High efficient rice transformation method by modifying co-cultivation conditions

Hyang-Mi Park,Yul-Ho Kim,Tae-Young Hwang,Chang In Yang,Ji Ung Jeung,Young Chan Cho,Kyoung Ho Kang,Chung-Kon Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5㎎/ℓ L-cysteine, 1mM sodium thiosulfate, 1mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5㎎/ℓ silver nitrate). Co-cultivation temperature (23. 5℃ for 1 day, 26.5℃ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

태양열 온수기의 효율향상에 대한 연구

정낙규,김진홍,오율권,김창오,강승현 조선대학교 에너지.자원신기술연구소 2002 에너지·자원신기술연구소 논문지 Vol.24 No.2

One of the ways to reduce abroad dependence for energy is to utilize solar energy when we are now confronted with not only insufficiency of natural resources but also the serious problems of environmental pollution. Therefore, full attention must now be paid to the development of solar energy system as alternative energy. To achieve this, solar hot water supply system was established at Chosun university, Gwang-ju, and experimental data were obtained based on meterological data and solar radiation. This study has been used the monthly mean values of the daily measurements obtained by data acquisition software Labview. This study shows Gwang-ju is a good region for the utilization of solar energy.

Effect of Annealing on Hysteresis and Eddy Current Components of Iron Loss in Non-Oriented Steels

Song,Chang-Yul,Kang,Yi-Kug,辛龍德 圓光大學校 1995 論文集 Vol.29 No.2

小型 變壓器, 小型모터와 같은 電氣用品의 磁性材料로 가장 많이 使用되고 있는 浦港製鐵 無方向性 電氣銅板 PN-18, 20, 30, 60등을 燒鈍의 SRA(Stress Relief Annealing) 工程을 거쳤을 때, 鐵損[W], 透磁率[μ] 및 磁氣誘導[B]등의 磁氣的 特性 變化를 實驗·調査하였다. 燒鈍 後의 鐵損은 1.5[T] 50Hz와 1.0[T] 50Hz에서 各各 3.071∼7.819[W/kg]이 2.88∼5.492[W/kg]으로, 1.377∼3.988[W/kg]이 1.213∼2.134[W/kg]로 顯著히 減少하지만 磁氣誘導는 微細한 變化를 보였다. 따라서, 燒鈍의 SRA 工程은 無方向性 電氣銅板의 鐵損 및 磁氣的 特性에 重要한 變化를 일으킨다. This paper focuses on results of relative permeability [μ], core less[W] and magnetic induction [B] measurements on some of the most commonly used core materials(PN-18, 20, 30, 60, Pohnag Iron & Steel Co., Ltd.) in case of Stress Relief Annealing(SRA). Results of magnetic induction[B] showed weak variations but core loss reduced strongly after SRA. Core loss reduced from 3.071∼7.819[W/kg] and 1.377∼3.988[W/kg] to 2.88∼5.492[W/kg] and 1.213∼2.134[W/kg] at 1.5[T] 50Hz and 1.0[T] 50Hz respectively after SRA. This SRA process leads to significant changes in magnetic properties and core loss of non-oriented silicon steel sheet.

Graphical and SERS dual-modal identifier for encoding OBOC library

Kang, Homan,Koh, Yul,Jeong, Sinyoung,Jeong, Chulhwan,Cha, Myeong Geun,Oh, Min-Hye,Yang, Jin-Kyoung,Lee, Hyunmi,Jeong, Dae Hong,Jun, Bong-Hyun,Chang, Hyejin,Kim, Yong-Kweon,Lee, Yoon-Sik Elsevier 2020 Sensors and actuators. B, Chemical Vol.303 No.-

<P><B>Abstract</B></P> <P>‘Split-and-mix’ synthetic methods are widely utilized to prepare extensively large numbers of combinatorial libraries. For this, a simple and reliable encoding/decoding method is required to identify a lead compound, especially when it comes to combinatorial peptide/peptoid libraries. Here, we report a simple, efficient, and reliable on-bead peptoid ligand identification method using dual-modal identifiers (DM-IDs), which have a graphical pattern and a Raman signal. The DM-ID was designed to encode the sequence and the type of peptoid side chain by the number of holes and the surface-enhanced Raman scattering (SERS) signals, respectively. Using this dual encoding strategy, 5832 (18<SUP>3</SUP>)-individual peptides/peptoids can be encoded with 3 holes and 18 Raman label compounds. In addition, the method can be easily extended to encode more than one hundred billion ligands by expanding to 9 patterns with 3 holes in binary code. After pentapeptoids were encoded with DM-IDs during solid phase synthesis, the peptoid’s affinity towards streptavidin is successfully evaluated, and the on-bead peptoid sequence is determined by the graphical patterns and the SERS signatures of the DM-IDs. We believe that this encoding method using DM-IDs is a promising tool to construct one-bead-one-compound (OBOC) combinatorial libraries for drug discovery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> On-bead peptoid ligand identification method using dual-modal identifiers (DM-IDs) is demonstrated. </LI> <LI> The DM-ID with graphical (hole structure) and optical (SERS spectrum) codes is successfully fabricated. </LI> <LI> The DM-ID is designed to dictate the sequence of peptoid ligand and the type of side chain. </LI> <LI> More than one hundred billion (18<SUP>9</SUP>)-individual ligands can be encoded with 9-hole patterns and 18 Raman label compounds. </LI> </UL> </P>

해면동부 내경동맥의 외상성 병변과 합병된 지연성 비출혈 : Reprot of 2 Cases

강창구,이동열,박윤규,이영우 대한신경외과학회 1982 Journal of Korean neurosurgical society Vol.11 No.3

A few delayed epistaxis was reported, and it is an unusual complication of closed head injury. We experienced 2 cases of delayed epistaxis and monoocular blindness by cavernous vascular lesions (traumatic aneurysm and C.C.F.). We confirmed the vascular lesions by carotid angiography and Brain C-T. They were treated by carotid ligation and carotid ligation with embolization, and we met with good results. We present 2 cases of the delayed epistaxis complicated by traumatic lesions of cavernous internal carotid artery, and also the literatures were reviewed.

An Off-Site Consequence Modeling for Accident Using Monte Carlo Method

Chang Sun Kang,Sae Yul Lee Korean Nuclear Society 1984 Nuclear Engineering and Technology Vol.16 No.3

원자력발전소 사고 후 그 위험도를 평가하는 새로운 방법으로 몬테칼로 방법을 제시한다. 본 연구에서는 발전소 주위의 주민에게 주는 방사선의 영향을 평가하기 위하여 공기중의 확산계산에 부지에서 측정한 기상조건을 직접 사용하고 있다. 사고가 일어나는 순간에서의 화산조건은 주어진 기상자료로부터 분석된 pdf에 의하여 결정되고 그이후의 조건(풍향, 풍속, 안정도)은 마르코프 조건을 만족시킨다고 가정하였다. 예제로써 KNU-1의 냉각재 상실사고를 분석한 절과 50마일내의 주민이 받는 선량은 50퍼센트 신뢰도를 갖고 200 man-Sv이다. A new medal is presented in order to evaluate the risk from a nuclear facility following accidents directly combining the on-site meteorological data using the Monte Carlo Method. To estimate the radiological detriment to the surrounding population-at-large (collective dose equivalent), in this study the probability distribution of each meteorological element based upon on-site data is analyzed to generate atmospheric dispersion conditions. The random sampling is used to select the dispersion conditions at any given time of effluent releases. In this study it is considered that the meteorological conditions such as wind direction, speed and stability are mutually independent and each condition satisfies the Markov condition. As a sample study, the risk of KNU-1 following the large LOCA was calculated, The calculated collective dose equivalent in the 50 mile region population from the large LOCA with 50 percent confidence level is 2.0$\times$10$^2$ man-sievert.

Conceptual Design for HTS Coil in Superconducting Electromagnet for Maglev

Chang Young Lee,Jung Min Jo,Bubyoung Kang,Young Jea Han,Duck Kweon Bae,Yong Soo Yoon,Yoon Do Chung,Sung Yul Chu,Young Jin Hwang,Tae Kuk Ko IEEE 2011 IEEE transactions on applied superconductivity Vol.21 No.3

<P>This study deals with a conceptual design for a high temperature superconductor (HTS) coil fabricated with YBCO wire in a prototype HTS-electromagnet (HTS-EM) model for the electromagnetic suspension (EMS)-based Maglev. Because the size of the HTS coil and the power it consumes during operation are very critical factors in designing the HTS-EM, we focused on the number of coil turns and the operating conditions needed to generate the required magneto-motive force (MMF) effectively. The winding geometry of the sample HTS coil with the largest Ic value was selected for use in this study. To determine the operating condition corresponding to the number of coil turns, we produced the operating profile for the load current of DC current source and the Ic value of HTS coil. From the profile, the optimal number of coil turns to minimize the operating power is determined to be in the range of 1200 to 1400 turns. And the HTS coil should be operated with the current of 37 to 43 A at the cooling temperature of 73 to 76 K.</P>

EXTRACORPOREAL SHOCKWAVE LITHOTRIPSY MONOTHERAPY WITH THE NEW DORNIER MPL-9000 LITHOTRIPTER

Chang, Jin Ho,Kang, Heon Joong,Lee, Chang Kyu,Choi, Jong Geun,Kim, Jong Chul,Rhew, Hyn Yul 고신대학교 의학부 1992 高神大學校 醫學部 論文集 Vol.8 No.1

현재 12가지가 넘는 다양한 종류의 쇄석기가 사용되고 있으나 Dornier MPL-9000은 요로결석과 담석의 치료를 위해 최근 개발된 제3세대 쇄석기로 타쇄석기에 비해 높은 성공율 및 안정성이 보고되고 있다. 본교실에서는 1990년 12월부터 1992년 4월까지 요로 결석 환자 155례에 대하여 Spark gap type의 Dernier MPL-9000쇄석기를 이용하여 체외충격파 쇄석술을 시행하여 얻은 결과를 보고하는 바이다. 결석의 위치는 신결석 95례(61.2%), 요관결석 57례(36.8%), 방광결석 3례(1.9%)였으며, 크기는 0.5cm부터 4.8cm까지로 평균 1.4cm이었다. 시술횟수는 한 환자에서 1-5회로 총 217회 였으며 평균 1.4회였다. 전체 결석 분쇄율은 1회 쇄석술후 71.6%, 추가 재시술후 98.1%의 성공율을 보였으며 1회 쇄석술후 위치별 완전 분쇄율은 신결석이 75.7%였고 요관결석이 70.1%였고 크기별 완전 분쇄율은 1cm 미만 결석87%, 1cm에서 2cm결석 73%, 2cm이상에서 5%였다. 보조 시술로는 요관결석에 대한 push-up 29례(18.7%), 요관 카테터 우회 3례(1.9%), 신장결석에 대한 요관 부목 유치 2례(1.3%)를 시행하였다. 시술시 통증이 있은 86회(39.6%)중 진통제를 필요로 한 경우가 36회(16.6%)였으나 치료를 중단한 예는 없었다. 합병증으로는 육안적 혈뇨 115례(74.2%), 요관 돌길형성 24례(15.5%),산통 10례(6.4%) 오심 11례(7.0%) 혈압변화 3례(1.9%), 발열 3례(1.9%)등이었다. 위의 결과로 요로결석에서 Dornier MPL-9000쇄석기를 이용한 단독치료는 안전하고 효과적인 우선적 치료방법으로 생각된다.

A Retrospective Study on Radiotherapy and Radiochemotherapy in Esophageal Cancer

Chang Min Kim,Weon Seon Hong,Jhin Oh Lee,Tae Woong Kang,Yong Hyon Kim,Chul Gu Cho,Kyoung Hwan Koh,Seong Yul Yoo 대한내과학회 1988 The Korean Journal of Internal Medicine Vol.3 No.1

고혈압 초진시 선택된 초치료처방 및 임상검사항목의 분석 : 1988년 전후의 가정의학과 전공의 비교 및 1988년 이후의 가정의학과와 내과 전공의의 비교 comparison between FM residents before and after 1988 and between FM residents and IM residents after 1988

강재헌,유선미,오현주,전옥희,유태우,허봉렬,김창엽 대한가정의학회 1992 Korean Journal of Family Medicine Vol.13 No.5