Multi-stage inhibition in breast cancer metastasis by orally active triple conjugate, LHTD4 (low molecular weight heparin-taurocholate-tetrameric deoxycholate)

( Farzana Alam ),( Taslim A. Al Hilal ),( Jooho Park ),( Jeong Uk Choi ),( Foyez Mahmud ),( Jee Heon Jeong ),( In San Kim ),( Sang Yoon Kim ),( Seung Rim Hwang ),( Youngro Byun ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

Targeting multiple stages in metastatic breast cancer is one of the effective ways to inhibit metastatic progression. To target human metastatic breast cancer as well as improving patient compliance, we developed an orally active low molecular weight heparin (LMWH)-taurocholate conjugated with tetra-meric deoxycholic acid, namely LHTD4, which followed by physical complexation with a synthetic bile acid enhancer, DCK. In breast cancer, both transforming growth factor-β1(TGF-β1) and CXCL12 exhibit enhanced metastatic activity during the initiation and progression stages of breast cancer, thus we direct the focus on investigating the antimetastatic effect of LHTD4/DCK complex by targeting TGF- βl and CXCL12. Computer simulation study and SPR analysis were performed for the binding confirmation of LHTD4 with TGF- βl and CXCL12. We carried out in vitro phosphorylation assays of the consecutive re-ceptors of TGF-βl and CXCL12 (TGF-βl R1 and CXCR4, respectively). Effects of LHTD4 on in vitro cell migration (induced by TGF- βl) and chemotaxis (mediated by CXCL12) were investigated. The in vivo anti-metastatic effect of LHTD4 was evaluated in an accelerated metastasis model and an orthotopic MDA-MB-231 breast cancer model. The obtained KD values ofTGF- β1 and CXCL12 with LHTD4 were 0.85 and 0.019 ,βM respectively. The simulation study showed that binding affinities of LHTD4 fragment with either TGF-βl or CXCL 12 through additional electrostatic interaction was more stable than that of LMWH fragment. In vitro phosphorylation assays of TGF-βl R1 and CXCR4 showed that the effective inhibition of receptor phosphorylation was observed with the treatment of LHTD4. The expressions of epithelial to mesenchymal transition (EMT) marker proteins such as vimentin and Snail were prevented by LTHD4 treatment in In vitro studies with TGF-~l treated MDA-MB-231 cells. Moreover, we observed that LHTD4 negatively regulated the functions of TGF-βl and CXCL 12 on migration and invasion of breast cancer cell. In several advanced orthotopic and experimental breast cancer metastasis murine models. the treatment with LHTD4 (5 mgjkg daily. p.o.) significantly inhibited metastasis compared to the control. Overall, LHTD4 exhibited anti-metastatic effects by inhibiting TGF-βl and CXCL12. and the clinically relevant dose of orally active LHTD4 was found to be effective in preclinical studies without any apparent toxicity.

Phenolic Content Analysis of Aloe vera Gel and Evaluation of the Effect of Aloe Gel Supplementation on Oxidative Stress and Fibrosis in Isoproterenol-Administered Cardiac Damage in Rats

Farzana Akther Sumi,Biswajit Sikder,Md Mizanur Rahman,Shamshad Rahman Lubna,Anayt Ulla,Md. Hemayet Hossain,Ismet Ara Jahan,Md Ashraful Alam,Nusrat Subhan 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.3

We evaluated the cardioprotective effect of Aloe vera gel isoprenaline (ISO)-administered myocardial infarction in rats. ISO administration increased lipid peroxidation and oxidative stress in rats, which were ameliorated by A. vera gel supplementation. Our study also revealed that creatine kinase-MB (CK-MB) activities were increased in ISO-administered rats, while the activities of cellular antioxidants, such as superoxide dismutase and catalase, and glutathione concentration were decreased. A. vera gel lowered CK-MB enzyme activities and the glutathione concentration in ISO-administered rats, and increased antioxidant activities. Histopathological examination also revealed increases in thickness of the left ventricle myocardium, increases in mononuclear cell infiltrations, increased degeneration of focal areas of the endocardium, and increased fibrous tissue deposition in the heart of ISO-administered rats; whereas, A. vera prevented infiltration of inflammatory cells and reduced left ventricular fibrosis. In conclusion, we show that A. vera supplementation protects against development of cardiac inflammation, fibrosis, and oxidative stress in ISO-administered rats.

In vivo metabolism study of diuretics clopamide by LC-MS/MS

Farzana Binte Rafique,Anca Raluca Muresan,Khandoker Asiqur Rahaman,Oh-Seung Kwon 한국분석과학회 2020 학술대회논문집 Vol.2020 No.6

The Diversity of Multi-drug Resistance Profiles in Tetracycline-Resistant Vibrio Species Isolated from Coastal Sediments and Seawater

Farzana Ashrafi Neela,Lisa Nonaka,Satoru Suzuki 한국미생물학회 2007 The journal of microbiology Vol.45 No.1

In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two β-lactams, ampicillin (ABPC), and mecillina(MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.

Phenolic Content Analysis of Aloe vera Gel and Evaluation of the Effect of Aloe Gel Supplementation on Oxidative Stress and Fibrosis in Isoprenaline-Administered Cardiac Damage in Rats

Farzana Akther Sumi,Biswajit Sikder,Md Mizanur Rahman,Shamshad Rahman Lubna,Anayt Ulla,Md Hemayet Hossain,Ismet Ara Jahan,Md Ashraful Alam,Nusrat Subhan 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.3

We evaluated the cardioprotective effect of Aloe vera gel isoprenaline (ISO)-administered myocardial infarction in rats. ISO administration increased lipid peroxidation and oxidative stress in rats, which were ameliorated by A. vera gel supplementation. Our study also revealed that creatine kinase-MB (CK-MB) activities were increased in ISO-administered rats, while the activities of cellular antioxidants, such as superoxide dismutase and catalase, and glutathione concentration were decreased. A. vera gel lowered CK-MB enzyme activities and the glutathione concentration in ISO-administered rats, and increased antioxidant activities. Histopathological examination also revealed increases in thickness of the left ventricle myocardium, increases in mononuclear cell infiltrations, increased degeneration of focal areas of the endocardium, and increased fibrous tissue deposition in the heart of ISO-administered rats; whereas, A. vera prevented infiltration of inflammatory cells and reduced left ventricular fibrosis. In conclusion, we show that A. vera supplementation protects against development of cardiac inflammation, fibrosis, and oxidative stress in ISO-administered rats.

Effect of Age of Seedling and Phytohormones on Micropropagation of Indica Rice(Oryza sativa L.) from Meristem Culture

Farzana, Khanum,Husnain, Tayyab,Riazuddin, Sheikh 한국식물학회 1998 Journal of Plant Biology Vol.41 No.2

An efficient protocol for in vitro micropropagation of seven indica rice varieties was developed from meristem culture. Meristem (leaf base) was isolated from different age of seedlings and cultured on MS medium without hormones and supplemented with different concentrations of NAA and BAP. Regeneration of plantlets from meristem was observed within five days of culture. The meristem isolated from 4-day old seedlings gave highest regeneration on hormone free MS medium. Histological study of meristem (leaf base) from 4-day old seedlings confirmed the presence of meristematic cells. Regenerated plants were multiplied on MS medium supplemented with 0.05 ㎎/L NAA and 5 ㎎/L BAP. An average of five plants were obtained from single regenerated meristem. The plants regenerated from meristem showed morphological uniformity.

Effects of feeding corn naturally contaminated with aflatoxin on growth performance, apparent ileal digestibility, serum hormones levels and gene expression of Na+, K+-ATPase in ducklings

Farzana Abbasi,Jingbo Liu,Hongfu Zhang,Xiaoyun Shen,Xuegang Luo 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.1

Objective: A 14-d trial was conducted to determine the effects of feeding corn naturally contaminated with aflatoxin B1 (AFB1) on growth performance, apparent ileal digestibility, serum hormones levels and gene expression of Na+, K+-ATPase in ducklings. Methods: A total of 704 ducklings were blocked on the basis of sex and body weight (BW), and then allocated randomly to one of the following two treatments: i) CON, basal diet and ii) AFB1, diets with 100% of normal corn replaced with AFB1 contaminated corn. There were 22 pens per treatment and 16 birds per pen. The concentration of AFB1 was 195.4 and 124.35 μg/kg in the contaminated corn and AFB1 diet, respectively. Results: The AFB1 decreased average daily gain, average daily feed intake, d 7 BW, final BW in the whole trial, and feed conversion ratio (FCR) during d 8 to 14 and d 1 to 14 by 10% to 47% (p<0.05), while FCR during d 1 to 7 was increased (p<0.05). AFB1 did not affect mortality to 7 d of age, and then increased to 5.8% from 8 to 14 d of age (p<0.01). Apparent ileal gross energy digestibility was reduced by AFB1, whereas apparent ileal digestibility of dry matter, nitrogen, and amino acid was improved (p<0.01). Feeding AFB1 diets increased serum concentration of leptin and insulin-like growth factors-1 (IGF-1) (p<0.05), but had no effect on neuropeptide Y, ghrelin, cholecystokinin-8 or insulin (p>0.05). Dietary treatments did not influence relative expression of jejunal Na+, K+-ATPase gene (p>0.05). Conclusion: Taken together, feeding corn naturally contaminated with AFB1 reduced growth performance, improved apparent ileal digestibility, and affected serum leptin and IGF-1 in ducklings from d 1 to 14.

Current Trends in Studies on Reverse-Mode Polymer Dispersed Liquid-Crystal Films – A Review

Farzana Ahmad,M. Jamil,Y.J. Jeona 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.4

Reverse-mode polymer dispersed liquid crystals (PDLCs) comprise an important new class of materials for optical device applications. Generally reverse-mode PDLCs are transparent and opaque in the absence and presence of an external field, respectively. Display devices based on reverse-mode PDLC technology are useful for largearea displays; because their fabrication for manufacturing shutters is considered to be easier and faster, they are also employed for automotive technology and smart windows. These devices can be operated at a low voltage, which conserves energy in intelligent-device applications. This work presents a comprehensive review of past research regarding reverse-mode PDLCs and includes the advantageous features, applications, and various fabrication methods of reverse-mode PDLCs and photo-chromic reverse-mode PDLCs. In addition, some new features of this technology that have recently been reported and future investigations by a variety of research groups are presented.

The Effect of UV Intensities and Curing Time on Polymer Dispersed Liquid Crystal (PDLC) Display: A Detailed Analysis Study

Farzana Ahmad,Muhammad Jamil,이진우,김서록,전영재 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.5

In current study polymer dispersed liquid crystal (PDLC) films whosecomposition based on nematic liquid crystal (LC) E7 and prepolymericNOA65 were formed via the photo induced phaseseparation method, in a wide intensity range of the UV light (I = 0.33-1.8 mW/cm2) and curing duration (t = 120-600 sec). The PDLCcharacteristics were monitored by surface morphology, electro opticalstudies, as well as by phase separation process through measuring theFTIR absorption of the composite layers. Increase of curing lightintensity accelerates the phase separation and drastically influencesthe final morphology of LC droplets inside PDLCs. Likewise bywidening the curing duration the enhancement in phase separationwas observed. Increase of light intensity from 0.89 mW/cm2 andduration t = 120-240 sec resulted into transition from large LCdomains of irregular shape (due to aggregation of droplets) to finemono dispersed LC droplets. This morphology caused increase inoptical scattering on zero voltage and high driving voltage. Howeverunexpectedly, this response was not directly related with the curingconditions (intensity and time). These findings extend the potentialapplications of thiol-ene based PDLCs.

A Configurable Fault Tolerant Architecture for Component-Based Systems

Farzana Yousuf,Aamer Nadeem,Zahid Zaman 보안공학연구지원센터 2009 International Journal of Software Engineering and Vol.3 No.2

Component-based software engineering is an emerging paradigm for software development. Benefits of component-based development include significant reduction in the development cost, time and improvement in the dependability requirements. Commercial off-the-shelf (COTS) components are used without any code modification and inspection. When such components are integrated to build safety-critical systems, faults within individual COTS components or faults due to their collaboration may cause catastrophic failures. Therefore, for systems with high dependability requirements, it is essential to incorporate ways for tolerating the software faults at architectural level to deal with the faults that are not catered within the components. The existing component based fault tolerant architectures provide fault tolerance through either exception handling or design diversity. In this paper, we propose a fault tolerant architecture, which supports design diversity and exception handling fault tolerance strategies. The proposed fault tolerant component architecture employs special-purpose connectors called design diverse-multiple version connectors (DD-MVC). These connectors allow design diverse N-variants of COTS to run in parallel. Moreover, proposed architecture also has fault tolerant architectural level connector. The proposed architecture can be configured to adjust the tradeoff between dependability and efficiency and exhibits the ability to tolerate the anticipated and unanticipated faults effectively. The applicability of proposed architecture is demonstrated with a prototype implementation and a case study.