乳房 腫瘍에 대한 病理學的 硏究

나기준,김수홍,서재홍 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Using an antiserum against cardnoembryonic antigen, which was free from non-specific cross-reacting antigen activity, carcinoembryonic antigen has been demonstrated in 45 out of 90 breast carcinomas by an indirect immunoperoxidase method. The presence of carcinoembryonic antigen appears to be related to good histological type and differentiation, lymph node status and recurrence. Lactoferrin is an iron-binding protein present in saliva, milk, sweat, urine and secretion of the respiratory and digestive systems. These lactofemn might be involved in local and general defense mechanisms against bacterial and proteolytic enzymes. The present immunohistochemical study identifies lysozymes and lactoferrin in breast tumors. The results were as follows : 1) The immunohistochemical localization of carcinoembryonic antigen and lactoferhn was usually confined to intraluminal secretion in fibrocystic disease. 2) Positive staining for cardnoembryonic antigen was confined to intraluminal secretion in fibroadenoma. 3) Positive staining for carcinoembryonic antigen and lactoferrin was confined to intraluminal and epithelial cells in intraductal carcinoma. 4) Positive staining for carcinoembryonic antigen and lactoferrin was confined to intraluminal and epithelial cells in infiltrating ductal carcinoma. 5) Positive staining for carcinoembryonic antigen and lactoferrin was confined to intraluminal and epithelial cells in ductal carcinoma adjacent to normal glands. 6) On the ultrastructural study of breast tumor, Infiltrating ductal carcinoma show loss of basal lamina cell junctions and villi of cytoplasms, In conclusion, carcinoembryonic antigen and lactof en-in were also found in intraductal and ductal carcinoma of breasts. The correlation between the presence of

동해로 유입되는 가곡천과 마읍천에 서식하는 담수어의 지리적 분포

남명모,강영훈,채병수,양홍준 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.4

1999년 4월부터 2000년 10월까지 강원도 삼척시에 위치한 가곡천과 마읍천에서 어류의 지리적분포와 군집구조를 조사하였다. 가곡천에서는 10과 20종, 마읍천에서는 10과 25종의 어류가 확인되었으며, 이 가운데 한국 고유종은 Squalidus multimaculatus, Iksookimia koreensis 그리고 Liobagrus mediadiposalis의 3종이었다. 가곡천의 미기록종은 S. multimaculatus, I. koreensis, Pungitius sinensis sinensis 그리고 Oncorhynchus masou masou이었으며, Zacco platypus와 Z. temmincki가 우점종이었다. 가곡천 상류역에서는 Z. temmincki와 Moroco oxycephalus가 우점종이었으며, 중류역에서는 Z. platypus, 하류역에서는 Cottus Hngiongensis, Chaenogobius urotaenia 그리고 Rhinogobius brunneus가 우점종이었다. 마읍천의 미기록종은 Cyprinus carpio, Pseudorasbora parva, Silurus asotus, O. m. masou, M. oxycephalus, Gasterosteus aculeatus, P. s. sinensis, C. castaneus, Acanthogobius lactipes, Tridentiger brevispines이었으며, Z. platypus가 우점종이었다. 마읍천 상류역에서는 Z. platypus가 우점종이었으며, 중류역에서는 Ladislavia taczanowskii, 하류역에서는 P. s. sinensis가 우점종이었다. 본 조사에서 확인된 어류 가운데, 특히 가곡천에 서식하는 S. multimaculatus와 마곡천에 서식하는 L. taczanowskii와 Nemacheilus toni는 지리분포상 보호되어야하겠다. 가곡천의 종다양성지수는 1.109, 마읍천의 종다양성지수는 1.109로서 종다양성이 높고 군집이 안정되어 있었다. The geographical distribution and community structure of freshwater fishes in the Gagog and Maeup Streams, which flow into the East Sea, were surveyed from April 1999 to October 2000. Twenty species belonging to 10 families were collected in Gagog Stream. Twenty-five species belonging to 10 families were collected in Maeup Stream, including the Korean endemic species Squalidus multimaculatus, Iksookimia koreensis and Liobagrus mediadiposalis. In the Gagog Stream, S. multimaculatus, I. koreensis, Pungitius sinensis sinensis and Oncorhynchus masou masou were newly recorded. The dominant species were Zacco platypus and Z. temmincki. A tendency was seen in regional distribution such that Z. temmincki and Moroco oxycephalus dominated in the upper stream, Z. platypus in the middle stream, and Cottus hangiongensis, Chaenogobius urotaenia and Rhinogobius brunneus in the lower stream. Newly recorded from the Maeup Stream are Cyprinus carpio, Pseudorasbora parva, Silurus asotus, O.m.masou, M. oxycephalus, Gasterosteus aculeatus, P. s. sinensis, C. castaneus, Acanthogobius lactipes and Tridentiger brevispinis. The dominant species was Z. platypus. A tendency was seen in regional distribution such that Z. platypus dominated in the upper stream, Ladislavia taczanowskii in the midddle stream, and P. s. sinensis in the lower stream. Among the noteworthy fishes found in the present study were S. multimaculatus in the Gagog Stream and L. taczanowskii and Nemacheilus toni in the Maeup Stream, These species are considered as species of conservation concern. The species diversity index of the communities were 1.109 in the Gagog Stream and 1.166 in the Maeup Stream. The results indicate that the community structure in each stream is very diverse but stable in its species composition.

Niwaella multifasciata Wakiya et Mori (Cobitidae)의 附屬骨格

成娟濟,楊洪準,南明模,蔡秉洙 경북대학교 과학교육연구소 1988 科學敎育硏究誌 Vol.12 No.-

The appendicular skeleton of Niwaella multifasciata was described as a part of systematic study of Cobitidae. In the pectoral girdle, posttemporal was sigmoid form, and upper and lower arms of cleithrum had an angle of 105°, the length of lower arm was one-third of upper one. The scapular had a foramen in the center, and actionst was consisted in three pairs of bone. Postcleithrum was absent. Pelvic girdle was consisted in a pair of innominatium and three pairs of basiost. In the pterygiophore, dorsal fin was supported by three interneural spine and thirteen basiost, and interneural spine was located between the neura니 spin of the 27-31th vertebrae. Anal fin was supported by five interhemal spine and nine basiost, interneural spine was located between the hemal spine of the 42-45th vertebras.

상피종양의 keratin 생성능에 대한 전자현미경적 연구

이길선,이명주,장정수,이민전,노광을,서재홍 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Keratins are characteristically present in all keratinizing and nonkeratinizing epithelial cells and their neoplasms. They consist of at least 19 different polypeptides ranging from 40 to 69 kilodaltons, which are developmentally related to specific epithelial cell types. Keratins of high and intermediate molecular weights are readily demonstrated in squamous cell carcinomas, which are characteristically rich in tonofilaments and tonofilament-desmosome complexes. Keratins of low molecular weights are expressed in adenocarcinoma including renal cell carinoma, hepatocellular carcinoma, small cell carcinoma, and carcinoid tumor. These tumors do not reveal any tonofilaments and sacrcely show discrete filaments on electron microscopy. An immunoperoxidase technique employing antibody to keratin was used to study distribution and staining pattern of keratin filaments in benign and malignant epithelial tumors (20 squamous cell carcinomas, 20 gastric adenocarcinomas, 20 transitional cell carcinomas and 15 non-neoplastic epithelia). These immunohistochemical results were compared with ultrastructural features of neoplastic diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. The results are as follows : 1. The squamous epithelium, glandular epithelium and transitional epithelium disclosed the positive reaction for keratin. 2. Squamous cell carcinoma and transitional cell carcinoma exhibited positive reaction for keratin. 3. Gastric adenocarcinoma showed uniformly negative or weakly positive reaction for keratin. 4. On electron microscopy, heavy bundles of tonofilaments and desmosomes were more frequently encountered in well differentiated squamous cell carcinoma, In poorly differentiated squamous cell carcinoma, a few tonofibrils and desmosome were noted. 5. In the adenocarcinomas, the intermediate filaments were arranged randomly as nonaggregated, short filaments spread throughout the cytoplasm, although occasionally they formed a perinuclear whorl. These filaments did not become aggregated to form tonofilaments. 6. On the immunogold labeling for keratin of low molecular weight areas in gastric adenocinoma, in tense labeling of intermediate filaments for keratin was noted. From the above result, ultrastructural and immunohistochemical study on malignant epithelial cell were useful in differential diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. It was also proved that immunogold labeling technique was especially valuable for diagnosis of vague case which showed negative or weakly positive reaction in immunohistochemical stain.

간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

경식도 심초음파도의 Direct Planimetry를 이용하여 측정한 대동맥판 면적의 심박출량 및 좌심실 구출율의 변화에 따른 정확도

진승원,김종진,김희열,박지원,전두수,윤호중,박준철,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Agonist-induced internalization of mGluR1&agr; is mediated by caveolin

Hong, Yun Hwa,Kim, Ji Young,Lee, Jeong Ho,Chae, Hong Gu,Jang, Sung Soo,Jeon, Ju Hong,Kim, Chul Hoon,Kim, Jun,Kim, Sang Jeong Blackwell Publishing Ltd 2009 Journal of Neurochemistry Vol.111 No.1

<P>Agonist-induced internalization of metabotropic glutamate receptors (mGluRs) plays an important role in neuronal signaling. Although internalization of mGluRs has been reported to be mediated by clathrin-dependent pathway, studies describing clathrin-independent pathways are emerging. Here, we report that agonist-induced internalization of mGluR1 alpha is mediated by caveolin. We show that two caveolin-binding motifs of mGluR1 alpha interact with caveolin1/2. Using cell surface-immunoprecipitation and total internal reflection fluorescence imaging, we found that agonist-induced internalization of mGluR1 alpha is regulated by caveolin-binding motifs of the receptor in heterologous cells. Moreover, in the cerebellum, group I mGluR agonist dihydroxyphenylglycol increased the interaction of phosphorylated caveolin with mGluR1 alpha. This interaction was blocked by methyl-beta-cyclodextrin, known to disrupt caveolin/caveolae-dependent signaling by cholesterol depletion. Methyl-beta-cyclodextrin also blocked the agonist-induced internalization of mGluR1 alpha. Thus, these findings represent the evidence for agonist-induced internalization of mGluR1 alpha via caveolin and suggest that caveolin might play a role in synaptic metaplasticity by regulating internalization of mGluR1 alpha in the cerebellum.</P>

Photoluminescence and electrical properties of epitaxial Al-doped ZnO transparent conducting thin films

Noh, Jun Hong,Cho, In-Sun,Lee, Sangwook,Cho, Chin Moo,Han, Hyun Soo,An, Jae-Sul,Kwak, Chae Hyun,Kim, Jin Yong,Jung, Hyun Suk,Lee, Jung-Kun,Hong, Kug Sun WILEY-VCH Verlag 2009 Physica status solidi. PSS. A, Applications and ma Vol.206 No.9

<P>Epitaxial ZnO and Al-doped ZnO (AZO) thin films were grown on (0001)-sapphire substrates using pulsed laser deposition. The photoluminescence spectrum of the highly conductive (1.3 × 10<SUP>3</SUP> S cm<SUP>−1</SUP>), as-grown AZO shows a poor near band edge (NBE) emission (3.30 eV) and no deep level emission at room temperature. In addition, the peak (3.386 eV) for the free excitons of AZO showed thermal quenching behavior with two activation energies (38.2 and 10.0 meV). The poor NBE emission is attributed to the nonradiative recombination center created by Al doping. Highly conductive (6.0 × 10<SUP>2</SUP> S cm<SUP>−1</SUP>) and intense NBE emitting AZO films could be achieved by the reduction of the nonradiative recombination centers through hydrogen annealing.</P>

Survey on major pest incidence of rice in 2018

Sung-Jun Hong,Kyong-Jae Lee,Ui-Seok Chae,Hyeong-Il Roh,Jun-yong Chung 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04

전국 136개 시군에 있는 690개소 벼 관찰포에서 12종의 주요 해충을 6월 1일부터 9월 16일까지 15일 간격으로 총 8회 조사한 결과를 국가농작물병해충관리시스템(NCPMS)을 통하여 수집하였다. 2018년도 벼 주요해충 12종의 총 발생 추정면적은 94,049ha로 재배면적 대비 12.7%에 발생되었다. 발생면적이 가장 많았던 해충은 먹노린재로 해충 총 발생면적의 34.9%인 32,779ha(재배면적 대비 4.4%)에서 발생이 확인되었다. 2018년 먹노린재 발생은 평년의 346%, 전년의 155% 수준으로 최근 몇 년간 계속해서 발생이 증가되고 있는 추세이다. 특히 전남(15,191ha)과 충남 (10,041ha) 지역에서 발생이 많은 것을 확인할 수 있었다. 먹노린재 다음으로는 벼물바구미 17,711ha(재배면적 대비 2.4%), 흰등멸구 14,885ha(재배면적 대비 2.0%), 혹명나방 9,130ha(재배면적 대비 1.2%), 애멸구 4,960ha(재배면적 대비 0.7%) 순으로 발생이 많았다. 10년간 평균간 비교하여 발생이 증가된 해충은 먹노린재 1종이었으며 나머지 11종은 발생이 감소된 것으로 확인되었다. 결론적으로 2018년 벼 주요해충의 발생정도는 전년대비 59%, 10년간 평균 대비 38% 수준으로 발생이 낮은 경향임을 알 수 있었다.