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Jung, Haeun,Bae, Seri,Jang, Ha-Lim,Joo, Jung Min Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.10
A palladium-catalyzed C-H arylation reaction of nitroimidazoles and nitropyrazoles was developed using aryl bromides as arene donors. The electron-withdrawing effect of the nitro group allows for direct C-H arylation reactions of the nitro diazoles with high regioselectivity under mild conditions. The new C-H arylation approach is thus complementary to nucleophilic substitution reactions, enabling the preparation of complex nitroazole compounds.
( Kyung Ock Park ),( Haeun Kim ),( Hyejin Kim ),( Eun Hee Lee ),( Hyun Ju Jung ),( Jung Il Choi ),( Jungheon Yoo ),( Sung Hoon Kwon ),( Sung Weon Ryoo ),( Yong Gyun Jung ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Background: Early diagnosis of TB is crucial for both clinically and epidemiologically. Sputum culture is the gold standard for diagnosis of Mycobacterium tuberculosis tuberculosis (TB). The MAC system immobilizes bacteria by using agarose in a microfi uidic culture chamber so that single cell growth can be tracked by microscopy. This study using DAC system for detection of M. tuberculosis isolates from sputum samples. Methods: 74 sputa samples (25 clinical sputa, 49 H37Rv spiking sputa) were included for the diagnosis of TB. All samples were decontaminated using the 4% N-acetyl-Lcysteine (NALC)-NaOH method. Results: In 25 clinical sputa samples, positive rate for Middlebrook 7H11 medium, Ogawa`s medium, and DAC system were determined as 1 (4.0%), 1 (4.0%), and 3 (12.0%), respectively. Negative rate for Middlebrook 7H11 medium, Ogawa`s medium, and DAC system were determined as 23 (92.0%), 23 (92.0%), and 21 (84.0%), respectively. And 1 (4.0%) contamination was observed in all culture methods. In 49 H37Rv spiking sputa samples, time to detection (TTD) was 5 to 13 days in MGIT 960 and 5 to 9 days in DAC system. In a total of 49 H37Rv spiking sputa samples, TTD according to cfu in Middlebrook 7H11 medium was 7 days (5-6 log10 cfu/ml) to 9 days (2-3 log10 cfu/ml) in DAC system. TTD according to cfu in Middlebrook 7H11 medium was 6 days (5-6 log10 cfu/ml) to 12 days (2-3 log10 cfu/ml) in MGIT 960. Conclusions: The DAC system detected the growth of M. tuberculosis more sensitivity than Middlebrook 7H11 and Ogawa`s media. Also, required time for true negative reports in less than 3 weeks. We concluded the DAC culture system is advantageous in terms of turnaround time.
( Kyung Ock Park ),( Haeun Kim ),( Hyejin Kim ),( Eun Hee Lee ),( Hyun Ju Jung ),( Jung Il Choi ),( Jung Heon Yoo ),( Sung Hoon Kwon ),( Sung Weon Ryoo ),( Yong Gyun Jung ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: Early diagnosis of TB is crucial for both clinically and epidemiologically. Sputum culture is the gold standard for diagnosis of Mycobacterium tuberculosis tuberculosis (TB). The MAC system immobilizes bacteria by using agarose in a microfluidic culture chamber so that single cell growth can be tracked by microscopy. This study using DAC system for detection of M. tuberculosis isolates from sputum samples. Methods: 74 sputa samples (25 clinical sputa, 49 H37Rv spiking sputa) were included for the diagnosis of TB. All samples were decontaminated using the 4% N-acetyl-L-cysteine (NALC)-NaOH method. Results: In 25 clinical sputa samples, positive rate for Middlebrook 7H11 medium, Ogawa’s medium, and DAC system were determined as 1 (4.0%), 1 (4.0%), and 3 (12.0%), respectively. Negative rate for Middlebrook 7H11 medium, Ogawa’s medium, and DAC system were determined as 23 (92.0%), 23 (92.0%), and 21 (84.0%), respectively. And 1 (4.0%) contamination was observed in all culture methods. In 49 H37Rv spiking sputa samples, time to detection (TTD) was 5 to 13 days in MGIT 960 and 5 to 9 days in DAC system. In a total of 49 H37Rv spiking sputa samples, TTD according to cfu in Middlebrook 7H11 medium was 7 days (5-6 log10 cfu/ml) to 9 days (2-3 log10 cfu/ml) in DAC system. TTD according to cfu in Middlebrook 7H11 medium was 6 days (5-6 log10 cfu/ml) to 12 days (2-3 log10 cfu/ml) in MGIT 960. Conclusions: The DAC system detected the growth of M. tuberculosis more sensitivity than Middlebrook 7H11 and Ogawa’s media. Also, required time for true negative reports in less than 3 weeks. We concluded the DAC culture system is advantageous in terms of turnaround time.
Choi, Jungil,Yoo, Jungheon,Kim, Ki-jung,Kim, Eun-Geun,Park, Kyung Ock,Kim, Hyejin,Kim, Haeun,Jung, Hyunju,Kim, Taeyoung,Choi, Myungjin,Kim, Hee Chan,Ryoo, Sungweon,Jung, Yong-Gyun,Kwon, Sunghoon Springer-Verlag 2016 Applied microbiology and biotechnology Vol.100 No.5
<P>Tuberculosis (TB) is a major global health problem, and multi-drug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) are spreading throughout the world. However, conventional drug susceptibility test (DST) methods, which rely on the detection of the colony formation on a solid medium, require 1-2 months to the result. A rapid and accurate DST is necessary to identify patients with drug-resistant TB and treat them with appropriate drugs. Here, we used microscopic imaging of Mycobacterium tuberculosis (MTB) immobilized in an agarose matrix for a rapid DST. The agarose matrix, which was molded in a microfluidic chip, was inoculated with MTB, and TB drugs in liquid culture medium diffused throughout the agarose to reach the MTB immobilized in the agarose matrix. After the responses of MTB to drugs were tracked with an automated microscopic system, an image-processing program automatically determined the susceptibility and resistance of MTB to specific doses of TB drugs. The automatic DST system was able to assess the drug susceptibility of various drug-resistant clinical TB strains within 9 days with an accuracy comparable to that of conventional method. Our rapid DST method based on microscopic time-lapse imaging greatly reduces the time required for a DST and can be used to rapidly and accurately treat TB patients.</P>
( Eun Hee Lee ),( Hye Jin Kim ),( Hyun Ju Jung ),( Haeun Kim ),( Jung Il Choi ),( Jung Heon Yoo ),( Kyung Ock Park ),( Yong Gyun Jung ),( Sung Weon Ryoo ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Background: M. tuberculosis culture is major technique for diagnosis of tuberculosis. Owing to the slow growth of M. tuberculosis, development of optimized culture media to enhance Mycobacteria growth rate have been required. Our preliminary data suggested that supplements originated BCG may be suffi cient to improve growth of Mycobacteria. Methods: BCG-Tokyo strains, widely administrated BCG Vaccines, were used as sources to develop the growth promoting supplement. Two species of supplement were prepared in a 4L culture of BCG-Tokyo strains with sauton media. To harvest autolysis portion, BCG-Tokyo extracts were allowed to proceed at 52℃ for 72 hrs in a rotary shakers at 140 rpm with pH 5.4 deionized water contained tween 80. We examined the growth promotion test with different Mycobacterial species, M. tuberculsosis H37Rv and M. Avium. Supplements were evaluated growth rate on the 7H9 broth media and 7H11 agar plate cultures. Results: The enhancing effect of supplements to stimulate culture of M. tuberculosis was represented by liquid medium and solid agar culture systems. Both of supplements showed signifi cant growth promoting effect in the 96well plate with 7H9 broth media calculated at OD 600. As the mycobacteria were plated on the supplements contained 7H11 agar plate, the observed colonies diameter showed larger than control plate. Moreover, when we tested various mixed conditions with routine used growth supplement such as OADC, ADC-tween80 0.5% and FBS, the supplements were represented the effects of advanced TB growth both broth and agar culture systems. Conclusions: According to our results, supplements originated BCG-Tokyo extracts showed an enhancing effect of bacterial growth. These culture supplements should be able to reduce the detection time for diagnosis of Mycobacterium tuberculosis infection.
Links between Serine Biosynthesis Pathway and Epigenetics in Cancer Metabolism
( Haeun Kim ),( Yoon Jung Park ) 한국임상영양학회 2018 Clinical Nutrition Research Vol.7 No.3
Cancer metabolism is considered as one of major cancer hallmarks. It is important to understand cancer-specific metabolic changes and its impact on cancer biology to identify therapeutic potentials. Among cancer-specific metabolic changes, a role of serine metabolism has been discovered in various cancer types. Upregulation of serine synthesis pathway (SSP) supports cell proliferation and metastasis. The change of serine metabolism is, in part, mediated by epigenetic modifiers, such as Euchromatic histone-lysine N-methyltransferase 2 and Lysine Demethylase 4C. On the other hand, SSP also influences epigenetic landscape such as methylation status of nucleic acids and histone proteins via affecting S-adenosyl methionine production. In the review, we highlight recent evidences on interactions between SSP and epigenetic regulation in cancer. It may provide an insight on roles and regulation of SSP in cancer metabolism and the potential of serine metabolism for cancer therapy.
입자 면역반응 분석법을 이용한 축산 사료의 Salmonella와 E.coli 검출을 위한 기초 연구
박하은 ( Haeun Park ),신원진 ( Wonjin Shin ),정지연 ( Jieyun Jung ),박두산 ( Tusan Park ) 한국농업기계학회 2017 한국농업기계학회 학술발표논문집 Vol.22 No.2
국내 송아지에 다발하는 세균성 소화기계 질병들 중 대장균성 설사가 소의 번식 및 단기 비육하는 대부분의 농장에서 문제시 되고 있다. 심한 경우 신생 송아지의 90~100%가 설사증을 보이기도 하고 이들중 20~30%가 폐사하는 경우가 나타나고 있다. 이처럼 설사증을 유발하는 E.coli와 Salmonella는 송아지의 생명에 심각한 위협을 가한다. 그러므로 균 감염에 따른 폐사를 예방하기 위해서는 E.coli와 Salmonella가 송아지 축사에 유입되는 경로를 차단하는 것이 중요하다. 그 일환으로 사료 안의 해당균을 검출하는 시스템을 보편화 할 필요가 있다. 본 연구에서는 입자면역법(Particle Immunoarray)을 통해 균의 농도에 따라 항체 결합 정도를 광학적으로 분석하였다. 입자(Polystyrene Latex)에 각각 E.coli와 Salmonella의 항체를 붙인 후, 이 결합물에 각 항원이 포함되어 있는 시료를 농도 별(10, 10<sup>2</sup>, 10<sup>3</sup> … 10<sup>7</sup> CFU/mL)로 넣어 항원-항체 반응을 일으킴으로써 결합물과 균들을 응집시킨다. 결합물과 균들이 응집되어 있는 정도에 따라 산란광이 다르게 나타나는데, 이를 통해 시료에 포함되어 있던 균의 농도를 측정하는 방법을 사용하였다. 현미경을 통해 항원 시료의 농도 별로 입자가 뭉치는 정도를 시각적으로 관찰하고, 산란광 측정 플랫폼을 통해 입자의 뭉침 정도에 따른 산란광의 차이를 측정하였다. 이를 통해 응집하는 입자의 크기를 광학적으로 검출하는 바이오 센서를 개발하고 또한 사료 안전성 측정에 활용하기 위한 기초연구자료가 되길 기대한다. 더불어 위해 균으로 오염된 사료로부터 이들 균을 추출하는 과정에서 균 추출률을 측정하였다. 균이 사료에 들러붙어 모든 균을 추출하는 것이 어렵기 때문에 그 추출률을 확인해 둘 필요가 있었다. TMR(Total Mixed Ration)사료에 E.coli와 Salmonella를 각각 농도 별(10<sup>3</sup>, 10<sup>4</sup>, 10<sup>5</sup> CFU/mL)로 접종 한 후, 균이 자리 잡을 수 있도록 1시간 동안 상온에서 배양한다. 균이 접종된 사료와 phosphate buffered saline (PBS, pH 7.2)를 1:9의 부피비로 10분 간 혼합한다. 혼합물에서 추출한 균을 배양하여, 초기 균 농도와 사료에서 추출한 균의 농도를 비교하여 추출 과정에서의 균 추출률을 확인하였다. 그 결과 10<sup>3</sup> CFU/mL까지는 추출률이 높았지만 10<sup>4</sup> CFU/mL 이상부터는 낮은 추출률을 보였다.