Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

대장내시경 전처치제로서 Sodium Phosphate 와 Polyethylene Glycol 용액의 전향적 비교 분석

이헌경,김승용,홍원선,민영일,정훈용,김해련,정성애,김석균,심기남,양석균,박의련,조문경 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.3

Background/Aims: Although some authors have suggested that sodium phosphate (NaP) is more effective than polyethylene glycol (PEG) in bowel cleansing, there has been no crossover study proving the superiority of NaP over PEG in bowel cleansing and patients' compliance. The aim of this study was to compare the two solutions for colonoscopy, PEG and NaP, through crossover design with regard to patients' compliance, cleansing ability and side effects. Methods: Thirty patients underwent two separate colonoscopies for colonic polyp(s) with PEG and NaP, respectively. Before and after bowel preparation, blood pressure, body weight, and serum biochemical parameters were measured in all patients. In addition, a detailed questionnaire was used to assess side effects and the patients' preference. The presence of bubbles, types of residual stool, and overall quality of colon cleansing were assessed by one endoscopist blinded to the type of preparation used. In each colonoscopy, two biopsy specimens were taken at rectum. Results: In the NaP group, but not in the PEG group, there were significant changes in several biochemical parameters including sodium (+3.0±3.0 mEq/L), potassium (-0.3±0.3mEq/L), calcium (-0.5±0.5 mg/dL), phosphorus (+3.9±2.2 mg/dL) and osmolarity ( +10.1±9.3 mOsm/kg) after bowel preparation. In addition, the degree of body weight change was greater with NaP (-2.2±2.3 kg) than with PEG (-1.2±2.0 kg) (p=0.06) and the formation of bubbles that disturb luminal observation was more frequently found in the NaP group (p$lt;0.01). There was no difference, however, in the type of residual stool and the overall quality of bowel preparation between the two groups and no significant mucosal change was noted after bowel preparation in both groups. Moreover, PEG was found to be more difficult to take than NaP (p$lt;0.05) and among the 30 patients, 26 (87%) preferred NaP, while only two favored PEG (p$lt;0.01). Conclusions: We conclude that NaP can replace PEG at least in patients with good general condition. Further studies to decrease the incidence of bubbles and to establish subgroups suitable for NaP are needed.

발전기 고정자 권선용 마이카/에폭시 수지의 표면 및 구조분석

이상교,심재선,정의남,송우창,박하용,심상흥,임윤희,박종국,심낙순 三陟大學校 1998 論文集 Vol.31 No.1

The purpose of this study is to determine the ageing mechanism of Mica/Epoxy composite material for large generator stator windings in order to estimate remaining life of generator. X-ray analysis is performed to investigate the position and structure of mica crystal in insulation materials. The phase change of aged and sound specimen under each atmosphere isn't observed. Surface analysis of insulation materials by optical microscope also show that the sound and aged specimen, sliding phenomenon by shear and thermal stress is observed both interface between mica and epoxy.

PTW를 이용한 수변전 계통의 고조파 특성 분석

李懿鏞,楔龍泰 湖西大學校工業技術硏究所 1999 工業技術硏究所論文集 Vol.18 No.-

In this paper the harmonics characteristics were analysed in industrial power systems using PTW(Power Tool Window). Some unknown input data, cable and transformer, were estimated by the approximation method. It is found that the high level harmonics are mainly caused by electric power conversion equipments used for rotating loads. Especially, it was observed that the maximum voltage waveform distortion rate appears at the low voltage bus for DC drive and the voltage and current waveforms are much irregular than those of the other loads.

노후공동주택의 거주성능평가요소에 관한 연구

이병태,현택수,정의용 대한건축학회 2004 대한건축학회논문집 Vol.20 No.2

This study aims to propose the comprehensive evaluation of Performance multi-family housing. Most of the existing studies for the reproduction have evaluated the deterioration by the safety of structure. Therefore those pass over the functional factors which is important for the evaluation of residential qualities. This study represents that the reproduction renovation should not be secured the safety of structure but established the quality of life. And questionnaire asking expert's opinion also supplemented. As a result, the item for examination and the weight for evaluation are suggested in functional deterioration. The results of this study are summarized as follows: 1. Existent redevelopment criteria are not appropriate to decide the environmental comfort, the vital function of housing and the residential management finance. 2. The future criteria have to evaluate the existing environmental comfort, be reconstructed to put first general deterioration. 3. Evaluation elements was extracted throughout the document research. evaluation elements analysis system was separated in residential area, suburb of dwellings, community, and housing complexity environmental assesment. 4. Each category of influence ratio was extracted to assess total deterioration throughout the expert question survey. 5. Each category analysed : ① 0~30%: function is good (unnecessary to improve) ② 31~40%: average (necessary of partial improvement) ③ 41~59%: bad (necessary of total improvement) ④ 60~100%: the worst (unable to improve throughout the repair)

軟弱地에서 動力耕耘機 沈下量 推定을 위한 平板의 適正形象 및 크기에 관한 硏究

李揆昇,李鎔國,趙義健 成均館大學校 科學技術硏究所 1986 論文集 Vol.37 No.1

This study was conducted to determine the optimum shape and dimension of plate predicting the sink age of power tiller in soft paddy field. Various size of disc and rectangular plates were tried at five different soil moisture content, and the resules are summarized as follows. 1. Linear relationship is recognized between contact pressure and sinkage of plates using log-log paper at higher soil moisture content, but the plate sinkage is affected by hard pan in case of the lower moisture soil conditions. 2. Concerning the value of soil deformation coefficient K, the variation of measured values due to shape and size of plate can be practically negligible for the rectangular plates which have 2.5, 5 and 10cm width and with 2 or 3 aspect ratio, and the disc plate which has 20cm diameter. The variation of the exponent of sinkage n, can be practically negligible for the rectangular plates which have 5, 10 and 15cm width, and disc plates which have 20 and 30cm diameters. 3. Relationship between the sinkage of some type of plate and that of power tiller was linear. The closest relationship were found between the sinkage of power tiller and plates for 2.5 and 5cm width rectangular plates regardless of aspect ratio and for the disc plates having 10 and 20cm diameters. 4. Consequently, the optimum plate shape and size for predicting the sinkage of power tiller in soft paddy field are found as the rectangular plates which have 5 and 10cm width and 2 or 3 aspect ratio, and the disc plates having 10 and 20cm diameter.

운동 중 지방 대사에 대한 고찰

이근일,백병주,김의영,김도준,조용철,정훈,조재혁 龍仁大學校 體育科學硏究所 2002 體育科學硏究論叢 Vol.12 No.1

Glucose-fatty acid cycle is very important because it explains that fatty acid influences glucose level. Glucose-fatty acid cycle is the key factor to understand how endurance training which aids fat oxidation and carbohydrate stored in skeletal muscle influence mitochondria level. However, it is not certain whether glucose-fatty acid cycle influences exercising skeletal muscle of human being. As an energy resource, training increases use of fatty acid, endurance ability, and oxidation of triglyceride. During high level training, catecholamine secretions and restraint of fatty acid use caused by insulin increases dramatically, this results in reduction of free fat acids and reunification of free fat acids into triglyceride. Training restrains insulin activity and reduces plasma acid density, however, elite athletes have high triglyceride-fatty acid cycle. Although enzyme's activities and mitochondira's density increase after training, it's not certain that enzyme's activities is major factor in restraint of fatty acid oxidation in exercising muscle cells. Stores of fat leads to increases of fat oxidation, performance and reduces use of carbohydrate, however, there is no absolute evidence to support these results.

廢棄自然皮革을 利用한 新製品의 시스템的 開發

李得熙,玄義泰,鄭旻溶 건국대학교 1976 學術誌 Vol.20 No.1

방사선조사가 Porphyromonas gingivalis에 미치는 영향

이창환,김규태,최용석,황의환 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.1

Purpose : The aim of this study was to observe a direct effect of irradiation on the periodontopathic Porphyromonas gingivalis (P. gingivalis). Materials and Methods : P. gingivalis 2561 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40 Gy. Changes in viability and antibiotic sensitivity, morphology, transcription, and protein profile of the bacterium after irradiation were examined by pour plating method, disc diffusion method, transmission electron microscopy, RT-PCR, and immunoblot, respectively. Results : Viability of irradiated P. gingivalis drastically reduced as irradiation dose was increased. Irradiated P. gingivalis was found to have become more sensitive to antibiotics as radiation dose was increased. With observation under the transmission electron microscope, the number of morphologically abnormal cells was increased with increasing of irradiation dose. In RT-PCR, decrease in the expression of fimA and sod was observed in irradiated P. gingivalis. In immunoblot, change of profile in irradiated P. gingivalis was found in a number of proteins including 43-kDa fimbrillin. Conclusion : These results suggest that irradiation may affect the cell integrity of P. gingivalis, which is manifested by the change in cell morphology and antibiotic sensitivity, affecting viability of the bacterium.