Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

인플루엔자 바이러스에 對한 血淸學的 및 病毒學的 調査

白承福,張起峰,金慶浩 國立保健硏究院 1972 국립보건연구원보 Vol.9 No.-

Aerosol delivery of eukaryotic translation initiation factor 4E-binding protein 1 effectively suppresses lung tumorigenesis in K-ras^LA1 mice

Chang, S-H,Kim, J-E,Lee, J-H,Minai-Tehrani, A,Han, K,Chae, C,Cho, Y-H,Yun, J-H,Park, K,Kim, Y-S,Cho, M-H Nature America, Inc. 2013 Cancer gene therapy Vol.20 No.6

Conventional radiotherapy or chemotherapy for the long-term survival of patients with lung cancer is still difficult for treatment in metastatic and advanced tumors. Therefore, the safe and effective approaches to the treatment of lung cancer are needed. In this study, the effect of delivered eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1) on lung cancer progression was evaluated. Recombinant adeno-associated virus (rAAV)-M3/4E-BP1 was delivered into 6-week-old K-ras<SUP>LA1</SUP> lung cancer model mice through a nose-only inhalation system twice a week for 4 weeks. Long-term repeated delivery of 4E-BP1 effectively reduced tumor progression in the lungs of K-ras<SUP>LA1</SUP> mice. Reduction of eIF4E by overexpression of 4E-BP1 resulted in suppression of cap-dependent protein expression of basic fibroblast growth factor (bFGF or FGF-2) and vascular endothelial growth factor (VEGF). In addition, delivered 4E-BP1 inhibited the proliferation of lung cancer cells in K-ras<SUP>LA1</SUP> mice model. Our results suggest that long-term repeated viral delivery of 4E-BP1 may provide a useful tool for designing lung cancer treatment.

NO₂Sensing Properties of CuPc and H₂Pc LB Films

Kwon, H.J.,Lee, Y.-J.,Chang, Y.K.,Kim, J.-D. 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

Characteristics of CuPc and H_(2)Pc Langmuir-Blodgett(LB) films were examined and their NO_(2) sensing properties were investigated on a gas sensor using 9 MHz quartz crystal microbalance(QCM). The monolayer behavior of H_(2)Pc in air-water interface was observed by inspecting its UV absorption spectra as the surface pressure was increased. On ellipsometry measurement for CuPc and H_(2)Pc LB films, the thickness of H_(2)Pc LB film was almost twice as thick as that of CuPc. X-ray photoelectronic spectroscopy (XPS) spectra of CuPc LB film showed that Cu2p(1/2) and Cu2p(3/2) peak shifts arised from new bond formation by NO_(2) adsorption. Both CuPc and H_(2)Pc LB film produced significant frequency changes in low ppm range of NO_(2).

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

체위변경이 Furosemide 이뇨중 Aldosterone 배설에 미치는 영향

성호경,고주환,이장규,박기복,이경희,장호조,서성희,안희중,신옥자 주식회사 녹십자 1977 녹십자의보 Vol.5 No.2

韓國人의 風疹 바이러스 血球凝集抑制抗體調査

Patwory, K.M.,金慶浩,徐俊錫,張起峰,鄭泰和 대한감염학회 1972 감염 Vol.4 No.1

The Hemagglutination-Inhibition (H-I) antibodies against rubella virus among Korean in 1972 were studied. The H-I antibody possess rate and antibody titers by age group and by area were analyzed. The data in Table's 1,2 and 3 summarize the results of H-I testing of 160 serum specimens. Age group 16~20 and 31~40 gives somewhat higher positiveness than the other age group. The age group 1 year has the lowest positivity. This interaction may be also significant due to a certain extent of different type of titers with titer 1 : 10 producing the highest number of positiveness and specifically with the age group of 11~15 and 21~30. The rural and urban areas people do not seem to have differences in terms of their titer positiveness and same is true in their interaction with age titer revealing on insignificant test. Therefore, in summary we conclude 1. The titers differ significantly in producing positiveness. Titer 1 :10 has the highest of producing such with very little of 1 : 320 and 1 : 640. 2. In general the age groups differ in terms of positiveness by titer but the degree of positiveness do not differ amongst tested population over 16 year of age groups very significantly. Therefore, the effect of titers beyond this age group population would be of similar magnitude. 3. The interaction between titer and age group seem to have a very strong impact on "positiveness" revealing the fact that individual aged will always bear significant variation in positiveness. 4. No significant variation in terms of positivencess could be observed amongst the rural and urban population from this experimental observation and same could be said about their being rural or urban with age or titer.

A型 인플루엔자에 對한 血淸疫學的 調査

白承福,張起峰,崔憲 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

소아 쭈쭈가무시병환자 28명의 임상상 및 유병기간에 따른 간접면역형광항체가의 변동상

박병규,오영균,윤희상,염명걸,유한욱,김재형,강환섭,김익상,장우현,김승환 대한감염학회 1993 감염 Vol.25 No.2

During the autumn of 1990 , we had investigated the clinical featuresw and followed the change in the direct immunofluorescent antibody(IFA) titers in 28 children with scrub typhus who resided in or near Chinju, Korea. All the children except one were diagnosed by the peak IFA titers of 1:160 or higher. The results were as follows: 1) The clinical features of our children were not different, to a large degree, from those of adults ever described in Korea literatures. But the frequence rates of skin rash, eschar and conjunctival injection were higher, those of proteinuria and hematuria were lower than those in adults and the characters of rash in our children were also quite different(faces were frequently the first site of eruption and even palms and soles were involved occasionally, initial bright red color of rash was changed into brown color during the acute phase). 2) IFA titers rose rapidly during the 2nd or 3rd week of illness and then formed plateau. When we suggest IFA titers of 1:180 or higher as the diagnostic criteria, the seropositivity of our children was 5/7(75%) in the 1st week and over 95% in the 2nd through 5th week of illness, and suggest IFA titers of 1:160 or higher,then the seropositivityin the 1st, 2nd and 3rd through 5th week were 3/7(43%), 23/31(74%) and over 95%,respectively. 3) IFA titers in 6-8 months after disease onset were 1:80(13 children) or 1:20( 7 children), and in one and half year (our longest follow-up period), 1:20 (3 children) showing that IFA lasts longer than one and half year after sickness. 4) From the above data (3) and the fact that majority of cases of scrub typhus occur in the autumn season in Korea, IFA titers of 1:40-1:160 should be used as the diagnostic criteria in order to distinguish between the present and past infections.

Comparison between a reanalyzed product by 3-dimensional variational assimilation technique and observations in the Ulleung Basin of the East/Japan Sea

Kim, Y.H.,Chang, K.I.,Park, J.J.,Park, S.K.,Lee, S.H.,Kim, Y.G.,Jung, K.T.,Kim, K. Elsevier 2009 Journal of marine systems Vol.78 No.2

Reanalyzed products from a MOM3-based East Sea Regional Ocean Model with a 3-dimentional variational data assimilation module (DA-ESROM), have been compared with the observed hydrographic and current datasets in the Ulleung Basin (UB) of the East/Japan Sea (EJS). Satellite-borne sea surface temperature and sea surface height data, and in-situ temperature profiles have been assimilated into the DA-ESROM. The performance of the DA-ESROM appears to be efficient enough to be used in an operational ocean forecast system. Comparing with the results from Mitchell et al. [Mitchell, D. A., Watts, D. R., Wimbush, M., Teague, W.J., Tracey, K. L., Book, J. W., Chang, K.-I., Suk, M.-S., Yoon, J.-H., 2005a. Upper circulation patterns in the Ulleung Basin. Deep-Sea Res. II, 52, 1617-1638.], the DA-ESROM fairly well simulates the high variability of the Ulleung Warm Eddy and Dok Cold Eddy as well as the branching of the Tsushima Warm Current in the UB. The overall root-mean-square error between 100 m temperature field reproduced by the DA-ESROM and the observed 100-dbar temperature field is 2.1 <SUP>o</SUP>C, and the spatially averaged grid-to-grid correlation between the two temperature fields is high with a mean value of 0.79 for the inter-comparison period. The DA-ESROM reproduces the development of strong southward North Korean Cold Current (NKCC) in summer consistent with the observational results, which is thought to be an improvement of the previous numerical models in the EJS. The reanalyzed products show that the NKCC is about 35 km wide, and flows southward along the Korean coast from spring to summer with maximum monthly mean volume transport of about 0.8 Sv in August-September.