연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

초등학생들의 물질 개념에 관한 연구

김석중,남철우,김정길,송판섭,한광래,최도성,김숙희 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

Over the past 20 years, research on students' conceptual development of science concepts has begun to inform educators about the roles that prior knowledge and experiences play in the development of conceptual understandings. Whereas many initial studies focused on identifying and cataloging naive of misconceptions, more recent research has focused on how student concept development research can inform teaching practice and curriculum design. In this study, we investigated children's (ages 11∼12) spontaneously constructed or naive understanding of the particulate nature of matter prior to any formal instruction in the domain. Thirty students were interviewed concerning their understanding of the macroscopic and microscopic properties of the states of matter(solid, liquid, and gas), as well as their macro/microscopic understanding of phase changes and dissolving. Children expressed ideas about states of matter which were categorized as macrocontinuous, macroparticulate, or microparticulate. Furthermore, a substantial number of the children provided explanations of properties and processes which were consistent with those beliefs. These children's beliefs about matter were not fully and consistently developed across the spectrum of substances from continuous solids to particulate solids to liquids to gases. We speculate that children first develop local frameworks particular to different classes of substances and then slowly expand these frameworks to include a wide range of substances and their properties as well as such processes as melting and freezing.

척수를 손상시킨 후 꼬리정맥에 주입한 사람탯줄혈액세포가 뇌줄기에 미치는 영향

김종중,정윤영,박영란,문영민,현영식,정영욱,문정석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background and Objectives: Stem cells are a valuable resource for treatment of many disease, but limited access to stem cells in some organs such as brain restricts their utility. Many approaches have been attempted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood cells (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Mateiials and Methods: Forty rats were divided into 8 groups: (1) SCI l+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (mfusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy+hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunhistotochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord injury using mouse anti-human mitochondria monoclonal antibody (MAB1273). Results: All SCI+hUCB (1-8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. But especially a large number of fluorescent hUCB positive cells were observed in the whole area of the brain stem of the experimental 5 (SCI 5+hUCB) and 6 (SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem of group with non-injured spinal cord of these animals and group with laminectomy only. Conclusion: These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous of stem cell study will give an best opportunity to treat the uncurable disorders in the future.

유니사이클 로봇의 곡선경로 추종에 대한 퍼지규칙베이스 구축

김중완,정희균 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

Our unicycle has simple mechanical structure. But unicycle's dynamic system is a very sensitive unstable nonlinear system. In this paper, a fuzzy inference control mechanism was established throughout an inquiry into human riding a unicycle, and we developed a fuzzy hybrid controller to control our unicycle robot. This proposed fuzzy controller os consisted with direct fuzzy logic controllers and fuzzy gain scheduled PD controller for attitude stability and wheel's velocity individuslly. Computer simulation results show that our fuzzy controllers have robust control performance to unstable nonlinear unicycle robot system.

제2형 당뇨병 환자에서 Voglibose와 Acarbose의 비교임상연구

정인경,정재훈,민용기,이명식,이문규,김광원,정윤이,박중열,홍성관,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:아카보스와 보글리보스는 ­glucosidase inhibitors로써 비록 약리학적 작용이나 부작용에 있어서 두 약물간에 차이가 있다는 것은 잘 알려져 있으나 당뇨병 환자를 대상으로 아직 이에 대한 두 약물간에 직접적인 비교에 대해 연구된 바는 없었다. 이에 저자등은 국내 2형 당뇨병 환자에 대해 유효성과 부작용 발현에 대해 두 약제를 비교하고자 무작위법에 의한 위약 대조군의 이중 맹검법 연구를 시행하였다. 방법:시험 약제 투여 4주간의 관찰기를 설정하여 공복혈당의 변화가 30㎎/dL 이하이고, 식후 혈당이 200㎎/dL 이상인 환자로 기타 제외 기준에 해당하지 않은 환자 53명을 대상으로 하여 보글리보스 군(24명)과 아카보스 군(29명)으로 무작위로 나누었다. 치료기간은 총 8주로 하였으며, 4주간 간격으로 혈청학적 검사와 부작용을 분석하여 치료 효과가 부작용을 평가하였다. 결과:1)혈당 변화:보글리보스군은 식후 1시간 혈당이 치료 후 4주, 8주째 의미 있게 감소하였도, 아카보스군은 식후 1시간과 2시간 혈당이 치료 후 4주, 8주째 의미있게 감소하였다. 또한 관찰기 혈당에 대한 치료 4주째 감소량은 아카보스군에서 더 큰 경향을 보였으나, 치료 8주째에는 두 군 간의 강하정도에 의미있는 차이가 없었다(p=0.569). 2)인슐린 치의 변화:보글리스 군은 식후 1시간 인슐린 치가 치료 전에 비해 치료 4주, 8주째 감소하는 경향을 보였고, 공복 인슐린이나 식후 2시간 인슐린치는 치료전 후에 의미 있는 차이가 없었다. 아카보스군에서는 치료 전후로 공복 인슐린, 식후 1시간과 2시간 인슐린치에 의미 있는 차이를 보이지 않았다. 두약제 간에 치료 전과 치료 8주사이의 식후 2시간 인슐린의 감소량이 보글리보스 군에서 의미있게 높았다(p=0.040). 3)당화혈색소:보글리보스 군은 치료 전에 비해 치료후 당화혈색소가 감소하는 경향을 보였고, 아카보스군은 치료 전에 비해 의미있게 감소하였다. 당화혈색소 변화량은 두 군간에 의미 있는 차이는 없었다(p=0.412). 4)지질대사의 변화:중성지방, 콜레스테롤, 고밀도 진단백 콜레스테롤에 대해 두 군간에 의미 있는 차이는 없었다. 5)부작용:소화기계 부작용의 빈도는 치료 4주째 보글리보스 군에서 의미 있게 낮았으나(p=0.028), 치료 8주째 부작용의 빈도는 두 군간에 의미 있는 차이가 없었다(p=0.215). 결론:2형 당뇨병 환자에서 보글리보스와 아카보스의 두 약제의 임상적 유효성과 부작용발현에 대해 비교한 결과 치료 후 8 주 후 혈당강화효과는 두 약제간에 유사한 효과를 보였으나 보글리보스군에서 4주째의 초기 위장관 부작용이 적었다. Background : Acarbose and voglibose are alpha-glucosidase inhibitors. Although different pharmacological effects and adverse abdominal events associated with the two drugs have been reported, no study directly compared acarbose and voglibose in diabetes has been undertaken. To compare the pharmacological effects and gastrointestinal adverse events between two drugs, a randomized, placebo-controlled, double-bind study was performed in type 2 diabetes patients. Methods : The period of study was 12 weeks(observation period: 4 weeks; treatment period: 8 weeks). Fifty-three patients were randomized into two groups(the acarbose group: 24 patients; the voglibose group: 29 patients). The serum glucose, insulin, fructosamine, HbA_1c, cholesterol, triglyceride and the incidence of adverse events were measured. Results : 1) The reduction of glucose from before treatment to 4 weeks after treatment was significantly higher in the acarbose group, but the change before treatment and 8 weeks after treatment in the two groups was similar(p=0.569). 2) The insulin significantly decreased after voglibose treatment(p=0.040). 3) HbAa_1c level tended to decrease in voglibose group, and there was a significant decrease after acarbose treatment. However, the change in HbA_1c level before and after treatment was similar between the two groups(p=0.412). 4) The two drugs did not cause any other changes in the total, HDL-cholesterol and triglyceride. 5) The number of patients with gastrointestinal adverse events was significantly low 4 weeks after voglibose treatment (p=0.049), but the incidence in the two groups was similar after 8 weeks(p=0.215). Conclusions : Acarbose and voglibose significantly improved postprandial hyperglycemia in diabetes. The incidence of gastrointestinal adverse events was low 4 weeks after voglibose treatment(J Kor Diabetes 26:134~145, 2002).

흰쥐 척수 손상후 Parvalbumin과 Calbindin D-28K 함유신경세포에 관한 면역세포화학적 연구

김종중,정윤영,임유택,박상수,박영란,김현곤,문정석 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.2

Background and Objectives: This study was done to compared the distribution of the two calcium-binding proteins immunoreactive neurons, parvalbumin immunoreactiv (PV-IR) and calbindin D-28K immunoreactive (CB-IR) neurons in the spinal cord after transection. Materials and Methods: Twenty rats were divided into 3 groups (control, complete spinal cord injury (SCI), and right and left spinal cord hemisection). SCI was produced by cutting the spinal cord use blades 11 with scalpel handles. Results: In this experiment, CB-IR neurons were mainly found in many pyramidal cells distributed in the brain stem and spinal cord of rats. PV-IR Neurons were demonstrated in all lamina of the gray matter of the spinal cord. These immunoreactive cells had the highest density in the layer I and II of dorsal horn and several nuclei of the ventral horn of the all the segments of the spinal cord. CB-IR neuropil labeling was strongly noted in all the segments of the spinal cord. In contrast PV-IR neurons were different in distribution, size and morphology in the spinal cord. The number of PV-IR neurons were greater than in the spinal cord compared with the CB-IR neurons. CB-IR and PV-IR somata were round, oval, spindle and polygonal in shape, and were unipolar, bipolar, multipolar and horizontal in types. The diameters of the somata of the PV-IR and CB-IR neurons were 40-50 ㎛, respectively. Also dendrites of PV-IR and CB-IR neurons were densely arrayed in network.

유니사이클 로봇에 대한 인간적 추론 제어 메카니즘

김중완,안찬우,전태옥,전언찬,정희균 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

Our unicycle has simple mechanical structure.But unicycle's dynamical system is a very sensitive unstable nonlinear system. equations of motion for a simple unicycle robot were derived using Lagrage's method. In this paper, a human fuzzy influence control mechanism was established throught an inquiry into human riding a unicycle, and we developed a hybrid controller to control our unicycle robot. This proposed hybrid controller is consisted with a pure PD and two fuzzy gain scheduled controllers and a direct fuzzy logic controller. Computer simulation results show that our hybrid controller has robust control performnance to unstable nnonlinear unicycle robot system.

어류 성장에 있어서 식물 및 동물플랑크톤의 먹이기여도

김백호,김미연,최환석,김정중,최민규,박승택,정연태,高村典子 원광대학교 환경과학연구소 2000 環境科學硏究誌 Vol.9 No.1

생쥐모델에서의 망막모세포종 안구 자기공명분광법과 병리소견 : 초기경험

김동훈,김일중,양재한,김정훈,유영석,이봉재 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Purpose: To show various MR spectroscopy and correlated pathologic images of retinoblastoma in nude mouse with a new human retinoblastom cell line (SNUOT-Rbl) which innoculated into intravitreous cavity. Materials and Methods: The established cell line was innoculated into mtravitreous cavity of 14 eyeballs of 7 mice and a transplanted retinoblastoma was examed for 1 month. Tl-weighted image (T1WI), T2-weihgted image (T2WI), and MR spectroscopy were obtained with 1.5 Tesla MRI (Magnetom Vision, Siemens, Erlangen, Germany) and 4.7 Tesla MRI (Bruker, Medizintechnik GmbH, Karlsruhe-Ettlingen, Germany). After scanning, eyeballs were extracted and hematoxylin & eosin stained specimens were examined with the microscope. We compared MR spectroscopy with pathologic findings and evaluated characters of tumors. Results: Innoculated cells into eyeball of mice grew retinoblastoma (8/14, 57%). Eyeball with retinoblastoma protruded externally and showed focal hemorrhage. Tumors showed low (n=3), iso (n=4), high (n=1) signal intensity on TIWI, low (n=5), iso (n=1), high (n=2) signal intensity on T2WI, and well enhancement (n=8) with contrast, Involvement of optic nerve was suspected by MRI and confirmed by pathology (n=1). Conclusion: We could demonstrate MR spectroscopy of transplanted retinoblastoma by new tumor cell line in vivo. This imaging technique facilitate molecular imaging investigation of retinoblastoma.

경상남도 일부지역 농업인의 농약관련 인지도 및 실행도 결정요인

김정민,전만중,고광욱,유병철,이용환 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Background : The amount of pesticide to be produced and consumed have rapidly growed in Korea and the evidences for pesticide intoxication to human body, natural environment and consumers have evoked. But the studies concerned to pesticide intoxication are inactive. So, we conducted this study about the determinants of awareness and performance for prevention of pesticide intoxication. Methods : The study conduced during the period from July to August 2007 with population consisted of 111 farmers. The toolkit was produced through referencing previous studies. Data analysis was performed with t-test, ANOVA and multiple linear regression analysis using SPSS 14.0K program. Results : The mean awareness score was 3.07 ± 0.75, the mean of total performance score was 2.72 ± 0.69, mean of subtotal performance score for keeping safety guideline was 2.72 ± 0.69 and mean of subtotal performance score for wearing protective equipments was 2.43 ± 0.83. Awareness score was associated with sex, age and family income. Female, older people and people with high income were tend to show low awareness score. Performance score was associated with sex, family income and educational level. Female, people with low education and high income were tend to show low performance score. Conclusion : We concluded that educational effort for noticing the harmful affects of pesticide, the importance of keeping safety guideline and wearing protective equipments to farmers especially female, older people, people with low education and high income were significant factors for prevention of pesticide intoxication.