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      • 케미컬오일을 이용한 ERF 연마 특성

        윤종호,이재종,이응숙,이동주,김영호 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

        Electro-rheological fluid is recently used for the micro polishing of 3-dimensional micro-aspherical lens. It's also used for polishing small area defects on the wide flat wafer. Since ER fluid shows a behavior of viscosity changing under certain electric fields, micro polishing efficiency may be enhanced for certain cases. In this paper, a perfluorinated carbonyl fluoride oil based ER fluids was used to improve surface polishing rate and submicron-scale accuracy. As the polishing electrodes, micro size cylindrical tools had been used for maximizing the electric field. An experimental device, which was applied for micro polishing a number of wafers of 4 inches in size and other workpieces, was made on a precision polishing system. It consisted of a steel electrode, a wafer fixture, 10mA current and DC 5kV power supply unit, and a controller unit. From the Experiments, the ER fluid is applicable for micro polishing of small parts.

      • 굴비 製造中의 核酸關聯物質의 變化

        李應昊,金洙賢 釜山水産大學校 1975 釜山水産大學 硏究報告 Vol.14 No.2

        굴비 製造中의 一般成分, 아미노態窒素, 總窒素 및 核酸關聯物質의 變化를 實驗하여 다음과 같은 結果를 얻었다. 1. 原料 참조기에는 IMP와 inosine含量이 월등하게 많았고, ATP, ADP, AMP및 hypoxanthine은 적었다. 2. 마른간 工程中, 原料 참조기중에 많았던 IMP(13.7 μmloe/g, dry base) 는 약 1/9로 inosine (12.9μmole/g, dry base)은 1/3로 減小하였고, 한편 hypoxanthine은 5.7培로 增加하여 19.2μmole/g, dry base였다. ATP, ADP 및 AMP는 큰 變化가 없었다. 마른간 한 後 굴비를 製造하기 위한 乾燥工程에는 鹽藏直後에 많았던 hypoxanthine은 約 1/2로 減小하여 10.2μmole/g. dry base였다. 굴비 중에는 hypoxanthine 含量이 가장 많고, ATP, ADP, AMP, IMP��inosine은 量이 아주 적었다. 3. 굴비 製造中 脫水는 主로 鹽藏工程中에 일어 났으며, 아미노態窒素는 原料 참조기에 乾物重量基準으로 136㎎%이던 것이 鹽藏直後에 298㎎%로서 鹽藏工程中 2.2倍 增加하였고, 굴비 第品은 354mg%로서 鹽藏後 굴비를 製造하기위한 乾燥工程中에 다시 增加하며 原料에 비하여 2.6倍 增加하였다. 4. 굴비의 獨特한 맛에 核酸關聯物質은 큰 구실을 하지 않는다고 보아지며, 아미노態窒素가 굴비 製造工程中 월등히 增加하는 것으로 보아 呈味性 아미노산 및 肉組織이 큰 구실을 할 것으로 보아진다. "Gulbi", salted and dried yellow corvenia, is one of nation-widely consuming fish foods in this country. In this paper, the degradation of nucleotides and their related compounds in yellow corvenia during "Gulbi" processing was studied. The nucleotides and their related compounds were extracted with cold perchloric acid and the amounts were determined by anion exchange column chromatography. In raw yellow corvenia, the results showed that IMP and inosine were dominant and the content of the former was 42.4% and the latter was 39.9% of total nucleotides and their related compounds, and hypoxanthine was 10.4% while the content of ATP, ADP and AMP were low. The IMP and inosine tended to degrade rapidly and hypoxanthine increased remarkably during cry salting for 14 days. After salting and drying, hypoxanthine remained more than the other nucleotides and their related compounds, and ATP, ADP and AMP hardly changed. After "Gulbi" processed, the content of hypoxanthine was the highest whereas ATP, ADP, AMP, IMP and inosine were lower. Free amino nitrogen increased to 2.6 times of that in raw muscle during "Gulbi" processing. As a conclusion, it is presumed the characteristic flavor of "Gulbi" is not attributed to the nucleotides and their related compounds but free amino acid and texture of muscle.

      • Polyethylene glycol 용액(Colyte^(R))을 복용한 후 발생한 Boerhaave 증후군 1예

        이관행,기승석,김인숙,김민정,박인호,장세중,하지수,김응수 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

        Spontaneous esophageal rupture (Boerhaave's syndrome) is a rare case that require early diagnosis and treatment because of its high mortality. The oral administration of osmotically balanced polyethylene-glycol-based electrolyte preparation is a commonly prescribed preparation for cleansing in patients undergoing colonoscopy. Nausea, vomiting and abdominal pain are frequently seen, but serious adverse reactions are rare. Esophageal rupture secondary to severe vomiting, which occurred during colonoscopy preparation using polyethylene gIycoI(PEG) electrolyte solution is extremely rare and only few cases have been published in the literature. We report a case of spontaneous esophageal rupture after routine administration of the PEG before colonoscopy. 대장 정결을목적으로 PEG 사용 후 아주 드물게 발생하는 Boerhaave 증후군 1예를 경험하였기에 보고하는 바이다.

      • 세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

        이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

        Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

      • KCI등재
      • 멸치젓의 呈味成分

        李應昊,金世權,錢重均,金洙賢,金程均 釜山水産大學校 1982 釜山水産大學 硏究報告 Vol.22 No.1

        멸치젓의 呈味成分에 관한 기초자료를 얻고자 생멸치와 이를 原料로 하여 제조된 멸치젓 중의 核酸關聯物質, 유리아미노산, betaine, TMAO, TMA 및 總creatinine을 分析하였다. 核酸關聯物質 중 생멸치에서는 IMP가 乾物量基準으로 2.8 μmole/g로 가장 많았으나 멸치젓에서는 hypoxanthine이 4.2 μmole/g으로 가장 많았고 , ATP는 흔적량에 불과 하였다. 멸치젓 중의 유리아미노산 중 함량이 많은 것은 leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine의 順이었고 glycine, proline, glutamic acid, arginine, threonine, serine 등은 함량이 적었다. 그리고 全유리아미노산은 젓갈 숙성중생원료에 비하여 약 2배로 增加 하였다. Betaine 은 생멸치 및 멸치젓 중에 各各 10.2㎎/100g, 30.0㎎/100/g였으며 이들이 엑스分室素에 대한 比率은 各各 0.6% 및 1.2%였다. TMAO 含量은 생멸치가 13.9㎎/100g, 멸치젓이 9.0㎎/100g였으며 TMA 含量은 各各 22.7㎎/100g 및 30.9㎎/100g였다. 總 creatinine 含量은 생멸치 및 멸치젓에서 各各 433.1㎎/100g, 575.8㎎/100g로서 엑스分室素에 대해 27.2% 및 22.8%였다. Omission test 結果 멸치젓의 呈味成分으로서는 유리아미노산 및 核酸關聯物質이 중요한 구실을 한다는 것을 알았다. This study was conducted to evaluate the taste compounds of fermented anchovy. The contents of such compounds as nucleotides and their related compounds, free amino acids, betaine, TMAO and total creatinine raw and fermented anchovy were analysed. The contents of IMP in raw anchovy appeared higher than other nucleotides and tended to decrease rapidly during fermentation. Therefore, in the case of fermented anchovy, hypoxanthine was abundant and IMP was very low in content. In the free amino acid composition of fresh samples abundant amino acids were leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine in order. Such amino acids as glycine, proline, glutamic acid, arginine, threonine and serine were poor in content. The contents of total free amino acids reached approximately about two times as compared with that of raw sample. The amount of betaine and TMA increased, while TMAO decreased during fermentation. The content of total creatinine in raw and fermented anchovy was occupied 22.8% and 27.2% of total of extractive nitrogen, respectively. As the results of omission test, the taste-active compounds of fermented anchovy are assumed to be free amino acids and nucleotides.

      • 까치복의 呈味成分

        李應昊,鄭秀烈,趙舜榮,錢重均,車庸準 釜山水産大學校 1983 釜山水産大學 硏究報告 Vol.23 No.1

        까치복의 呈味成分에 관한 資料를 얻고자 核酸關聯物質, 유리아미노산, TMAO, TMA, betaine 및 총 creatinine을 分析하였다. 核酸關聯物質 중 IMP가 1.9μmole/g으로서 가장 많았고, 全核酸關聯物質에 대해 39.6%를 차지하였다. 유리아미노산 중 함량이 많은 것은 taurine, lysine, alanine 및 glycine 이고, 이들 네 가지 아미노산이 전유리아미노산의 68.2%를 차지하였다. 총 creatinine�량은 194.5mg/100g으로서 엑스分窒素에 대해 63.4%를 차지하였으며, TMAO 및 betaine 함량은 微量이었다. Omission test 結果 까치복의 呈味成分은 5'-mononucleotides 및 유리아미노산이 주된 구실을 한다는 것을 알았다. Yellowfin puffer, Fugu xanthopterus(Temminck et Schlegel), is one of the most palatable fishes in Korea. However, relatively little is known about the characteristics of its taste. In this study, the taste compounds including nucleotides and their related compounds, free amino acids, trimethylamine oxide, trimethylamine, betaine and total creatinine were analyzed, and their roles in tasting activity were investigated. The amounts of IMP was 1.9 μmole/g, and the ratio of IMP to the total nucleotides and their related compounds was 39.6%. The great portion of free amino acids in the extractives of yellowfin puffer muscle was occupied by taurine, lysine, alanine and glycine in order, and their content was 79.1% of the total free amino acids. Among the organic bases, total creatinine was abundant, and its nitrogen content was 63.4% of total extractive nitrogen. According to the results of the omission test, the main constituents of the characteristic taste of yellowfin puffer could be assumed as free amino acids and 5'-mononucleotides.

      • 마산 수출자유지역공단 근로자들에 대한 사회의학적 조사연구

        이영호,정석주,이수일,김응남,맹광호 가톨릭대학산업의학센타 산업의학연구소 1977 韓國의 産業醫學 Vol.16 No.3

        Followings are major findings obtained from a study on the socio-medical studus of the manu-facturing workers in the Masan Free Export Zone. This study was planned to find out socio-medical conditions of the workers which seemed to be essential to the better management of the health of the workers and the working environments. For this study, 6978 workers who are equivalent to 26.9% of total workers in Masan Free Export Zone as of the end of 1976 were sampled and they were asked to answer to the pretested questionnaire. 1) General characteristics; (1) 83.9% of sampled workers were female. (2) 84.9% of workers were less than 25 years of age and female workers who entered in this age group were 93.6%. (3) 91.9% of workers had more than junior high school education and those who finished only junior high school occupied the majority with the proportion of 67.7%. (4) 897.1% of workers were un-married and the proportion for the female was 93.2% (5) Majority (89.6%) were from Yongnam area and 5.2% of workers were from Honam area. (6) 49.1% of workers were living separately from their families and 76.9% of them were self-cooking. 2) Working conditions and working history; (1) 78.1% of workers were receiving from 20,000 won 40,000won as their monthly salaries and those who were receiving from 40,000 won to 60,000 won were the next (12.5%). (2) 34.4% of workers had been working in the present plant for from one to three years and those who had been working for less than one year were 31.9%. (3) Most of workers (91.2%) were working 8 hours a day but 25.3% of precision optical instruments manufacturing workers were working about 10 hours a day. (4) Only 6.2% of workers ever changed their working places during the past one year. 89.0% of them changed once and 9.0%, twice. Male workers changed their working places more often (8.7%) than female workers (5.7%). 3) Present and past history of illness; (1) 6.7% of workers had the sick among their family members and cardiovascular diseases were most common (47.8%) (2) Those who had experiences of sickness lasted more than one week were 7.1% and those who had had operations were 3.3%. (3) 42.0% of workers were complainning at least one physical symptom. Those who were complainning "tingling pain on eye ball" were the most (20.5%), and headache, and dizziness were both the next (16.3%). 4) Smoking, drinking habits and drug dependency; (1) Among all workers, 11.7% (male, 69.5%; female, 0.4%) were smoking and 13.1%(male, 71.9%; female, 2.3%) were drinking alchole. Those who were using drug customarily were only 2.3% and the drugs they were using were aspirin (35.0%), antibiotics (12.5%) and others. 5) Present physical conditions found at the periodic physical examinations; (1) Mean height of male workers was 168.3cm and that of female workers was 158.1cm. Mean body weight of male workers was 60.7kg and that of female workers was 51.3kg. (2) Those who had anormal vision were 10.1% and the frequency of self-awared hearing loss was 4.5%.If the visual power of one or both eyes was less than 0.6, we considered this as and abnormal vision. (3) Those who were informed by the physician that they had hypertension were 8.4% and those whe were informed that they had tuberculosis lesion on the chest were only 0.4%.

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