Pathological Impact on the Phyllosphere Microbiota of Artemisia argyi by Haze

( Yu-zhu Zhang ),( De-yu Jiang ),( Chi Zhang ),( Kun Yang ),( Huai-fu Wang ),( Xiu-wen Xia ),( Wei-jun Ding ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4

The pathological impact of haze upon the phyllosphere microbiota awaits investigation. A moderate degree of haze environment and a clean control were selected in Chengdu, China. Artemisia argyi, a ubiquitously distributed and extensively applied Chinese herb, was also chosen for experiment. Total genome DNA was extracted from leaf samples, and for metagenome sequencing, an Illumina HiSeq 2500 platform was applied. The results showed that the gene numbers of phyllosphere microbiota derived from haze leaves were lower than those of the clean control. The phyllosphere microbiota derived from both haze and clean groups shared the same top ten phyla; the abundances of Proteobacteria, Actinomycetes and Anorthococcuso of the haze group were substantially increased, while Ascomycetes and Basidiomycetes decreased. At the genus level, the abundances of Nocardia, Paracoccus, Marmoricola and Knoelia from haze leaves were markedly increased, while the yeasts were statistically decreased. KEGG retrieval demonstrated that the functional genes were most annotated to metabolism. An interesting find of this work is that the phyllosphere microbiota responsible for the synthesis of primary and secondary metabolites in A. argyi were significantly increased under a haze environment. Relatively enriched genes annotated by eggNOG belong to replication, recombination and repair, and genes classified into the glycoside hydrolase and glycosyltransferase enzymes were significantly increased. In summary, we found that both structure and function of phyllosphere microbiota are globally impacted by haze, while primary and secondary metabolites responsible for haze tolerance were considerably increased. These results suggest an adaptive strategy of plants for tolerating and confronting haze damage.

Experimental Study on Time-dependent Behavior of Silty Mudstone from the Three Gorges Reservoir Area, China

Huai Chang Yu,Han Dong Liu,Zhi Quan Huang,Guang Cheng Shi 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.3

Short-term and creep tests were performed on saturated silty mudstone obtained from the Three Gorges Reservoir area. Based on the triaxial creep test results, the variations of creep strain and creep rate of silty mudstone with time were analyzed in detail, and the differences between the axial and lateral creep parameters were quantitatively studied at different deviatoric stress levels. The longterm strength of silty mudstone was obtained. The results demonstrate that the lateral creep characteristics are more significant than the axial creep characteristics for silty mudstone in the tests. Analysis of the lateral creep characteristics of the rock is a reasonable approach to determine whether creep failure occurs in the silty mudstone. The long-term strength of silty mudstone decreases remarkably, and it is actually the lateral long-term strength of the rock. According to the triaxial creep curves of silty mudstone, a new nonlinear viscoelasto-plastic creep model was proposed by connecting in series the nonlinear viscoplastic body with the sixcomponent model. Comparing the model curves with test results, this model was determined to reflect the creep properties of silty mudstone accurately. Finally, the selection of the creep model parameters was achieved by considering the axial and lateral creep characteristics of silty mudstone.

評李退溪對于王陽明‘知行合一’說的批評

余懷彦(Yu Huai-yan) 한국양명학회 2000 陽明學 Vol.- No.4

Effect of Prior Microstructures on Cementite Dissolution Behavior During Subcritical Annealing of High Carbon Steels

Xiao‑Yu Zhao,Xian‑Ming Zhao,Chun‑Yu Dong,Yang Yang,Huai‑Bin Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

The variation of the morphology and distribution of cementite particles in different prior structures with spheroidizingannealing process has been proceeded in this paper. It is found that the dissolution and coarsening progresses of variousinitial structures in spheroidizing annealing process are quite asynchronous due to the different interlamellar spacing. Thedissolution rate of degenerated pearlite (D-P) with finer interlamellar spacing is faster. The granular cementite in the spheroidizedmicrostructure is fine, uniform and dense. The mean diameter of spherical cementite is refined to 233 nm. However,the initial structure of degenerated pearlite (D-P) is highly sensitive to the austenitization temperature. The cementite iseasy to be coarsened under high austenitizing temperature. The coarsening is accompanied by the gradual increase of theCr content in the cementite, which increases the stability of the cementite. Therefore, the optimal austenitizing temperaturefor degenerated pearlite (D-P) is suggested to be 770 °C.

Numerical simulation of unsteady propeller force for a submarine in straight ahead sailing and steady diving maneuver

Yu-cun Pan,Huai-xin Zhang,Qi-dou Zhou 대한조선학회 2019 International Journal of Naval Architecture and Oc Vol.11 No.2

In order to provide a complementary perspective to the effects of the maneuvering motions on the unsteady propeller performance, the numerical simulation of the flow field of the hull-rudder- propeller system is performed by Unsteady Reynolds-averaged Naiver-Stokes (URANS) method. Firstly, the flow fields around the submarine model without the presence of propeller in straight ahead motion and the steady diving maneuvers with submergence rudder deflections of 4, 8 and 12 are predicted numerically. The non-uniformity characteristic of the nominal wake field is exacerbated with the increase of submergence rudder angle. Then the flow field around the SUBOFF-G submarine fitted with the 4381 propeller is simulated. The axial, transverse and vertical unsteady propeller forces in different maneuvering conditions are compared. In general, as the submarine maneuvers more violently, the harmonic amplitudes of the unsteady force at the 2BPF and 3BPF increased more significantly than that at BPF.

Questioning during Story-telling Activities in Singapore Pre-schools

Huai Le Renee Phee,Nirmala Karuppiah,Yue Yu The Pacific Early Childhood Education Research Ass 2020 Asia-Pacific journal of research in early childhoo Vol.14 No.3

Children attending pre-schools should acquire the necessary knowledge, skills, and dispositions to develop holistically. Therefore, promoting thinking skills in children is important. One of the ways to develop and promote thinking skills in children is through “dialogue such as questioning and challenging” (Higham et al., 2010, p. 393). This study looks into pre-school teachers’ conversations with children during story-telling activities. Six Kindergarten 1 (K1) and Kindergarten 2 (K2) teachers’ story-telling activities with children were audio recorded. These recordings were then analyzed with regard to the number and type of questions asked by the teachers. Teachers also participated in qualitative interviews about their views on story-telling activities. Results have shown that teachers mostly asked factual and procedural questions. The questions asked also belonged to the lowest cognitive level of Bloom’s Taxonomy of learning domains. While most teachers agree questions could help to facilitate children’s thinking, some of them think that questions of the higher cognitive levels may be too demanding for children of this age. These results have future implications for various stakeholders.

The Interaction of Oxytocin and Social Support, Loneliness, and Cortisol Level in Major Depression

Tsung-Yu Tsai,Huai-Hsuan Tseng,Mei Hung Chi,Hui Hua Chang,Cheng-Kuan Wu,Yen Kuang Yang,Po See Chen 대한정신약물학회 2019 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.17 No.4

Objective: Loneliness is a specific risk factor for depressive symptoms and suicidal behavior. The present study examined whether the serum oxytocin level would interact with social support and buffers loneliness and hypothalamic-pituitary- adrenal (HPA)-axis activity in drug-naïve patients with major depressive disorder (MDD). Methods: Twenty-six patients with MDD (male:female = 3:23; mean age, 45.54 ± 12.97 years) were recruited. The 17-item Hamilton Depression Rating Scale, UCLA Loneliness Scale and self-reported Measurement of Support Function Questionnaire were administered. Serum oxytocin and cortisol levels were assessed using a commercial immunoassay kits. Results: In MDD patients, a negative association was found between degrees of social support and loneliness ( = −0.39, p = 0.04). The interaction between social support and serum oxytocin level was negatively associated with loneliness ( = −0.50, p = 0.017) and serum cortisol level ( = −0.55, p = 0.020) after adjusting for age. Follow-up analyses showed that the association between higher social support and lower loneliness was observed only in the higher-oxytocin group (r = −0.75, p = 0.003) but not in the lower group (r = −0.19, p = 0.53). The significance remained after further adjusting for sex and depression severity. Conclusion: Low oxytocin level is a vulnerability factor for the buffering effect of social support for loneliness and aberrant HPA-axis activity in MDD patients.

Property Studies of Optical Waveguide Formed by keV He-Ion Implanted into a Nd:CNGG Crystal

Chunxiao Yu,Jin-Hua Zhao,Huai-Jin Zhang,Xue-Lin Wang 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6

A planar optical waveguide was formed in a neodymium-doped calcium niobium gallium garnet (Nd:CNGG) crystal by using 500-keV He-ion implantation with a dose of 2 × 1016 ions/cm2 at room temperature. The dark modes were observed by using a prism coupling method, and the refractive index profile was reconstructed using a reflectivity calculation method (RCM). The SRIM2006 code (stopping and range of ions in matters, code 2006) was used to simulate the damage profile in the ion-implanted sample. The photoluminescence spectra of the samples showed fairly good potential for used as a laser waveguide.

Numerical simulation of unsteady propeller force for a submarine in straight ahead sailing and steady diving maneuver

Pan, Yu-cun,Zhang, Huai-xin,Zhou, Qi-dou The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.2

In order to provide a complementary perspective to the effects of the maneuvering motions on the unsteady propeller performance, the numerical simulation of the flow field of the hull-rudder- propeller system is performed by Unsteady Reynolds-averaged Naiver-Stokes (URANS) method. Firstly, the flow fields around the submarine model without the presence of propeller in straight ahead motion and the steady diving maneuvers with submergence rudder deflections of 4°, 8° and 12° are predicted numerically. The non-uniformity characteristic of the nominal wake field is exacerbated with the increase submergence rudder angle. Then the flow field around the SUBOFF-G submarine fitted with the 4381 propeller is simulated. The axial, transverse and vertical unsteady propeller forces in different maneuvering conditions are compared. In general, as the submarine maneuvers more violently, the harmonic amplitudes of the unsteady force at the 2BPF and 3BPF increased more significantly than that at BPF.

SSR analysis reveals molecular variation and genetic diversity in Malus sieversii with different stress resistance levels in China

Shaoshuai Yu,Wenxia Zhao,Yanxia Yao,Wenxia Huai,Yuan Cheng,Zhongfu Zhou,Wenfa Xiao,Weiquan Qin,Wei Yan,Weiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.6

Malus sieversii, a valuable crop in Xinjiang, China, is an important natural resource for researching the genetic diversity and phylogenetic evolution of the genus Malus worldwide. Samples from 152 M. sieversii individuals with different symptom grades of deadwood were collected from Gongliu and Xinyuan and analysed with 21 pairs of simple sequence repeat (SSR) primers, revealing molecular genetic characteristics and phylogeny of different groups and individuals. The samples showed high genetic diversity at the species level (Na = 10.76, Ne = 4.37, H = 0.73, I = 1.65, Ho = 0.71, and He = 0.73). Variation was mainly found within groups with lower genetic differentiation (Fst = 0.092) and higher gene flow (Nm = 2.67). A total of 226 alleles were obtained, of which 25 exclusive alleles were from samples with < 40% deadwood rate, and 23 exclusive alleles were from samples with > 40% deadwood rate. Specific bands relating to individuals with deadwood rate < 10% (10 exclusive alleles) or > 60% (7 exclusive alleles) were amplified with 11 pairs of SSR primers. The number of exclusive alleles from M. sieversii plants collected in Gongliu was 52 and from those collected in Xinyuan was 24. Using unweighted pair-group method with arithmetic cluster analysis, the groups with different symptom grades from different sampling sites were shown to be clearly differentiated and formed several discrete subclades. Significantly, the six groups from Gongliu were further classified into two subclusters: Gongliu 1, including three groups with < 40% deadwood rate, and Gongliu 2, including three groups with > 40% deadwood rate.