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Hye Jin Choi(최혜진),Bo Young Oh(오보영),Mi Yeon Lee(이미연),Yeon Ja Koh(고연자),Young Woo Gong(공용우),Myung Je Hur(허명제),Jea Mann Lee(이제만),Young Hee Kim(김용희),Hye Sook Jeong(정혜숙),Doo Sung Cheon(천두성) 한국생명과학회 2012 생명과학회지 Vol.22 No.5
인천지역 병·의원에 내원한 급성위장염환자의 대변 검체 총 11,607건을 효소 면역법(ELISA)을 이용하여 group A rotavirus의 계절별 발생 양상을 조사한 결과 2005년부터 2010년간 인천지역에서는 1~2월이 가장 많이 발생하였으나, 기간별로 차이가 존재했다. 또, group A rotavirus 양성 검체 160건에 유전자 분석 결과 VP4는 P8형, VP7는 G1형, G와 P 조합형으로는 G1P8이 가장 많았다. 그러나 이전의 연구와 비교했을 때 연구 지역과 기간에 따라 혈청형과 유전형의 변화가 빈번하였으므로 효율적인 방역을 위해 지속적인 모니터링이 필요하다고 사려된다. 또 이번 연구는 현재 유통 중인 rotavirus 백신의 효율적인 사용의 기초 자료가 될 것이다. Rotavirus is the main cause of severe diarrhea in infants and young children of the world. However, the frequency of genetic alterations makes it hard to control the prophylaxis. Therefore, continuous monitoring of the rotavirus’s genetic change is inevitable to prevent disease prevalence and is useful in inventing an efficient vaccine. From January 2005 to December 2010, we investigated 11,607 stool samples of acute gastroenteritis patients in the Incheon metropolitan area. About 13.18% (1,530 stool samples) of all samples had a positive reaction against rotavirus using an antigen capture enzyme- linked immunosorbent assay (ELISA). Then, the 160 stool samples were searched for subtypes of group A rotavirus by using a reverse transcription polymerase chain reaction (RT-PCR) and a nested multiplex RCR. In P sub-typing, P8 (56.3%) was an extremely prevalent genotype, followed by P6 (21.3%), and P1A (10.0%). G1 (39.4%) was most widespread in the G subtype, followed by G4 (25.0%) and G3 (18.8%). G1P8 (35.5%) was the most common G and P subtype combination, followed by G4P6 (19.3%) and G3P8 (13.1%). These results might be useful data for understanding the epidemiological status of group A-rotavirus dispersion in the Incheon metropolitan area.
Koh, Hye Yeon,Lee, Jun Hyuck,Han, Se Jong,Park, Hyun,Lee, Sung Gu Humana Press 2015 Applied biochemistry and biotechnology Vol.175 No.2
<P>Antifreeze proteins are a group of proteins that allow organisms to survive in subzero environments. These proteins possess thermal hysteresis and ice recrystallization inhibition activities. In the present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Arctic yeast Leucosporidium sp. AY30, LeIBP, in cryopreservation of the marine diatom Phaeodactylum tricornutum, which is one of the classical model diatoms and has most widely been studied with regard to its ecology, physiology, biochemistry, and molecular biology. P. tricornutum cells were frozen by either a fast or two-step freezing method in freezing medium containing 10 % dimethyl sulfoxide, glycerol, propylene glycol, and ethylene glycol, respectively, with or without LeIBP supplement. When cells were frozen using the two-step freezing method, cell survival was significantly increased and statistically the same as that of unfrozen native cells in the presence of 0.1 mg/ml LeIBP in 10 % propylene glycol or 10 % ethylene glycol at day 11 of post-thaw culture. In the presence of LeIBP, the concentration of chlorophyll a was dramatically increased to 14-, 48-, 1.6-, and 8.8-fold when cells were frozen in freezing medium containing dimethyl sulfoxide (DMSO), glycerol, propylene glycol (PG), and ethylene glycol (EG), respectively. Scanning electron microscopy observations demonstrated that the cells were also successfully preserved and epitheca or hypotheca were not deformed. These results demonstrate that LeIBP was successfully applied to improve cryopreservation of the marine diatom P. tricornutum.</P>
( Hye Yun Park ),( Byeong Ho Jeong ),( Won Jung Koh ),( Eun Kyung Kim ),( Seong Yong Lim ),( Ji Hyun Lee ),( Yeon Mok Oh ),( Sang Do Lee ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: Adiponectin and leptin regulate metabolic and inflammatory systems reciprocally. In COPD, leptin was associated with BODE index and adiponectin was proved as a biomarker associated with radiologic-assessed emphysema. However, there are limited data of adiponectin/leptin ratio on longitudinal association of lung function and emphysema. The objective of this study is to investigate the predictable role of adiponectin/leptin ratio on the COPD progression. Methods: Two hundred five COPD subjects in this study were taken from Korean Obstructive Lung Disease (KOLD) cohort. The subjects extracted from the KOLD cohort met all of the following criteria (1) diagnosed with COPD, i.e., postbronchodilator FEV1/FVC < 0.7 and more than 10 pack-years of smoking history as well as no or minimal abnormality on chest radiographs; (2) with follow-up of at least 3 years; (3) with blood sample at enrollment and (4) with pulmonary function test and chest CT scan results during follow-up period. Results: Plasma adiponectin concentration were positively related to initial computed tomography-determined % emphysema (coefficient, 11.699; 95% CI, 2.739 - 20.660; Adjusted P = 0.011), while plasma leptin concentration had a significant inverse correlation with initial FEV1 (adjusted coefficient, -0.187; 95% CI, -0.369 - -0.005; Adjusted P = 0.044). Unexpectedly, increased plasma ratio of adiponectin to leptin was significantly associated with reduced rate of progression of % emphysema over 3 years (adjusted coefficient, -1.464; 95% CI, -2.664 - -0.264; Adjusted P = 0.017), while plasma ratio of adiponectin to leptin had no relationship with FEV1 decline over 3 years. Conclusions: The directionality of association between plasma adiponectin and tomography-determined % emphysema is uncertain, however, the inverse relationship between plasma adiponectin/leptin ratio and change of % emphysema might suggest a protective role of adiponectin to development of emphysema.
( Yeon Sik Hong ),( Hye Min Jeong ),( Ji Young Yoon ),( Eun Jung Park ),( Jiwon Hwang ),( Hyung Jin Kim ),( Joong Kyong Ahn ),( Jae Joon Lee ),( Eun Mi Koh ),( Hoon Suk Cha ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Objectives: To evaluate the clinical characteristics and outcomes of nonradiographic axial spondyloarthritis (nr-axSpA) in Korean patients. Methods: A retrospective analysis evaluated 155 patients with nr-axSpA at a single tertiary hospital between January 2001 and January 2011. Baseline characteristics and clinical courses were reviewed and compared with those of patients with ankylosing spondylitis (AS). Results: The mean age at disease onset was 29. 5 ± 10. 8 years, and 52 (33. 5%) patients were female. The mean age at symptom onset was older (29. 5 ± 10. 8 vs. 25. 9 ± 9. 2, respectively, p < 0. 001) and the male to female ratio was lower (2:1 vs. 5:1, respectively, p = 0. 001) in patients with nr-axSpA compared with patients with AS. The proportion of females was higher among patients with late onset-SpA than early- onset nr-axSpA (55. 0 vs. 30. 1%, respectively, p = 0. 029). Among 74 patients with nr-axSpA, whose follow-up duration was more than 1. 5 years, 29 (39. 2%) patients progressed to AS during the follow-up period. The proportion of females was lower in progressors that that of non-progressors (13. 8 vs. 44. 4%, respectively, p = 0. 010). Presence of syndesmophyte and minimal X-ray changes at baseline were frequently observed in progressors compared with non-progressors (26. 7 vs. 0. 0 %, p = 0. 006 and 69. 0 vs. 35. 6%, p = 0. 005, respectively). Conclusions: The predominance of male patients is more prominent among Korean patients with SpA compared with Caucasians. Female nr-axSpA patients had late symptom onset and less progression to AS. X-ray changes at baseline were associated with radiographic progression.
( Hye Yun Park ),( Byeong Ho Jeong ),( Won Jung Koh ),( Eun Kyung Kim ),( Seong Yong Lim ),( Ji Hyun Lee ),( Yeon Mok Oh ),( Sang Do Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Adiponectin and leptin regulate metabolic and in ammatory systems reciprocally. In COPD, leptin was associated with BODE index and adiponectin was proved as a biomarker associated with radiologic-assessed emphysema. However, there are limited data of adiponectin/leptin ratio on longitudinal association of lung function and emphysema. The objective of this study is to investigate the predictable role of adiponectin/leptin ratio on the COPD progression. Methods: Two hundred five COPD subjects in this study were taken from Korean Obstructive Lung Disease (KOLD) cohort. The subjects extracted from the KOLD cohort met all of the following criteria (1) diagnosed with COPD, i.e., postbronchodilator FEV1/ FVC < 0.7 and more than 10 pack-years of smoking history as well as no or minimal abnormality on chest radiographs; (2) with follow-up of at least 3 years; (3) with blood sample at enrollment and (4) with pulmonary function test and chest CT scan results during follow-up period. Results: Plasma adiponectin concentration were positively related to initial computed tomography-determined % emphysema (coeffi cient, 11.699; 95% CI, 2.739 20.660; Adjusted P = 0.011), while plasma leptin concentration had a significant inverse correlation with initial FEV1 (adjusted coeffi cient, -0.187; 95% CI, -0.369 -0.005; Adjusted P = 0.044). Unexpectedly, increased plasma ratio of adiponectin to leptin was signifi cantly associated with reduced rate of progression of % emphysema over 3 years (adjusted coeffi cient, -1.464; 95% CI, -2.664 -0.264; Adjusted P = 0.017), while plasma ratio of adiponectin to leptin had no relationship with FEV1 decline over 3 years. Conclusions: The directionality of association between plasma adiponectin and tomography- determined % emphysema is uncertain, however, the inverse relationship between plasma adiponectin/leptin ratio and change of % emphysema might suggest a protective role of adiponectin to development of emphysema.
증례 : 내분비-대사 ; 아급성 갑상선염 후 발병된 그레이브스병 1예
서혜미 ( Hye Mi Seo ),범종욱 ( Jong Wook Beom ),김수희 ( Su Hee Kim ),유소연 ( So Yeon You ),김지영 ( Ji Young Kim ),고관표 ( Gwan Pyo Koh ),이상아 ( Sang Ah Lee ) 대한내과학회 2015 대한내과학회지 Vol.89 No.2
Graves` disease following subacute thyroiditis is uncommon. Some patients in these cases showed positive for thyroid antibody only transiently in the resolving phase. However, Graves` disease can rarely be caused by the presence of antibodies after subacute thyroiditis, although the pathophysiology of this is unclear. A 40-year-old woman presented with anterior neck pain and swallowing difficulty. Thyroid function testing showed reduced thyroid-stimulating hormone (TSH) and elevated free thyroxine levels. A thyroid scan revealed decreased uptake in the bilateral thyroid gland. The patient was initially diagnosed with subacute thyroiditis and treated with steroids. Five months later, thyroid function testing showed recurrent hyperthyroidism with positive conversion of TSH receptor antibody, indicating Graves` disease. Since then, she needed the long-term methimazole treatment. In summary, we herein report a case of Graves` disease occurring after subacute thyroiditis. (Korean J Med 2015,89:225-228)
Chitosan을 기질로 하는 설사 치료 천연항생제 개발 2. 말 분변에서 분리된 다제내성 대장균에 대한 철-키토산 유기태화 제재(Fe-OCHT)의 항균효능
노혜지 ( Hye-ji Noh ),김희경 ( Hee Kyong Kim ),송하연 ( Ha-yeon Song ),김준영 ( Joon Young Kim ),김대현 ( Dae Hyun Kim ),조향현 ( Hyang Hyun Cho ),고홍범 ( Hong Bum Koh ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.1
A total 114 E. coli isolates from 73 samples during the period from 2012 to 2013 were obtained. Isolates were confirmed asbeing multidrug resistance (MDR) bacteria isolates showed resistance. Resistance to penicillins was found in penicillin (100%), ampicillin (88%), amoxicillin (89%), oxacillin (100%), ticarcillin (53%), aztreonam (52%), ticarcillin/clavulanic acid (56%) and imipenem (33%) of isolates. The blaTEM genes were detected in 100 (100%) of the 100 ampicillin-resistant isolates. We screened for antimicrobial effect of organic iron mixed chitosan oligosaccharide (Fe-OCHT, Product Name: EPECTROL-3) on multidrug-resistance isolates associated with multidrug resistance in E. coli from feces (73 samples) on mares. We evaluated the antimicrobial activity of organic iron mixed chitosan oligosaccharide against 3 multidrug-resistance E. coli which showed resistance over 15 different antibiotics. The chitosan oligosaccharide (OCHT) inhibited the growth of multidrug resistance E. coli isolates at a concentration of 1 μg/ml in 24 hours. Also, Fe-OCHT showed a significant antibacterial activity against multidrug resistance E. coli isolates at a concentration of 100 μg/ml in 5minutes. Fe-OCHT showed better antibacterial activity than the OCHT. Consequently, we confirmed that Fe-OCHT significantly inhibited multidrug resistance E. coli isolates and might be selected as a more effective natural antibiotics for diarrheal treatment.
이연경 ( Yeon Kyung Lee ),김미경 ( Mi Kyeong Kim ),강혜련 ( Hye Ryun Kang ),김태범 ( Tae Bum Kim ),손성욱 ( Seong Wook Sohn ),박혜경 ( Hye Kyung Park ),고영일 ( Young Il Koh ),장광천 ( Gwang Cheon Jang ),김철우 ( Cheol Woo Kim 대한천식알레르기학회 2015 Allergy Asthma & Respiratory Disease Vol.3 No.3
Purpose: To investigate whether causes of anaphylaxis vary according to regions and seasons in Korean adults. Methods: Based on previous retrospective studies of anaphylaxis between 2007 and 2011 at the 15 university hospitals. Regions were classified into 4 groups: region I, Seoul; region II, Gyeonggi; region III, Chungcheong; and region IV, Chonnam and Busan. The cases induced by 5 major allergens including drugs, food, bee sting, radiocontrast media, and exercise, were analyzed in the present study. Results: Among a total of 1,661 cases reported (53% male, 45.9±16.0 years), 367 (22.2%), 706 (42.5%), 319 (19.2%), and 269 cases (16.2%) were enrolled in regions I, II, III, and IV, respectively. Of the 5 major allergens, drugs (37.3%) were the most frequently reported, followed by food (25.7%), bee sting (17.9%), radiocontrast media (12.9%), and exercise (6.2%). There was no significant difference in the total occurrence of anaphylaxis in 4 seasons (374 in spring, 460 in summer, 460 in autumn, and 367 in winter). Multiple logistic regression analysis revealed that drug-induced anaphylaxis was significantly associated with the winter season (odds ratio [OR] 1.0 vs. OR 0.597, P<0.05 for spring; OR 1.0 vs. OR 0.481, P<0.01 for summer; OR 1.0 vs. OR 0.653, P<0.05 for autumn). Food-associated anaphylaxis was mainly reported in region I, whereas the frequency of insect sting was relatively higher in regions II, III, and IV than in region I. Older age and female gender were significantly associated with drug-induced anaphylaxis. Conclusion: Specific causative allergens of anaphylaxis in adults may vary according to age, gender, region, and season in Korea.(Allergy Asthma Respir Dis 2015;3:187-193)