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이두형,최병열,장원철,서길수,이태진 영남대학교 공업기술연구소 1999 工業技術硏究所論文集 Vol.27 No.1
Mesoporous MCM-41 exibits a hexagonal arrangement of uniform mesopores whose dimensions may be engineered in the range of ∼20Å to greater than 100Å. The MCM-41 typically have high surface areas above 1000m2/g, and then capacities of MCM-41 to accommodate the larger hydrocarbon is still higher than the general molecular sieve. At the present, many surfactants as organic templates have been proposed for various formations of these MCM-41. In this study, we synthesized mesoporous MCM-41 which has a spherically hexagonal structure. Also, the pore size of MCM-41 could be changed by the control of alkyl chain length of surfactants.
공업화학 촉매 / 반응공학 : 변경된 Zeolite 촉매상에서 o - toluidine 의 N - 알킬화 반응에 관한 연구
최병렬(Byung Yul Choi),장원철(Won Chul Chang) 한국화학공학회 2000 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.38 No.2
A study of reaction processes using solid catalysts has drawn attention of domestic dyestuff intermediate manufacturers. From this viewpoint, N-alkylation of o-toluidine with ether was conducted on H/zeolite-X catalysts modified with K, Cs, K-Mg, and MgO, respectively. While H/zeolite-X gave rise to maximum conversion at 300℃ those zeolite-X catalysts of lowered acidity due to modification by alkali metal exhibited maximum conversions at relatively higher temperatures: 320℃ for K/zeolite-X and Cs/zeolite-X, 350 ℃ for K-Mg/zeolite-X, 400 ℃ for MgO/zeolite-X. For H/zeolite-X and zeolites modified by ion exchange with K, Cs, or K-Mg, as the reaction temperature increased in 300-420 ℃ range, the activity of N-alkylation forming N-ethyl-o-toluidine decreased rapidly whereas the activity of C-alkylation forming 6-ethyl-o-toluidine increased. Meanwhile, for the same temperature increase, MgO/zeolite-X having higher basicity showed relatively much smaller variation of N-alkylates or C-alkylates yield. Those zeolite-X catalysts modified with alkali metals including Cs selectively catalyzed side-chain alkylation producing N-alkylates predominantly. Maximum conversions were brought about at o-toluidine: ether mole ratio of 1 : 4 in the reaction mixture, and lowered acidity of the catalysts modified by ion exchange with alkali metals were responsible for the increase of reaction Temperature for maximum activity, relative to H/zeolite-X. In the case of Cs/ zeolite-X catalysts, Cs loading of 1 wt% gave rise to the highest conversion of o-toluidine.
Improved baculovirus vectors expressing barnase using promoters from Cotesia plutellae bracovirus
Choi, Jae Young,Kim, Yang-Su,Wang, Yong,Kang, Joong Nam,Roh, Jong Yul,Shim, Hee Jin,Woo, Soo-Dong,Jin, Byung Rae,Je, Yeon Ho Springer-Verlag 2009 Molecules and cells Vol.28 No.1
<P>The goal of this study was to create a novel baculovirus expression system that does not require recombinant virus purification steps. Transfection of insect cells with transfer vectors containing barnase under control of the Cotesia plutellae bracovirus (CpBV) promoters ORF3004 or ORF3005 reduced cell growth. Co-transfection with bApGOZA DNA yielded no recombinant viruses and non-recombinant backgrounds. To further investigate the detrimental effects of barnase on insect cells, two recombinant bacmids harboring the barnase gene under control of the CpBV promoters, namely bAcFast-3004ProBarnase and bAcFast-3005ProBarnase, were constructed. While no viral replication was observed when only the recombinant bacmids were transfected, recombinant viruses were generated when the bacmids were co-transfected with the transfer vector, pAcUWPolh, through substitution of the barnase gene with the native polyhedrin gene by homologous recombination. Moreover, no non-recombinant backgrounds were detected from unpurified recombinant stocks using PCR analysis. These results indicate that CpBV promoters can be used to improve baculovirus expression vectors by means of lethal gene expression under the control of these promoters.</P>
Fast and efficient generation of recombinant baculoviruses by in vitro transposition
Choi, Jae Young,Kim, Yang-Su,Wang, Yong,Tao, Xue Ying,Liu, Qin,Roh, Jong Yul,Woo, Soo Dong,Jin, Byung Rae,Je, Yeon Ho Springer-Verlag 2012 Applied microbiology and biotechnology Vol.96 No.5
<P>A novel recombinant bacmid, bEasyBac, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. In bEasyBac, attR recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. Moreover, the extracellular RNase gene from Bacillus amyloliquefaciens, barnase, was expressed under the control of the Cotesia plutellae bracovirus early promoter to negatively select against the non-recombinant background. The bEasyBac bacmid could only replicate in host insect cells when the barnase gene was replaced with the gene of interest by in vitro transposition. When bEasyBac was transposed with pDualBac-EGFP, the resulting recombinant virus, AcEasy-EGFP, showed comparable levels of EGFP expression efficiency to the plaque-purified recombinant virus AcEGFP, which was constructed using the bAcGOZA system. In addition, no non-recombinant backgrounds were detected in unpurified AcEasy-EGFP stocks. Based on these results, a high-throughput system for the generation of multiple recombinant viruses at a time was established.</P>
Analysis of promoter activity of selected Cotesia plutellae bracovirus genes
Choi, Jae Young,Kwon, Soo-Jin,Roh, Jong Yul,Yang, Tae-Jin,Li, Ming Shun,Park, Beom-Seok,Kim, Yonggyun,Woo, Soo-Dong,Jin, Byung Rae,Je, Yeon Ho Microbiology Society 2009 The Journal of general virology Vol.90 No.5
<P>In a previous study, we cloned 27 discrete genome segments of Cotesia plutellae bracovirus (CpBV) and provided the complete nucleotide sequences and annotation. Seven putative coding regions were predicted from one of the largest segments, CpBV-S30. The activity of promoters associated with six predicted ORFs from this segment were investigated using both transient and baculovirus expression assays with enhanced green fluorescent protein as a reporter gene. CpBV promoters showed activity earlier than the polyhedrin promoter and the activity of some of these promoters was superior to that of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ie-1 promoter in the baculovirus expression assays. The promoter of ORF3004 showed the highest level of activity in insect cells, exhibiting 24 % of the activity obtained with the AcMNPV polyhedrin promoter in Sf9 cells. In Spodoptera exigua larvae, the ORF3006 promoter showed the highest activity, with about 35 % of the activity measured with the polyhedrin promoter. In addition, analysis of the ORF3006 promoter revealed that the region between -382 and -422 from the translation start point was critical for activity of this promoter. These results suggest that the CpBV-S30 promoters characterized here could be useful tools in a variety of biotechnological applications, such as gene expression analyses and insecticide development.</P>
( Yul Hee Cho ),( Seok Hui Kang ),( Yaeni Kim ),( Myung Hyun Lee ),( Gun Hee An ),( Byung Ha Chung ),( Bum Soon Choi ),( Chul Woo Yang ),( Yong Soo Kim ),( Yeong Jin Choi ),( Cheol Whee Park ) 대한신장학회 2013 Kidney Research and Clinical Practice Vol.32 No.3
Background: Nephrotic syndrome (NS) and proteinuria are uncommon, often unrecognized manifestations of graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation (HSCT). Only a few isolated case reports and case series involving smaller number of patients who developed NS after HSCT have been published. Methods: We reviewed the renal histopathological examination findings and clinical records of 15 patients who developed proteinuria after HSCT at Seoul and Yeouido St. Mary′s Hospital (Seoul, Korea). We also measured the anti-PLA2R antibodies (M-type phospholipase A2 receptor) in the serum samples from the seven patients at the time of renal biopsy. Results: All patients had GVHD. The most common indication for biopsy was proteinuria (41 g/day), with nine patients having nephrotic range proteinuria. The most common histopathological finding was membranous nephropathy (MN; n ¼ 12). Other findings were membranoproliferative glomerulonephritis, C1q nephropathy, and diabetic nephropathy. Eleven patients were treated with immunosuppressive agents, and three patients were treated only with angiotensin II receptor blocker. The overall response rate, including complete remission (urinary protein level o0.3 g/day) and partial remission (urinary protein level ¼ 0.31?3.4 g/day), was 73%. The mean follow-up period was 26 months, and none of the patients developed end-stage renal disease. All of the seven patients with MN had negative findings for anti-PLA2R antibodies, measured using an enzyme-linked immunosorbent assay kit. Conclusion: In this study the findings of 15 renal biopsies were analyzed and to our knowledge this is the largest clinicopathological study of GVHD-related biopsy-proven nephropathy. Approximately 80% of the patients were MN and 73% responded either partially or completely to immunosuppressive treatment. Currently, there is an increase in the incidence of GVHD-mediated renal disease, and therefore, renal biopsy is essential for diagnosing the nephropathy and preventing the progression of renal disease.
Sequence and gene organization of 24 circles from the Cotesia plutellae bracovirus genome
Choi, Jae Young,Kwon, Soo-Jin,Roh, Jong Yul,Yang, Tae Jin,Yoon, Sook Hee,Kim, Heebal,Li, Ming Shun,Park, Beom-Seok,Woo, Soo-Dong,Jin, Byung Rae,Kim, Yonggyun,Je, Yeon Ho Springer-Verlag 2009 Archives of virology Vol.154 No.8