Critical role of bile acid (BA) in the cellular entry and permissiveness of Hepatitis B virus in vitro

Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.

연령증가에 따른 혈압요소들의 좌심실비대 및 관동맥질환과의 관련성

박재석,박창규,박미영,박재형,김용현,나진오,신성희,서순용,홍순준,박성미,임홍의,김응주,서홍석,오동주,노영무 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

장거리 해저 광전송망 분산보상 설계 및 구현

유재덕(Jae Duck Yoo),박홍태(Hong Tae Park),신현식(Hyun Sik Shin),김천석(Chun Suk Kim),신윤호(Yun Ho Shin) 한국전자통신학회 2006 한국전자통신학회 논문지 Vol.1 No.1

현재 국내.외 기간 통신망은 급격히 증가하는 고속 대용량 트래픽을 효율적으로 멀리 전송하기 위해 광증폭기를 이용한 파장분할다중화방식 (DWDM:Dense Wavelength Division Multiplexing) 광전송 시스템 통신망을 구축하고 있다. DWDM광전송 시스템에서 고속, 대용량 및 장거리 전송을 하기 위해서는 기존 3R 광 중계기시스템에서 크게 중요시 않았던 광 fiber의 비선형(non-linearity) 전송제약 요소를 고려하여 통신망을 설계하여야 한다. 광통신망에서 장거리 전송 시 신호펄스 파형의 왜곡을 최소화하기 위해 영분산(zero-dispersion)을 구현하여야 하지만, 영분산으로 인하여 파이버의 비선형 현상이 나타나 신호 스펙트럼 왜곡 현상이 발생하는 문제가 있다. 즉 영 분산과 파이버의 비선형 사이에 서로 상충관계를 해결하여야 고속 대용량 트랙픽을 장거리 전송할 수 있다. 본 논문에서는 DWDM 해저 광 통신망에서 고속 대용량 트래픽을 장거리 전송하기 위한 기술을 알아보고, 영분산과 파이버의 비선형 사이에 상충문제를 해결 위한 분산관리 기법을 소개하고, 실제한국-홍콩간 해저 광통신 구간에서 분산 맵 (dispersion map)을 설계 구현하였다. Currently, Most of the Backhual and Submarine s networks are being installed using DWDM and EDFA to deal with dramatically increasing internet traffic. In a conventional type optical system, the so-called 3R repeater, fiber non- linearity was not significant factor but it is one of the most important consideration when designing new DWDM network based on the EDFAs. To minimize the signal pulse distortion in long-haul optical network, Zero-dispersion fiber is indispensable but causes the fiber non-linearity. Therefore this conflicting problems should be managed to transmit a high-speed capacity traffic. In this paper, key technologies in DWDM submarine optic network were found out for a high capacity traffic and introduced the dispersion management way for solving the conflicting problems between Zero-dispersion and non-linearity. Lastly designed a dispersion map in section between Korea and Hong-kong.

수도권 지역의 연령에 따른 전통 떡류의 이용현황

신민자,김옥선,정재홍 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6

The purpose of this study was to investigate the consumption patterns of the Korean traditional rice cakes by general public. Self-administered questionnaires were collected from 511 residents in the Metropolitan area. Data were analysed by t-test, ANOVA and Chi-square. The result of the study can be summarized as follows: Generally, the rice cake was recognized as a "good food" in all age groups. The reasons why they prefer the rice cakes were "It tastes good" and "It has been eaten from the past." The time when they use the rice cake was mostly the holidays and events(or festivities). The female had more experiences than the male in making it. Songpyeon took the first place for them to have experiences to make. The consumption frequencies of rice cake were the more in females than in males. The higher the age, the more frequently they consumed. Injeolmi was the one which was purchased the most frequently. The place that they usually buy from was the rice cake shop around their house. The most important factor considering when they buy it was the taste. The reason why the frequency the rice cakes made for their direct consumption at home is gradually decreasing was "It is troublesome to make", and "It is complicated to make.

텐돈의 긴장력 손실에 따른 프리스트레스 콘크리트 격납건물의 거동해석

진재철,전영선,이홍표 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

The prestressing force of tendon can be decreased by creep of concrete, shrinkage of concrete and relaxation of steel. In this paper, we describe some primary causes of time-dependent losses of prestressing tendon force. New stress reduction model is proposed to predicit time-dependent losses of prestressing tendon force in accurate way. It should be noted that the proposed model is based on the data, which are accumulated from the in-service inspection of posttensioned cotainment building system. Using the new stress reduction model, we carry out finite element analysis to investigate how the losses of prestressing force effect to the structural behaviour of containment building. From FE analysis with new stress reduction model, concrete creep appears as the primary factor which can influence the reduction of prestressing tendon force. It also appears that the relaxation of steel as the secondary influencing factor and shrinkage of concrete as the tertiary factor.

표백제에 따른 외인성 착색치아의 표백효과 및 표면변화에 관한 연구

홍찬의,신동훈,이재윤 大韓齒科保存學會 1995 Restorative Dentistry & Endodontics Vol.20 No.2

입원한 입시 스트레스 증후군 환자의임상양상에 관한 정신의학적 연구

신광철,진태원,이영식,이재우,이재광,이길홍 중앙대부설한국의과학연구소 1991 한국의과학 Vol.23 No.3

실용적인 전자투표 프로토콜 제안

홍영기,이재신,김순석,김성권 중앙대학교 정보통신연구소 1999 정보통신연구소논문집 Vol.1 No.1

정보화 시대에 세계적으로 컴퓨터의 보급 및 네트워크의 발달로 컴퓨터 응용분야에서 많은 발전이 이루어지고 있다. 그 중에서도 암호학을 이용한 전자투표는 전자화 되어 가는 민주 사회에서 필요한 요소로 부각되고 있다. 그러나 지금까지의 전자 투표는 중요성에도 불구하고 많은 문제점이 지적되었다. 본 논문에서는 추적할 수 없는 통신망[1]과 신뢰할 수 있는 선거관리센터를 가정함으로써 전자 투표의 요구사항인 안전성, 공정성, 투표자의 비밀성, 선거 집계의 정확성 등을 만족하는 대규모적이고 실용적인 전자투표 프로토콜을 제안한다. 블라인딩기법(Blinding Technique)을 이용하여 투표자의 투표내용을 노출시키지 않고 투표권을 획득하며, 부정할 수 없는 도전 및 응답프로토콜(Challenge and Responsible Protocol)을 사용하여 투표자와 집계자 사이의 부정을 확인할 수 있으며, 투표자나 집계자의 부정으로 인한 전자 투표의 무결성과 투표자의 프라이버시를 보호하기 위해 투표자의 익명을 기반으로 안전한 전자투표를 방식을 제안한다. We have seen a lot of developments on computer application areas with the wide spread use of computers and the rapid growth of communication network. It is necessary to use a cryptographic technique for electronic voting, but, at present, despite of its importance electronic voting protocols so far have many shortcomings. In this paper, with the assumption of untraceable communication channels and trustable voting centers we propose a large-scale and practical electronic voting protocol satisfying protocol requirements, such as secureness, fairness, privacy of voter and correctness. Voters are able to get a vote without revealing their voted information by using the blinding technique. We can find the injustice between a voter and the tallier by using undeniable challenge and responsible protocol. Also, we proposes a secure protocol that compensates a integrity of electronic voting and protects a privacy of voter from outer attacks as using a anonymity of voter.

전계발광소자용 유기물 초박막의 상거동과 피막특성

신길재,박상권,안택,심홍구 동국대학교 산업기술연구원 2000 산업기술논문집 Vol.11 No.1

최근, 여러 가지 형태의 디스플레이 중 고분자 전계발광 디스플레이(ELD)는 여러 가지 장점 때문에 차세대 디스플레이로서 기대를 모으고 있다. 본 연구에서는 ELD용 적색발광체로서 사용가능한 poly(2-methoxy-5-(2.-ethylhexyloxy)-1,4-phenylene vinylene (MEH-PPV)을 합성하고, MEH-PPV와 전하수송층 단분자막을 공기/물 계면에 형성시켰다. 이 Langmuir 단분자막의 상거동과 EL 소자 양극으로 쓰이는 indium-tin oxide (ITO) glass 상으로의 피막특성을 조사하였다. Recently, polymer electroluminescence display (ELD) of various display techniques has drawn much attention due to its advantages such as fast response, low operation voltage and easy fabrication of huge size device. In this study, we synthesized a luminescence material for polymer ELD, poly(2-methoxy-5-(2-ethylhexyloxy)-1,4 -phenylene vinylene (MEH-PPV). The Langmuir monolayers of charge transport layers as well as the MEH-PPV were formed at the air/water interface. We investigated the phase behaviors and the deposition characteristics into indium=tin oxide substrate of the Langmuir monolayers.

문자 교육에 대한 유치원과 초등학교 교사의 인식 조사

신동로,고재희,홍성근 全北大學校 敎育大學院 1997 敎育論叢 Vol.17 No.-

The purpose of the study was to investigate the perceptions of teachers in preschools and primary schools, in order to research the sequence of literacy curriculum. The results of study were as follows : ⑴ there was a lot of gap of teachers' perceptions between private and public preschools, as well as primary schools. ⑵ most of primary teachers recognized young children's literacy lower than preschool teachers, and ⑶ most of primary teachers, as well as preschool teachers, wanted to adapt formal instruction of literacy in preschools, although they responsed the negative effect of that method. The conclusions of study were as follows : ⑴ literacy curriculum in preschool is reviewed to prescripe the level explicitly, ⑵ preschool education is taken the resposibility on Nation Insitute, in order to reduce the gap of level between private and public preschools, and ⑶ in order to reduce the gap of the perception of teachers between preschool's and primary school's curriculum, it is reformed that the teacher's qualification of the first grade in primary school intergrates that of preschool and inservice education need to be taken together.