http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Eun Kyung Cho,Mi Ok Lee,Young Ju Choi 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2
In this study, Cyclophilin isolated from cDNA library for Griffithsia japonica among the red algae was named as GjCyp-1 and we investigated the function and characterization of GjCyp-1. For studying the function of the gene, a recombinant GjCyp-1 containin
Prognostic Value of AML1/ETO Fusion Transcripts in Patients with Acute Myelogenous Leukemia
( Eun Kyung Cho ),( Soo Mee Bang ),( Jeong Yeal Ahn ),( Seung Min Yoo ),( Pil Whan Park ),( Yieh Hea Seo ),( Dong Bok Shin ),( Jae Hoon Lee ) 대한내과학회 2003 The Korean Journal of Internal Medicine Vol.18 No.1
Background: The t (8;21) (q22;q22), which produces the fusion gene AML1/ETO, is associated with relatively good prognosis and, in particular, with a good response to cytosine arabinoside. Analysis of t (8:21) positive leukemic blasts has shown characteris
Eun Kyung Cho 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5
Members of the cyclophilin (Cyp) family are known to function as co-chaperones, interacting with chaperones such as heat shock protein 90, and perform important roles in protein folding under high temperature stress. In addition, they have been isolated from a wide range of organisms. However, there have been no reports on the functions of algal Cyps under other stress conditions. To study the functions of the cDNA GjCyp-1 isolated from the red alga (Griffithsia japonica), a recombinant GjCyp-1 containing a hexahistidine tag at the amino-terminus was constructed and expressed in Escherichia coli. Most of the gene product expressed in E. coli was organized as aggregate insoluble particles known as inclusion bodies. Thus, the optimal time, temperature, and concentration of L(+)-arabinose for expressing the soluble and nonaggregated form of GjCyp-1 in E. coli were examined. The results indicate that the induction of Cyp, at 0.2% L(+)-arabinose for 2 h at 25℃, had a marked effect on the yield of the soluble and active form of the co-chaperone as PPIase. An expressed fusion protein, H6GjCyp-1, maintained the stability of E. coli proteins up to -75℃. In a functional bioassay of the recombinant H6GjCyp-1, the viability of E. coli cells overexpressing H6GjCyp-1 was compared to that of cells not expressing H6GjCyp-1 at -75℃. For all the cycles of a freeze/thaw treatment, a significant increase in viability was observed in the E. coli cells overexpressing H6GjCyp-1. The results of the GjCyp-1 bioassays, as well as in vitro studies, strongly suggest that the algal Cyp confers freeze tolerance to E. coli.
On Quaternion Group Rings over a Field
( Eun-kyung Cho ),( Yang Lee ) 한국수학교육학회 2016 수학교육 학술지 Vol.2016 No.1
In this note, we will give a survey on some well known facts related to a quaternion group rings over a field and add some more facts that has been newly discovered. It is known that for R=KQ(8) , where K is a field of characteristic P=0, R is duo if and only if R is unit-duo if and only if R is symmetric if and only if R is reversible if and only if R is semicommutative if and only if R is Abelian if and only if the equation 1+X(2)+Y(2)=0 has no solution in R by [2], [5], [6]. Here, we can add the condition that R is reduced to this equivalent conditions. Then we prove that the above relationships also hold for R=KQ(8) where K is a field of characteristic P>2. In particular R is neither of them all.
A Cyclophilin from Griffithsia japonica Has Thermoprotective Activity and Is Affected by CsA
Eun Kyung Cho,Yoo Kyung Lee,Choo Bong Hong 한국분자세포생물학회 2005 Molecules and cells Vol.20 No.1
Members of the multifunctional Cyp family have been isolated from a wide range of organisms. However, few functional studies have been performed on the role of these proteins as chaperones in red alga. For studying the function of cDNA GjCyp-1 isolated from the red alga (Griffithsia japonica), we expressed and purified a recombinant GjCyp-1 containing a hexahistidine tag at the amino-terminus in Escherichia coli. An expressed fusion protein, H6GjCyp-1 maintained the stability of E. coli proteins up to 50°C. For a functional bioassay for recombinant H6GjCyp-1, the viability of E. coli cells overexpressing H6GjCyp-1 was compared with that of cells not expressing H6GjCyp-1 at 50°C. After high temperature treatment for 1 h, E. coli overexpressing H6GjCyp-1 survived about three times longer than E. coli lacking H6GjCyp-1. Measurement of the light scattering of luciferase (luc) showed that GjCyp-1 prevents the aggregation of luc during mild heat stress and that the thermoprotective activity of GjCyp-1 is blocked by cyclosporin A (CsA), an inhibitor of Cyps. Furthermore, the Cyp-CsA complex inhibited the growth of E. coli under normal conditions. The results of the GjCyp-1 bioassays as well as in vitro studies strongly suggest that Cyp confers thermotolerance to E. coli.
( Eun Kyung Cho ),( In Soon Choi ),( Young Ju Choi ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.3
The chitinase-producing strain SC081 was isolated from Korean traditional soy sauce and identified as Bacillus atrophaeus based on a phylogenetic analysis of the 16S rDNA sequence and a phenotypic analysis. A gene encoding chitinase from B. atrophaeus SC081 was cloned in Escherichia coli and was named SCChi-1 (GQ360078). The SCChi-1 nucleotide sequences were composed of 1788 base pairs and 596 amino acids, which were 92.6, 89.6, 89.3, and 78.9% identical to those of Bacillus subtilis (ABG57262), Bacillus pumilus (ABI15082), Bacillus amyloliquefaciens (ABO15008), and Bacillus licheniformis (ACF40833), respectively. A recombinant SCChi-1 containing a hexahistidine tag at the amino- terminus was constructed, overexpressed, and purified in E. coli to characterize SCChi-1. H6SCChi-1 revealed a hydrolytic band on zymograms containing 0.1% glycol chitin and showed the highest lytic activity on colloidal chitin and acidic chitosan. The optimal temperature and pH for chitinolytic activity were 50℃ and pH 8.0, respectively. [BMB reports 2011; 44(3): 193-198]