Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

수정 아인쉬타인 방법에 의한 만경강 유입 유사량 산정

김형산, 맹승진, 정세웅, 이승욱 忠北大學校 農業科學硏究所 2011 農業科學硏究 Vol.27 No.4

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본태성 혈소판 증다증 2예

주기산,임현성,김미자,양태영,허경,김완중,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Essential thrombocythemia is a chronic myeloproliferative disorder of unknown origin, charcterized by excessive number of morphologically and functionally abnormal platelet in peripheral blood and abnormal proliferation of megakaryocytes in bone marrow. Its main clinical manifestations are hemorrhages and thromboses. The aim of therapy is suppression of the excessive bone marrow activity, which can be achieved by radioactive phosphorus, alkylating agent, hydroxyurea and interferon. Two cases were diagnosed at our hospital which had laboratory and clinical features compatible with this disease. We have successfully treated patient with hydroxyurea and platelet antiaggregating agent.

경추 손상후 사망례에 대한 분석

정호성,김상은,이준형,박철완,이 근 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Mortality after cervical spine injury(CSI) is reported as high as 10∼30%, and it is intimately related to the initial management and cardiopulmonary function. We analyzed the cause of death for 29 patients who expired at Chung Ang Gil Hospital after CSI between 1990 and 1994. Of 198 patients with CSI, 43 patients(21m7%) died. Except 14 cases with concomitant fatal head injury and/or multiple systemic injuries, the mortality rate of CSI was 14.6%. Male to female ratio was over 6:1. Fourth to sixth decades constituted 69.0%. Common causes of CSI were traffic accidents(58.6%) and fall/slip(34.5%). The level of CSI was above C5(upper cervical group: UCG) in 65.5%, and below C4(lower cervical group: LCG) in 34.5%. Most of them(86.2%) died within four weeks after CSI. Early death(within 7 days) was more common in the UCG(57.9%) than the LCG(20.0%). Associated injuries were found in 72.4%, and the head injury was the most common one. The higher the Injury Severity Score(ISS), the earlier the patients expired(Fisher`s test, P<0.05). On arrival, hypotension(systolic blood pressure below 90mmHg), bradycardia(less than 60/min), and respiratory arrest was found in 31.0%, 34.5% and 17.2%, respectively. The episode of bradycardia was found in 58.6%. It was more common in cases of early death(69.2%) than those of the late death(50.0%). Hypoxia, hypercarbia, and acidosis was observed in 20.7%, 17.2%, and 51.7%, respectively. Respiratory failure was responsible for the majority(89.7%) of death. In UCG and the cases of the early death, primary respiratory dysfunction was the main cause of death, while in LCG and the cases of the late death, pulmonary complication was the major reason(Fisher`s test P<0.01, P<0.01, respectively).

비글개에서 분만 후 자궁수복의 연속적 초음파상

손형원, 김홍석, 김형진, 김일화, 이기창, 이청산, 강현구 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

A TGF-β-Inducible Cell Adhesion Molecule, βig-h3, Is Downregulated in Melorheostosis and Involved in Osteogenesis

Kim, Jung-Eun,Kim, Eui-Hyun,Han, Eun-Hee,Park, Rang-Woon,Park, Il-Hyung,Jun, Soo-Han,Kim, Jung-Chul,Young, Marian F.,Kim, In-San 경북대학교 병원 2001 경북대학교병원의학연구소논문집 Vol.5 No.1

Melorheostosis is a rare bone disease characterized by linear hyperostosis and asscociated soft tissue adnormalities.The skin overlying the involved bone lesion is often tense, shiny, erythematous,and scleodermatous. In order to look for genes differentially expressed between the normal and involved skin, we cultured skin fibroblasts from the skin lesions of several afflicted patients and identified differentially expressed genes by reverse dot-blot hybridization. We found that the genes human TGF-β-induced gene product(βig-h3),osteoblast-specific factor2, osteonectin, fibronectin, and typeⅠcollagen were all downregulated in the affected skin fibroblast, with βig-h3 the most significantly affected.The expression of βig-h3 was induced by TGF-βin both affected and normal fibroblast.In an effort to determine the mechansim of bone and skin adnormalitied in melorheostosis, we made recombinant βig-h3. Both immobilized and soluble recombinant βig-h3 proteins with or without an RGD motif inhibited bone nodule formation of osteoblasts in vitro. Taken together,our results suggest that altered expression of several adhesion proteins may contribute to the development of hyperostosis and concomitant soft tissue adnormalities of melorheostosis, with βig-h3 in particular playing an important role in osteogenesis.J.cell.biochem.77:169-178,2000.

단기 유출모형에 의한 대청댐 상류 홍수기 유출 분석

Hyung San Kim,Seung Jin Maeng,Woo Seok Jang,Ju Young Son,Man Ha Hwang 위기관리 이론과 실천 2015 Crisisonomy Vol.11 No.2

본 연구에서는 단기 유출모형인 HEC-HMS를 이용하여 대청댐 상류에 위치한 수통 유역의 홍수기 유출을 분석 하였다. 모형의 초기 매개변수는 국내 적용사례가 많은 경험식을 이용하였으며, 유출곡선번호는 선행함수조건 AMC-Ⅲ에 따라 88으로 추정하였다. 모형의 보정과 검증을 위해 2012년부터 2014년까지 호우사상을 대상으로 유량측정을 실시하였으며 이를 바탕으로 수위-유량관계곡선식을 산정하였다. 산정된 수위-유량관계곡선식을 이용하여 2014년 호우사상을 대상으로 매개변수를 보정하고, 2012년과 2013년의 호우사상으로 매개변수 검증을 실시하였다. 보정과 검증을 통해 모의한 홍수기 유출량을 상대오차를 이용하여 실제 유출량과 비교한 결과 5% 이내의 양호한 결과를 나타내었다. 향후 대청댐 상류지역의 홍수기 유출 분석 시 본 연구에서 선정된 최적매개변수를 적용한다면 보다 신뢰도 높은 홍수기 유출량을 모의 할 수 있을 것으로 사료된다. In this study HEC-HMS rainfall-runoff Model was used for flood season discharge analysis at Sutong watershed located at upstream of Daecheong dam. Initial parameters were computed by using empirical formula commonly applied at domestic level. The runoff Curve Number(CN) estimated to be 88 according to AMC-III. Rating curve was estimated on the basis of measured discharge of major rainfall events from 2012 to 2014 for the calibration and validation of the model. Calibration was performed using the estimated rating curve for major rainfall event of 2014 and verification was performed for major rainfall events of 2012 and 2013. The quantitative comparison between observed and simulated runoff through the calibration and verification showed a good result of relative error within 5%. Applying the parameters calibrated in this study, future flood season runoff analysis at Daecheong dam upstream is believed to be able to simulate a more reliable flood runoff.

Self-Assembled Nanoparticles of Bile Acid-Modified Glycol Chitosans and Their Applications for Cancer Therapy

Kim Kwangmeyung,Kim Jong-Ho,Kim Sungwon,Chung Hesson,Choi Kuiwon,Kwon Ick Chan,Park Jae Hyung,Kim Yoo-Shin,Park Rang-Won,Kim In-San,Jeong Seo Young The Polymer Society of Korea 2005 Macromolecular Research Vol.13 No.3

This review explores recent works involving the use of the self-assembled nanoparticles of bile acid-modified glycol chitosans (BGCs) as a new drug carrier for cancer therapy. BGC nanoparticles were produced by chemically grafting different bile acids through the use of l-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). The precise control of the size, structure, and hydrophobicity of the various BGC nanoparticles could be achieved by grafting different amounts of bile acids. The BGC nanoparticles so produced formed nanoparticles ranging in size from 210 to 850 nm in phosphate-buffered saline (PBS, pH=7.4), which exhibited substantially lower critical aggregation concentrations (0.038-0.260 mg/mL) than those of other low-molecular-weight surfactants, indicating that they possess high thermodynamic stability. The SOC nanoparticles could encapsulate small molecular peptides and hydrophobic anticancer drugs with a high loading efficiency and release them in a sustained manner. This review also highlights the biodistribution of the BGC nanoparticles, in order to demonstrate their accumulation in the tumor tissue, by utilizing the enhanced permeability and retention (EPR) effect. The different approaches used to optimize the delivery of drugs to treat cancer are also described in the last section.

충수돌기에 발생한 암육종(Sarcomatoid carcinoma) 1예

( San Kim ),( Hyung Wook Kim ),( Dong Hyuk Cha ),( Woo Sung Jo ),( Tae Ik Park ),( Jong Ho Hwang ),( Dae Hwan Kang ),( Choel Woong Choi ) 대한내과학회 2011 대한내과학회 추계학술발표논문집 Vol.2011 No.1

A sarcomatoid carcinoma or carcinosarcoma is very rare biphasic tumor characterized by a combination of malignant epithelial and mesenchymal cells. Our case was a-51-year-old female, with palpable mass on rt. lower abdominal lesion. At first, we checked her abdominal CT &diagnosed mucocele /c adenocarcinoma on rt. colon. Then, laparoscopic hemicolectomy &lymph node dissection was performed. But histollogically, the tumor was Sarcomatoid carcinoma, arising in mucinous cystadenocarcinoma in the appendix. The mass size was 9.5cm length approximately &infiltrated the appendix. Immunohistochemically, the tumor cell showed a positive immunoreactions for vimentin &pancytokeratin. It means the tumor has two potential cells; epithelial and mesenchymal cell elements. Scarcomatoid carcinoma of colon is malignant tumor with poor prognosis. The patient was treated with doxorubicin &cisplatin therapy, but we lost the patient. A sarcomatoid carcinoma of colon is extremely rare tumor. We found one case &present it with review of the literatures.

Construction and Application of Rainfall-Runoff Model during Low Flow at Youngsan River Watershed

Hyung San Kim(김형산),Seung Jin Maeng(맹승진),Ju Ha Hwang(황주하),Ji Sung Park(박지성) 위기관리 이론과 실천 2017 Crisisonomy Vol.13 No.6

영산강 하구둑은 1981년 준공된 이후 토사유입으로 인한 내용적 감소, 평균 해면 상승 및 기상요인의 변화로 인해 배수갑문 확장에 따른 효율적인 물관리가 필요한 실정이다. 또한 2008년부터 시작된 4대강 살리기 사업의 일환으로 영산강 본류에 승촌보와 죽산보 건설 및 하구둑 구조개선 사업으로 수공구조물이 증가하였으며, 최근 지속적으로 발생하는 가뭄에 효과적으로 대응하기 위해 새로운 물관리 체계의 정립이 필요하게 되었다. 본 연구에서는 영산강유역의 물순환 과정을 모의하기 위해 SSARR 모형을 선정하였으며, 분석에 필요한 물리적 입력변수, 수문기상 입력변수, 내부처리 매개변수, 취수량 및 회귀수량을 산정하였다. 구축된 입력자료와 매개변수를 이용하여 2008년부터 2009년 실측자료로 매개변수 보정을 실시하였으며, 2013년과 2014년 자료를 적용하여 검정을 실시한 결과 3개 제어지점의 이수기 평균 절대오차는 4.7㎥/s, 2014년에는 7.8㎥/s로 분석되었다. 향후 본 연구에서 적용된 물순환 체계를 이용한다면 영산호의 효율적인 물관리를 수행할 수 있을 것이다. Since 1981, Youngsan river bank has become hollow and its water management has become ineffective. Following the project revitalizing the four major rivers in Korea that started in 2008, it is necessary to establish a new water management system due to the increase in the number of water structures. In this study, the SSARR model was selected to simulate the water circulation process at Youngsan river watershed, and the water circulation system for the Youngsan lake was established using the observed input data. The parameters calibration was performed from 2008 to 2009, which was verified with the data from 2013 to 2014. According to the varication with the 2013-14 data, the average absolute error of the last three stations was 4.7㎥/s in 2013 and 7.8㎥/s in 2014, respectively. The water circulation system applied in this study could help carry out an efficient water management of freshwater lake in Korea.