiltrates may be due to infection, pulmonary hemorrhage, pulmonary edema or drug-induced lung injury. Among these, pulmonary toxicity caused by antineoplastic agent is being recognized more frequently. Cyclophosphamide, an alkylating cytotoxic drug, is used widely in the treatment of malignancies including lymphoma. The incidence of pulmonary toxicity is probably less than 1 percent, and its relation with total dosages and schedule of the drug is not yet defined. The typical pictures of cyclophosphamide-induced pulmonary toxicity are non-productive cough, dyspnea, fever, hypoxemia with respiratory alkalosis and interstitial pneumonitis. However, relatively infrequent pulmonary toxicity of cyclophosphamide and frequent development of infectious pulmonary infiltrate in the patients treated with chemotherapy may hamper the early diagnosis of cyclophosphamide toxicity. Interstitial pattern and unresponsiveness to antibiotics of the pneumonitis might be the clues of suspicion. The best ways to treat the patients with cyclophosphamide toxicity are early diagnosis, discontinuation of the drug and early corticosteroid trial, although usefulness of steroid has not been firmly established. Recently, we experienced three cases of interstitial pneumonitis developing during cyclophosphamide-containing chemotherapy for non-Hodgkin's lymphoma in the absence of neutropenia or thrombocytopenia. Early use of corticosteroid in later two cases could resolve the pulmonary complication completely, whereas the pneumonitis failed to improve in spite of the massive use of multiple antibiotics in the first case.

형광 [카메라] 상에 의한 정상간의 전면형태에 관한 연구 (예보)

이장규,이진오,김종설,장고창,박종인 대한핵의학회 1975 핵의학 분자영상 Vol.9 No.1

교질형방사성금과 dynacamera를 이용하여 정상성인남자의 간전면상을 얻고 이 중 250예에 대하여 새로운 분류기준으로써 총 9가지 종류로 간전면을 형태적으로 분류하여 다음과 같은 결론을 얻었다. 1) 제 5형(2-2)이 47.2% 차지하였으며, 다음이 제 2형(1-2)과 제 6형(2-3)이 각각 16%와 15.6%를 차지하고 제 3형(1-3)이 0.8%로 가장 적었다. 2) 비만이 차지하는 율은 제 1형(1-1), 제 4형(2-1) 및 제 8형(3-2)에서 높았다. 3) 현저한 cardiac impression은 제 7형(3-1), 제 8형(3-2), 제 4형(2-1)순으로 많았고 사각양좌엽은 제6형(2-3), 제 9형(3-3), 제 2(1-2)순으로 많았고, 미 양좌엽은 제 7형(3-1), 제 8형(3-2), 제 5형(2-2)순으로 많았다. $quot; A knowledge of the wide ranges in shape of liver is important in the interpretation of hepatic scintigraphy as some variants may be easily confused with pathological abnormalities. In this study, the variations in shape in 250 cases of normal liver obtained by scintiphoto were categorized into 9 types by means of the arbitrary standard for classification. 1) Among 250 normal cases, type 5 was 118(47.2%) with the highest prevalence and type 3 was 2(0.8%) with the lowest. 2) A higher incidence of obesity was noted in type 1, type 4 and type 8. That of ideal weight was noted in type 2, type 5 type 6 and type 9. 3) A high ratio of cardiac impression in scintiphoto was noted in type 4, type 7 and type 8. That of square left lobe was noted in type 2, type 6 and type 9. Finally that of tailed left lobe was in type 4, type 7 and type 8.