Proteus 균종에서 플라스미드 pBluescript의 전기적 재결합에 관한연구

최향경,조지현,문영희 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

In order to genetic analysis of microorganisms, electroporation method was applied to induce electrotransformation of two strains of Proteus mirabilis (API code: 0536000 and 0737000). isolated from patients at Wonkwang University Hospital. In this study, we tried to evaluate the effect of parameters, ie amount of competent cell and plasmid DNA(pBluescript) on the survival of transformant and transformation efficiency and examine antibiotic susceptibility patterns and biochemical properties of tested strains before and after electroporation. The results were as follows: 1. The effect of amount of competent cells and plasmid DNA to transformation efficiency were different between strains. But the highest efficiency was noted at the amount of pBluescript 20ng. 2. In order to confirm the transformant, pBluescript before and after electroporation were isolated from colonies on the LB agar containing ampicillin and electrophoresed. Two plasmids showed same sized DNA. 3. Antibiotic susceptibility to ampicillin was changed from susceptible to resistant in both strains and to carbenicillin. changed from susceptible to intermediate.

알레르기 환자에서 면역요법에 따른 면역학적 지표의 변화

최영환,허영구,김항재,김능수 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

혼합 항원추출액으로 면역요법시 경과 관찰에 도움이 되는 객관적인 지표를 설정하기 위하여 D. farinae 또는 D. pteronyssinus를 포함한 2내지 6가지 복수의 기인 항원을 갖고 면역요법을 6∼12개월 받은 환자(제Ⅱ군)와 2년 이상 받은 환자(제Ⅲ군)를 대상으로 말초혈액 호산구수, 혈청총IgE치, RAST score, 피부반응도, 말초혈액 IgE수용체보유림프구, 말초혈액 T, B림프구 및 T림프구 아형을 측정하여 면역요법을 받지 않은 알레르기 환자(제Ⅰ군)들에서의 성적과 비교 검토하였다. 혈청총IgE치는 6∼12개월 면역요법군 및 2년이상 면역요법군에서 면역요법을 받지 않은 군보다 유의하게 낮았다. (각각 P<0.05) 면역요법전혈청총IgE치가 400IU/㎖이상인 환자에서는 면역요법후 그 감소가 뚜렷하였으나 300IU/㎖이하인 경우는 유의한 감소를 볼 수 있다. 면역요법을 받지 않았는 군에서의 피부반응도지수의 평균은 1.80±1.79였고 6∼12개월 면역요법을 받은 제Ⅱ군 및 2년이상 면역요법을 받은 제Ⅲ군의 피부반응도지수는 제Ⅰ군보다 각각 유의하게 낮았다. 말초혈액 FcεR(+)림프구는 면역요법을 받지 않았는 Ⅰ군보다 Ⅱ군 및 Ⅲ군에서 유의하게 낮았으나 여전히 정상치 이상이었다. 그러나 혈중 호산구수, RAST score, 말초혈액림프구 및 그 아형은 면역요법 전후 유의한 변화를 나타내지 않았다. 이상의 성적에서 혈청총 IgE치, 피부반응도지수, 말초혈액FcεR(+)림프구는 면역치료의 경과를 대체로 추적할 수 있는 지표가 될 수 있을 것으로 생각된다. To determine the reliable parameters for assessing the efficacy of immunotherapy in multi-allergen sensitive allergy patients, we observed the changes of peripheral blood eosinophil counts, total and specific IgE levels, skin-test reactivity index, Fcε receptor-bearing lymphocytes, T/B lymphocytes and T-subsets in peripheral blood in the three groups of allergic asthma or rhinitis patients, the first group(group Ⅰ) of 102 patients who were not on immunotherapy, the second group(group Ⅱ) of 28 patients who received 6-12 months immunotherapy, and the third one(group Ⅲ) of 24 patients who received 2 years immunotherapy. Those patients were sensitive to 2-6 aeroallergens including D. farinae or D. pteronyssinus and all the patients in group Ⅱ and group Ⅲ had been immunized with the specific desensitization vaccine (SDV, Bencard®), resulting good response on symptoms. Total serum IgE levels were significantly decreased in the groups of Ⅱ and Ⅲ, compared with groupⅠand the decrease in IgE was more prominent in the patients of high IgE level over 400IU/㎖ prior to immunotherapy. The mean of skin-test reactivity index was 1.80±0.79 in group Ⅰ and this index was significantly lowered in group Ⅱ and Ⅲ. Significant decrease in Fcε receptor-bearing lymphocytes in peripheral blood was developed in immunotherapy-received groups(Ⅱ and Ⅲ). However, the patients still had higher levels of Fcε receptor-bearing lymphocytes in peripheral blood than normal controls, even after more than 2 years of immunotherapy. The differences of peripheral blood eosinophil counts, RAST score, T/B lymphocytes and T-subsets were not significant between before and after immunotherapy. These findings suggest that serum IgE, skin-test reactivity index and Fcε receptor-bearing lymphocytes in peripheral blood could be useful parameters in assessing the efficacy of immunotherapy with extracts of multi-allergens.

강자성체에서 상전이의 수치적 모의실험

유대영,양해정,양회룡,정진,최승평,장차익 조선대학교 기초과학연구소 2001 自然科學硏究 Vol.24 No.-

It is difficult to explain the magnetic phase transition with classical physics in Curie temperature. We studied qualitatively with the well known Ising model in statistical mechanics. We simulated systems of finite size by Monte Carlo methods with Metropolis algorithm. Thus we could estimate the values of quantities such as the magnetization(m) per spin the specific heat(c) per spin the magnetic susceptibility(χ) per spin, and the mean energy per spin compare with the numerical values in infinitive size. The quantities of c, m, χ, e varied rapidly within 2.3∼2.4 temperature at J/k units. Those values show that the critical temperature can be within the range. The hysteresis loop was obtained with this model.

Testosterone에 의한 승홍의 신독성 유발에 의한 연구

김회영,허근,김석환,최종원 東亞大學校 附設 生活科學硏究所 1993 생활과학연구 논문집 Vol.1 No.-

Mercuric chloride, inorganic compound, is one of the most important drugs that has been used in the field of argriculture, antisyphilitica and anticeptics, but it is not used clinically at present. We have studied the effect of testosterone on the mercuric chlorideinduced nephrotoxicity. Renal lipid peroxide concentration of male rat treated with mercuric chloride was significantly increased in comparison with that of the female rat, it showed similar effects on testosterone pretreatment. Changes in renal catalase and glutathione peroxidase activities were not siginificantly different in testosterone-treated groups. But, renal xanthine oxidase and aldehyde oxidase activities of testrosterone-treated group given mercuric chloride significantly increased in comparison with that of the testosterone-treated alone. Animals treated with testosterone prior to mercuric chloride showed more severe damage on histological observations than those treated with testosterone only. Consequently, we suggest that the mercuric chloride-induced nephrotoxicity might be renal lipid peroxide generating enzyme system by testosterone.

Head-Space SPME에 의한 생체시료 중 메스암페타민류 분석(Ⅲ)

박미정,최상길,손행자,최화경,최영남,허상철,박종서,정희선 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

공공체육시설 운영효율화를 위한 개선방안

고정훈,정경희,박영표,최형원 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.1

The purpose of this study was to provide some improvements for management efficiency of the public sports facilities by analyzing customer satisfaction and opinion of the employers about management system of the public sport facilities. To perform the purpose, this research surveyed 395 customer and 50 employers from 4 cities in Jeollabukdo province. The survey instrument was self developed based on information from prior studies conducted by Hur(1997), Park(1996) and Lee(2001). The statistics analysis method adapted One-way ANOVA in SPSSWIN 10.0 Program The conclusion of this study are as follows. First, The analysis of customer satisfaction. Customer satisfaction level with public sports facilities was higher in the public management facilities than consigned facilities. Especially part of management service. But in indoor gymnasium, consigned facilities was higher. Second, The analysis of Repurchasing and Recommendation behavior. Repurchasing and Recommendation behavior level with public sports facilities was higher in the public management facilities than consigned facilities. Third, In the investigation of employers opinion about management system of the public sports facilities. For the management efficiency of the public sports facilities, organizational restructuring is necessary. The structure of the public sports facilities should be redesigned to decentralize decision-making function. The local sports administrators should have considerable permissive powers. So they can act independently ; they can enable, support and encourage self-helf and initiative. The consignment of the facility management of the public sports facilities such as maintenance and guarding is also needed, finally to enhance the efficiency of the public sports facilities, they are needed to management expert, reinforce the public relations function, marketing strategy, responsible management system, and so on.

Iron Nitrilitriacetate(FeNTA)의 신 조직 손상에 관한 연구

천현욱,김회영,김동영,최종원 慶星大學校 環境問題硏究所 1994 環境硏報 Vol.4 No.1

Nitrilotriacetic acid(NTA) is a synthetic amino polycarboxylic acid chelating agent. It has a high affinity for aluminium and rare earth and alkali earth metals. NTA has been used various countries as a substitute for polyphosphates in houshold detergents to reduce or reverse eutrophication. Renal lipid peroxide concentration of mouse on kidney treated with FeNTA was significantly increased in comparison with that of the normal. Blood urea nitrogen(BUN) level and urinary γ-glutamyltransferase(γ-GT) activity of mice given FeNTA significantly increased in comparison with that of control. But urinary lactate dehydrogenase(LDH) activity was not affected any other conditions. Renal protein bound-SH and glutathione concentration of male mice given FeNTA significantly decreased in comparison with that of control mice. These result suggest that nephrotoxicity of FeNTA caused proximals tubule necrosis on kidney of male mice.

백서피부의 화상 손상에 따른 효소조직화학적 변화

조백현,김영길,허우회,최석현 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.2

The purpose of this study was to investigate the sequential changes of enzyme activities in severely burned skin of rat at intervals of 2 hours, 8 hours, 1 day and 3 days by histochemical methods. The results obtained were as follows. 1. Acid phosphatase activity was found to be transient increase in dermis and mild decrease in epidermis, hair follicle and sebaceous gland at 2 hour after burning. The activity was not seen in skin after 8 hours. 2. Alkaline phosphatase was found to be decreased in blood vessel and dermal papillae of hair follicle at 2 hour after burning and then was progressively decreased after 8 hours. 3.α-naphtyl acetate esterase activity was found to be decreased in epidermis, dermis and hair follicle at 2 hours after burning and was not seen in skin after 8 hours. There was no significant changes in sebaceous gland after burning.

A New Estimation Algorithm for Directional Fields of Fingerprint using Mathematical Morphology

Young Hoe Kim,Min Seop Bae,Tae Young Choi 대한전자공학회 2005 ITC-CSCC :International Technical Conference on Ci Vol.2005 No.1

Synthesis of catechol‐conjugated chitosan and its application as an additive for cement mortar

Choi Hoe Young,Bae Sung‐Ho,Choi Se‐Jin,Ko Haye Min 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.1

We synthesized catechol-conjugated chitosan (CCC) to study its usefulness as a construction material additive in cement mortar. The degree of catechol conjugation (DOCcat) of the synthesized CCC was determined to be approximately 14% by UV–vis and 1H NMR spectroscopy. Furthermore, the hydroxyl and amine groups in CCC could play a crucial role in hydrogen bonding, metal coordination, and cross-linking processes via interaction with adducts from cement mortar. In this study, we observed an improvement in the compressive strength and absorption rate, suggesting that CCC is a promising candidate for high-performance cement mortar.