연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

Euonymus alatus추출물의 실험적 당뇨발생 억제효과

김태중,송희종,소준노,이정호 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

Insulin-dependent diabetes mellitus (IDDM) is majorly caused by an immune system-mediated destruction of the beta cells in the islets of Langerhans. Patients with IDDM face major changes in lifestyle and the possibility of debilitating and life-threatening complications. In Korean traditional medicine, Euonymus alatus (EA) extract has been widely used for preventing and curing ulcer and inflammation. A previous study has shown that EA itself has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to IDDM by an initial protection against diabetes caused by pancreatic inflammation. The present study was undertaken to evaluate the prophylactic effect of EA extract on the diabetogenesis of streptozotocin (STZ) and alloxan (ALX), using animal model, and to explore the possible mechanisms of its antidiabetic activity. Multiple low doses of STZ (45 mg/Kg for 5 days) in ICR mice and single dose of ALX (60 mg/Kg) in SD rats induced high level of hyperglycemia (defined as plasma glucose level > 300 mg/dl) and remarkably diminished body weight. But, animals that received diabetogenic agents in combination withsEA were maintained plasma glucose level as a normal state ( < 150 mg/dl), and appeared in good health and had similar weights to those of normal control. These results strongly indicated that EA prevented and blocked diabetogenesis of chemicals. EA significantly inhibited the nitric oxide- and tumor necrosis factor-production of mouse peritoneal macrophages. However, EA enhanced interleukin 1 production of macrophages at lower concentration but not at higher. EA itself increased phagocytic activity of unstimulated monocytes, but it did completely block that activity of zymosan- or platelet activating factor-stimulated cells. Taken together, this study led to conclusion that EA itself has a significant prophylactic effects against diabetes, and also suggested that its antidiabetic effects might be manifested by the downregulation of inflammatory process in diabetogenesis.

제2형 당뇨병 환자에서 Voglibose와 Acarbose의 비교임상연구

정인경,정재훈,민용기,이명식,이문규,김광원,정윤이,박중열,홍성관,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:아카보스와 보글리보스는 ­glucosidase inhibitors로써 비록 약리학적 작용이나 부작용에 있어서 두 약물간에 차이가 있다는 것은 잘 알려져 있으나 당뇨병 환자를 대상으로 아직 이에 대한 두 약물간에 직접적인 비교에 대해 연구된 바는 없었다. 이에 저자등은 국내 2형 당뇨병 환자에 대해 유효성과 부작용 발현에 대해 두 약제를 비교하고자 무작위법에 의한 위약 대조군의 이중 맹검법 연구를 시행하였다. 방법:시험 약제 투여 4주간의 관찰기를 설정하여 공복혈당의 변화가 30㎎/dL 이하이고, 식후 혈당이 200㎎/dL 이상인 환자로 기타 제외 기준에 해당하지 않은 환자 53명을 대상으로 하여 보글리보스 군(24명)과 아카보스 군(29명)으로 무작위로 나누었다. 치료기간은 총 8주로 하였으며, 4주간 간격으로 혈청학적 검사와 부작용을 분석하여 치료 효과가 부작용을 평가하였다. 결과:1)혈당 변화:보글리보스군은 식후 1시간 혈당이 치료 후 4주, 8주째 의미 있게 감소하였도, 아카보스군은 식후 1시간과 2시간 혈당이 치료 후 4주, 8주째 의미있게 감소하였다. 또한 관찰기 혈당에 대한 치료 4주째 감소량은 아카보스군에서 더 큰 경향을 보였으나, 치료 8주째에는 두 군 간의 강하정도에 의미있는 차이가 없었다(p=0.569). 2)인슐린 치의 변화:보글리스 군은 식후 1시간 인슐린 치가 치료 전에 비해 치료 4주, 8주째 감소하는 경향을 보였고, 공복 인슐린이나 식후 2시간 인슐린치는 치료전 후에 의미 있는 차이가 없었다. 아카보스군에서는 치료 전후로 공복 인슐린, 식후 1시간과 2시간 인슐린치에 의미 있는 차이를 보이지 않았다. 두약제 간에 치료 전과 치료 8주사이의 식후 2시간 인슐린의 감소량이 보글리보스 군에서 의미있게 높았다(p=0.040). 3)당화혈색소:보글리보스 군은 치료 전에 비해 치료후 당화혈색소가 감소하는 경향을 보였고, 아카보스군은 치료 전에 비해 의미있게 감소하였다. 당화혈색소 변화량은 두 군간에 의미 있는 차이는 없었다(p=0.412). 4)지질대사의 변화:중성지방, 콜레스테롤, 고밀도 진단백 콜레스테롤에 대해 두 군간에 의미 있는 차이는 없었다. 5)부작용:소화기계 부작용의 빈도는 치료 4주째 보글리보스 군에서 의미 있게 낮았으나(p=0.028), 치료 8주째 부작용의 빈도는 두 군간에 의미 있는 차이가 없었다(p=0.215). 결론:2형 당뇨병 환자에서 보글리보스와 아카보스의 두 약제의 임상적 유효성과 부작용발현에 대해 비교한 결과 치료 후 8 주 후 혈당강화효과는 두 약제간에 유사한 효과를 보였으나 보글리보스군에서 4주째의 초기 위장관 부작용이 적었다. Background : Acarbose and voglibose are alpha-glucosidase inhibitors. Although different pharmacological effects and adverse abdominal events associated with the two drugs have been reported, no study directly compared acarbose and voglibose in diabetes has been undertaken. To compare the pharmacological effects and gastrointestinal adverse events between two drugs, a randomized, placebo-controlled, double-bind study was performed in type 2 diabetes patients. Methods : The period of study was 12 weeks(observation period: 4 weeks; treatment period: 8 weeks). Fifty-three patients were randomized into two groups(the acarbose group: 24 patients; the voglibose group: 29 patients). The serum glucose, insulin, fructosamine, HbA_1c, cholesterol, triglyceride and the incidence of adverse events were measured. Results : 1) The reduction of glucose from before treatment to 4 weeks after treatment was significantly higher in the acarbose group, but the change before treatment and 8 weeks after treatment in the two groups was similar(p=0.569). 2) The insulin significantly decreased after voglibose treatment(p=0.040). 3) HbAa_1c level tended to decrease in voglibose group, and there was a significant decrease after acarbose treatment. However, the change in HbA_1c level before and after treatment was similar between the two groups(p=0.412). 4) The two drugs did not cause any other changes in the total, HDL-cholesterol and triglyceride. 5) The number of patients with gastrointestinal adverse events was significantly low 4 weeks after voglibose treatment (p=0.049), but the incidence in the two groups was similar after 8 weeks(p=0.215). Conclusions : Acarbose and voglibose significantly improved postprandial hyperglycemia in diabetes. The incidence of gastrointestinal adverse events was low 4 weeks after voglibose treatment(J Kor Diabetes 26:134~145, 2002).

복강경하 대장절제술을 시행 받는 환자에서 라모세트론과 온단세트론외 술 후 오심 및 구토 예방 효과 비교

김효중;어전영;어근무;어정한;엄세훈;조광래;김명훈 인제대학교 2011 仁濟醫學 Vol.32 No.-

Objectives : We evaluated the efficacy of ramosetron and ondansetron for preventing postoperative nausea and vomiting (PONV) in laparoscopic colectomy. Methods and Materials : Eighty patients who received laparoscopic colectomy were randomly divided into two groups: R group (ramosetron 0.1mg PO) and O group (ondansetron 4mg twice IV). Injection or oral medication was administered before the induction of anesthesia in each group. Injection was administered at the end of surgery in O group. General anesthesia was induced using propofol and rocuronium, and maintained with sevoflurane, remifentanil and air (FiO2 0.5), We investigated the incidences of PONV in each group by the Rhodes index of nausea, vomiting and retching (RINVR) at postoperative 6 and 24 hours. Results : The incidence of PONV was not different between group at each time points after surgery (at postoperative 6 hours: 20% in group R, 17.5% in group 0, at postoperative 24 hours; 12.5% in group R, 7.5% in group 0). There was no difference in the severity of PONV, satisfaction, rescue drug usage. Conclusion : Prophylactic therapy with ramosetron is as effective as conventional prophylactic therapy with ondansetron for preventing PONV in general anesthesia for laparoscopic colectomy.

척추 주위근의 단면적과 등척성 요추부신전 근력간의 상관 관계

권정이,이강우,김현숙,김종문,안중모 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.2

고양이 감각 및 운동겉질에서 calbindin D-28k와 parvalbumin에 대한 면역세포화학적연구

김종중,장인엽,정윤영,윤상필,문정석 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

흰쥐의 척수에 분포하는 Substance P 양성 신경섬유에 관한 면역조직화학적 연구

김영준,정윤영,김종중,문정석,정주현 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

An immunohistochemical study was performed using the specific monoclonal antibody against substance P to demonstrate the substance P immunoreactive nerve fibers in the spinal cord of the rats. 1. In the rat spinal cord using immunofluorence method, substance P immunoreactive cell bodies were not found, but their fibers with beaded appearance were observed in all spinal segments. 2. SP immunoreactive nerve fibers were found with the highest concentration especially in the lumbosacral segments. 3. SP immunoreactive nerve fibers could be seen in the Lamina Ⅰ,Ⅱ, X and a great number of substance P immunoreactive nerve fibers appeared in the IML. 4. A smaller number of nerve fibers were observed in the Lamina Ⅸ of ventral region and in the white matter.

경상남도 일부지역 농업인의 농약관련 인지도 및 실행도 결정요인

김정민,전만중,고광욱,유병철,이용환 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Background : The amount of pesticide to be produced and consumed have rapidly growed in Korea and the evidences for pesticide intoxication to human body, natural environment and consumers have evoked. But the studies concerned to pesticide intoxication are inactive. So, we conducted this study about the determinants of awareness and performance for prevention of pesticide intoxication. Methods : The study conduced during the period from July to August 2007 with population consisted of 111 farmers. The toolkit was produced through referencing previous studies. Data analysis was performed with t-test, ANOVA and multiple linear regression analysis using SPSS 14.0K program. Results : The mean awareness score was 3.07 ± 0.75, the mean of total performance score was 2.72 ± 0.69, mean of subtotal performance score for keeping safety guideline was 2.72 ± 0.69 and mean of subtotal performance score for wearing protective equipments was 2.43 ± 0.83. Awareness score was associated with sex, age and family income. Female, older people and people with high income were tend to show low awareness score. Performance score was associated with sex, family income and educational level. Female, people with low education and high income were tend to show low performance score. Conclusion : We concluded that educational effort for noticing the harmful affects of pesticide, the importance of keeping safety guideline and wearing protective equipments to farmers especially female, older people, people with low education and high income were significant factors for prevention of pesticide intoxication.

진공차단기용 영구자석형 엑추에이터 최적설계

김한균,김중경,이정근,한성진 東亞大學校附設 情報技術硏究所 2005 情報技術硏究所論文誌 Vol.13 No.1

The vacuum interrupter operated by permanent magnet actuator(PMA) gives outlook on improved characteristic, higher reliability and cost price reduction as well as the feature of simple structure and few component. For the anlysis and design of permanent magnetic actuator, not only electric, magnetic and mechanical phenomena but also material properties should be taken into account. Aim of the present paper is modeling of permanent magnet actuator(PMA) used in medium voltage distribution systems and remodeling by permanent magnetic. Coupled finite element method is used to analysis the modeling and we compared with previous PMA model and improved PMA model.

유니사이클 로봇에 대한 인간적 추론 제어 메카니즘

김중완,안찬우,전태옥,전언찬,정희균 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

Our unicycle has simple mechanical structure.But unicycle's dynamical system is a very sensitive unstable nonlinear system. equations of motion for a simple unicycle robot were derived using Lagrage's method. In this paper, a human fuzzy influence control mechanism was established throught an inquiry into human riding a unicycle, and we developed a hybrid controller to control our unicycle robot. This proposed hybrid controller is consisted with a pure PD and two fuzzy gain scheduled controllers and a direct fuzzy logic controller. Computer simulation results show that our hybrid controller has robust control performnance to unstable nnonlinear unicycle robot system.