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세포진 검사상 ASCUS/LSIL이 나온 환자의 치료에 있어서 HPV DNA 검사의 임상적 유용성
이정례 ( Jeong Rye Lee ),송은섭 ( Song Eun Seop ),이정헌 ( Heong Hun Lee ),김세련 ( Se Ryun Kim ),박지현 ( Jee Hyun Park ),황성욱 ( Sung Ook Hwang ),고승권 ( Seung Kwon Koh ),임문환 ( Moon Whan Im ),이병익 ( Byoung Ick Lee ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.2
목적 : PCR을 이용한 HPV DNA 검사법이 비정상 세포진 (ASCUS/LSIL)이 나온 환자에서 고위험 병변을 알아내기 위한 보조적인 수단으로서 임상적인 효용성이 있는지 알아보고자 하였다. 연구 방법 : 1996년 9월 1일부터 2000년 5월 31일까지 인하대병원 산부인과에 내원하여 자궁경부 세포진 검사를 받은 여성 중에서 비정상 세포진 (ASCUS/LSIL)의 결과를 보여 HPV DNA 검사와 질확대경하 조직 생검을 시행 받은 61명을 대상으로 Objective : This study was performed to evaluate the clinical efficacy of high risk HPV DNA test using PCR for the detection of high-grade CIN or invasive cancer from patients with ASCUS or LSIL on Papanicolaou smear. Methods : Sixty one patients with a P
정진현 ( Jin Heon Jeong ),김춘식 ( Choon Sig Kim ),구교상 ( Kyo Sang Goo ),이충화 ( Choong Hwa Lee ),원형규 ( Heong Gyu Won ),변재경 ( Je Geong Byun ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.3
This study was performed to determine the Physicochemical properties of Korean forest soils by parent rock types (Igneous, Metamorphic, Sedimentary rocks) sampled from 915 soil profiles (A horizon : 810, B horizon : 915) between 1984 and 1990. The variables of soil physiochemical were properties significantly correlated among each rock type (P<0.05). Solid percentage in A horizon was significantly correlated (r=0.1384~o,3247) with sand percentage, but particle density was not correlated with soil particle size distribution and soil three phases. Soil pH exhibited high significant correlation (r=0.2283~0.8307) with exchangeable cations (K^+, Na^+, Ca^2+, Mg^2+) in A horizon. Soil chemical properties of B horizon showed similar correlation trends except for metamorphic rocks. Dominant soil texture of two soil horizons (A, B) was loam except for silt loam of A horizon in sedimentary rocks. Soil bulk density in A horizon was 0.84g/cm^3 in metamorphic rocks, 0.91g/cm^3 igneous rocks and 0.97 g/cm^3 sedimentary rocks. Soil particle density was not significantly different among three parent rock types (2.52~2.54 g/cm^3 A horizon and 2.60~2.62g/cm^3 in B horizon). Soil pH in A horizon was pH 5.94 in sedimentary rocks, followed by pH 5.50 in igneous rocks and pH 5.34 in metamorphic rocks. The lowest value in organic matter content was 3.12% in sedimentary rocks, followed by 4.55% in igneous rocks and 4.74% in metamorphic rocks. Available phosphorus was significantly lower in sedimentary rocks (13.2m9/kg) than in metamorphic rocks (27.4mg/kg) and in igneous rocks (28.7mg/kg). Cation exchange capacity (CEC) was 10.34cmol_c/kg in sedimentary rocks. followed by 12.44cmol_c/kg in metamorphic rocks and 13.24coml_c/kg in igneous rocks. Exchangeable cations in A horizon were higher in sedimentary rocks than in igneous or metamorphic rocks. The Korea forest soils by parent rock types showed similar physicochemical properties between igneous and metamorphic rocks, but soil properties of sedimentary rocks were significantly different in soil particle size distribution, soil bulk density, soil pH, organic matter content, total nitrogen, available phosphorus, CEC, Na^+, Ca^2+, Mg^2+, total base, and base saturation compared with both igneous and metamorphic rock types.
토양으로 부터 분리한 방선균 KYR 26균주가 생산하는 항생물질의 분리 정제
유진철,방희재,심정보,이은하,김형근,김성준,김영호,이정준 조선대학교 부설 유전생물공학연구소 1993 유전생물공학연구지 Vol.3 No.-
1. 작용 범위가 넓고 부작용이 적은 의약용 항생물질을 screening하기 위한 목적으로 전남 지역 토양시료로 부터 방선균을 분리하여 항균활성이 우수한 방선균을 선별하여 KYR 26이라 명명 하였다. 2. KYR 26균주의 최적 배양배지는 Modified SMM배지 이었으며, 항생물질 생산은 배양 3일째에 최대의 수율을 나타내었다. 3. KYR 26균주가 생산하는 항생 물질은 Ion exchange chromatography, Adsorption chromatography, LH-20 coluom chromatography, Precipitation과 Silica column chromatography 과정을 통하여 항생물질 A, B를 분리정제 하였다. 4. 분리 정제된 항생 물질 A와 B는 TLC에서 각각 Rf치 0.53, 0.48에서 단일 spot로 나타났고,HPLC에서 항생물질 A와B는 각각 2.94, 7.66의 retention time을 보였으며 모두 98%의 순도를 보였다. 5. 분리 정제된 항생 물질 A와 B의 UV spectrum을 조사한 결과 항생물질 A,B는 모두 270nm에서 최대흡수 파장을 나타내었다. 현재 최종 분리 정재된 항생물질 A와 B의 구조 규명을 위하여 IR, ^1H-NMR, ^3C-NMR, Mass spectroscopy에 관한 실험이 진행중에 있다. Soil Actinomycetes were isolated from soil samples which were collected from the 100 sites of Cheon-nam areas. Isolated strains were tested for the production of antibiotics in various fermentation media using 4 strains of antibiotic sensitive test microorganisms. One strain which has excellent antibacterial activities was selected, and named to KYR 26. From culture broth of KYR 26 strain, two antibiotics were obtained through Ion Exchange Chromatography, Adsorption Chromatography, Gel Filtration Chromatography, Silica gel column chromatography and Crystalization. The finally purified two antibiotics exhibited single spot on the TLC chromatogram,and about 98 % purity on the profile of HPLC Chromatogram. Two antibiotics exhibited Rf 0.53 and 0.48 each on TLC with developing solvent system (MeOH:CHCl_3). The UV spectrum of isolated antibiotics exhibited a characteristic absorption maximum at 270nm.
고추의 육성계통에 따른 Capsaicinoids와 Ascorbic Acid 분석
정미리(Miri Jung),황영(Young Hwang),김혜영(Hae Young Kim),정헌상(Heong-Sang Jeong),박지성(Jisung Park),박동복(Dongbok Park),이준수(Junsoo Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.11
본 연구에서는 130여종의 육성계통 고추의 capsaicinoids와 ascorbic acid 등의 기능성 성분의 함량을 분석함으로써 부가가치 높은 우수 고추 품종 개발을 위한 database를 제공하고자 하였다. Capsaicinoids 및 ascorbic acid 분석을 위해 역상 HPLC를 이용하였다. 연구 결과 capsaicin의 평균함량은 29.0 ㎎/100g, dihydrocapsaicin은 15.8 ㎎/100g으로 나타났으며 ascorbic acid의 평균 함량은 809.8 ㎎/100g이었다. 육성계통 고추 No.2687의 capsaicin 함량은 219.6㎎/100g, dihydrocapsaicin 함량은 110.8 ㎎/100g으로 가장 높게 분석되었으나 ascorbic acid 함량은 264.9 ㎎/100g으로 가장 낮게 분석되었다. 또한 육성계통 고추 No. 2524가 ascorbic acid의 함량이 1695.5 ㎎/100g으로 가장 높았으나 capsaicinoid 함량은 평균 이하로 낮게 나타났다. 분석방법을 검증하기 위해 재현성, 반복성 및 회수율을 구하였으며 그 결과 재현성과 반복성의 CV는 5% 이하였으며 회수율은 90% 이상으로 우수하였다. 본 연구 결과는 고추의 육성 계통별 기능성 성분의 함량을 분석하고 그 결과를 토대로 우수품종 개발을 위한 기초자료를 제공함에 그 의의가 있다. Peppers (Capsicum annuum L.) are a rich source of phytochemicals including polyphenolics, flavonoids, capsaicinoids and ascorbic acid. Capsaicinoids are a group of 12 or more related alkaloids responsible for the pungent sensation in the fruits of the genus Capsicum. Ascorbic acid is another functional and nutritional constituent of peppers. In this study, the contents of two major capsaicinoids (capsaicin and dihydrocapsaicin) and ascorbic acid in 131 pepper breeding lines were quantified by HPLC. In 131 pepper breeding lines, capsaicin and dihydrocapsaicin contents were in the range of 0.0 to 219.6 and 0.0 to 110.8 ㎎/100 g, respectively. The breeding lines with higher capsaicin content contained higher dihydrocapsaicin content as well. Ascorbic acid contents were 264.9 to 1695.5 ㎎/100 g for the 131 pepper breeding lines. The analytical method validation parameters including accuracy, repeatability, and reproducibility were calculated to ensure the method's validity. This study provides basic information to plant breeders and biotechnologists who are planning to breed genotypes with high content of phytochemicals.
Catalytic conversion of 1,2-dichlorobenzene over mesoporous materials from zeolite.
Lee, Hyung Ik,Kim, Ji Man,Lee, Jai-Young,Park, Young-Kwon,Jeon, Jong-Ki,Yim, Jin-Heong,Park, Sung Hoon,Lee, Kyung-Jin,Kim, Seung-Soo,Jeong, Kwang-Eun American Scientific Publishers 2010 Journal of Nanoscience and Nanotechnology Vol.10 No.5
<P>In this study, 1,2-dichlorobenzene (DCB), an important precursor of PCDDs and PCDFs, was chosen as a suitable model compound for the catalyzed deep oxidation of dioxin. The recently developed mesoporous materials from zeolites (MMZ) were used for the first time as a support for an oxidation catalyst. The catalytic oxidation of 1,2-dichlorobenzene over Pt/MMZ was carried out, and the catalytic activity was compared with that of Pt/gamma-Al2O3, Pt/Al-MCM-41 and Pt/Beta catalysts. Pt/MMZ showed the highest catalytic activity among the catalysts tested. Interestingly, the catalytic activity of Pt/MMZ was maintained (> 40%) at low temperatures (250 degrees C) at which the other catalysts showed extremely low activity (< 5%). The high catalytic activity of Pt/MMZ was attributed to both the sufficient acidity and mesoporosity of the MMZ support.</P>