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Optimal Fermentation Conditions for Enhanced GlutathioneProduction by Saccharomyces cerevisiae FF-8
Jae-Young Cha1,,Jin-Chul Park,Beong-Sam Jeon,Young-Choon Lee,Young-Su Cho 한국미생물학회 2004 The journal of microbiology Vol.42 No.1
The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
보문 : 버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 분비 독소(tolaasin)를 저해하는 미생물 Pseudomonas sp. HC1
이찬중 ( Chan Jung Lee ),유영미 ( Young Mi Yoo ),한주연 ( Ju Yeon Han ),전창성 ( Chang Sung Jhune ),정종천 ( Jong Chun Cheong ),문지원 ( Ji Won Moon ),서장선 ( Jang Sun Suh ),한혜수 ( Hye Su Han1 ),차재순 ( Jae Soon Cha ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.4
A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA.. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.
RFID 사용요인이 신뢰, 지각된 위험, 모바일RFID환경 하에서 사용의도에 미치는 영향 -약국RFID 시스템을 중심으로-
최재영 ( Jae Young Choi1 ),차재빈 ( Jae Bin Cha1 ),김상만 ( Sang Man Kim ) 경희대학교 경영연구원 2013 의료경영학연구 Vol.7 No.1
This study examined the influence of various factors of RFID use in mobile RFID environment, focusing on pharmacies` RFID systems. The purpose of the study was to verify the possibility of a more widespread use of RFID and mobile RFID in medical industry by exploring what impact RIFD use factors have on the intention to use in RFID environment. The subjects were pharmacists who have their own pharmacies in Seoul and Gyeonggi Province. Of 400 questionnaires distributed to the respondents, 392 were used in the final analysis except 8 with insufficient answers. The empirical analysis results showed that perceived security, perceived cost, and social influence had significant impacts on trust, while social influence and perceived cost had significant impacts on perceived risk. Among RFID use factors, social influence, perceived security, and perceived risk had significant impacts on intention to use in mobile RFID environment. Trust and perceived cost did not have a significant impact. The summary of analysis results, implications, and limitations are presented in the conclusion.